蝙蝠蛾拟青霉菌Cs-4菌株对腺嘌呤造成肾脏和睾丸损伤的恢复作用
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摘要
本文用腺嘌呤建立肾脏和睾丸损伤的小鼠模型,探索蝙蝠蛾拟青霉菌Cs-4菌株对腺嘌呤造成的肾脏和睾丸损伤的恢复作用。将小鼠随机分为空白组、模型组和蝙蝠蛾拟青霉菌Cs-4菌粉的5个剂量组(350mg/kg、550mg/kg、750mg/kg、1 500mg/kg、3 000mg/kg),连续给予相应药物21d,对各组小鼠的肾脏、睾丸通过HE染色、Masson染色,进行组织病理学检查,测定各组小鼠血清中尿素氮(BUN)、肌酐(Cr)、促卵泡刺激素(FSH)、黄体生成素(LH)含量,测定睾丸组织匀浆中的酸性磷酸酶(ACP)、琥珀酸脱氢酶(SDH)和乳酸脱氢酶(LDH)水平。结果表明,蝙蝠蛾拟青霉菌Cs-4菌株具有使小鼠体重逐渐恢复上升趋势、使受损的肾脏和睾丸组织结构恢复的作用,能使BUN、Cr、FSH、LH、ACP、SDH和LDH水平趋于正常化。
A mouse model of kidney and testis injury was established using adenine to explore the recovery effect of fermented powder products of Paecilomyces hepiali Cs-4 on adenine-induced kidney and testis damage. The mice were randomly divided into control group, model group, five treatment groups orally treated with different doses of fermented powder products of Paecilomyces hepiali Cs-4(350 mg/kg, 550 mg/kg,750 mg/kg, 1 500 mg/kg, 3 000 mg/kg). The mice were treated daily for consecutive 21 days. The histopathological observation of the kidney and testis of each group of mice was carried out by using HE and Masson staining.The content of BUN, Cr, FSH and LH in blood serum were detected. In addition, the content of lactate dehydrogenase(LDH), acid phosphatase(ACP) and succinate dehydrogenase(SDH) in testis homogenate were detected. The results showed that the body weight of mice gradually recovered and increased in the experimental group treated with fermented powder products of Paecilomyces hepiali Cs-4, manifesting a recovery effect on damaged kidney and testis structure, and normalization of BUN, Cr, FSH, LH, ACP, SDH and LDH levels.
A mouse model of kidney and testis injury was established using adenine to explore the recovery effect of fermented powder products of Paecilomyces hepiali Cs-4 on adenine-induced kidney and testis damage. The mice were randomly divided into control group, model group, five treatment groups orally treated with different doses of fermented powder products of Paecilomyces hepiali Cs-4(350 mg/kg, 550 mg/kg,750 mg/kg, 1 500 mg/kg, 3 000 mg/kg). The mice were treated daily for consecutive 21 days. The histopathological observation of the kidney and testis of each group of mice was carried out by using HE and Masson staining.The content of BUN, Cr, FSH and LH in blood serum were detected. In addition, the content of lactate dehydrogenase(LDH), acid phosphatase(ACP) and succinate dehydrogenase(SDH) in testis homogenate were detected. The results showed that the body weight of mice gradually recovered and increased in the experimental group treated with fermented powder products of Paecilomyces hepiali Cs-4, manifesting a recovery effect on damaged kidney and testis structure, and normalization of BUN, Cr, FSH, LH, ACP, SDH and LDH levels.
