枸杞多糖对免疫抑制小鼠血清中IL-6、IL-10和IL-12/IL23 p40分泌水平的影响
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摘要
通过研究枸杞多糖(LBP)对免疫抑制小鼠血清中细胞因子IL-6、IL-12/IL-23 p40和IL-10分泌的影响,探讨LBP的免疫调节机制,为LBP的开发利用提供理论依据。60只ICR雌性小鼠,随机分为5组,对各组小鼠进行连续4 d每日腹腔注射环磷酰胺(40 mg/kg),间隔3 d后,再连续4 d每日腹腔注射环磷酰胺(40mg/kg);同时,用高(40 mg/1 kg)、中(20 mg/1 kg)、低剂量(10 mg/1 kg)的LBP给小鼠连续灌胃2周,阳性对照和阴性对照分别用LPS(1 mg/kg)和PBS,连续2周。小鼠眼眶取血,分离血清,ELISA检测血清中IL-6、IL-12/IL-23 p40和IL-10的分泌水平。结果显示,与阳性对照和阴性对照组相比,LBP能提高免疫抑制小鼠血清中IL-6、IL-12/IL-23 p40和IL-10的分泌水平,提示LBP能增强小鼠细胞免疫与体液免疫应答,为枸杞多糖作为免疫增强剂的开发应用奠定了一定的理论基础。
The effects of Lycium barbarum polysaccharides(LBP) on the secretion levels of cytokines including IL-6,IL-12/IL-23 p40,IL-10 in immunosuppressed mice were studied to investigate the immunomodulatory mechanism of LBP for providing the theoretical basis for the utilization of LBP.60 ICR female mice were randomly divided into 5 groups.The mice were intraperitoneally injected with cyclophosphamide for 4 days,3 days later,cyclophosphamide was administered intraperitoneally for 4 days again.At the same time,the mice were fed with high(40 mg/1 kg),medium(20 mg/1 kg) and low(10 mg/1 kg) doses of LBP for 2 weeks.The mice in positive control and negative control groups were treated with LPS(1 mg/kg) and PBS for 2 weeks respectively.Blood was collected from mouse orbit and serum was separated.The secretions of IL-6,IL-12/IL-23 p40 and IL-10 in sera were detected by ELISA.The results showed that,compared with the positive and negative control groups,LBP could improve the secretions of IL-6,IL-12/IL-23 p40 and IL-10 in the sera of immunosuppressed mice,suggesting that LBP can enhance the cellular and humoral immune responses of mice.It provided a basis for the development and application of Lycium barbarum polysaccharide as an immune enhancement agent.
The effects of Lycium barbarum polysaccharides(LBP) on the secretion levels of cytokines including IL-6,IL-12/IL-23 p40,IL-10 in immunosuppressed mice were studied to investigate the immunomodulatory mechanism of LBP for providing the theoretical basis for the utilization of LBP.60 ICR female mice were randomly divided into 5 groups.The mice were intraperitoneally injected with cyclophosphamide for 4 days,3 days later,cyclophosphamide was administered intraperitoneally for 4 days again.At the same time,the mice were fed with high(40 mg/1 kg),medium(20 mg/1 kg) and low(10 mg/1 kg) doses of LBP for 2 weeks.The mice in positive control and negative control groups were treated with LPS(1 mg/kg) and PBS for 2 weeks respectively.Blood was collected from mouse orbit and serum was separated.The secretions of IL-6,IL-12/IL-23 p40 and IL-10 in sera were detected by ELISA.The results showed that,compared with the positive and negative control groups,LBP could improve the secretions of IL-6,IL-12/IL-23 p40 and IL-10 in the sera of immunosuppressed mice,suggesting that LBP can enhance the cellular and humoral immune responses of mice.It provided a basis for the development and application of Lycium barbarum polysaccharide as an immune enhancement agent.
引文
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[3] 郭培培.枸杞多糖的抗肿瘤及其作用机理研究现状[J].北京联合大学学报,2011,25(4):26-29.
[4] 王佳丽,刘丽华.IL-10对肿瘤免疫双向调节的研究进展[J].中国肿瘤生物治疗杂志,2016,23(1):130-134.
[5] 周琳,周光炎,路丽明.IL-10的双向免疫调节作用[J].细胞与分子免疫学杂志,2012,28(10):1100-1102.
[6] Chaudhry A,Samstein R M,Treuting P,et al.Interleukin-10 sinaling in regulatory T cells is required for suppression of Th17 cell-mediated inflammation[J].Immunity,2011,34(4):566-578.
[7] 周鹏,柏琴琴,陈丽丽.白细胞介素-12生物学作用的研究进展[J].中国生物制品学杂志,2017,30(3):325-328.
[8] Gee K,Guzzo C,Che Mat N F,et al.The IL-12 family of cytokines in infection,inflammation and autoimmune disorders[J].Inflamm Allergy Drug Targets,2009,8(1):40-52.
[9] Hernandez-Alcoceba R,Poutou J,Ballesteros-Briones M C,et al.Gene therapy approaches against cancer using in vivo and ex vivo gene transfer of interleukin-12[J].Immunotherapy,2016,8(2):179-198.
[10] 徐玢,马小彤.IL-23 的研究进展[J].医学分子生物学杂志,2004,1(5):302-305.
[11] 王鑫,官杰.IL-23与肿瘤关系的研究进展[J].中国医药指南,2017,15(20):29-30.
[12] Lan F,Zhang L,Wu J,et al.IL-23/IL-23R:potential mediator of intestinal tumor progression form adenomatous polyps to colorectal carcinoma[J].Int J Colorectal Dis,2011,26(12):1511-1518.