UPLC-Q-TOF-MS快速鉴定黄山野生绞股蓝中皂苷类物质
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摘要
为鉴定黄山野生绞股蓝中皂苷类成分,为黄山野生绞股蓝的开发利用提供理论基础。实验选取超高效液相色谱串联四级杆飞行时间质谱联用技术(UPLC-Q-TOF-MS),采用waters Acquity HSS T3色谱柱(2.1 mm×100 mm,1.8μm),以0.1%甲酸水溶液(A)-乙腈(B)为流动相,梯度洗脱。质谱采用电喷雾(ESI)离子源,在正离子模式下采集数据,首先选取4种具有达玛烷型结构的皂苷标准品进行分析,总结达玛烷型皂苷类物质质谱裂解规律及特征离子碎片信息,再依据这些规律快速鉴定黄山野生绞股蓝中的皂苷类物质。从黄山野生绞股蓝中共鉴别出25种皂苷类物质,其中有6个化合物在该植物中属首次发现。
To identify the saponins in wild Gynostemma pentaphyllum of Huangshan which provides theoretical basis for the development and utilization of wild Gynostemma pentaphyllum in Huangshan.Ultra performance liquid chromatography coupled with time-of-fight mass spectrometry(UPLC-Q-TOF-MS) was selected for the study.The separation was performed on waters Acquity HSS T3 column(2.1 mm×100 mm,1.8 μm),with a mobile phase using water containing 0.1% formic acid(A) and acetonitrile(B) for gradient elution.Q-TOF-MS and electrospray ion(ESI) source were applied for the analysis under the positive mode.Firstly,4 saponins with the damanane type structure were selected for analysis.Then,the fragmentation regularity and the characteristic fragments of saponins with the damanane type structure were explored which applied to identify the saponins in wild Gynostemma Pentaphyllum of Huangshan.25 saponins were identified in wild Gynostemma pentaphyllum of Huangshan,including 6 saponins identified for the first time in Gynostemma pentaphyllum.
To identify the saponins in wild Gynostemma pentaphyllum of Huangshan which provides theoretical basis for the development and utilization of wild Gynostemma pentaphyllum in Huangshan.Ultra performance liquid chromatography coupled with time-of-fight mass spectrometry(UPLC-Q-TOF-MS) was selected for the study.The separation was performed on waters Acquity HSS T3 column(2.1 mm×100 mm,1.8 μm),with a mobile phase using water containing 0.1% formic acid(A) and acetonitrile(B) for gradient elution.Q-TOF-MS and electrospray ion(ESI) source were applied for the analysis under the positive mode.Firstly,4 saponins with the damanane type structure were selected for analysis.Then,the fragmentation regularity and the characteristic fragments of saponins with the damanane type structure were explored which applied to identify the saponins in wild Gynostemma Pentaphyllum of Huangshan.25 saponins were identified in wild Gynostemma pentaphyllum of Huangshan,including 6 saponins identified for the first time in Gynostemma pentaphyllum.
引文
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2 Zhang XZ,Su GY,Xia XY,et al.Research progress in hypoglycemic effect of natural dammarane saponins[J].Chin Tradit Herb Drugs(中草药),2016,47:2758-2763.
3 Shu B,Liu ZJ,Qian MZ.Effects of gypenosiedes on vascular smooth muscle cells phenotype in hyperlipidemic rats[J].Nat Prod Res Dev(天然产物研究与开发),2017,29:217-223.
4 Zhang N,Song N,Cao HM,et al.Effect of gypenosides on the prevention and treatment of atherosclerosis by autophagosome[J].Nat Prod Res Dev(天然产物研究与开发),2017,29:2112-2116.
5 Fan DD,Kuang YH,Xiang SX,et al.Research progress in chemical constituents and pharmacological activities of Gynostemma pentaphyllum[J].Chin Pharm J(中国药学杂志),2017,52:342-352.
6 Li L,Fan HY,Dai XT,et al.Advances in pharmacological effects of gypenosides[J].J Jilin Med Coll(吉林医药学院学报),2015,36:147-150.
7 Lu RM,Pan LW,Wei JH,et al.Chemical constituents of Gynostemma pentaphyllum[J].Chin Tradit Herb Drugs(中草药),2014,45:2757-2761.
8 Zhang Z,Li PJ,Yang Y.et al.The summarize of Gynostemma pentaphyllum(Thunb) Makino[J].Food Res Dev(食品研究与开发),2011,32:193-196.
9 Razmovski-Naumovski V,Huang THW,Tran VH,et al.Chemistry and pharmacology of Gynostemma pentaphyllum[J].Phytochemistry Reviews,2005,4:197-219.
10 Yang F,Shi H,Zhang X,et al.Two novel anti-inflammatory 21-nordammarane saponins from tetraploid Jiaogulan(Gynostemma pentaphyllum)[J].J Agric Food Chem,2013,61:12646-12652.
11 Hu L,Chen Z,Xie Y.New triterpenoid saponins from Gynostemma pentaphyllum[J].J Nat Prod,1996,59:1143-1145.
12 Liu X,Ye WC,Xiao WL,et al.Studies on chemical constituents of Gynostemma Pentaphyllum[J].J Chin Pharm Univ(中国药科大学学报),2003,34(1):23-26.
13 Xu ZL,Yang FQ,Xia ZN,et al.Chemical constituents of Gynostemma pentaphyllum[J].Nat Prod Res Dev(天然产物研究与开发),2013,25:1067-1069.
14 Guo YC,Ou YH,He MZ,et al.Identification of saponins in rhizomes of Anemone davidii by UPLC/Q-TOF-MS/MS[J].Chin Tradit Herb Drugs(中草药),2014,45:1378-1387.
15 Li W,Feng YL,Li TE,et al.Rapid analysis on flavonoids in Glechoma longituba(Nakai) Kupr by UPLC-Q-TOF/MS couple with diagnostic ions[J].J Chin Mass Spectr Soc(质谱学报),2016,37:504-516.
16 Zhou YN,Zhao L,Zheng L,et al.Rapid identification of chemical constituents in Salvia chinensis by HPLC-TOF-MS[J].China J Chin Mater Med(中国中药杂志),2013,38:4109-4112.
17 Zhu YX,Chen LL,Gong QR,et al.Identification of constituents in Suanzaoren tang by LC-Q-TOF-MS and LC-IT-MS[J].China J Chin Mater Med(中国中药杂志),2014,39:457-465.
18 Wang Q,Lu ZW,Liu YH,et al.Rapid analysis on phenolic compounds in Rheum palmatum based on UPLC-Q-TOF /MSE combined with diagnostic ions filter[J].China J Chin Mater Med(中国中药杂志),2017,42:1922-1931.
19 Zhao J,Qin ZX,Peng B,et al.Fragmentation pathway of ginsenosides in Panaxnotoginseng using electrospray ionization-quadrupole / time-of-flight mass spectrometer[J].J Chin Mass Spectr Soc(质谱学报),2017,38:97-108.