基于核酸适配子的循环肿瘤细胞检测研究进展
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摘要
循环肿瘤细胞(circulating tumor cell,CTC)是从肿瘤病灶脱落后进入血液循环的肿瘤细胞,在肿瘤的微转移检测、病情评估、疗效及预后评判等方面具有重要价值,但因其在血液中数量极少,难以检测,临床应用进展缓慢。近年来,随着生物学技术的不断发展,CTC的富集及检测新方法不断进步,其中基于核酸适配子的富集及检测方法不但能快速高效捕获和无损释放CTC,还能定性、定量分析CTC,甚至能检测出血液中的单个CTC和不同亚群的肿瘤细胞,具有良好的应用前景。
Circulating tumor cells(CTCs) are cancer cells shed from tumor into blood circulation. These cells are valuable in micrometastatic detection, disease assessment, and therapy and prognosis prediction of tumors. However, the clinical application of CTCs progresses slowly due to its rarity in the blood and difficulty for detection. Given the development of biological techniques, scholars have developed several new methods for enrichment and detection of CTCs. Aptamer-based method shows a good prospect in CTC application. In this method, CTCs can be rapidly and efficiently captured, nondestructively released, and qualitatively and quantitatively analyzed. This method can also be used to detect single and sub-groups of CTCs.
Circulating tumor cells(CTCs) are cancer cells shed from tumor into blood circulation. These cells are valuable in micrometastatic detection, disease assessment, and therapy and prognosis prediction of tumors. However, the clinical application of CTCs progresses slowly due to its rarity in the blood and difficulty for detection. Given the development of biological techniques, scholars have developed several new methods for enrichment and detection of CTCs. Aptamer-based method shows a good prospect in CTC application. In this method, CTCs can be rapidly and efficiently captured, nondestructively released, and qualitatively and quantitatively analyzed. This method can also be used to detect single and sub-groups of CTCs.
引文
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[24]Viraka Nellore BP,Kanchanapally R,Pramanik A,et al.Aptamer-conjugated graphene oxide membranes for highly efficient capture and accurate identification of multiple types of circulating tumor cells[J].Bioconjug Chem,2015,26(2):235-242.
[25]Zhao L,Tang C,Xu L,et al.Enhanced and differential capture of circulating tumor cells from lung cancer patients by microfluidic assays using aptamer cocktail[J].Small,2016,12(8):1072-1081.
[26]Labib M,Green B,Mohamadi RM,et al.Aptamer and antisense-mediated two-dimensional isolation of specific cancer cell subpopulations[J].J Am Chem Soc,2016,138(8):2476-2479.
[27]Shen H,Yang J,Chen Z,et al.A novel label-free and reusable electrochemical cytosensor for highly sensitive detection and specific collection of CTCs[J].Biosens Bioelectron,2016,81:495-502.
[2]Tuerk C,Gold L.Systematic evolution of ligands by exponential enrichment:RNA ligands to bacteriophage T4 DNA polymerase[J].Science,1990,249(4968):505-510.
[3]Ellington AD,Szostak JW.In vitro selection of RNA molecules that bind specific ligands[J].Nature,1990,346(6287):818-822.
[4]Li J,Lei P,Ding S,et al.An enzyme-free surface plasmon resonance biosensor for real-time detecting micro RNA based on allosteric effect of mismatched catalytic hairpin assembly[J].Biosens Bioelectron,2016,77:435-441.
[5]Taghdisi SM,Danesh NM,Ramezani M,et al.A novel electrochemical aptasensor based on Y-shape structure of dual-aptamer-complementary strand conjugate for ultrasensitive detection of myoglobin[J].Biosens Bioelectron,2016,80:532-537.
[6]Sun C,Zhang R,Gao M,et al.A rapid and simple method for efficient capture and accurate discrimination of circulating tumor cells using aptamer conjugated magnetic beads and surface-enhanced Raman scattering imaging[J].Anal Bioanal Chem,2015,407(29):8883-8892.
[7]Wan Y,Liu Y,Allen PB,et al.Capture,isolation and release of cancer cells with aptamer-functionalized glass bead array[J].Lab Chip,2012,12(22):4693-4701.
[8]Li S,Chen N,Zhang Z,et al.Endonuclease-responsive aptamerfunctionalized hydrogel coating for sequential catch and release of cancer cells[J].Biomaterials,2013,34(2):460-469.
