不同处理对凤丹牡丹种子萌发的影响
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摘要
开展了凤丹牡丹(Paeonia ostii T.Hong et J.X.Zhang)种子的特性观测并进行了种子分级试验;采用不同浸种温度、不同浸种时间、沙藏层积、不同浓度激素处理凤丹牡丹种子,筛选出能够促进种子萌发的最佳处理方式。结果表明,利用凤丹种子的比重不同,采取水浸法进行种子分级,能有效区分种子优劣,且Ⅱ级种子的发芽率最高,达到99%;赤霉素(GA3)处理能大幅提高播种出苗率,GA3促进种子萌发的的最佳浓度为150 mg/L,种子萌发率达到99%。种子的品质与质量相关性不大,与吸水率呈反比;凤丹牡丹种子萌发需要一定的生理后熟期。
The seed characteristic and the method of seeds grading were conducted; Using different soaking temperature, different soaking time, stratification treatment and different hormone concentration to treat seeds of Paeonia ostii T.Hong et J.X.Zhang, the best treatment method for promoting seed germination was selected. The results showed that the immersion method of the separation of Paeonia ostii T.Hong et J.X.Zhang seeds based on different proportion could effectively distinguish the good seeds and bad seeds, and the germination rate of the second seed was the highest for 99%; GA3 treatments enhanced the rate of the seedling emergence, and the optimal concentration of GA3 for promoting seed germination was 150 mg/L, and the seed germination rate reached up to 99%. The quality of the seed is not related to weight, but inversely proportional to the water absorption; the seed germination of Paeonia ostii T.Hong et J.X.Zhang requires some physiological maturity physiological latter ripe stage.
The seed characteristic and the method of seeds grading were conducted; Using different soaking temperature, different soaking time, stratification treatment and different hormone concentration to treat seeds of Paeonia ostii T.Hong et J.X.Zhang, the best treatment method for promoting seed germination was selected. The results showed that the immersion method of the separation of Paeonia ostii T.Hong et J.X.Zhang seeds based on different proportion could effectively distinguish the good seeds and bad seeds, and the germination rate of the second seed was the highest for 99%; GA3 treatments enhanced the rate of the seedling emergence, and the optimal concentration of GA3 for promoting seed germination was 150 mg/L, and the seed germination rate reached up to 99%. The quality of the seed is not related to weight, but inversely proportional to the water absorption; the seed germination of Paeonia ostii T.Hong et J.X.Zhang requires some physiological maturity physiological latter ripe stage.
引文
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[14]杨期和,叶万辉,宋松泉,等.植物种子休眠的原因及休眠的多形性[J].西北植物学报,2004,23(5):837-843.
[15]景新明,郑光华.栽培牡丹的种子发芽和贮藏特性(简报)[J].植物生理学报,1995,31(4):268-270.
[16]JING X M,ZHENG G H.The Characteristics in Seed Germination and Dormancy of Four Wild Species of Tree Peonies and Their Bearing on Endangerment[J].Acta Photophysiologica Sinica,1999,25(3):214-221.
[17]娄方芳,赵林森,李宗艳.昆明西山野生黄牡丹种子休眠与发芽特性初步研究[J].西南林学院学报,2008,27(5):34-37.
[18]张霁,刘心民.低温解除牡丹种子上胚轴休眠的试验[J].浙江农业科学,2008(3):295-296.
[19]张远兵,刘爱荣,张雪平.不同贮藏方法及激素、稀土等对牡丹种子发芽及幼苗生长的影响[J].种子,2005,24(8):16-20.
[20]侯冬花,萨拉木·艾尼瓦尔,海利力·库尔班.种子休眠与休眠解除的研究进展[J].新疆农业科学,2007,44(3):349-354.
[21]MEYER S E,PENDLETON R L.Genetic Regulation of Seed Dormancy in Purshia tridentate(Rosaceae)[J].Ann Bot-London,2000,85(4):521-529.
[22]闫翠香,丁新泉,宋闪闪,等.悬钩子属植物种子休眠及其解除方法探讨[J].种子,2014,33(3):55-58.
[23]周仁超,姚崇怀,潘俊,等.紫斑牡丹种子休眠和发芽特性初步研究[J].湖北农业科学,2002(1):59-60.
