摘要
通过研究枸杞多糖(LBP)对免疫抑制小鼠血清中细胞因子IL-6、IL-12/IL-23 p40和IL-10分泌的影响,探讨LBP的免疫调节机制,为LBP的开发利用提供理论依据。60只ICR雌性小鼠,随机分为5组,对各组小鼠进行连续4 d每日腹腔注射环磷酰胺(40 mg/kg),间隔3 d后,再连续4 d每日腹腔注射环磷酰胺(40mg/kg);同时,用高(40 mg/1 kg)、中(20 mg/1 kg)、低剂量(10 mg/1 kg)的LBP给小鼠连续灌胃2周,阳性对照和阴性对照分别用LPS(1 mg/kg)和PBS,连续2周。小鼠眼眶取血,分离血清,ELISA检测血清中IL-6、IL-12/IL-23 p40和IL-10的分泌水平。结果显示,与阳性对照和阴性对照组相比,LBP能提高免疫抑制小鼠血清中IL-6、IL-12/IL-23 p40和IL-10的分泌水平,提示LBP能增强小鼠细胞免疫与体液免疫应答,为枸杞多糖作为免疫增强剂的开发应用奠定了一定的理论基础。
The effects of Lycium barbarum polysaccharides(LBP) on the secretion levels of cytokines including IL-6,IL-12/IL-23 p40,IL-10 in immunosuppressed mice were studied to investigate the immunomodulatory mechanism of LBP for providing the theoretical basis for the utilization of LBP.60 ICR female mice were randomly divided into 5 groups.The mice were intraperitoneally injected with cyclophosphamide for 4 days,3 days later,cyclophosphamide was administered intraperitoneally for 4 days again.At the same time,the mice were fed with high(40 mg/1 kg),medium(20 mg/1 kg) and low(10 mg/1 kg) doses of LBP for 2 weeks.The mice in positive control and negative control groups were treated with LPS(1 mg/kg) and PBS for 2 weeks respectively.Blood was collected from mouse orbit and serum was separated.The secretions of IL-6,IL-12/IL-23 p40 and IL-10 in sera were detected by ELISA.The results showed that,compared with the positive and negative control groups,LBP could improve the secretions of IL-6,IL-12/IL-23 p40 and IL-10 in the sera of immunosuppressed mice,suggesting that LBP can enhance the cellular and humoral immune responses of mice.It provided a basis for the development and application of Lycium barbarum polysaccharide as an immune enhancement agent.
引文
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