人参全草免疫调节作用研究
|
摘要
目的:以林下山参为例,探究人参全草对小鼠免疫功能的影响。方法:选用昆明种小鼠,进行刀豆蛋白A(Con A)诱导的小鼠脾淋巴细胞转化实验、二硝基氟苯(DNFB)诱导小鼠迟发型超敏反应(DTH)实验、抗体生成细胞检测、血清溶血素测定、小鼠碳廓清实验、小鼠腹腔巨噬细胞吞噬鸡红细胞实验、自然杀伤细胞(NK细胞)活性测定,探讨林下山参全草是否具有调节免疫力功能的作用。结果:林下山参全草对实验动物胸腺指数、脾脏指数无明显影响。各实验剂量组的林下山参全草对DNFB诱导的小鼠DTH的形成无明显促进作用,中、高剂量组的林下山参全草对Con A诱导的小鼠脾淋巴细胞增殖能力显著增强(P <0. 01),低、中剂量组的林下山参全草对小鼠抗体生成细胞数量、血清溶血素抗体积数均有显著提高作用(P <0. 01),低剂量组的林下山参全草能明显增强小鼠碳廓清能力(P <0. 01),各剂量组的林下山参全草对小鼠腹腔巨噬细胞吞噬鸡红细胞的能力、NK细胞活性均未表现出明显提高作用。结论:林下山参全草具有一定的调节小鼠免疫的作用。
Objective: To investigate the immune enhancement effect of the whole plant of ginseng in mice,take Lin-Xia-Shan Shen as an example. Methods: The spleen lymphocyte transformation experiment induced by Con A, the delayed type hypersensitivity( DTH) reaction induced by DNFB,the dectection of antibody-producing,and the determination of serum hemolysin experiments were carried out with the Kunming mice. Furthermore,the carbon clearance test,the phagocytosis effect of macrophages with chicken red blood cell experiments,and the determination of natural killer cell activity were also carried out in order to explore the immunoregulation effect of Lin-Xia-Shan-Shen. Results: There was no significant effect on the thymus index and spleen index of the experimental animals treated with Lin-Xia-Shan-Shen. The promotion effect on DTH reaction induced by DNFB experimental dose of mountain cultivated ginseng whole plant had no significant effect on the f were also not observed. The mice treated with middle and high doses of Lin-Xia-Shan-Shen possessed potent proliferation effect on the spleen lymphocyte in mice induced by Con A( P < 0. 01). low and medium doses of mountain cultivated ginseng whole plant significantly increased The number cells of antibody and the accumulation value of anti-body in serum was increased significantly in the groups treated with low and middle dose of Lin-Xia-Shan-Shen( P < 0. 01). Furthermore,the clearance ability of carbon particle was enhanced significantly in the low dose group( P < 0. 01). However,the phagocytosis ability of macrophages,and the activity of NK cells were not increased obviously in all groups. Conclusion: The whole plant of Lin-Xia-Shan-Shen has a certain role in adjust immunity in mice.
Objective: To investigate the immune enhancement effect of the whole plant of ginseng in mice,take Lin-Xia-Shan Shen as an example. Methods: The spleen lymphocyte transformation experiment induced by Con A, the delayed type hypersensitivity( DTH) reaction induced by DNFB,the dectection of antibody-producing,and the determination of serum hemolysin experiments were carried out with the Kunming mice. Furthermore,the carbon clearance test,the phagocytosis effect of macrophages with chicken red blood cell experiments,and the determination of natural killer cell activity were also carried out in order to explore the immunoregulation effect of Lin-Xia-Shan-Shen. Results: There was no significant effect on the thymus index and spleen index of the experimental animals treated with Lin-Xia-Shan-Shen. The promotion effect on DTH reaction induced by DNFB experimental dose of mountain cultivated ginseng whole plant had no significant effect on the f were also not observed. The mice treated with middle and high doses of Lin-Xia-Shan-Shen possessed potent proliferation effect on the spleen lymphocyte in mice induced by Con A( P < 0. 01). low and medium doses of mountain cultivated ginseng whole plant significantly increased The number cells of antibody and the accumulation value of anti-body in serum was increased significantly in the groups treated with low and middle dose of Lin-Xia-Shan-Shen( P < 0. 01). Furthermore,the clearance ability of carbon particle was enhanced significantly in the low dose group( P < 0. 01). However,the phagocytosis ability of macrophages,and the activity of NK cells were not increased obviously in all groups. Conclusion: The whole plant of Lin-Xia-Shan-Shen has a certain role in adjust immunity in mice.
