星点设计-响应面法优化党参总皂苷提取工艺
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摘要
目的:通过星点设计-响应面法优化党参总皂苷提取工艺。方法:选择超声提取法,以浸泡时间、乙醇体积分数、液料比、超声时间和超声温度为影响因素,总皂苷得率为评价指标,采用星点设计-响应面法优化党参总皂苷超声提取的最佳工艺参数。结果:最佳条件为浸泡22.98 h,乙醇体积分数为71.47%,液料比30.47,超声29.59 min,超声温度为39.64℃,此时党参总皂苷得率为1.638%。结论:该方法操作简单、结果精确,预测性良好,可用于党参总皂苷的提取。
Objective:To optimize the extraction process of total saponins from Radix Codonopsis Pilosulae by design and response surface method. Methods:Ultrasonic extraction method was selected. The soaking time,ethanol volume fraction,liquid-to-liquid ratio,ultrasonic time and ultrasonic temperature were the influencing factors. The total saponin yield was evaluated. The optimum parameters of ultrasonic extraction of total saponins from Radix Codonopsis Pilosulae were optimized by central composite design and response surface method. Results:The optimal conditions were soaking time 22.98 h,ethanol volume fraction 71.47%,liquid-solid ratio 30.47,ultrasonic time 29.59 min,ultrasonic temperature 39.64 ℃,and the total saponin yield was 1.638%. Conclusion:This method was simple,accurate and predictable,and could be used for the extraction of total saponins from Radix Codonopsis Pilosulae.
Objective:To optimize the extraction process of total saponins from Radix Codonopsis Pilosulae by design and response surface method. Methods:Ultrasonic extraction method was selected. The soaking time,ethanol volume fraction,liquid-to-liquid ratio,ultrasonic time and ultrasonic temperature were the influencing factors. The total saponin yield was evaluated. The optimum parameters of ultrasonic extraction of total saponins from Radix Codonopsis Pilosulae were optimized by central composite design and response surface method. Results:The optimal conditions were soaking time 22.98 h,ethanol volume fraction 71.47%,liquid-solid ratio 30.47,ultrasonic time 29.59 min,ultrasonic temperature 39.64 ℃,and the total saponin yield was 1.638%. Conclusion:This method was simple,accurate and predictable,and could be used for the extraction of total saponins from Radix Codonopsis Pilosulae.
引文
[1]孙耀贵,程佳,温伟业.党参总皂苷抗氧化作用研究[J].中兽医医药杂志,2010,29(3):37-39.
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[3]周波林.中药有效成分调控Bcl-2基因抗器官缺血-再灌注损伤的研究进展[J].中国药房,2014,(15):1433-1435.
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[6]刘文生.党参多糖、皂甙提取及对小鼠抗氧化和免疫功能影响的研究[D].太原:山西农业大学,2004.
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[9]方志娥.党参总皂苷的提取纯化及对大鼠肾缺血再灌注损伤的保护作用研究[D].重庆:重庆医科大学,2010.
[10]吴伟,崔光华,陆彬.实验设计中多指标的优化:星点设计和总评“归一值”的应用[J].中国药学杂志,2000,35(8):530-533.
[2]方志娥,李艳艳,杨雅淋,等.党参总皂苷对人肝癌SMMC-7721细胞的抑制作用及其机制[J].中国药房,2015,(10):1356-1359.
[3]周波林.中药有效成分调控Bcl-2基因抗器官缺血-再灌注损伤的研究进展[J].中国药房,2014,(15):1433-1435.
[4]李炜,武欣,赵红娜,等.党参总皂苷的提取、含量测定及其抗脂质过氧化作用的研究[C].全国有毒中药的研究及其合理应用交流研讨会,2012.
[5]辜鹏程,方志娥,尚京川.超声提取党参总皂苷的工艺[J].光谱实验室,2012,4(29):2335-2339.
[6]刘文生.党参多糖、皂甙提取及对小鼠抗氧化和免疫功能影响的研究[D].太原:山西农业大学,2004.
[7]蔡雄,刘中秋,王培训,等.大孔吸附树脂富集纯化人参总皂苷工艺[J].中成药,2001,23(9):631-634.
[8]辜鹏程,方志娥,尚京川.超声提取党参总皂苷的工艺[J].光谱实验室,2012,29(4):2335-2339.
[9]方志娥.党参总皂苷的提取纯化及对大鼠肾缺血再灌注损伤的保护作用研究[D].重庆:重庆医科大学,2010.
[10]吴伟,崔光华,陆彬.实验设计中多指标的优化:星点设计和总评“归一值”的应用[J].中国药学杂志,2000,35(8):530-533.