促生长激素神经肽对坐骨神经损伤后血管内皮生长因子表达的影响
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摘要
目的研究促生长激素神经肽(Gal)对大鼠坐骨神经损伤后血管内皮生长因子(VEGF)表达的影响。方法共选取36只健康雄性SD大鼠,将其随机分为正常组(A组)、对照组(B组)、促生长激素神经肽组(C组),每组大鼠12只。A组不做任何处理,B、C组造成大鼠坐骨神经SeddonⅡ度损伤模型,24 h后进行实验,A组正常饮食、不进行任何治疗,B组加强营养、不进行任何治疗,C组进行注射Gal 3μg/d治疗,每天1次,共4周。分别于治疗2周和4周后,采用免疫组化染色方法,检测大鼠血管内皮生长因子(VEGF)的表达。结果 2周时,B、C组VEGF达到最高值,4周时开始下降,且B、C组两两比较,VEGF在2周和4周时均有增高。结论促生长激素神经肽可以使坐骨神经损伤后修复加快,同时坐骨神经损伤后的修复机制可能与血管内皮生长因子(VEGF)的表达有关。
Objective To Study the galanin on vascular injury endothelial growth factor(VEGF) expression effects after rat sciatic nerve.Methods A total of thirty-six healthy male SD rats, were randomly divided into normal group(A group), control group(B group), galanin group(C group), 12 rats in each group. A group without any treatment, B and C group of rats were caused by sciatic nerve injury model of Seddon Ⅱ. Rats began experimenting after 24 hours, A group was fed with normal diet without any treatment, B group strengthened nutrition without any treatment, C group was injected with galanin 3 μg/d. Therapy time was a time a day, for 4 weeks. After 2 weeks and 4 weeks, we used rat footprinting method, immunohistochemical method to understand the rat sciatic nerve function index(SFI) score, and detect rat vascular endothelial growth factor(VEGF)expression. Results At 2 weeks, VEGF of B, C group reached the highest value, and began to decline at 4 weeks. B, C group VEGF at 2 weeks and4 weeks has increased respectively. Conclusion Galanin can accelerate repair of sciatic nerve injury, and repair mechanism after sciatic nerve injury may be related to vascular endothelial growth factor(VEGF) expression.
Objective To Study the galanin on vascular injury endothelial growth factor(VEGF) expression effects after rat sciatic nerve.Methods A total of thirty-six healthy male SD rats, were randomly divided into normal group(A group), control group(B group), galanin group(C group), 12 rats in each group. A group without any treatment, B and C group of rats were caused by sciatic nerve injury model of Seddon Ⅱ. Rats began experimenting after 24 hours, A group was fed with normal diet without any treatment, B group strengthened nutrition without any treatment, C group was injected with galanin 3 μg/d. Therapy time was a time a day, for 4 weeks. After 2 weeks and 4 weeks, we used rat footprinting method, immunohistochemical method to understand the rat sciatic nerve function index(SFI) score, and detect rat vascular endothelial growth factor(VEGF)expression. Results At 2 weeks, VEGF of B, C group reached the highest value, and began to decline at 4 weeks. B, C group VEGF at 2 weeks and4 weeks has increased respectively. Conclusion Galanin can accelerate repair of sciatic nerve injury, and repair mechanism after sciatic nerve injury may be related to vascular endothelial growth factor(VEGF) expression.
引文
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[11] Chen F, Tan Z, Donq C, et al. Combination of VEGF(165)Angiopoietin-l Geneand 14 endothelial progenitor cells for therapeutic neovaseularization Eur[J].Pharmacol, 2007,5(10):95-99.
[12] BW Park, JR Kim, J-H Lee, et a1. Expressionof nerve growth factor and vascular endothelial growth factor in the inferior alveolar nerve after distraction osteogenesis[J]. OralMaxillofac, 2006, 2(35):624-630.
[13] Chavez JC, Almhanna K, Berti-Mattera LN. Transientexpression of hypoxia-Inducible factor-1 alpha and target genesin peripheral nerves from diabetic rats[J].NeurosciLett, 2005,374(4):179-182.
