补气通络解毒方对胰腺癌模型小鼠肿瘤高甲基化基因1、沉默信息调节因子、Shh蛋白浓度的影响
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摘要
目的观察补气通络解毒方对胰腺癌模型小鼠肿瘤高甲基化基因1(HIC1)、沉默信息调节因子(SIRT_1)、Shh蛋白浓度的影响。方法将30只裸鼠随机分为3组各10只,采用癌细胞种移植法造模。造模成功后,空白组和模型组给予生理盐水灌胃,治疗组给予补气通络解毒方灌胃,连续14 d。治疗结束后,处死裸鼠,取出瘤组织,以WB法检测各组标本的HIC1、SIRT_1、Shh蛋白浓度。结果 3组HIC 1、SIRT 1、Shh蛋白浓度比较有显著差异(P<0.05)。结论补气通络解毒方对模型小鼠HIC1、SIRT_1、Shh蛋白浓度有调节的作用。
Objective To observe the effect of Buqi Tongluo Jiedu decoction on protein concentrations of HIC1、SIRT_1、Shh in model mice with pancreatic cancer. Methods Totally 30 mice were randomly and averagely divided into three groups. Mice with cancer were modeling by cancer cells seeding method. After successfulmodeling,the blank group and model group were given saline for gastric lavage,while the treatment group was given Buqi Tongluo Jiedu decoction for gastric lavage. All the three groups were treated for 14 days. After treatment,the mice were sacrificed for picking up the tumor tissue. Protein concentrations of HIC1、SIRT_1、Shh were detected by WB method.Results There were significant differences in the concentrations of HIC1,SIRT_1 and Shh among the three groups( P < 0. 05). Conclusion Buqi Tongluo Jiedu decoction play an important role in regulating protein concentrations of HIC1、SIRT_1、Shh in model mice with pancreatic cancer.
Objective To observe the effect of Buqi Tongluo Jiedu decoction on protein concentrations of HIC1、SIRT_1、Shh in model mice with pancreatic cancer. Methods Totally 30 mice were randomly and averagely divided into three groups. Mice with cancer were modeling by cancer cells seeding method. After successfulmodeling,the blank group and model group were given saline for gastric lavage,while the treatment group was given Buqi Tongluo Jiedu decoction for gastric lavage. All the three groups were treated for 14 days. After treatment,the mice were sacrificed for picking up the tumor tissue. Protein concentrations of HIC1、SIRT_1、Shh were detected by WB method.Results There were significant differences in the concentrations of HIC1,SIRT_1 and Shh among the three groups( P < 0. 05). Conclusion Buqi Tongluo Jiedu decoction play an important role in regulating protein concentrations of HIC1、SIRT_1、Shh in model mice with pancreatic cancer.
引文
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[12]沈世林,赵健雄,朱玉真,等.复方中药制剂对肿瘤细胞NF-κB表达及凋亡影响的实验研究[J].四川中医,2004,22(7):12-13.
[13]杨勤建,陈振发,雷良蔚,等.化痰散结方对人肺癌SPC2A1细胞内c AMP、c GMP浓度的影响[J].湖北中医学院学报,2003,5(1):17-19.
[14]方志俊,曹佳,敖琳,等.基因芯片分析显示昆明山海棠有机萃取液经NF-κB及线粒体信号传导途径诱导HL-60细胞凋亡[J].中草药,2003,34(4):334-338.
[15]赵玉刚.解毒法在结肠癌治疗中的运用[J].黑龙江中医药,1998,11(3):32-34.
[16]林松挺,陈正义,钟文洲.Shh蛋白在胰腺癌组织中的表达及其临床意义[J].中国普通外科杂志,2016,25(9):1266-1270.
[2]王玉琼,丁佳寅,诸娴,等.炎性因子对人胰腺癌PaTu8988细胞NF-κB及Hedgehog通路成员表达的影响[J].中华胰腺病杂志,2015,15(1):18-21.
[3]Norman J.The role of cytokines in the pathogenesis of acute pancreatitis[J].American Journal of Surgery,1998,175(1):76-83.
[4]胡波,王春友.补气通络解毒方对胰腺癌模型小鼠HIC1/SIRT1基因表达的实验研究[J].医师在线,2016,16(8):132-134.
[5]苏全武,胡波,王春友.补气通络解毒方含药血清对人Bb PG3胰腺癌细胞抑制作用的研究[J].中国中西医结合消化杂志,2016,24(10):737-740.
[6]Toftgard R.Hedgehog signaling in cancer[J].Cell Mol Life Sci,2000,57:1720-1731.
[7]Kayed H,Kleeff J,Esposito I,et al.Localization ofthe human hedgehog interacting protein(Hip)in the normal and diseased pancreas[J].Mol Carcinog,2005,42:183-192.
[8]Oguro S,Ino Y,Shimada K,et al.Clinical significance of tumorinfiltrating immune cells focusing on BTLA and Cbl-b in patients with gallbladder cancer[J].Caner Sci,2015,106(12):1750-1760.
[9]胡波,周贞迪,邱幸凡,等.补气通络解毒方配合GEMOX方案治疗中晚期胰腺癌60例临床观察[J].北京中医药,2010,29(10):770-772.
[10]刘军,柳惠图,梁云燕,等.复方中药白龙对G1期人胃癌细胞中抑癌基因的影响及与PKA信号通路的相关性[J].中国中西医结合杂志,1999,19(10):613-616.
[11]张军港,石英,王知非,等.Sirtinol对胰腺癌PANC-1细胞增殖和5-FU敏感性的影响[J].世界华人消化杂志,2014,22(1):86-90.
[12]沈世林,赵健雄,朱玉真,等.复方中药制剂对肿瘤细胞NF-κB表达及凋亡影响的实验研究[J].四川中医,2004,22(7):12-13.
[13]杨勤建,陈振发,雷良蔚,等.化痰散结方对人肺癌SPC2A1细胞内c AMP、c GMP浓度的影响[J].湖北中医学院学报,2003,5(1):17-19.
[14]方志俊,曹佳,敖琳,等.基因芯片分析显示昆明山海棠有机萃取液经NF-κB及线粒体信号传导途径诱导HL-60细胞凋亡[J].中草药,2003,34(4):334-338.
[15]赵玉刚.解毒法在结肠癌治疗中的运用[J].黑龙江中医药,1998,11(3):32-34.
[16]林松挺,陈正义,钟文洲.Shh蛋白在胰腺癌组织中的表达及其临床意义[J].中国普通外科杂志,2016,25(9):1266-1270.