屠宰废水中COD降解菌分离鉴定与特性研究
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摘要
通过选择性富集培养、驯化培养、划线分离和纯化等方法,从湖南某肉制品有限公司的屠宰废水中筛选到4株COD降解菌株,编号为1～4号。并对4株COD降解菌进行驯化与初步分离,采用高锰酸钾法测定各菌株的COD降解率,其中3号菌株的COD降解率最高,达到11.30%。经过观察3号菌株的形态特征,以及革兰氏染色实验,初步鉴定3号菌株为盐水球菌属细菌。并对其培养温度、pH值、蛋白胨浓度和酵母膏浓度等特性进行了研究,单因子优化实验结果表明,3号菌的最适生长温度为37℃、最适pH值为6、培养基中蛋白胨的质量分数为1.5%、酵母膏的质量分数为0.8%。
By means of selective enrichment cultivation,domestication,streaking cultivation and purification,four COD degrading strains,numbered 1,2,3 and 4 respectively,have been isolated and separated from slaughterhouse wastewater in a meat product Co.,Ltd.,Hunan Province.A preliminary separation has been made of the four domesticated strains of COD-degrading bacteria,and the potassium permanganate method has been adopted to determine the COD degradation rate of strains,with three of them reaching the highest rate of 11.30%.After observing the morphological characteristics of No.3 strain and Gram staining test,it has been preliminarily identified that No.3 strain proves to be Salinococcus.A research has been conducted on the characteristics of culture temperature,pH value,peptone concentration and yeast extract concentration,with the results of single factor optimization showing that the optimum growth temperature of No.3 strain will be 37 ℃,with the optimum pH value 6,the concentration of peptone in culture medium 1.5%,and the yeast extract concentration 0.8%.
By means of selective enrichment cultivation,domestication,streaking cultivation and purification,four COD degrading strains,numbered 1,2,3 and 4 respectively,have been isolated and separated from slaughterhouse wastewater in a meat product Co.,Ltd.,Hunan Province.A preliminary separation has been made of the four domesticated strains of COD-degrading bacteria,and the potassium permanganate method has been adopted to determine the COD degradation rate of strains,with three of them reaching the highest rate of 11.30%.After observing the morphological characteristics of No.3 strain and Gram staining test,it has been preliminarily identified that No.3 strain proves to be Salinococcus.A research has been conducted on the characteristics of culture temperature,pH value,peptone concentration and yeast extract concentration,with the results of single factor optimization showing that the optimum growth temperature of No.3 strain will be 37 ℃,with the optimum pH value 6,the concentration of peptone in culture medium 1.5%,and the yeast extract concentration 0.8%.
引文
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[19]张立阳,张幸怡,张薇,等.产几丁质酶菌株的筛选、鉴定及其对玉米秸秆中优势霉菌的抑制作用[J].动物营养学报,2017,29(3):970-978.ZHANG Liyang,ZHANG Xingyi,ZHANG Wei,et al.Screening and Identification of Chitinase Producing Strain and Its Inhibition Action to Dominant Moulds in Corn Stalk[J].Chinese Journal of Animal Nutrition,2017,29(3):970-978.
[20]李丹,严红光,袁亮.蓝莓果酒专用酵母的筛选、鉴定与性能研究[J].食品与发酵科技,2015,51(5):75-79.LI Dan,YAN Hongguang,YUAN Liang.Screening,Identification and Study of the Fermentation Properties of Yeast Strains for Exclusive Use of Blueberry Wine[J].Food and Fermentation Technology,2015,51(5):75-79.
[21]谭才邓,王文文,朱美娟,等.1株耐高温高盐生香酵母的选育及特性分析[J].食品与发酵工业,2016,42(3):92-96.TAN Caideng,WANG Wenwen,ZHU Meijuan,et al.Screening and Characterization of Aroma Yeast with Thermo-Tolerant and Salt-Tolerance[J].Food and Fermentation Industries,2016,42(3):92-96.
[2]林庆华.家禽屠宰废水处理初探[J].中国环保产业,2016(8):42-45.LIN Qinghua.Probe into Wastewater Treatment of Poultry Slaughter[J].China Environmental Protection Industry,2016(8):42-45.
[3]金玉,任滨侨,赵路阳,等.屠宰废水处理研究进展[J].黑龙江科学,2015(6):38-39.JIN Yu,REN Binqiao,ZHAO Luyang,et al.Research Progress in Slaughterhouse Wasterwater Treatment[J].Heilongjiang Science,2015(6):38-39.
[4]汤敏.焦化废水物化处理技术研究进展[J].广东化学,2016,43(6):129-131.TANG Min.Research Progress of the Coking Wastewater Treatment by Physical Processes[J].Guangdong Chemical Industry,2016,43(6):129-131.
[5]王晓星,黄应平,邹雪,等.草甘膦废水有机污染物的微生物降解[J].环境科学与技术,2010,33(8):28-32.WANG Xiaoxing,HUANG Yingping,ZOU Xue,et al.Biodegradation of Organic Pollutants in Glyphosate Wastewater[J].Environmental Science&Technology,2010,33(8):28-32.
[6]郭燕妮,方增坤,胡杰华,等.化学沉淀法处理含重金属废水的研究进展[J].工业水处理,2011,31(12):9-13.GUO Yanni,FANG Zengkun,HU Jiehua,et al.Research Development of Treating Wastewater Containing Heavy Metals by Chemical Precipitation Process[J].Industrial Water Treatment,2011,31(12):9-13.
[7]谢小兵.两级兼氧+好氧处理技术在屠宰废水处理中的应用研究[D].南昌:南昌大学,2013.Xie Xiaobing.Application and Analysis of the Technology for Treament of Slaughterhous Wastewater[D].Nanchang:Nanchang University,2013.
