HPLC法测定婴幼儿营养米粉中泛酸的含量
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摘要
本文建立了利用HPLC法测定婴幼儿米粉中泛酸含量的方法。色谱柱:Kinetex C_(18) 2.6μm,4.6×100 mm;流动相:0.02 mol·L~(-1)磷酸二氢钾溶液∶甲醇=95∶5;流速:1 mL·min~(-1);柱温:35℃;进样量:20μL;二极管矩阵检测器,检测波长200 nm。方法在0.861 2～8.612 1μg·mL~(-1)范围内线性良好,相关系数R~2=0.999 946 6,泛酸加标回收率平均值97.6%,样品测定精密度RSD为2.54%。
A HPLC method was adapted for the assay of pantothenic acid in infant nutrition rice powder. The chromatographic system included a Kinetex C_(18) column(2.6 μm 4.6×100 mm) and a mobile phase consisting of Potassium dihydrogen phosphate aqueous solution and methanol(95 ∶ 5),with a flow rate of 1 mL·min~(-1) and 20 μL sample amount.The system was monitored by diode matrix detector at 200 nm.The system was linear from 0.861 2 ～ 8.612 1 μg·mL~(-1) and the relative coefficient was 0.999 946 6. The average recovery rate was 97.6%, the value of RSD was 2.54%.
A HPLC method was adapted for the assay of pantothenic acid in infant nutrition rice powder. The chromatographic system included a Kinetex C_(18) column(2.6 μm 4.6×100 mm) and a mobile phase consisting of Potassium dihydrogen phosphate aqueous solution and methanol(95 ∶ 5),with a flow rate of 1 mL·min~(-1) and 20 μL sample amount.The system was monitored by diode matrix detector at 200 nm.The system was linear from 0.861 2 ～ 8.612 1 μg·mL~(-1) and the relative coefficient was 0.999 946 6. The average recovery rate was 97.6%, the value of RSD was 2.54%.
引文
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[3]彭爱红,邓清莲,吕禹泽.茜素荷移分光光度法测定泛酸钙[J].分析化学,2005,33(6):897.
[4]杜彦山,张志国,贾云虹,等.高效液相色谱法测定奶粉中的泛酸[J].食品研究与开发,2007,28(6):121-124.
[5]姜金斗,王克新,郝岩平,等.HPLC法测定乳制品中泛酸的含量[J].中国乳品工业,2002,30(2):37-39.
[6]刘琳,刘岩,段小娟,等.高效液相色谱法测定乳及乳制品中泛酸分析方法的改进[J].食品安全质量检测学报,2017,8(11):4190-4194.
[7]何敏恒,李秀英,曾令浩,等.同位素稀释-超高效液相色谱-串联质谱法检测蜂蜜中泛酸含量[J].中国酿造,2015,34(4):157-160.
[8]杨祖英.食品检验[M].北京:化学工业出版社,2002.
[9]刘志楠,喻东威,宋晓东,等.烟酸和泛酸不同方法检测的对比[J].食品研究与开发,2011,32(11):91-93.