氧化石墨烯在适配体筛选及医学应用方面的研究进展
|
摘要
SELEX是一种较为成熟的适配体筛选技术,在筛选过程中也涌现出了一系列的筛选方法,如磁珠法、硝酸纤维素法、毛细管电泳法、细胞法等。本文介绍了一种更加快速准确的筛选方法——氧化石墨烯法(graphene oxide,GO-SELEX)。氧化石墨烯具有独特的物理化学性质,筛选效率更高,在对小分子的适配体筛选方面具有其独特的优势。另外,基于氧化石墨烯的适配体目前也逐渐应用于药物靶向运输、肿瘤细胞检测、病原体或生化指标检测等领域,在医学方面具有广阔的应用前景。
Systematic evolution of ligands by exponential enrichment(SELEX) is a relatively mature technology of screening aptamers now.A series of screening methods have also been developed,such as magnetic beads,cellulose nitrate method,capillary electrophoresis,cell SELEX,etc.This article reviews a more rapid and accurate screening method by using graphene oxide(GO-SELEX).Graphene oxide has its unique physical and chemical properties,and its screening efficiency is higher,showing advantages over the aptamer screening for small molecules.In addition,aptamers based on graphene oxide are also gradually applied in drug targeting transport,detection of tumor cells,pathogens or biochemical indicators and other fields,and have promising applications in medicine.
Systematic evolution of ligands by exponential enrichment(SELEX) is a relatively mature technology of screening aptamers now.A series of screening methods have also been developed,such as magnetic beads,cellulose nitrate method,capillary electrophoresis,cell SELEX,etc.This article reviews a more rapid and accurate screening method by using graphene oxide(GO-SELEX).Graphene oxide has its unique physical and chemical properties,and its screening efficiency is higher,showing advantages over the aptamer screening for small molecules.In addition,aptamers based on graphene oxide are also gradually applied in drug targeting transport,detection of tumor cells,pathogens or biochemical indicators and other fields,and have promising applications in medicine.
引文
[1]满燕,吕雪飞,张玉奎,等.核酸适配体及其在生物医学研究中的应用.航天医学与医学工程,2013,26(6):485-490
[2]詹少兵,曾毅.SELEX技术及近年研究进展.病毒学报,2013(5):573-577
[3]Chen Y,Chen Z,Wang Y.Immobilized magnetic beadsbased multi-target affinity selection coupled with HPLCMS for screening active compounds from traditional chinese medicine and natural products.Methods Mol Biol,2015,1286:121-129
[4]Eaton RM,Shallcross JA,Mael LE,et al.Selection of DNA aptamers for ovarian cancer biomarker HE4 using CE-SELEX and high-throughput sequencing.Anal Bioanal Chem,2015,407(23):6965-6973
[5]Kwon HM,Lee KH,Han BW,et al.An RNA aptamer that specifically binds to the glycosylated hemagglutinin of avian influenza virus and suppresses viral infection in cells.Plo S One,2014,9(5):e97574
[6]Champanhac C,Teng I,Cansiz S,et al.Development of a panel of DNA aptamers with high affinity for pancreatic ductal adenocarcinoma.Sci Rep,2015,5:16788.