引文
[1] ZHANG G,LIU A,ZHOU Y,et al. Panax ginseng ginsenoside-Rg2 protects memory impairment via antiapoptosis in a rat model with vascular dementia[J]. J Ethnopharmacol,2008,115(3):441-448.
[2] YU X T,WANG S P. Clinical ob servation on treatment for postoperative gastric cancer by ginsenoside Rg3combined with chemotherapy[J]. Chin J Cancer Prev Treat,2010,17(10):779-781.
[3] ZHANG Z,JIANG B,ZHENG X,et al. Immune enhancing effects of Rg3 on peripheral lymphocytes in vitro in cancer patients treated with radiotherapy[J]. Chin Pharm J,2004,39(4):261-264.
[4] SUI D Y,YU X F,QU S C,et al. Effects of Panax quinquefolium 20S-2protopanaxdiol saponins on experimental ventricular remodeling in rat[J]. Chin Pharm J,2007,42(2):108-112.
[5] LEE Y,JIN Y,LIM W,et al. A ginsenoside-Rh1,a component of ginseng saponin,activates estrogen receptor in human breast carcinoma MCF-7 cells[J]. J Steroid Biochem,2003,84(4):463-468.
[6] WILLIAM C S,CHUNG W S,SALLY K W,et al. Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats[J]. Eur J Pharmacol,2006,550(1/3):173-179.
[7] KIM S E,LEE Y H,PARK H J,et al. Ginsenoside-Rs4,a new type of ginseng saponin concurrently induces apoptosis and selectively elevates protein levels of p53 and p21 WAF1in human hepatoma SK-HEP-1 cells European[J]. Eur J Cancer,1999,35(3):507-511.
[8]张自广.中药复方多糖的优化提取及其对小鼠免疫功能的影响[D].合肥:安徽农业大学,2011.
[9]曾祥云,仇仁春,曹志强.辽东本草牌灵片增强免疫力功能实验研究[J].人参研究,2017,29(3):14-18.
[10]曹发昊.复方人参皂苷纳米乳的制备及其免疫增强作用的研究[D].咸阳:西北农林科技大学,2012.
[11]王景尚,黄烨,殷惠军.从全人参入药谈气的生成[J].中华中医药杂志,2014,29(10):3050-3052.
[12]付秀花.中草药组方对小鼠免疫功能的影响[D].南京:南京农业大学,2003.
[13] JERRELLS T R,MALLAVIA L P,HINRICHS D J. Detection of long-term cellular immunity to Coxiella burneti as assayed by lymphocyte transformation.[J]. Infection&Immunity,1975,11(2):280.
[14]韩玉生,徐颖,周忠光,等.强脊康对小鼠体液免疫功能影响的实验研究[J].中医药信息,2007,24(4):64-65.
[15]马得莹,单安山.中草药对动物生长、内分泌和免疫功能的影响[J].中国畜牧兽医,2004,31(1):16-19.
[16] MILAN M,DIAZ BENJUMEA F J,COHEN S M. Beadex encodes an LMO protein that regulates Apterous LIM-homeodomain activity in Drosophila wing development:amodel for LMO oncogene function[J]. Genes Dev. 1998,12(18):2912-2920.
[17]周娅红.人参总皂苷对免疫低下小鼠免疫功能的影响[J].中国中医急症,2010,19(9):1559-1561.
[18]赵萧萧.人参叶多糖提取及其免疫作用的研究[D].大连:大连工业大学,2009.
[19]曾祥云,曹志强,仇仁春.辽东本草牌人参片增强免疫力功能实验研究[J].人参研究,2015,28(4):25-30.
[20] CHO J Y,KIM A R,YOO E S,et al. Ginsenosides from Panax ginseng differentially regulate lymphocyte proliferation.[J].Planta Medica,2002,68(06):497-500.
[21] WANG Y,WANG B X,LIU T H,et al. Metabolism of ginsenoside Rg1 by intestinal bacteria. II. Immunological activity of ginsenoside Rg1 and Rh1[J]. Acta Pharmacologica Sinica,2000,21(9):792-796.
[22]杨秀伟,富力.人参中三萜类化学成分的生物学活性和药理学作用[J].中国现代中药,2016,18(1):36-55.
