胆固醇结石模型豚鼠灌胃给药方法探讨
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摘要
目的介绍改良后的豚鼠灌胃方法,为其他实验者在豚鼠灌胃方面提供借鉴。方法普通级豚鼠40只,雌雄各半,在基于CCKA受体介导的G蛋白信号通路的胆囊胆固醇结石肝郁湿热分子生物学机制研究中建立胆固醇结石模型造模成功后,运用了将灌胃针插入实验动物食管的灌胃方法(传统灌胃方法)及将灌胃针插入实验动物嘴角推入药物由其自主吞咽的灌胃方法(改良后的灌胃方法),监测运用两种灌胃方法灌胃后豚鼠的死亡率。结果与传统灌胃方法组比较,改良后的灌胃方法的豚鼠死亡率明显偏低(P<0.01)。结论改良后的灌胃方法灌胃成功率高,实用性强,值得推广应用。
Objective The method of intragastric administration of improved guinea pigs was introduced,which can be used as reference for other guinea pigs gavage. Methods 40 ordinary guinea pigs,male and female half,based on the CCKA receptor-mediated G protein signaling pathway in the gallstones of cholesterol gallstones in the study of the mechanism of the establishment of cholesterol gallstones,after the modeling succeed,the use of the stent into the experimental animal esophageal feeding method(traditional gavage method) and the use of the intragastric needle into the experimental animal mouth into the drug by its own swallowing method(improved aftergrowth method),the mortality of guinea pigs after intragastric administration of two different gavage methods was monitored. Results Compared with the traditional gavage method, the mortality of the guinea pigs treated with the improved intragastric method was significantly lower(P <0.01). Conclusion The improved gastric gavage method has high success rate and practicability,so it is worth popularizing.
Objective The method of intragastric administration of improved guinea pigs was introduced,which can be used as reference for other guinea pigs gavage. Methods 40 ordinary guinea pigs,male and female half,based on the CCKA receptor-mediated G protein signaling pathway in the gallstones of cholesterol gallstones in the study of the mechanism of the establishment of cholesterol gallstones,after the modeling succeed,the use of the stent into the experimental animal esophageal feeding method(traditional gavage method) and the use of the intragastric needle into the experimental animal mouth into the drug by its own swallowing method(improved aftergrowth method),the mortality of guinea pigs after intragastric administration of two different gavage methods was monitored. Results Compared with the traditional gavage method, the mortality of the guinea pigs treated with the improved intragastric method was significantly lower(P <0.01). Conclusion The improved gastric gavage method has high success rate and practicability,so it is worth popularizing.
引文
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[16]鄢海燕,邹纯才,杨洋,等.小鼠最大灌胃量的研究[J].中国当代医药,2015,22(2):4-6.
[2]魏玉,几种实验动物灌胃给药方法简介[J].上海实验动物科学,2003,23(3):180.
[3]戴支凯.大鼠及小鼠灌胃给药操作方法探讨[J].现代医药卫生,2015,31(11):1738-1740.
[4]赵明德.常用小型实验动物灌胃给药技术方法介绍[J].泸州医学院学报,2004,27(3):258-259.
[5]王丽平,陈芳,刘琴,等.一种简易的实验兔灌胃方法[J].中国比较医学杂志,2015,25(9):50.
[6]王志芳,周攀豪,卢运勤,等.一种新的实验大鼠捉拿、灌胃方法[J].东方食疗与保健,2015(10):235.
[7]姚思君,刘香君,丁春慧,等.医学实验动物大鼠灌胃给药方法的改进[J].中国保健营养,2016,26(6):34.
[8]方远书,何忠平,裘颖儿.实验小鼠的灌胃给药技巧[J].山东畜牧兽医,2014,35(10):90.
[9]邱灿华,李红宇,林文健,等.实验动物常用灌胃方法及改进[J].医学信息,2013,26(6):438-439.
[10]从宁,韩朝,迟放鲁,等.改良的豚鼠灌胃给药方法[J].复旦学报(医学版),2010,37(2):232-235.
[11]袁普卫,王淞,张东,等.豚鼠灌胃方法新改进[J].医学动物防制,2014,30(12):1368-1369.
[12]孙敬方.动物实验方法学[M].北京:人民卫生出版社,2001:164-213.
[13]王太一,韩子玉.实验动物解剖图谱[M].沈阳:辽宁美术出版社,辽宁科学技术出版社,2000:325-326.
[14]李凤奎,王纯耀.实验动物与动物实验方法学[M].郑州:郑州大学出版社,2007:307-322.
[15]胡建华,姚明,崔淑芳.实验动物学教程[M].上海:上海科学技术出版社,2009:149-159.
[16]鄢海燕,邹纯才,杨洋,等.小鼠最大灌胃量的研究[J].中国当代医药,2015,22(2):4-6.