引文
Dai RQ,Shen CY,Li XM,Lan JL,Lin SF,Shao AJ,2017.Discussion on related problems of model species of fungus Paecilomyces hepiali.China Journal of Chinese Materia Medica,42(19):3843-3846(in Chinese)
He Y,Cheng ZX,Chen H,Huang M,2014.A comparative study on the effect of tonic“kidney-yang”of Morinda officinalis from different habitats after salt preparation.Lishizhen Medicine and Materia Medica Research,25(4):856-858(in Chinese)
Jiang X,Wang CY,Li LZ,Wang X,Teng LR,Zhou YL,2017.Activity comparison study on Paecilomyces hepiali,Cordyceps militaris and wild Cordyceps sinensis.Journal of Changchun University of Chinese Medicine,33(4):544-547(in Chinese)
Liu J,Yang BS,Jiang GY,2012.Antibiotic activities and chemical composition of mycelia of a strain Paecilomyces hepiali.Journal of Kunming Medical University,33(6):32-34+38(in Chinese)
Park SY,Jung SJ,Ha KC,Sin HS,Jang SH,Chae HJ,Chae WS,2015.Anti-inflammatory effects of Cordyceps mycelium(Paecilomyces hepiali,CBG-CS-2)in Raw264.7 murine macrophages.Oriental Pharmacy&Experimental Medicine,15(1):7-12
Thakur A,Ren H,Zhang HY,Yang T,Chen TJ,Chen MW,2011.Pro-apoptotic effects of Paecilomyces hepiali,a Cordyceps sinensis extract on human lung adenocarcinoma A549 cells in vitro.Journal of Cancer Research&Therapeutics,7(4):421
Wang J,2017.Protective effects of polysaccharides obtained from Paecilomyces hepiali fermentation mycelium in experimental diabetic animal model.PhD Dissertation,Jilin University,Changchun.1-103(in Chinese)
Wang J,Li LZ,Liu YG,Teng LR,Lu JH,Xie J,Hu WJ,Liu Y,Liu Y,Wang D,Teng LS,2016.Investigations on the antifatigue and antihypoxic effects of Paecilomyces hepiali extract.Molecular Medicine Reports,13(2):1861-1868
Wang J,Liu Y,Li L,Qi Y,Zhang Y,Li L,Teng L,Wang D,2017.Dopamine and serotonin contribute to Paecilomyces hepiali against chronic unpredictable mild stress induced depressive behavior in Sprague Dawley rats.Molecular Medicine Reports,16(4):5675
Wang L,Xu J,Li H,Song L,Yu Y,Zhang W,Liu G,Feng C,2014.The complete mitochondrial genome of Paecilomyces hepiali(Ascomycota,Eurotiomycetes).Mitochondrial DNA the Journal of DNA Mapping Sequencing&Analysis,27(2):916-917
Wang N,2010.Protective effects of Paecilomyces hepiali on adriamycin nephropathy in rats.Master Thesis,Shandong University,Jinan.1-39(in Chinese)
Wu Z,Zhang M,Xie M,Dai Z,Wang X,Hu B,Ye H,Zeng X,2016.Extraction,characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali,HN1.Carbohydrate Polymers,137(3):541
Wu ZW,2014.Optimization fermentation,purification,structural elucidation and bioactivity of exopolysaccharides from Paecilomyces hepiali.PhD Dissertation,Nanjing Agricultural University,Nanjing.137-148(in Chinese)
Wu ZW,Lu JW,Wang XQ,Hu B,Ye H,Fan JL,Muhammad Abid,Zeng XX,2013.Optimization for production of exopolysaccharides with antitumor activity in vitro from Paecilomyces hepiali.Carbohydrate Polymers,99:226-234
