反相高效液相色谱法测定曲咪新乳膏中醋酸曲安奈德和硝酸咪康唑的含量
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摘要
目的探讨测定曲咪新乳膏中醋酸曲安奈德和硝酸咪康唑含量的方法。方法采用反相高效液相色谱法。色谱柱为Agilent SB-C_(18)(150 mm×4.6 mm,5.0μm);以A甲醇∶0.05%乙酸铵(85∶15),B甲醇∶0.05%乙酸铵(75∶25)为流动相,采用梯度洗脱;流速1.0 mL/min;柱温30℃;检测波长240 nm,进样量:20μL。结果醋酸曲安奈德在0.01～0.06 mg/mL范围内线性关系良好(r=1.000 0,n=5),平均回收率为100.52%,RSD=0.62%;硝酸咪康唑在0.1～0.6 mg/mL范围内线性关系良好(r=0.999 6,n=5),平均回收率为99.93%,RSD=0.86%。结论此方法可同时测定曲咪新乳膏中两组分的含量,方法简便,准确,快速。
Objective To establish a RP-HPLC method to determinate the contents of triamcinolone acetonide acetate and miconazole nitrate in triamcinolone acetonide and miconazole nitrate and neomycin sulfate cream.Methods Using Agilent SB-C_(18)(150 mm×4.6 mm,5.0 μm)column;Mobile phase A was methyl alcohol and 0.05% ammonium acetate in(85∶15),Mobile phase B was methyl alcohol and 0.05% ammonium acetate in(75∶25),gradient elution;Velocity:1.0 mL/min;The column temperature was 30 ℃.The UV detection wavelength was 240 nm and the inject volume was 20 μL.Result The linear hypotaxis of triamcinolone acetonide was good in the scope of 0.01-0.06 mg/mL(r=1.000 0,n=5).The average recycle ratio was 100.52%(RSD=0.62%).The linear hypotaxis of Miconazole Nitrate was good in the scope of 0.1-0.6 mg/mL(r=0.999 6,n=5).The average recycle ratio was 99.93%(RSD=0.86%).Conclusion This method can simultaneously determine the contents of the two components in triamcinolone acetonide and miconazole nitrate and neomycin sulfate cream.The method is simple,accurate and fast.
Objective To establish a RP-HPLC method to determinate the contents of triamcinolone acetonide acetate and miconazole nitrate in triamcinolone acetonide and miconazole nitrate and neomycin sulfate cream.Methods Using Agilent SB-C_(18)(150 mm×4.6 mm,5.0 μm)column;Mobile phase A was methyl alcohol and 0.05% ammonium acetate in(85∶15),Mobile phase B was methyl alcohol and 0.05% ammonium acetate in(75∶25),gradient elution;Velocity:1.0 mL/min;The column temperature was 30 ℃.The UV detection wavelength was 240 nm and the inject volume was 20 μL.Result The linear hypotaxis of triamcinolone acetonide was good in the scope of 0.01-0.06 mg/mL(r=1.000 0,n=5).The average recycle ratio was 100.52%(RSD=0.62%).The linear hypotaxis of Miconazole Nitrate was good in the scope of 0.1-0.6 mg/mL(r=0.999 6,n=5).The average recycle ratio was 99.93%(RSD=0.86%).Conclusion This method can simultaneously determine the contents of the two components in triamcinolone acetonide and miconazole nitrate and neomycin sulfate cream.The method is simple,accurate and fast.
引文
[1] 国家药典委员会.国家药品标准-化学药品地方标准上升国家标准第2册[S].2002:67-68.
[2] 刘文英.药物分析[M].5版.北京:人民卫生出版社,2003:258.
[3] 刘光斌,刘效栓,姜芳宁,等.高效液相色谱法测定湿疹乳膏中氯霉素和醋酸曲安奈德含量[J].中国药业,2010,19(23):34.
[4] 吴月萍.高效液相法测定硝酸咪康唑乳膏含量[J].海峡药学,2013,25(10):81-83.
[5] 杨瑾,莫国栋,曾维国,等.高效液相色谱法测定复方咪康唑洗剂中硝酸咪康唑含量[J].中国药业,2016,25(7):46-48.
[6] 冯春景,刘皈阳,马建丽,等.HPLC法同时测定复方醋酸曲安奈德搽剂中盐酸普鲁卡因和醋酸曲安奈德的含量[J].解放军药学学报,2015,31(5):417-419.
[7] 黄兴汉,费路华,陈春游.HPLC法测定复方醋酸曲安奈德乳膏的含量[J].中国药师,2011,14 (1):90.
[8] 李蔚,马嘉,吴静,等.HPLC法测定糠酸莫米松乳膏含量[J].安徽医药,2014,18(10):1854-1856.
[9 ] 李杰,欧余航,陈强,等.复方九节茶乳膏的质量标准研究[J].安徽医药,2015,19(10):1869-1872.
[10] 国家药典委员会.中国药典(二部)[S].北京:中国医药科技出版社,2015:1533.
[2] 刘文英.药物分析[M].5版.北京:人民卫生出版社,2003:258.
[3] 刘光斌,刘效栓,姜芳宁,等.高效液相色谱法测定湿疹乳膏中氯霉素和醋酸曲安奈德含量[J].中国药业,2010,19(23):34.
[4] 吴月萍.高效液相法测定硝酸咪康唑乳膏含量[J].海峡药学,2013,25(10):81-83.
[5] 杨瑾,莫国栋,曾维国,等.高效液相色谱法测定复方咪康唑洗剂中硝酸咪康唑含量[J].中国药业,2016,25(7):46-48.
[6] 冯春景,刘皈阳,马建丽,等.HPLC法同时测定复方醋酸曲安奈德搽剂中盐酸普鲁卡因和醋酸曲安奈德的含量[J].解放军药学学报,2015,31(5):417-419.
[7] 黄兴汉,费路华,陈春游.HPLC法测定复方醋酸曲安奈德乳膏的含量[J].中国药师,2011,14 (1):90.
[8] 李蔚,马嘉,吴静,等.HPLC法测定糠酸莫米松乳膏含量[J].安徽医药,2014,18(10):1854-1856.
[9 ] 李杰,欧余航,陈强,等.复方九节茶乳膏的质量标准研究[J].安徽医药,2015,19(10):1869-1872.
[10] 国家药典委员会.中国药典(二部)[S].北京:中国医药科技出版社,2015:1533.