[9]Zhang J,Li S,Liu F,et al.SELEX aptamer used as a probe to detect circulating tumor cells in peripheral blood of pancreatic cancer patients[J].PLo S One,2015,10(3):e0121920.
[10]Zamay GS,Kolovskaya OS,Zamay TN,et al.Aptamers selected to postoperative lung adenocarcinoma detect circulating tumor cells in human blood[J].Mol Ther,2015,23(9):1486-1496.
[11]Bai LL,Yang YL,Wang C.Nanotechnology of circulating tumor cell enrichment and detection[J].Progr Biochem Biophysics,2013,40(10):955-962.[白林灵,杨延莲,王琛.循环肿瘤细胞富集和检测的纳米技术[J].生物化学与生物物理进展,2013,40(10):955-962.]
[12]Wan Y,Mahmood MA,Li N,et al.Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology[J].Cancer,2012,118(4):1145-1154.
[13]Wang Y,Zhou F,Liu X,et al.Aptamer-modified micro/nanostructured surfaces:efficient capture of Ramos cells in serum environment[J].ACS Appl Mater Interfaces,2013,5(9):3816-3823.
[14]Zheng F,Cheng Y,Wang J,et al.Aptamer-functionalized barcode particles for the capture and detection of multiple types of circulating tumor cells[J].Adv Mater,2014,26(43):7333-7338.
[15]Xu Y,Phillips JA,Yan J,et al.Aptamer-based microfluidic device for enrichment,sorting,and detection of multiple cancer cells[J].Anal Chem,2009,81(17):7436-7442.
[16]Wan Y,Tan J,Asghar W,et al.Velocity effect on aptamer-based circulating tumor cell isolation in microfluidic devices[J].J Phys Chem B,2011,115(47):13891-13896.
[17]Sheng W,Chen T,Tan W,et al.Multivalent DNA nanospheres for enhanced capture of cancer cells in microfluidic devices[J].ACS Nano,2013,7(8):7067-7076.
[18]Liu XQ,Liu WJ,Liu Y.Advances in detection of circulating tumor cells and molecular markers by Cell Search system[J].Chin Clin Oncol,2012,17(2):182-186.[刘晓晴,刘文静,刘毅.Cell Search系统检测循环肿瘤细胞及其分子标记的研究进展[J].临床肿瘤学杂志,2012,17(2):182-186.]
[19]Zeng Z,Tung CH,Zu Y.A cancer cell-activatable aptamer-reporter system for one-step assay of circulating tumor cells[J].Mol Ther Nucleic Acids,2014,3:e184.
[20]Bi S,Ji B,Zhang Z,et al.A chemiluminescence imaging array for the detection of cancer cells by dual-aptamer recognition and bio-barcode nanoprobe-based rolling circle amplification[J].Chem Commun(Camb),2013,49(33):3452-3454.
[21]Qu L,Xu J,Tan X,et al.Dual-aptamer modification generates a unique interface for highly sensitive and specific electrochemical detection of tumor cells[J].ACS Appl Mater Interfaces,2014,6(10):7309-7315.
[22]Wang L,Zheng Q,Zhang Q,et al.Detection of single tumor cell resistance with aptamer biochip[J].Oncol Lett,2012,4(5):935-940.
[23]Hu B,Lin YF,Liu YY,et al.Application progress of graphene oxide and aptamer in detection[J].Chin Biological Pharms,2015,35(8):186-188.[胡波,林彦锋,刘宇洋,等.氧化石墨烯与核酸适配体在检测方面应用的进展[J].中国生化药物杂志,2015,35(8):186-188.]
[24]Viraka Nellore BP,Kanchanapally R,Pramanik A,et al.Aptamer-conjugated graphene oxide membranes for highly efficient capture and accurate identification of multiple types of circulating tumor cells[J].Bioconjug Chem,2015,26(2):235-242.
[25]Zhao L,Tang C,Xu L,et al.Enhanced and differential capture of circulating tumor cells from lung cancer patients by microfluidic assays using aptamer cocktail[J].Small,2016,12(8):1072-1081.
[26]Labib M,Green B,Mohamadi RM,et al.Aptamer and antisense-mediated two-dimensional isolation of specific cancer cell subpopulations[J].J Am Chem Soc,2016,138(8):2476-2479.
[27]Shen H,Yang J,Chen Z,et al.A novel label-free and reusable electrochemical cytosensor for highly sensitive detection and specific collection of CTCs[J].Biosens Bioelectron,2016,81:495-502.