Xiang M,Tang J,Zou XL,Zhao ZY,Wang YY,Xie SN,2009.βcell protecting and immunomodulatory activities of Paecilomyces hepiali Chen mycelium in STZ induced T1DM mice.The American Journal of Chinese Medicine,37(2):361-372
Xu CY,Li H,Lu ZM,2016.Analysis of the chemical component of Paecilomyces hepiali in submerged culture.Edible Fungi,38(3):64-66(in Chinese)
Xue Z,2017.The effect of“yi shen tong luo”recipe on rat testis SOD,MDA and serum T,FSH,LH of infertility model rats through adenine method.Master Thesis,Henan University of Chinese Medicine,Zhengzhou.1-28(in Chinese)
Yang D,2015.Study on of analgesic effective part of Paecilomyces hepiali and its sustained-release preparations.Master Thesis,Yunnan University of Traditional Chinese Medicine,Kunming.1-75(in Chinese)
Yu SJ,2012.Bioactivities and structure elucidation of exopolysaccharides from fermented broth of Paecilomyces hepiali.PhD Dissertation,Anhui Agricultural University,Hefei.1-65(in Chinese)
Zeng HW,Wang WF,Ma MH,Xu GH,Sun JF,Li WC,Yang YW,2016.Comparison of main bioactive composition and nutritional components among three fermented power commonly used as substitutes for Cordyceps sinensis.Journal of Yunnan Normal University,36(1):66-70(in Chinese)
Zhang XJ,Liu ZJ,Dang YH,Liu JW,Wu S,Wang YN,Li ZL,2013.Study on effects of apigenin on activity of testicular marker enzymes in mice.Journal of Medical Pest Control,29(11):1217-1221(in Chinese)
Zhang ZT,Xie GY,Chen K,Qi MY,Liu HR,2012.Alteration of marker enzymes in adenine-induced impaired testis and the interference of total flavonoids of herba epimedii in rats.Chongqing Medicine,41(7):683-685(in Chinese)
Zheng LF,2017.Study on pharmacodynamics and mechanism of“Jinkui Shenqi”pills in intervention of adenine-induced rat kidney“yang”deficiency model.Master Thesis,Beijing University of Chinese Medicine,Beijing.1-72(in Chinese)
戴如琴,沈崇尧,李晓明,兰江丽,林淑芳,邵爱娟,2017.蝙蝠蛾拟青霉模式菌相关问题的探讨.中国中药杂志,42(19):3843-3846
姜雪,王春月,李兰洲,王雪,滕利荣,周毓麟,2017.蝙蝠蛾拟青霉及蛹虫草与野生冬虫夏草活性比较.长春中医药大学学报,33(4):544-547
刘俊,杨本寿,姜国银,2012.蝙蝠蛾拟青霉的抑菌活性与菌丝体发酵化学组分的研究.昆明医科大学学报,33(6):32-34+38
王娟,2017.蝙蝠蛾拟青霉液体发酵菌丝体多糖对实验性糖尿病保护作用探讨.吉林大学博士论文,长春.1-103
王娜,2010.蝙蝠蛾拟青霉Cs-4对阿霉素肾病大鼠的肾脏保护作用.山东大学硕士论文,济南.1-39
武忠伟,2014.蝙蝠蛾拟青霉胞外多糖的发酵优化、结构鉴定及生物活性研究.南京农业大学博士论文,南京.137-148
何艳,程再兴,陈红,黄枚,2014.不同产地巴戟天盐制后补肾阳功效的比较研究.时珍国医国药,25(4):856-858
许春燕,李恒,陆震鸣,2016.蝙蝠蛾拟青霉发酵菌粉的主要成分分析.食用菌,38(3):64-66
薛铮,2017.益肾通络方对腺嘌呤法不育模型大鼠睾丸SOD、MDA和血清FSH、LH、T的影响.河南中医药大学硕士论文,郑州.1-28
阳丹,2015.蝙蝠蛾拟青霉菌丝体镇痛有效部位及其缓释制剂研究.云南中医学院硕士论文,昆明.1-75
于士军,2012.蝙蝠蛾拟青霉胞外多糖活性和结构的研究.安徽农业大学博士论文,合肥.1-65
曾化伟,王文风,马猛华,徐国华,孙见凡,厉文成,杨亚威,2016.三种替代冬虫夏草发酵菌粉的营养成分和活性成分的比较研究.云南师范大学学报(自然科学版),36(1):66-70
张晓晶,刘子婧,党瑜慧,刘佳微,吴双,汪燕妮,李芝兰,2013.芹菜素对小鼠睾丸标志酶活性的影响.医学动物防制,29(11):1217-1221