[8]KUNDU P,DEBSARKAR A,MUKHERJEE S,et al.Artificial Neural Network Modelling in Biological Removal of Organic Carbon and Nitrogen for the Treatment of Slaughterhouse Wastewater in a Batch Reactor[J].Environmental Technology,2014,35(9/10/11/12):1296-1306.
[9]蔡明明,张培玉,李阳,等.北极生活污水低温降解菌筛选与多样性分析[J].环境科学与技术,2016,39(6):7-12.CAI Mingming,ZHANG Peiyu,LI Yang,et al.Screening and Phylogenetic Analysis of SewageDegrading Microorganism in Arctic Marine Sediments[J].Environmental Science&Technology,2016,39(6):7-12.
[10]贾艳萍,贾心倩,宗庆,等.生物技术在屠宰废水处理中的应用研究进展[J].工业水处理,2015,35(3):10-12.JIA Yanping,JIA Xinqian,ZONG Qing,et al.Research Progress in the Application of Biological Technology to Slaughterhouse Wastewater Treatment[J].Industrial Water Treatment,2015,35(3):10-12.
[11]张玉秀,柴团耀.废水生物处理过程中污泥的微生物种群结构和PAHs降解菌研究进展[J].中国科学院大学学报,2016,33(1):1-8.ZHANG Yuxiu,CHAI Tuanyao.Microbial Community Structure and PAHs-Degrading Bacteria of Activated Sludge in Biological Wastewater Treatment Process[J].Journal of University of Chinese Academy of Sciences,2016,33(1):1-8.
[12]宋永庆,张龙,李南华,等.絮凝菌的筛选、培养条件优化及对屠宰场废水的处理[J].安全与环境学报,2016,16(3):211-215.SONG Yongqing,ZHANG Long,LI Nanhua,et al.Screening the Flocculent Bacteria and Optimizing the Fermentation Conditions in Treating Slaughter-Releasing Sewage[J].Journal of Safety and Environment,2016,16(3):211-215.
[13]沈万峰.有机废水的好氧生物处理技术进展研究[J].城市道桥与防洪,2017(9):105-106.SHEN Wanfeng.Study on Aerobic Biological Treatment Technology of Organic Wastewater[J].Urban Roads Bridges&Flood Control,2017(9):105-106.
[14]苏凤贤,张井,郑晓杰.人参果自然发酵醪中酵母菌种筛选及其发酵性能[J].食品科学,2017,38(4):100-106.SU Fengxian,ZHANG Jing,ZHENG Xiaojie,et al.Screening and Fermentation Characteristics of Indigenous Yeast Strains From Spontaneously Fermented Pepino Fruit(Solanum Muricatum Ait.)[J].Food Science,2017,38(4):100-106.
[15]陆薇帏,汪立平.纯化功能性大豆低聚糖酵母菌的筛选及发酵特性初步应用[J].食品与发酵工业,2016,42(3):168-171.LU Weiwei,WANG Liping.Isolation and Identification of Yeasts for Purification of Soy Oligosaccharides and Preliminary Application of Its Fermentation Characteristics[J].Food and Fermentation Industries.2016,42(3):168-171.
[16]DUAN J,HUO X,DU W J,et al.Biodegradation of Kraft Lignin by a Newly Isolated Anaerobic Bacterial Strain,Acetoanaerobium sp.WJDL-Y2[J].Letters in Applied Microbiology,2016,62(1):55-62.
[17]张为艳,刘鹏程,郑凤娟,等.有机化工废水COD高效降解菌的分离筛选及应用[J].工业水处理,2016,36(8):52-54ZHANG Weiyan,LIU Pengcheng,ZHENG Fengjuan,et al.Separation,Screening and Application of Efficient Degradation Microorganisms for Improving CODRemoving Rates from Organic Chemical Wastewater[J].Industrial Water Treatment,2016,36(8):52-54.
[18]周笑犁,王瑞,雷霁卿,等.蓝莓采后病原真菌分离及其生物学鉴定[J].食品科技,2015,40(9):283-293.ZHOU Xiaoli,WANG Rui,LEI Jiqing,et al.Separation and Biological Identification of Pathogenic Fungi on Postharvest Blueberry[J].Food Science and Technology,2015,40(9):283-293.
[19]张立阳,张幸怡,张薇,等.产几丁质酶菌株的筛选、鉴定及其对玉米秸秆中优势霉菌的抑制作用[J].动物营养学报,2017,29(3):970-978.ZHANG Liyang,ZHANG Xingyi,ZHANG Wei,et al.Screening and Identification of Chitinase Producing Strain and Its Inhibition Action to Dominant Moulds in Corn Stalk[J].Chinese Journal of Animal Nutrition,2017,29(3):970-978.
[20]李丹,严红光,袁亮.蓝莓果酒专用酵母的筛选、鉴定与性能研究[J].食品与发酵科技,2015,51(5):75-79.LI Dan,YAN Hongguang,YUAN Liang.Screening,Identification and Study of the Fermentation Properties of Yeast Strains for Exclusive Use of Blueberry Wine[J].Food and Fermentation Technology,2015,51(5):75-79.
[21]谭才邓,王文文,朱美娟,等.1株耐高温高盐生香酵母的选育及特性分析[J].食品与发酵工业,2016,42(3):92-96.TAN Caideng,WANG Wenwen,ZHU Meijuan,et al.Screening and Characterization of Aroma Yeast with Thermo-Tolerant and Salt-Tolerance[J].Food and Fermentation Industries,2016,42(3):92-96.