林下山参冻干粉的急性毒性和遗传毒性研究
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摘要
目的研究林下山参冻干粉的急性毒性和遗传毒性。方法采用最大耐受剂量法进行小鼠急性毒性试验,通过Ames试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验检测林下山参冻干粉的遗传毒性。结果雌、雄小鼠对林下山参的最大耐受量(MTD)均>15.00 g/(kg·BW),属于无毒级物质;各剂量组的TA97、TA98、TA100、TA102菌株的回变菌落数与溶剂对照组比较,回变菌落数均未超过自发回变菌落数的1倍,亦无剂量-反应关系,结果为阴性;小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验中各剂量组微核率、精子畸形率与阴性对照组之间差异无统计学意义(P>0.05),而环磷酰胺阳性对照组与阴性对照组之间差异有统计学意义(P<0.01),显示试验结果均为阴性。结论在该试验条件下,林下山参冻干粉属于无毒级物质,亦不具有遗传毒性。
Objective To study the acute toxicity and genetic toxicity of freeze- dried powdered mountain cultivated ginseng.Methods Acute toxicity test for mice was done by test of maximum tolerated dose( MTD). Genetic toxicity was detected by ames test,micronucleus test of bone marrow PCE cell in mice and mice sperm abnormality test. Results The MTD of freeze-dried powdered mountain cultivated ginseng for mice were more than 15. 00 g /( kg·BW),it was a substance without toxicity.Comparing with the solvent control group,the reverse mutations of S. typhimurium TA97,TA98,TA100,TA102 were not one time more than nature mutations,and no dose- response relationship was found. Negative results appeared in ames test. Comparing with the negative control group,there were no statistical significance on the differences in micronucleus rate of micronucleus test of bone marrow PCE cell in mice and the teratospermia rate of mice sperm abnormality test( P > 0. 05),but there was statistical significance on the differences between the positive control groups of cyclophosphamide and negative control groups( P < 0. 01),demonstrating that test results were negative. Conclusion Under this experimental condition,freeze- dried powdered mountain is a non- toxic substance,and it has no genetic toxicity.
Objective To study the acute toxicity and genetic toxicity of freeze- dried powdered mountain cultivated ginseng.Methods Acute toxicity test for mice was done by test of maximum tolerated dose( MTD). Genetic toxicity was detected by ames test,micronucleus test of bone marrow PCE cell in mice and mice sperm abnormality test. Results The MTD of freeze-dried powdered mountain cultivated ginseng for mice were more than 15. 00 g /( kg·BW),it was a substance without toxicity.Comparing with the solvent control group,the reverse mutations of S. typhimurium TA97,TA98,TA100,TA102 were not one time more than nature mutations,and no dose- response relationship was found. Negative results appeared in ames test. Comparing with the negative control group,there were no statistical significance on the differences in micronucleus rate of micronucleus test of bone marrow PCE cell in mice and the teratospermia rate of mice sperm abnormality test( P > 0. 05),but there was statistical significance on the differences between the positive control groups of cyclophosphamide and negative control groups( P < 0. 01),demonstrating that test results were negative. Conclusion Under this experimental condition,freeze- dried powdered mountain is a non- toxic substance,and it has no genetic toxicity.
引文
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[3]金英花,刘洁,李龙云,等.人参皂苷Rg2冻干粉针对冠脉结扎犬心肌缺血的研究[J].中国中医急症,2015,24(6):1018:1021.
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[11]刘珊,冯晓莲,李晨汐,等.重组人乳铁蛋白的遗传毒性研究[J].癌变·畸变·突变,2011,23(5):384-387.
[12]Murthy HN,Dandin VS,Lee EJ,et al.Efficacy of ginseng adventitious root extract on hyperglycemia in streptozotocin-induceddiabeticrats[J].J Ethnopharmacol,2014,153(3):917-921.
[13]Seo H,Jeon BD,Ryu S.Persimmon vinegar ripening with the mountain-cultivated ginseng ingestion reduces blood lipids and lowers inflammatory cytokines in obese adolescents[J].J Exerc Nutr Biochem,2015,19(1):1-10.
[14]Niranjana Murthy H,Dandin VS,Yoeup Paek K,et al.Hepatoprotective activity of ginsenosides from Panax ginseng adventitious roots against carbon tetrachloride treated hepatic injury in rats[J].J Ethnopharmacol,2014,158:442-446.
[15]Lee K,Yu J,Sun S,et al.A 4-week,repeated,intravenous dose,toxicity test of mountain ginseng pharmacopuncture in sprague-dawley rats[J].2014,17(4):27-35.
[2]崔丽丽,闫梅霞,逄世峰,等.林下山参化学成分及质量评价研究进展[J].特产研究,2013(4):74-77.
[3]金英花,刘洁,李龙云,等.人参皂苷Rg2冻干粉针对冠脉结扎犬心肌缺血的研究[J].中国中医急症,2015,24(6):1018:1021.
[4]中华人民共和国卫生部.保健食品检验与评价技术规范[S].2003-02-04.
[5]李庆,杨颖,李欣,等.姜黄素的急性经口毒性和遗传毒性研究[J].中国卫生检验杂志,2011,21(7):1707-1709.
[6]李慧,李伟,王凤鸣.辅酶Q10软胶囊的急性毒性和遗传毒性研究[J].中国食物与营养,2010,16(11):66-69.
[7]李兴琴,张俊刚,洪丽华,等.β-胡萝卜素遗传毒性研究[J].职业健康,2012,28(5):550-552.
[8]李淑琴,李建国,李学敏,等.奇亚籽急性毒性及遗传毒性研究[J].癌变·畸变·突变,2013,25(6):470-473.
[9]姚文环,谢玮,杨非,等.富硒酵母的急性、亚急性和遗传毒性研究[J].中国卫生检验杂志,2012,22(11):2646-2648.
[10]孙兰,高峰,吴晓刚,等.人参粉剂安全性及遗传毒性研究[J].职业与健康,2011,27(22):2525-2527.
[11]刘珊,冯晓莲,李晨汐,等.重组人乳铁蛋白的遗传毒性研究[J].癌变·畸变·突变,2011,23(5):384-387.
[12]Murthy HN,Dandin VS,Lee EJ,et al.Efficacy of ginseng adventitious root extract on hyperglycemia in streptozotocin-induceddiabeticrats[J].J Ethnopharmacol,2014,153(3):917-921.
[13]Seo H,Jeon BD,Ryu S.Persimmon vinegar ripening with the mountain-cultivated ginseng ingestion reduces blood lipids and lowers inflammatory cytokines in obese adolescents[J].J Exerc Nutr Biochem,2015,19(1):1-10.
[14]Niranjana Murthy H,Dandin VS,Yoeup Paek K,et al.Hepatoprotective activity of ginsenosides from Panax ginseng adventitious roots against carbon tetrachloride treated hepatic injury in rats[J].J Ethnopharmacol,2014,158:442-446.
[15]Lee K,Yu J,Sun S,et al.A 4-week,repeated,intravenous dose,toxicity test of mountain ginseng pharmacopuncture in sprague-dawley rats[J].2014,17(4):27-35.