张作涛,谢高宇,陈凯,齐敏友,刘浩然,2012.腺嘌呤致大鼠睾丸损伤中标志酶的变化及淫羊藿总黄酮的干预作用.重庆医学,41(7):683-685
郑龙飞,2017.金匮肾气丸加味干预腺嘌呤所致大鼠肾阳虚模型的药效学及作用机理研究.北京中医药大学硕士论文,北京.1-72
He Y,Cheng ZX,Chen H,Huang M,2014.A comparative study on the effect of tonic“kidney-yang”of Morinda officinalis from different habitats after salt preparation.Lishizhen Medicine and Materia Medica Research,25(4):856-858(in Chinese)
Jiang X,Wang CY,Li LZ,Wang X,Teng LR,Zhou YL,2017.Activity comparison study on Paecilomyces hepiali,Cordyceps militaris and wild Cordyceps sinensis.Journal of Changchun University of Chinese Medicine,33(4):544-547(in Chinese)
Liu J,Yang BS,Jiang GY,2012.Antibiotic activities and chemical composition of mycelia of a strain Paecilomyces hepiali.Journal of Kunming Medical University,33(6):32-34+38(in Chinese)
Park SY,Jung SJ,Ha KC,Sin HS,Jang SH,Chae HJ,Chae WS,2015.Anti-inflammatory effects of Cordyceps mycelium(Paecilomyces hepiali,CBG-CS-2)in Raw264.7 murine macrophages.Oriental Pharmacy&Experimental Medicine,15(1):7-12
Thakur A,Ren H,Zhang HY,Yang T,Chen TJ,Chen MW,2011.Pro-apoptotic effects of Paecilomyces hepiali,a Cordyceps sinensis extract on human lung adenocarcinoma A549 cells in vitro.Journal of Cancer Research&Therapeutics,7(4):421
Wang J,2017.Protective effects of polysaccharides obtained from Paecilomyces hepiali fermentation mycelium in experimental diabetic animal model.PhD Dissertation,Jilin University,Changchun.1-103(in Chinese)
Wang J,Li LZ,Liu YG,Teng LR,Lu JH,Xie J,Hu WJ,Liu Y,Liu Y,Wang D,Teng LS,2016.Investigations on the antifatigue and antihypoxic effects of Paecilomyces hepiali extract.Molecular Medicine Reports,13(2):1861-1868
Wang J,Liu Y,Li L,Qi Y,Zhang Y,Li L,Teng L,Wang D,2017.Dopamine and serotonin contribute to Paecilomyces hepiali against chronic unpredictable mild stress induced depressive behavior in Sprague Dawley rats.Molecular Medicine Reports,16(4):5675
Wang L,Xu J,Li H,Song L,Yu Y,Zhang W,Liu G,Feng C,2014.The complete mitochondrial genome of Paecilomyces hepiali(Ascomycota,Eurotiomycetes).Mitochondrial DNA the Journal of DNA Mapping Sequencing&Analysis,27(2):916-917
Wang N,2010.Protective effects of Paecilomyces hepiali on adriamycin nephropathy in rats.Master Thesis,Shandong University,Jinan.1-39(in Chinese)
Wu Z,Zhang M,Xie M,Dai Z,Wang X,Hu B,Ye H,Zeng X,2016.Extraction,characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali,HN1.Carbohydrate Polymers,137(3):541
Wu ZW,2014.Optimization fermentation,purification,structural elucidation and bioactivity of exopolysaccharides from Paecilomyces hepiali.PhD Dissertation,Nanjing Agricultural University,Nanjing.137-148(in Chinese)
Wu ZW,Lu JW,Wang XQ,Hu B,Ye H,Fan JL,Muhammad Abid,Zeng XX,2013.Optimization for production of exopolysaccharides with antitumor activity in vitro from Paecilomyces hepiali.Carbohydrate Polymers,99:226-234
