香樟叶挥发油的止痒作用研究
|
摘要
[目的]探讨香樟叶挥发油的止痒作用及其可能的作用机制。[方法]采用4-氨基吡啶诱导小鼠瘙痒模型,2,4-二硝基氟苯诱导小鼠变态反应性接触性皮炎模型,探讨香樟叶挥发油的止痒作用。[结果] 4-氨基吡啶瘙痒模型中,与模型组相比,香樟叶挥发油高、低剂量10 min内舔体次数均显著减少(P<0.01),香樟叶挥发油高剂量组小鼠血清白三烯B4(LTB4)和5-羟色胺(5-HT)的水平显著降低(P<0.05),但香樟叶挥发油各组对白细胞介素-4(IL-4)均无显著性影响(P>0.05)。病理检查显示模型组出现大量炎细胞,香樟叶挥发油高、低剂量组未见炎细胞,挥发油低剂量组真皮层轻度增厚。2,4-二硝基氟苯变态反应性接触性皮炎模型中,与模型组相比,香樟叶挥发油高剂量组小鼠血清LTB4的水平显著降低(P<0.05),但香樟叶挥发油各组对IL-4均无显著性影响(P>0.05)。[结论]香樟叶挥发油具有显著的止痒作用,降低瘙痒小鼠血清中5-HT和LTB4的量是其作用机制之一。
[Objective] To study the antipruritic effect of essential oil of Cinnamomum camphora leaves and its possible mechanism.[Methods] The model of pruritus induced by 4-aminopyridine in mice and the model of allergic contact dermatitis induced by 2,4-dinitrofluorobenzene in mice were explored. The antipruritic effect of essentia oil of Cinnamomum camphora leaves was investigated.[Results] In 4-aminopyridine pruritus model,compared with the model group the number of licking body in high and low doses of essential oil group was significantly decreased in 10 min(P<0.01). The contents of LTB4 and 5-HT were significantly decreased(P <0.05).However,the essential oil of Cinnamomum camphora leaf had no significant effect on IL-4(P>0.05). Pathological examination showed a large number of inflammatory cells in the model group. Inflammatory cells were not seen in the high and low doses of essential oil group.The dermis slightly thickened in the low doses of essential oil group. In the 2,4-dinitrofluorobenzene model of allergic contact dermatitis,compared with the model group the contents of LTB4 was significantly decreased(P<0.05). However,the essential oil of Cinnamomum camphora leaves had no significant effect on IL-4(P>0.05). [Conclusion] Essential oil of Cinnamomum camphora leaves has a significant antipruritic effect. Its mechanism is to reduce the contents of 5-HT and LTB4.
[Objective] To study the antipruritic effect of essential oil of Cinnamomum camphora leaves and its possible mechanism.[Methods] The model of pruritus induced by 4-aminopyridine in mice and the model of allergic contact dermatitis induced by 2,4-dinitrofluorobenzene in mice were explored. The antipruritic effect of essentia oil of Cinnamomum camphora leaves was investigated.[Results] In 4-aminopyridine pruritus model,compared with the model group the number of licking body in high and low doses of essential oil group was significantly decreased in 10 min(P<0.01). The contents of LTB4 and 5-HT were significantly decreased(P <0.05).However,the essential oil of Cinnamomum camphora leaf had no significant effect on IL-4(P>0.05). Pathological examination showed a large number of inflammatory cells in the model group. Inflammatory cells were not seen in the high and low doses of essential oil group.The dermis slightly thickened in the low doses of essential oil group. In the 2,4-dinitrofluorobenzene model of allergic contact dermatitis,compared with the model group the contents of LTB4 was significantly decreased(P<0.05). However,the essential oil of Cinnamomum camphora leaves had no significant effect on IL-4(P>0.05). [Conclusion] Essential oil of Cinnamomum camphora leaves has a significant antipruritic effect. Its mechanism is to reduce the contents of 5-HT and LTB4.
引文
[1]付宇新,江香梅,罗丽萍,等.不同化学类型樟树叶挥发油成分的GC-MS分析[J].林业工程学报,2016,1(2):72-76.
[2]张谨楠,李志优,王小青,等.南昌樟叶挥发油化学成分分析及对金黄色葡萄球菌和大肠杆菌抑菌杀菌研究[J].时珍国医国药,2014,25(10):2381-2384.
[3]袁琼,全沁果,李婷,等.超声波辅助水提香樟叶中的抑菌成分[J].中国食品添加剂,2017,8:102-105.