[7]Park JW,Tatavarty R,Kim DW,et al.Immobilization-free screening of aptamers assisted by graphene oxide.Chem Commun,2012,48(15):2071-2073
[8]Wu M,Kempaiah R,Huang PJ,et al.Adsorption and desorption of DNA on graphene oxide studied by fluorescently labeled oligonucleotides.Langmuir,2011,27(6):2731-2738
[9]Nguyen VT,Kwon YS,Kim JH,et al.Multiple GO-SELEX for efficient screening of flexible aptamers.Chem Commun,2014,50(72):10513-10516
[10]Gao S,Hu B,Zheng X,et al.Gonyautoxin 1/4 aptamers with high-affinity and high-specificity:from efficient selection to aptasensor application.Biosens Bioelectron,2016,79:938-944
[11]Ueno Y,Furukawa K,Matsuo K,et al.On-chip graphene oxide aptasensor for multiple protein detection.Anal Chim Acta,2015,866:1-9
[12]Mo R,Jiang T,Sun W,et al.ATP-responsive DNAgraphene hybrid nanoaggregates for anticancer drug delivery.Biomaterials,2015,50(1):67-74
[13]Tang Y,Hu H,Zhang MG,et al.An aptamer-targeting photoresponsive drug delivery system using"off-on"graphene oxide wrapped mesoporous silica nanoparticles.Nanoscale,2015,7(14):6304-6310
[14]Alibolandi M,Mohammadi M,Taghdisi SM,et al.Fabrication of aptamer decorated dextran coated nano-graphene oxide for targeted drug delivery.Carbohydr Polym,2016,155:218-229
[15]Viraka Nellore BP,Kanchanapally R,Pramanik A,et al.Aptamer-conjugated graphene oxide membranes for highly efficient capture and accurate identification of multiple types of circulating tumor cells.Bioconjug Chem,2011,26(2):235-242
[16]Chen X,Huang Y,Duan N,et al.Screening and identification of DNA aptamers against T-2 toxin assisted by graphene oxide.J Agric Food Chem,2014,62(42):10368-10374
[17]Huang RC,Chiu WJ,Lai PJ,et al.Multivalent aptamer/gold nanoparticle–modified graphene oxide for mass spectrometry–based tumor tissue imaging.Sci Rep,2015,5:10292
[18]Pramanik A,Chavva SR,Fan Z,et al.Extremely high two-photon absorbing graphene oxide for imaging of tumor cells in the second biological window.J Phys Chem Lett,2014,5(12):2150-2154
[19]Xiao K,Liu J,Chen H,et al.A label-free and high-efficient GO-based aptasensor for cancer cells based on cyclic enzymatic signal amplification.Biosens Bioelectron,2017,91:76-81
[20]Green NS,Norton ML.Interactions of DNA with graphene and sensing applications of graphene field-effect transistor devices:a review.Anal Chim Acta,2015,853(1):127-142
[21]Song J,Lau PS,Liu M,et al.A general strategy to create RNA aptamer sensors using"Regulated"graphene oxide adsorption.ACS Appl Mater Interfaces,2014,6(24):21806-21812
[22]Ma X,Jiang Y,Jia F,et al.An aptamer-based electrochemical biosensor for the detection of Salmonella.J Microbiol Methods,2014,98(1):94-98
[23]He C,Xu Z,Sun T,et al.Sensitive electrochemical aptasensor for thrombin detection based on graphene served as platform and graphene oxide as enhancer.Appl Biochem Biotechnol,2014,172(2):1-9
[24]Xia N,Wang X,Liu L.A graphene oxide-based fluorescent method for the detection of human chorionic gonadotropin.Sensors,2016,16(10):1699
[25]Pieper H,Chercheja S,Eigler S,et al.Endoperoxides revealed as origin of the toxicity of graphene oxide.Angew Chem Int Ed Engl,2015,55(1):405
[26]Pelin M,Fusco L,León V,et al.Differential cytotoxic effects of graphene and graphene oxide on skin keratinocytes.Sci Rep,2017,7:40572
[2]詹少兵,曾毅.SELEX技术及近年研究进展.病毒学报,2013(5):573-577
[3]Chen Y,Chen Z,Wang Y.Immobilized magnetic beadsbased multi-target affinity selection coupled with HPLCMS for screening active compounds from traditional chinese medicine and natural products.Methods Mol Biol,2015,1286:121-129
[4]Eaton RM,Shallcross JA,Mael LE,et al.Selection of DNA aptamers for ovarian cancer biomarker HE4 using CE-SELEX and high-throughput sequencing.Anal Bioanal Chem,2015,407(23):6965-6973
[5]Kwon HM,Lee KH,Han BW,et al.An RNA aptamer that specifically binds to the glycosylated hemagglutinin of avian influenza virus and suppresses viral infection in cells.Plo S One,2014,9(5):e97574
[6]Champanhac C,Teng I,Cansiz S,et al.Development of a panel of DNA aptamers with high affinity for pancreatic ductal adenocarcinoma.Sci Rep,2015,5:16788.