[24]林松明,徐迎春,蔡志仁,等.打破凤丹种子上胚轴休眠的研究[J].江苏农业科学,2006(1):84-86.
[2]朱献标,翟文婷,董秀勋,等.牡丹籽油化学成分及功能研究进展[J].中国油脂,2014,39(1):88-90.
[3]李育材.中国油用牡丹工程战略思考[J].中国工程科学,2014,6(10):58-63.
[4]成仿云,杜秀娟.低温与赤霉素处理对‘凤丹’牡丹种子发芽和幼苗生长的影响[J].园艺学报,2008,35(4):553-558.
[5]张玉刚,郭绍霞,王莲英.牡丹容器育苗的初步研究[J].中国农学通报,2004,20(1):182-184.
[6]曹亚悦,朱再标,郭巧生,等.药用牡丹种子质量分级标准研究[J].中国中药杂志,2015,40(4):624-628.
[7]赵叶,杨瑶君,吴三林,等.浸种时间对凤丹种子发芽的影响[J].基因组学与应用生物学,2015(9):2024-2029.
[8]宋焕芝,于晓南,沈苗苗.芍药属植物种子双重休眠特性与破眠技术研究进展[J].种子,2011,30(3):67-70.
[9]郑相穆,周阮宝,谷丽萍,等.凤丹种子的休眠和萌发特性[J].植物生理学报,1995,31(4):260-262.
[10]P魪REZ-GARC魱A F,ESCUDERO A.Role of the seed coat in germination of Cistus populifolius L.[J].Israel J Plant Sci,1997,45(4):329-331.
[11]LIN C H,LEE L Y,TSENG M J.The effect of stratification and thidiazuron treatment on germination and protein synthesis of Pyrus serotina Rehd cv.Niauli[J].Ann Bot-London,1994,73(5):515-523.
[12]DEBEAUJON I,L魪ON-KLOOSTERZIEL K M,KOORNNEEF M.Influence of the testa on seed dormancy,germination,and longevity in Arabidopsis[J].Plant Physiol,2000,122(2):403-413.
[13]BASKIN J M,BASKIN C C.A classification system for seed dormancy[J].Seed sci Res,2004,14(1):1-16.
[14]杨期和,叶万辉,宋松泉,等.植物种子休眠的原因及休眠的多形性[J].西北植物学报,2004,23(5):837-843.
[15]景新明,郑光华.栽培牡丹的种子发芽和贮藏特性(简报)[J].植物生理学报,1995,31(4):268-270.
[16]JING X M,ZHENG G H.The Characteristics in Seed Germination and Dormancy of Four Wild Species of Tree Peonies and Their Bearing on Endangerment[J].Acta Photophysiologica Sinica,1999,25(3):214-221.
[17]娄方芳,赵林森,李宗艳.昆明西山野生黄牡丹种子休眠与发芽特性初步研究[J].西南林学院学报,2008,27(5):34-37.
[18]张霁,刘心民.低温解除牡丹种子上胚轴休眠的试验[J].浙江农业科学,2008(3):295-296.
[19]张远兵,刘爱荣,张雪平.不同贮藏方法及激素、稀土等对牡丹种子发芽及幼苗生长的影响[J].种子,2005,24(8):16-20.
[20]侯冬花,萨拉木·艾尼瓦尔,海利力·库尔班.种子休眠与休眠解除的研究进展[J].新疆农业科学,2007,44(3):349-354.
[21]MEYER S E,PENDLETON R L.Genetic Regulation of Seed Dormancy in Purshia tridentate(Rosaceae)[J].Ann Bot-London,2000,85(4):521-529.
[22]闫翠香,丁新泉,宋闪闪,等.悬钩子属植物种子休眠及其解除方法探讨[J].种子,2014,33(3):55-58.
[23]周仁超,姚崇怀,潘俊,等.紫斑牡丹种子休眠和发芽特性初步研究[J].湖北农业科学,2002(1):59-60.
[24]林松明,徐迎春,蔡志仁,等.打破凤丹种子上胚轴休眠的研究[J].江苏农业科学,2006(1):84-86.