[4]王进,曹先爽,宋丽,等.吹扫捕集-热脱附-气相色谱-质谱联用法分析不同产地香樟叶精油成分及抑菌活性比较[J].食品科学,2017,38(12):131-136.
[5]中华本草编委会.中华本草(第3册)[M].上海:上海科学技术出版社,1999:29-34.
[6]张莉莉,王洁,江涛,等.香鳞毛蕨有效部位的止痒和抗过敏作用[J].中成药,2016,38(16):2476-2480.
[7]莫秀梅,刘俊峰,陈玉新,等.培土清心颗粒对豚鼠皮肤瘙痒模型的止痒效果研究[J].新中医,2015,47(7):272-274.
[8]王晖,沈非沉,陈垦,等.薄荷醇的止痒作用及对瘙痒相关因子的影响[J].中草药,2009,40(10):1617-1619.
[9]孙占学.凉血祛湿止痒汤对小鼠特应性皮炎模型血清IL-2,IL-4及皮损白三烯B4的影响[J].中国皮肤性病学杂志,2016,30(11):1169-1171.
[10]Slander S,Steinhoff M,Schmelze M,et al.Neurophysidogy of pruritus:Cutaneous elicitation of itch[J].Arch Dermatol,2003.139:1463-1470.
[11]丁媛,普雄明.瘙痒的发生机制、相关疾病和治疗[J].中国麻风皮肤病杂志,2006,6(22):492-495.
[12]Berendsen HH,Broekkamp CL.A peripheral 5-HT1D-like receptor involved in serotonergic induced hindlimb scratching in rats[J].Eur J Pharmacol,1991,194:201-208.
[13]Bolognial JL,Jorizzo JL,Rapini RP.皮肤病学[M].第2版.北京:北京大学医学出版社,2011:246.
[14]杨慧敏,徐佳,杨岚,等.皮肤瘙痒的发生机制与中医辨证施治相关性探讨[J].中国中西医结合皮肤性病学杂志,2006,5(3):175-181.
[15]吴秋菊,马鹏程,陈志强.瘙痒介质的研究进展[J].国外医学皮肤病学分册,2002,28(11):25-28.
[2]张谨楠,李志优,王小青,等.南昌樟叶挥发油化学成分分析及对金黄色葡萄球菌和大肠杆菌抑菌杀菌研究[J].时珍国医国药,2014,25(10):2381-2384.
[3]袁琼,全沁果,李婷,等.超声波辅助水提香樟叶中的抑菌成分[J].中国食品添加剂,2017,8:102-105.
[4]王进,曹先爽,宋丽,等.吹扫捕集-热脱附-气相色谱-质谱联用法分析不同产地香樟叶精油成分及抑菌活性比较[J].食品科学,2017,38(12):131-136.
[5]中华本草编委会.中华本草(第3册)[M].上海:上海科学技术出版社,1999:29-34.
[6]张莉莉,王洁,江涛,等.香鳞毛蕨有效部位的止痒和抗过敏作用[J].中成药,2016,38(16):2476-2480.
[7]莫秀梅,刘俊峰,陈玉新,等.培土清心颗粒对豚鼠皮肤瘙痒模型的止痒效果研究[J].新中医,2015,47(7):272-274.
[8]王晖,沈非沉,陈垦,等.薄荷醇的止痒作用及对瘙痒相关因子的影响[J].中草药,2009,40(10):1617-1619.
[9]孙占学.凉血祛湿止痒汤对小鼠特应性皮炎模型血清IL-2,IL-4及皮损白三烯B4的影响[J].中国皮肤性病学杂志,2016,30(11):1169-1171.
[10]Slander S,Steinhoff M,Schmelze M,et al.Neurophysidogy of pruritus:Cutaneous elicitation of itch[J].Arch Dermatol,2003.139:1463-1470.
[11]丁媛,普雄明.瘙痒的发生机制、相关疾病和治疗[J].中国麻风皮肤病杂志,2006,6(22):492-495.
[12]Berendsen HH,Broekkamp CL.A peripheral 5-HT1D-like receptor involved in serotonergic induced hindlimb scratching in rats[J].Eur J Pharmacol,1991,194:201-208.
[13]Bolognial JL,Jorizzo JL,Rapini RP.皮肤病学[M].第2版.北京:北京大学医学出版社,2011:246.
[14]杨慧敏,徐佳,杨岚,等.皮肤瘙痒的发生机制与中医辨证施治相关性探讨[J].中国中西医结合皮肤性病学杂志,2006,5(3):175-181.
[15]吴秋菊,马鹏程,陈志强.瘙痒介质的研究进展[J].国外医学皮肤病学分册,2002,28(11):25-28.