基于PCR-RFLP技术分析H-FABP基因在5个猪群体中的多态性
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摘要
【目的】肉质是猪肉的重要经济指标,肌内脂肪(IMF)含量是影响肉质的重要因素。H-FABP基因是调控猪肉IMF含量的候选基因。【方法】本实验采用PCR-RFLP技术分析H-FABP基因多态性,并检测该基因在5个猪群体:杜洛克(100头)、长白(80头)、大白(150头)、申农(80头)、梅山(140头)的基因型和基因频率。【结果】H-FABP基因在杜洛克、大白、申农猪群体中检测出DD、Dd和dd 3种基因型。在杜洛克群体中的基因型频率:DD(0.02)、Dd(0.23)和dd(0.75);在大白猪群体中基因型频率DD∶(0.25)、Dd(0.49)和dd(0.26);在申农猪群体中基因型频率:DD(0.10)、Dd(0.30)和dd(0.60)。在长白猪群体中检测的所有样品为dd型;在梅山猪群体中检测的所有样品为DD型。【结论】H-FABP基因在5个猪群体中呈现不同基因型。在长白猪和梅山猪群体中呈现偏态现象。在杜洛克和申农猪群体中主要为dd型,在大白群体中主要为Dd型。
【Objective】Meat quality is one of the important indicators of pork. Intramuscular fat content( IMF) is one of the important factors in affecting meat quality. H-FABP gene is a candidate gene as regulating pig intramuscular fat traits. 【Method】The experiment based on PCR-RFLP was conducted,and H-FABP gene polymorphisms in five pig groups: Duroc( 100),Landrace( 80),Large White( 150),Shennong( 80) and Meishan( 140) were detected. 【Result】H-FABP genes in Duroc,Large White and Shennong groups had DD,Dd and dd genotype. The genotype frequencies in Duroc groups were DD( 0. 02),Dd( 0. 23) and dd( 0. 75),the genotype frequencies in Large White groups were DD( 0. 25),Dd( 0. 49) and dd( 0. 26),and the genotype frequencies in Shennong groups were DD( 0. 10),Dd( 0. 30)and dd( 0. 60). The genotype frequencies in Landrace groups were dd( 1. 0),no Dd and DD; Genotype frequencies in Meishan groups: DD( 1. 0). 【Conclusion】H-FABP gene present different genotypes in five pig groups. Only gentype was detected in Landrace and Meishan populations. dd genotype was main genotype in Duroc and shennong groups,Dd genotype was main genotype in Large White groups.
【Objective】Meat quality is one of the important indicators of pork. Intramuscular fat content( IMF) is one of the important factors in affecting meat quality. H-FABP gene is a candidate gene as regulating pig intramuscular fat traits. 【Method】The experiment based on PCR-RFLP was conducted,and H-FABP gene polymorphisms in five pig groups: Duroc( 100),Landrace( 80),Large White( 150),Shennong( 80) and Meishan( 140) were detected. 【Result】H-FABP genes in Duroc,Large White and Shennong groups had DD,Dd and dd genotype. The genotype frequencies in Duroc groups were DD( 0. 02),Dd( 0. 23) and dd( 0. 75),the genotype frequencies in Large White groups were DD( 0. 25),Dd( 0. 49) and dd( 0. 26),and the genotype frequencies in Shennong groups were DD( 0. 10),Dd( 0. 30)and dd( 0. 60). The genotype frequencies in Landrace groups were dd( 1. 0),no Dd and DD; Genotype frequencies in Meishan groups: DD( 1. 0). 【Conclusion】H-FABP gene present different genotypes in five pig groups. Only gentype was detected in Landrace and Meishan populations. dd genotype was main genotype in Duroc and shennong groups,Dd genotype was main genotype in Large White groups.
引文
[1]Zimmerman A W,Veerkamp J H.Members of the fatty acid-binding protein family inhibit cell-free protein synthesis[J].FEBS Lett,1998,437(3):183-186.
