基于HPLC-ABTS-DAD-Q-TOF/MS的黄芩抗氧化活性成分研究
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摘要
目的建立黄芩特征化学成分和抗氧化活性相关联的二维指纹图谱,研究黄芩抗氧化活性物质。方法利用高效液相多检测器联用在线检测体系,对黄芩中化学成分进行检测,共鉴定出抗氧化活性的成分10种;然后采用清除效率为指标对各活性成分的抗氧化活性进行评价。结果黄芩苷、5,7,2′,5′-四羟基-8,6′-二甲氧基黄酮、5,7,2′-三羟基-6-甲氧基黄酮、黄芩素等化合物具有较强的清除自由基的活性;6-C-阿拉伯糖基-8-C-葡萄糖基白杨素、6-C-葡萄糖基-8-C-阿拉伯糖基白杨素、千层纸素苷、白杨素-7-O-葡萄糖醛酸苷、汉黄芩苷、去甲汉黄芩素等化合物清除自由基的活性次之。结论采用HPLC-ABTS-DADQ-TOF/MS对黄芩中的抗氧化活性成分进行快速分析鉴定,为黄芩的抗氧化物质基础研究提供数据支持。
Objective To establish a two-dimensional fingerprint of Scutellaria baicalensis between characteristic chemical composition and anti-oxidant activity, and to study its anti-oxidant constituents on-line. Methods The scavenging activities of S.baicalensis for ABTS+ by HPLC-ABTS-DAD-Q-TOF/MS was studied and its active constituents were screened. A total of ten anti-oxidant components in S. baicalensis were identified. The anti-oxidant activities of different active ingredients were evaluated by anti-oxidant efficiency. Results The data showed that baicalin, 5,7,2′,5′-tetrahydroxy-8,6′-dimethoxy flavone, 5,7,2′-trihydroxy-6-methoxyflavone, and baicalein had stronger anti-oxidant activity, and 6-C-arabinosyl-8-C-glucosylchrysin, 6-C-glucosyl-8-C-arabinosyl-chrysin, oroxyloside, chrysin-7-O-glucuronide, wogonoside, and norwogonin had less anti-oxidant activity. Conclusion HPLC-ABTS-DAD-Q-TOF/MS method can rapidly analyze and identify the anti-oxidant components in S.baicalensis, and these experimental data can provide support for the basic research of the anti-oxidant ingredients of S. baicalensis.
Objective To establish a two-dimensional fingerprint of Scutellaria baicalensis between characteristic chemical composition and anti-oxidant activity, and to study its anti-oxidant constituents on-line. Methods The scavenging activities of S.baicalensis for ABTS+ by HPLC-ABTS-DAD-Q-TOF/MS was studied and its active constituents were screened. A total of ten anti-oxidant components in S. baicalensis were identified. The anti-oxidant activities of different active ingredients were evaluated by anti-oxidant efficiency. Results The data showed that baicalin, 5,7,2′,5′-tetrahydroxy-8,6′-dimethoxy flavone, 5,7,2′-trihydroxy-6-methoxyflavone, and baicalein had stronger anti-oxidant activity, and 6-C-arabinosyl-8-C-glucosylchrysin, 6-C-glucosyl-8-C-arabinosyl-chrysin, oroxyloside, chrysin-7-O-glucuronide, wogonoside, and norwogonin had less anti-oxidant activity. Conclusion HPLC-ABTS-DAD-Q-TOF/MS method can rapidly analyze and identify the anti-oxidant components in S.baicalensis, and these experimental data can provide support for the basic research of the anti-oxidant ingredients of S. baicalensis.
引文
[1]中国药典[S].一部.2015.
[2]郭少英,程发峰,钟相根,等.黄芩苷的体外抗氧化研究[J].时珍国医国药,2011,22(1):9-11.
[3]苏宁,罗荣敬,苏杭,等.黄芩苷对糖尿病肾病大鼠肾功能及其抗氧化应激作用的研究[J].中药新药与临床药理,2007,9(18):341-345.
[4]Han J,Ye M,Xu M,et al.Characterization of flavonoids in the traditional Chinese herbal medicine-Huangqin by liquid chromatography coupled with electrospray ionization mass spectrometry[J].J Chromatogr B,2007,848(2):355-362.
