外源油菜素内酯对低温胁迫下玉米发芽及幼苗生理特性的影响
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摘要
为探究外源油菜素内酯(BR)对低温胁迫下玉米发芽及幼苗生理特性的影响,以天农九号和天和1号玉米杂交种为试验材料,分别用BR浸种和喷施处理后,对2个玉米品种低温处理下的农艺性状和生理特性进行了比较研究。结果表明,外源BR处理能有效缓解低温胁迫对玉米生长的抑制程度。其中0.1 mg/L BR处理效果最好,主要表现在玉米发芽率、幼苗地上及地下部生物量和SPAD值的增加;MDA含量和相对电导率的降低;SOD、POD和CAT活性的增加;脯氨酸、可溶性糖和可溶性蛋白含量的增加;与低温对照比较,玉米杂交种天农九号和天和1号起始发芽率分别提高18.89,11.10百分点,终发芽率分别提高6.67,18.89百分点;地上部干质量增加55.92%,49.15%,地下部干质量增加38.79%,14.26%,相对电导率降低7.33%,6.80%,CAT活性增加110.58%,120.43%,脯氨酸含量增加66.59%,54.63%。说明低温胁迫下喷施适宜浓度的BR对2个玉米品种缓解抑制能力不同,表现为天农九号>天和1号;抗冷性强弱不同的玉米品种对不同浓度BR的适应性存在差异。
In order to explore the effects of brassinolide(BR)on seed germination and seedling physiological characteristics of maize under low temperature stress,the maize hybrids Tiannong 9 and Tianhe 1 were used as the materials to study the changes of agronomic traits and physiological properties after their seeds were soaked or sprayed with BR. The results showed that exogenous BR treatment could effectively alleviate the inhibition of maize growth under low temperature stress. The best treating concentration was 0.1 mg/L BR,which increased the germination rate,the biomass of overground and underground parts,the SPAD value,the activities of SOD,POD and CAT,and the contents of proline,soluble sugar and soluble protein,while decreased the MDA content and the relative conductivity in maize seedlings. Compared with the low temperature control,the maize hybrids Tiannong 9 and Tianhe 1 treated with exogenous BR of 0.1 mg/L initial germination rate increased by 18.89 and 11.10 percentage points respectively, the final germination rate increased by 6.67 and 18.89 percentage points,their aboveground dry weight increased by 55.92% and 49.15%,their underground dry weight raised by 38.79% and 14.26%,their relative electrical conductivity decreased by 7.33% and 6.80%,their CAT activity increased by 110.58% and 120.43%,and their proline content increased by 66.59% and 54.63%,respectively. It suggested that the relieving effects of 0.1 mg/L BR treatment on the two maize varieties under low temperature stress were different,Tiannong 9 being better than Tianhe 1. The maize varieties with different cold resistances had different adaptability to different BR concentrations.
In order to explore the effects of brassinolide(BR)on seed germination and seedling physiological characteristics of maize under low temperature stress,the maize hybrids Tiannong 9 and Tianhe 1 were used as the materials to study the changes of agronomic traits and physiological properties after their seeds were soaked or sprayed with BR. The results showed that exogenous BR treatment could effectively alleviate the inhibition of maize growth under low temperature stress. The best treating concentration was 0.1 mg/L BR,which increased the germination rate,the biomass of overground and underground parts,the SPAD value,the activities of SOD,POD and CAT,and the contents of proline,soluble sugar and soluble protein,while decreased the MDA content and the relative conductivity in maize seedlings. Compared with the low temperature control,the maize hybrids Tiannong 9 and Tianhe 1 treated with exogenous BR of 0.1 mg/L initial germination rate increased by 18.89 and 11.10 percentage points respectively, the final germination rate increased by 6.67 and 18.89 percentage points,their aboveground dry weight increased by 55.92% and 49.15%,their underground dry weight raised by 38.79% and 14.26%,their relative electrical conductivity decreased by 7.33% and 6.80%,their CAT activity increased by 110.58% and 120.43%,and their proline content increased by 66.59% and 54.63%,respectively. It suggested that the relieving effects of 0.1 mg/L BR treatment on the two maize varieties under low temperature stress were different,Tiannong 9 being better than Tianhe 1. The maize varieties with different cold resistances had different adaptability to different BR concentrations.
引文
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[15] Wu X X,Ding H D,Chen J L,Zhu Z W,Zha D S.Amelioration of oxidative damage in Solanum melongena seedlings by 24-epibrassinolide during chilling stress and recovery[J].Biologia Plantarum,2015,59(2):350-356.doi:10.1007/s10535-015-0495-0.
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[17] 李淑叶,马慧娟,张思平,刘绍东,沈倩,陈静,葛常伟,庞朝友,赵新华.外源2,4表油菜素内酯对低温胁迫下棉花幼苗光合生理的影响[J].棉花学报,2018,30(3):252-260.Li S S,Ma H J,Zhang S P,Liu S D,Shen Q,Chen J,Ge C W,Pang C Y,Zhao X H.Effects of exogenous 2,4-epibrassinolide on photosynthetic physiology of cotton seedlings under low temperature [J].Cotton Science,2018,30(3):252-260.
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[20] Xu T.Bioaccumulation,subcellular,and molecular localization and damage to physiology and ultrastructure in Nymphoides peltata (Gmel.)O.Kuntze exposed to yttrium[J].Environmental Science & Pollution Research,2014,21(4):2935-2942.doi:10.1007/s11356-013-2246-0.
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