谷树叶不同提取物对高脂血症大鼠血脂及血管内皮细胞Bax和Bcl-2表达的影响
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摘要
目的探讨不同谷树叶提取物对高脂血症模型大鼠血脂水平及血管内皮细胞Bax和Bcl-2表达的影响。方法选取60只正常雄性SD大鼠,随机分为正常对照组、高脂血症模型对照组、辛伐他汀组及谷树叶不同提取物组(水提组、水提醇沉组和醇提组),每组10只;高脂饲料喂养8周造模,造模同时分别进行相应药物干预。分别于第4周和第8周检测大鼠血脂各项指标的水平,并检测血管内皮细胞Bax和Bcl-2的表达。结果与模型组大鼠比较,谷树叶各提取物组的TC和LDL-C水平均降低,HDL-C的水平升高,谷树叶水提醇沉组的作用最为显著。谷树叶不同提取物组均可以不同程度地下调Bax蛋白表达、上调Bcl-2蛋白表达。结论谷树叶提取物能够降低高脂血症大鼠的血脂,并可以通过调节凋亡蛋白Bax和Bcl-2的表达来对抗高脂模型大鼠所致的血管内皮细胞凋亡。
Objective To study the effect of extracts from Broussonetia papyrifera( L.) leaves on blood lipid of rats with hyperlipidemia and the expression of Bax,Bcl-2 in vascular endothelial cells. Methods 60 male SD rats were randomly divided into normal control group,model control group,fenofibrate group and extracts groups( water extraction,water extraction and alcohol precipitation,alcohol extraction),with 10 rats in each group. The models were established by high fat emulsion intragastrically and intervened with corresponding medicine for8 weeks. The levels of blood lipid were detected at 4 thand 8 thweek. The expression of Bax and Bcl-2 in vascular endothelial cells were detected. Results Compared with model group,TC and LDL-C decreased in extracts groups,and the level of HDL-C increased. The group of water extraction and alcohol precipitation has the most significant effect. The different extracts of Broussonetia folium groups had different degrees of down regulation of Bax protein expression and up regulation of Bcl-2 protein expression. Conclusion The extracts from Broussonetia papyrifera( L.) leaves can reduce the blood lipid level of hyperlipidemia and reduce the apoptosis of vascular endothelial cells in rats with hyperlipidemia through regulation of Bax and Bcl-2 expression.
Objective To study the effect of extracts from Broussonetia papyrifera( L.) leaves on blood lipid of rats with hyperlipidemia and the expression of Bax,Bcl-2 in vascular endothelial cells. Methods 60 male SD rats were randomly divided into normal control group,model control group,fenofibrate group and extracts groups( water extraction,water extraction and alcohol precipitation,alcohol extraction),with 10 rats in each group. The models were established by high fat emulsion intragastrically and intervened with corresponding medicine for8 weeks. The levels of blood lipid were detected at 4 thand 8 thweek. The expression of Bax and Bcl-2 in vascular endothelial cells were detected. Results Compared with model group,TC and LDL-C decreased in extracts groups,and the level of HDL-C increased. The group of water extraction and alcohol precipitation has the most significant effect. The different extracts of Broussonetia folium groups had different degrees of down regulation of Bax protein expression and up regulation of Bcl-2 protein expression. Conclusion The extracts from Broussonetia papyrifera( L.) leaves can reduce the blood lipid level of hyperlipidemia and reduce the apoptosis of vascular endothelial cells in rats with hyperlipidemia through regulation of Bax and Bcl-2 expression.
引文
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[7]邓志军,刘若轩,李常青,等.毛蕊花糖苷对高脂模型大鼠血脂及血管内皮细胞Bax-Bcl2表达的影响[J].广东药学院学报,2015,31(5):625-628.
[8]朱开梅,陈丹,李美波,等.构树叶总黄酮调控Bcl-2与Bax蛋白表达及caspase-3活性诱导Hep G-2细胞凋亡的研究[J].中国实验方剂学杂志,2014,20(19):128-133.
[9]孙冬梅,江洁怡,董玉娟,等.布渣叶不同提取物降血脂活性研究[J].辽宁中医药大学学报,2014,16(11):5-7.
[10]张庭廷,童希琼,刘锡云.大蒜素降血脂作用及其机理研究[J].中国实验方剂学杂志,2007,13(2):32-35.
[11]徐丽萍.桔梗总皂甙降血脂作用的研究[J].食品工业科技,2007(8):224,236.
[2]梁·陶弘景.名医别录[M].北京:人民卫生出版社,1986:414.
[3]《中华本草》编委会.中华本草[M]. 2版.上海:上海科学技术出版社,1999:470-474.
[4]王红霞,陈随清.构叶的生药学研究[J].北京中医药大学学报,2008,31(6):415-419.
[5]熊山,叶祖光.楮实子化学成分及药理作用研究进展[J].中国中医药信息杂志,2009,16(5):102-103.
[6]钱俊青,戴承恩,李尚谦,等.竹叶黄酮降血脂活性研究[J].浙江工业大学学报,2014,42(5):496-498,548.
[7]邓志军,刘若轩,李常青,等.毛蕊花糖苷对高脂模型大鼠血脂及血管内皮细胞Bax-Bcl2表达的影响[J].广东药学院学报,2015,31(5):625-628.
[8]朱开梅,陈丹,李美波,等.构树叶总黄酮调控Bcl-2与Bax蛋白表达及caspase-3活性诱导Hep G-2细胞凋亡的研究[J].中国实验方剂学杂志,2014,20(19):128-133.
[9]孙冬梅,江洁怡,董玉娟,等.布渣叶不同提取物降血脂活性研究[J].辽宁中医药大学学报,2014,16(11):5-7.
[10]张庭廷,童希琼,刘锡云.大蒜素降血脂作用及其机理研究[J].中国实验方剂学杂志,2007,13(2):32-35.
[11]徐丽萍.桔梗总皂甙降血脂作用的研究[J].食品工业科技,2007(8):224,236.