[7]Park JW,Tatavarty R,Kim DW,et al.Immobilization-free screening of aptamers assisted by graphene oxide.Chem Commun,2012,48(15):2071-2073
[8]Wu M,Kempaiah R,Huang PJ,et al.Adsorption and desorption of DNA on graphene oxide studied by fluorescently labeled oligonucleotides.Langmuir,2011,27(6):2731-2738
[9]Nguyen VT,Kwon YS,Kim JH,et al.Multiple GO-SELEX for efficient screening of flexible aptamers.Chem Commun,2014,50(72):10513-10516
[10]Gao S,Hu B,Zheng X,et al.Gonyautoxin 1/4 aptamers with high-affinity and high-specificity:from efficient selection to aptasensor application.Biosens Bioelectron,2016,79:938-944
[11]Ueno Y,Furukawa K,Matsuo K,et al.On-chip graphene oxide aptasensor for multiple protein detection.Anal Chim Acta,2015,866:1-9
[12]Mo R,Jiang T,Sun W,et al.ATP-responsive DNAgraphene hybrid nanoaggregates for anticancer drug delivery.Biomaterials,2015,50(1):67-74
[13]Tang Y,Hu H,Zhang MG,et al.An aptamer-targeting photoresponsive drug delivery system using"off-on"graphene oxide wrapped mesoporous silica nanoparticles.Nanoscale,2015,7(14):6304-6310
[14]Alibolandi M,Mohammadi M,Taghdisi SM,et al.Fabrication of aptamer decorated dextran coated nano-graphene oxide for targeted drug delivery.Carbohydr Polym,2016,155:218-229
[15]Viraka Nellore BP,Kanchanapally R,Pramanik A,et al.Aptamer-conjugated graphene oxide membranes for highly efficient capture and accurate identification of multiple types of circulating tumor cells.Bioconjug Chem,2011,26(2):235-242
[16]Chen X,Huang Y,Duan N,et al.Screening and identification of DNA aptamers against T-2 toxin assisted by graphene oxide.J Agric Food Chem,2014,62(42):10368-10374
[17]Huang RC,Chiu WJ,Lai PJ,et al.Multivalent aptamer/gold nanoparticle–modified graphene oxide for mass spectrometry–based tumor tissue imaging.Sci Rep,2015,5:10292
[18]Pramanik A,Chavva SR,Fan Z,et al.Extremely high two-photon absorbing graphene oxide for imaging of tumor cells in the second biological window.J Phys Chem Lett,2014,5(12):2150-2154
[19]Xiao K,Liu J,Chen H,et al.A label-free and high-efficient GO-based aptasensor for cancer cells based on cyclic enzymatic signal amplification.Biosens Bioelectron,2017,91:76-81
[20]Green NS,Norton ML.Interactions of DNA with graphene and sensing applications of graphene field-effect transistor devices:a review.Anal Chim Acta,2015,853(1):127-142
[21]Song J,Lau PS,Liu M,et al.A general strategy to create RNA aptamer sensors using"Regulated"graphene oxide adsorption.ACS Appl Mater Interfaces,2014,6(24):21806-21812
[22]Ma X,Jiang Y,Jia F,et al.An aptamer-based electrochemical biosensor for the detection of Salmonella.J Microbiol Methods,2014,98(1):94-98
[23]He C,Xu Z,Sun T,et al.Sensitive electrochemical aptasensor for thrombin detection based on graphene served as platform and graphene oxide as enhancer.Appl Biochem Biotechnol,2014,172(2):1-9
[24]Xia N,Wang X,Liu L.A graphene oxide-based fluorescent method for the detection of human chorionic gonadotropin.Sensors,2016,16(10):1699
[25]Pieper H,Chercheja S,Eigler S,et al.Endoperoxides revealed as origin of the toxicity of graphene oxide.Angew Chem Int Ed Engl,2015,55(1):405
[26]Pelin M,Fusco L,León V,et al.Differential cytotoxic effects of graphene and graphene oxide on skin keratinocytes.Sci Rep,2017,7:40572