激光诱导胃体组织自体荧光诊断早癌的检测系统研究及临床实验
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摘要
激光诱导自体荧光(LIAF)诊断技术是利用正常组织与癌变组织自体荧光光谱及强度存在明显差异来诊断病变,克服常规取样活检容易出现微小病变和较深层病变漏检的缺点,使临床诊断早癌变为现实。该系统在相同条件下对海口市人民医院消化内镜中心41个患者进行在体连续LIAF光谱检测和分析,以磷状细胞癌光谱图为中心分别与正常胃体组织,炎性组织、增生组织等进行横向分析比较查找出其差异性。LIAF光谱检测技术可以明确区分正常组织与癌变组织,实时发现早癌和癌前病变,实现"光活检"。
The laser-induced autofluorescence(LIAF) diagnosis technique can diagnose lesions by using the difference in the autofluorescence spectrum and intensity of normal tissue and cancerous tissue. This technigue overcomes the shortcomings of routine sampling biopsy that are prone to small lesions and deeper lesion missed detection, making clinical diagnosis of early cancer a reality. Under the same conditions, the system performed in vivo LIAF spectroscopic detection and analysis on 41 patients in the Digestive Endoscopy Center of Haikou People's Hospital. Taking the phosphatidyl cancer spectrum as a center, and lateral analysis and comparison with normal body tissues, inflammatory tissues, and hyperplasia tissues revealed differences. LIAF spectral detection technology can clearly distinguish between normal and cancerous tissues, real-time detection of early cancer and precancerous lesions, and achieve "photo biopsy".
The laser-induced autofluorescence(LIAF) diagnosis technique can diagnose lesions by using the difference in the autofluorescence spectrum and intensity of normal tissue and cancerous tissue. This technigue overcomes the shortcomings of routine sampling biopsy that are prone to small lesions and deeper lesion missed detection, making clinical diagnosis of early cancer a reality. Under the same conditions, the system performed in vivo LIAF spectroscopic detection and analysis on 41 patients in the Digestive Endoscopy Center of Haikou People's Hospital. Taking the phosphatidyl cancer spectrum as a center, and lateral analysis and comparison with normal body tissues, inflammatory tissues, and hyperplasia tissues revealed differences. LIAF spectral detection technology can clearly distinguish between normal and cancerous tissues, real-time detection of early cancer and precancerous lesions, and achieve "photo biopsy".
引文
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[2]朱明宇,常玮,廖筑秀,等.荧光诊断技术在临床中的应用与展望[J].医疗卫生装备,2018,39(2):13-17.
[3]沙鹏.消化道早癌诊断的常用内镜及诊断进展[J].医疗装备,2018,31(6):200-201.
[4]张先增,谢树森,林慧韫.生物组织激光消融阀值的光谱特性[J].激光生物学报,2006,15(1):97-100.
[5]赵九龙,杨帆,李兆申.早期食管癌及癌前病变的内镜下筛查和精查进展[J].中华消化内镜杂志,2015,(5):338-340.
[6]蔡世伦,钟芸诗,姚礼庆.放大内镜窄带成像技术在消化道早癌诊断中的应用进展[J].中华消化内镜杂志,2014,31(12):748-753.
[7]张浩,陈世耀.荧光内镜及其在胃肠道肿瘤早期诊断中的应用[J].中华消化内镜杂志,2013,30(4):238-240.
[8]付玲,袁菁,王家福,等.多光谱荧光共聚焦内窥显微成像系统研究[J].中国医疗器械信息,2015,(10):6-9.
[9]李伟军,郭萍,王振华.激光诱导自体荧光谱在肿瘤诊断中的比较研究[J].衡阳师范学院学报,2005,26(3):60-62.
[10]欧辉彬,傅昇.基于自体荧光诊断技术的早期肿瘤检测系统的研究[J].中国医疗设备,2015,30(4):28-31.
[11]叶衍铭,戈之铮,萧树东,等.荧光内镜在医疗诊断中的应用观察[J].中国医疗器械杂志,2013,(6):457-459.
[12]Kusunoki Y,Imamura F,Uda H,et al.Early detection of lung cancer with laser-induced fluorescence endoscopy and spectrofluorometry[J].Chest,2000,118(6):1776.
[13]汤陌生.自体荧光成像技术在乳腺癌和结肠诊断中的应用[J].中国医疗设备,2014,29(4):33-35.
[14]Fu S,Chia TC,Kwek LC,et al.High sensitivity of laser-induced autofluorescence spectra for detection of colorectal cancer with an artiflcial neural network[J].Appl Opt,2005,44(19):4004-4008.
[15]Schleier P.ALA-based fluorescence diagnosis of malignant oral lesions in the presence of bacterial porphyrin formation[J].Proc SPIE,2006,6139:613908-613908-8.
[16]于常青.激光诱导荧光技术在生物医学中的应用研究[D].北京:北京理工大学,2001.
[17]李为光.消化内镜自发荧光技术[J].胃肠病学,2007,12(7):435-438.
[18]谭学滨,马小红.消化内镜技术用于消化道早癌诊断治疗价值研究[J].临床医药文献电子杂志,2018,5(5):14.