Xiang M,Tang J,Zou XL,Zhao ZY,Wang YY,Xie SN,2009.βcell protecting and immunomodulatory activities of Paecilomyces hepiali Chen mycelium in STZ induced T1DM mice.The American Journal of Chinese Medicine,37(2):361-372
Xu CY,Li H,Lu ZM,2016.Analysis of the chemical component of Paecilomyces hepiali in submerged culture.Edible Fungi,38(3):64-66(in Chinese)
Xue Z,2017.The effect of“yi shen tong luo”recipe on rat testis SOD,MDA and serum T,FSH,LH of infertility model rats through adenine method.Master Thesis,Henan University of Chinese Medicine,Zhengzhou.1-28(in Chinese)
Yang D,2015.Study on of analgesic effective part of Paecilomyces hepiali and its sustained-release preparations.Master Thesis,Yunnan University of Traditional Chinese Medicine,Kunming.1-75(in Chinese)
Yu SJ,2012.Bioactivities and structure elucidation of exopolysaccharides from fermented broth of Paecilomyces hepiali.PhD Dissertation,Anhui Agricultural University,Hefei.1-65(in Chinese)
Zeng HW,Wang WF,Ma MH,Xu GH,Sun JF,Li WC,Yang YW,2016.Comparison of main bioactive composition and nutritional components among three fermented power commonly used as substitutes for Cordyceps sinensis.Journal of Yunnan Normal University,36(1):66-70(in Chinese)
Zhang XJ,Liu ZJ,Dang YH,Liu JW,Wu S,Wang YN,Li ZL,2013.Study on effects of apigenin on activity of testicular marker enzymes in mice.Journal of Medical Pest Control,29(11):1217-1221(in Chinese)
Zhang ZT,Xie GY,Chen K,Qi MY,Liu HR,2012.Alteration of marker enzymes in adenine-induced impaired testis and the interference of total flavonoids of herba epimedii in rats.Chongqing Medicine,41(7):683-685(in Chinese)
Zheng LF,2017.Study on pharmacodynamics and mechanism of“Jinkui Shenqi”pills in intervention of adenine-induced rat kidney“yang”deficiency model.Master Thesis,Beijing University of Chinese Medicine,Beijing.1-72(in Chinese)
戴如琴,沈崇尧,李晓明,兰江丽,林淑芳,邵爱娟,2017.蝙蝠蛾拟青霉模式菌相关问题的探讨.中国中药杂志,42(19):3843-3846
姜雪,王春月,李兰洲,王雪,滕利荣,周毓麟,2017.蝙蝠蛾拟青霉及蛹虫草与野生冬虫夏草活性比较.长春中医药大学学报,33(4):544-547
刘俊,杨本寿,姜国银,2012.蝙蝠蛾拟青霉的抑菌活性与菌丝体发酵化学组分的研究.昆明医科大学学报,33(6):32-34+38
王娟,2017.蝙蝠蛾拟青霉液体发酵菌丝体多糖对实验性糖尿病保护作用探讨.吉林大学博士论文,长春.1-103
王娜,2010.蝙蝠蛾拟青霉Cs-4对阿霉素肾病大鼠的肾脏保护作用.山东大学硕士论文,济南.1-39
武忠伟,2014.蝙蝠蛾拟青霉胞外多糖的发酵优化、结构鉴定及生物活性研究.南京农业大学博士论文,南京.137-148
何艳,程再兴,陈红,黄枚,2014.不同产地巴戟天盐制后补肾阳功效的比较研究.时珍国医国药,25(4):856-858
许春燕,李恒,陆震鸣,2016.蝙蝠蛾拟青霉发酵菌粉的主要成分分析.食用菌,38(3):64-66
薛铮,2017.益肾通络方对腺嘌呤法不育模型大鼠睾丸SOD、MDA和血清FSH、LH、T的影响.河南中医药大学硕士论文,郑州.1-28
阳丹,2015.蝙蝠蛾拟青霉菌丝体镇痛有效部位及其缓释制剂研究.云南中医学院硕士论文,昆明.1-75
于士军,2012.蝙蝠蛾拟青霉胞外多糖活性和结构的研究.安徽农业大学博士论文,合肥.1-65
曾化伟,王文风,马猛华,徐国华,孙见凡,厉文成,杨亚威,2016.三种替代冬虫夏草发酵菌粉的营养成分和活性成分的比较研究.云南师范大学学报(自然科学版),36(1):66-70
张晓晶,刘子婧,党瑜慧,刘佳微,吴双,汪燕妮,李芝兰,2013.芹菜素对小鼠睾丸标志酶活性的影响.医学动物防制,29(11):1217-1221
张作涛,谢高宇,陈凯,齐敏友,刘浩然,2012.腺嘌呤致大鼠睾丸损伤中标志酶的变化及淫羊藿总黄酮的干预作用.重庆医学,41(7):683-685
郑龙飞,2017.金匮肾气丸加味干预腺嘌呤所致大鼠肾阳虚模型的药效学及作用机理研究.北京中医药大学硕士论文,北京.1-72