[5]杨培树,张娜,郝文立.电喷雾串联质谱法鉴定黄芩药材的化学成分[J].中药材,2017,40(2):369-372.
[6]张婷,美尔哈巴·热西提,林潇,等.HPLC-MS/MS法测定银黄颗粒中绿原酸和黄芩苷[J].中草药,2012,43(4):711-713.
[7]刘大伟,闫广利,方圆,等UPLC-ESI-TOF/MS应用于黄芩化学成分的快速分析[J].中医药信息,2012,29(4):20-24.
[8]Wang R,Luo J G,Kong L Y.Screening of radical scavengers in Scutellaria baicalensis using HPLC with diode array and chemiluminescence detection[J].J Sep Sci,2012,35:2223-2227
[9]郭继芬,陈四平,乔善义,等.黄芩总黄酮提取物的HPLC-MS/MS分析[J].药物分析杂志,2005,25(3):267-269.
[10]Ablajan K,Zeper A,Shang X Y,et al.Stuctural characterization of flavonol 3,7-di-O-glycosides and determination of the glycosylation position by using negative ion electrospray ionization tandem mass spectrotrometry[J].J Mass Spectrom,2006,41(3):352-360.
[11]魏朝良.黄酮类化合物及清除自由基机制的探讨[J].中成药,2005,27(2):239-241.
[12]曹志超,顾翔,苏佩清.黄酮类化合物抗氧化及其作用机制的研究进展[J].实用临床医药杂志,2009,13(7):110-112.
[13]鲁晓翔.黄酮类化合物抗氧化作用机制研究进展[J].食品研究与开发,2012,33(3):220-224.
[14]刘杰,王伯初,彭亮,等.黄酮类抗氧化剂的构-效关系[J].重庆大学学报,2004,27(2):120-124.
[15]郑勇凤,王佳婧,傅超美,等.黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.
[2]郭少英,程发峰,钟相根,等.黄芩苷的体外抗氧化研究[J].时珍国医国药,2011,22(1):9-11.
[3]苏宁,罗荣敬,苏杭,等.黄芩苷对糖尿病肾病大鼠肾功能及其抗氧化应激作用的研究[J].中药新药与临床药理,2007,9(18):341-345.
[4]Han J,Ye M,Xu M,et al.Characterization of flavonoids in the traditional Chinese herbal medicine-Huangqin by liquid chromatography coupled with electrospray ionization mass spectrometry[J].J Chromatogr B,2007,848(2):355-362.
[5]杨培树,张娜,郝文立.电喷雾串联质谱法鉴定黄芩药材的化学成分[J].中药材,2017,40(2):369-372.
[6]张婷,美尔哈巴·热西提,林潇,等.HPLC-MS/MS法测定银黄颗粒中绿原酸和黄芩苷[J].中草药,2012,43(4):711-713.
[7]刘大伟,闫广利,方圆,等UPLC-ESI-TOF/MS应用于黄芩化学成分的快速分析[J].中医药信息,2012,29(4):20-24.
[8]Wang R,Luo J G,Kong L Y.Screening of radical scavengers in Scutellaria baicalensis using HPLC with diode array and chemiluminescence detection[J].J Sep Sci,2012,35:2223-2227
[9]郭继芬,陈四平,乔善义,等.黄芩总黄酮提取物的HPLC-MS/MS分析[J].药物分析杂志,2005,25(3):267-269.
[10]Ablajan K,Zeper A,Shang X Y,et al.Stuctural characterization of flavonol 3,7-di-O-glycosides and determination of the glycosylation position by using negative ion electrospray ionization tandem mass spectrotrometry[J].J Mass Spectrom,2006,41(3):352-360.
[11]魏朝良.黄酮类化合物及清除自由基机制的探讨[J].中成药,2005,27(2):239-241.
[12]曹志超,顾翔,苏佩清.黄酮类化合物抗氧化及其作用机制的研究进展[J].实用临床医药杂志,2009,13(7):110-112.
[13]鲁晓翔.黄酮类化合物抗氧化作用机制研究进展[J].食品研究与开发,2012,33(3):220-224.
[14]刘杰,王伯初,彭亮,等.黄酮类抗氧化剂的构-效关系[J].重庆大学学报,2004,27(2):120-124.
[15]郑勇凤,王佳婧,傅超美,等.黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.