[14] Tang K, Breen EC, Waqner H, et a1. HIF and VEGF relationships inresponse to hypoxia and sciatic nerve stimulation in rat gastrocnemius[J]. Respir Physiol Neurobiol, 2004,144(1):71-80.
[15] Towler DA. Vascular biology and bone formation:hints from HIT[J]. ClinInvest, 2007,117(6):1477-1480.
[16] Jianq M, Wanq B, Wang C, et al. Angiogenesis by transplantation of HIT-l hamodified EPCs into ischemic limbs[J]. CellBiochem, 2007,5(31):89-96.
[2] Sherman D, Halamish-Shani T, Gershtansky Y, et al. Analysis of brachial plexus injuries reported to MRM[J].Harefuah, 2010,149(2):71-76,125-126.
[3] Mikami Y, Oqura T, Kubo T, et al. Inducing peripheral sympathetic nerve activity by therapeutic electrical stimulation[J]. Orthop Surg(Hong Kong), 2009,13(2):167-170.
[4] Elena A, Esther U, Jessica JM, et a1. Electrical stimulation combined with exercise increase axonal regeneration after peripheral nerve injury[J]. Exp Neurol, 2009,219:258-265.
[5] Tang J, Hua Y, Su J, et al. Expression of VEGF and neural repair after alprostadil treatment in a rat model of sciatic nerve crush injury[J]. Neurol india, 2009,57(4):387-394.
[6]张素娟,吴军.微波对坐骨神经损伤大鼠脊髓内生长相关蛋白-43表达的影响[J].中国康复医学杂志,2011,26(11):1004-1008.
[7] Thil MA, Vince V, Veraart C, et a1. Nitric oxidesynthases II and III and vascular endothelial growth factor areup-regulatedin sciatic nerve after spiralcuff electrode implantation[J]. Neuroimmunol, 2005,166(2):158-166.
[8] Scarlato M, Previtali SC, Carpo M, et a1. Polyneuropathy in POEMS syndrome:roleof angiogenic factors in thepathogenesis[J]. Brain, 2005,128(8):1911-1920.
[9] Kurvliszvn-Moskal A, Klimiuk PA, Sierakowski S, et al. Vascular endothelial growth factorin systemic lupus erythematosus Relationship to diseaseactivity, systemic organ manifestation, and nailfold capillaroscopic abnormalities[J]. Arch Immunol TilerExp(warsz), 2007,1(8):22-26.
[10] Kosmidou I, Karmpaliotis D, Kirtalle AJ, et a1. Vascular endothelial growth factors in pulmonaryedema:allupdate[J]. Thromb Thrombolysis, 2007,2(7):37-42.
[11] Chen F, Tan Z, Donq C, et al. Combination of VEGF(165)Angiopoietin-l Geneand 14 endothelial progenitor cells for therapeutic neovaseularization Eur[J].Pharmacol, 2007,5(10):95-99.
[12] BW Park, JR Kim, J-H Lee, et a1. Expressionof nerve growth factor and vascular endothelial growth factor in the inferior alveolar nerve after distraction osteogenesis[J]. OralMaxillofac, 2006, 2(35):624-630.
[13] Chavez JC, Almhanna K, Berti-Mattera LN. Transientexpression of hypoxia-Inducible factor-1 alpha and target genesin peripheral nerves from diabetic rats[J].NeurosciLett, 2005,374(4):179-182.
[14] Tang K, Breen EC, Waqner H, et a1. HIF and VEGF relationships inresponse to hypoxia and sciatic nerve stimulation in rat gastrocnemius[J]. Respir Physiol Neurobiol, 2004,144(1):71-80.
[15] Towler DA. Vascular biology and bone formation:hints from HIT[J]. ClinInvest, 2007,117(6):1477-1480.
[16] Jianq M, Wanq B, Wang C, et al. Angiogenesis by transplantation of HIT-l hamodified EPCs into ischemic limbs[J]. CellBiochem, 2007,5(31):89-96.