[23]丁艳芬,李江霞,杨崇仁.人参皂苷Rh1的药理作用研究进展[J].中国现代中药,2013,15(4):282-285.
[2] YU X T,WANG S P. Clinical ob servation on treatment for postoperative gastric cancer by ginsenoside Rg3combined with chemotherapy[J]. Chin J Cancer Prev Treat,2010,17(10):779-781.
[3] ZHANG Z,JIANG B,ZHENG X,et al. Immune enhancing effects of Rg3 on peripheral lymphocytes in vitro in cancer patients treated with radiotherapy[J]. Chin Pharm J,2004,39(4):261-264.
[4] SUI D Y,YU X F,QU S C,et al. Effects of Panax quinquefolium 20S-2protopanaxdiol saponins on experimental ventricular remodeling in rat[J]. Chin Pharm J,2007,42(2):108-112.
[5] LEE Y,JIN Y,LIM W,et al. A ginsenoside-Rh1,a component of ginseng saponin,activates estrogen receptor in human breast carcinoma MCF-7 cells[J]. J Steroid Biochem,2003,84(4):463-468.
[6] WILLIAM C S,CHUNG W S,SALLY K W,et al. Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats[J]. Eur J Pharmacol,2006,550(1/3):173-179.
[7] KIM S E,LEE Y H,PARK H J,et al. Ginsenoside-Rs4,a new type of ginseng saponin concurrently induces apoptosis and selectively elevates protein levels of p53 and p21 WAF1in human hepatoma SK-HEP-1 cells European[J]. Eur J Cancer,1999,35(3):507-511.
[8]张自广.中药复方多糖的优化提取及其对小鼠免疫功能的影响[D].合肥:安徽农业大学,2011.
[9]曾祥云,仇仁春,曹志强.辽东本草牌灵片增强免疫力功能实验研究[J].人参研究,2017,29(3):14-18.
[10]曹发昊.复方人参皂苷纳米乳的制备及其免疫增强作用的研究[D].咸阳:西北农林科技大学,2012.
[11]王景尚,黄烨,殷惠军.从全人参入药谈气的生成[J].中华中医药杂志,2014,29(10):3050-3052.
[12]付秀花.中草药组方对小鼠免疫功能的影响[D].南京:南京农业大学,2003.
[13] JERRELLS T R,MALLAVIA L P,HINRICHS D J. Detection of long-term cellular immunity to Coxiella burneti as assayed by lymphocyte transformation.[J]. Infection&Immunity,1975,11(2):280.
[14]韩玉生,徐颖,周忠光,等.强脊康对小鼠体液免疫功能影响的实验研究[J].中医药信息,2007,24(4):64-65.
[15]马得莹,单安山.中草药对动物生长、内分泌和免疫功能的影响[J].中国畜牧兽医,2004,31(1):16-19.
[16] MILAN M,DIAZ BENJUMEA F J,COHEN S M. Beadex encodes an LMO protein that regulates Apterous LIM-homeodomain activity in Drosophila wing development:amodel for LMO oncogene function[J]. Genes Dev. 1998,12(18):2912-2920.
[17]周娅红.人参总皂苷对免疫低下小鼠免疫功能的影响[J].中国中医急症,2010,19(9):1559-1561.
[18]赵萧萧.人参叶多糖提取及其免疫作用的研究[D].大连:大连工业大学,2009.
[19]曾祥云,曹志强,仇仁春.辽东本草牌人参片增强免疫力功能实验研究[J].人参研究,2015,28(4):25-30.
[20] CHO J Y,KIM A R,YOO E S,et al. Ginsenosides from Panax ginseng differentially regulate lymphocyte proliferation.[J].Planta Medica,2002,68(06):497-500.
[21] WANG Y,WANG B X,LIU T H,et al. Metabolism of ginsenoside Rg1 by intestinal bacteria. II. Immunological activity of ginsenoside Rg1 and Rh1[J]. Acta Pharmacologica Sinica,2000,21(9):792-796.
[22]杨秀伟,富力.人参中三萜类化学成分的生物学活性和药理学作用[J].中国现代中药,2016,18(1):36-55.
[23]丁艳芬,李江霞,杨崇仁.人参皂苷Rh1的药理作用研究进展[J].中国现代中药,2013,15(4):282-285.