[2]Gerbens F,Verburg F J,Van Moerkerk H T,et al.Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs[J].J Anim Sci,2001,79(2):347-354.
[3]Gerbens F,van Erp A J,Harders F L,et al.Effect of genetic variants of the heart fatty acid binding protein gene on intramuscular fat and performance traits in pigs[J].J Anim Sci,1999,77(4):846-852.
[4]Chen J N,Jiang Y Z,Cen W M,et al.Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations[J].Genet Mol Res,2014,13(3):6759-6772.
[5]Nechtelberger D,Pires V,S9olknet J,et al.Intramuscular fat content and genetic variants at fatty acid-binding protein loci in Austrian pigs[J].J Anim Sci,2001,79(11):2798-2804.
[6]林万华,任军,丁能水,等.H-FABP基因型对二花脸猪相关性状影响的初步分析[J].畜牧兽医学报,2003,34(4):318-324.
[7]OVilo C,Oliver A,Noguera J L,et al.Test for positional candidate genes for body composition on pig chromosome6[J].Genet Sel Evol,2002,34(4):465-479.
[8]Gerbens F,de Koning D J,Harders F L,et al.The effect of adipocyte and heart fatty acid binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs[J].J Anim Sci,2000,78(3):552-559.
[9]林万华,黄路生,任军,等.中外十个猪种H-FABP基因遗传变异的研究[J].遗传学报,2002,29(1):12-15.
[10]许卫华,康丽珠,林秀娇,等.槐猪H-FABP基因多态性及其与肌内脂肪含量相关性研究[J].福建师范大学学报(自然科学版),2014,30(2):76-80.
[11]彭先文,梅书棋,李良华,等.湖北白猪及其杂交后代H-FABP基因型与相关性状关系研究[J].中国农业科学,2008,41(2):570-575.
[12]李志娟,高士争,潘洪彬,等.不同H-FABP基因型滇南小耳猪脂类合成代谢相关基因表达水平研究[J].中国细胞生物学学报,2013,35(5):661-667.
[13]庞卫军,白亮,杨公社.西部地区主要猪种H-FABP基因多态性、IMF含量及不同基因型脂肪细胞脂滴量的关系[J].遗传学报,2006,33(6):515-524.
[14]Chao Z,Wang F,Deng C Y,et al.Distribution and linkage disequilibrium analysis of polymorphisms of MC4R,LEP,H-FABP genes in the different populations of pigs,associated with economic traits in DIV2 line[J].Mol Biol Rep,2012,39(5):6329-6335.
[15]Brocks L,Hulsegge B,Merkus G.Histochemical characteristics in relation to meat quality properties in the longissimus lumborum of fast and lean growing lines of large white pigs[J].Meat Sci,1998,50(4):411-420.
[2]Gerbens F,Verburg F J,Van Moerkerk H T,et al.Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs[J].J Anim Sci,2001,79(2):347-354.
[3]Gerbens F,van Erp A J,Harders F L,et al.Effect of genetic variants of the heart fatty acid binding protein gene on intramuscular fat and performance traits in pigs[J].J Anim Sci,1999,77(4):846-852.
[4]Chen J N,Jiang Y Z,Cen W M,et al.Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations[J].Genet Mol Res,2014,13(3):6759-6772.
[5]Nechtelberger D,Pires V,S9olknet J,et al.Intramuscular fat content and genetic variants at fatty acid-binding protein loci in Austrian pigs[J].J Anim Sci,2001,79(11):2798-2804.
[6]林万华,任军,丁能水,等.H-FABP基因型对二花脸猪相关性状影响的初步分析[J].畜牧兽医学报,2003,34(4):318-324.
[7]OVilo C,Oliver A,Noguera J L,et al.Test for positional candidate genes for body composition on pig chromosome6[J].Genet Sel Evol,2002,34(4):465-479.
[8]Gerbens F,de Koning D J,Harders F L,et al.The effect of adipocyte and heart fatty acid binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs[J].J Anim Sci,2000,78(3):552-559.
[9]林万华,黄路生,任军,等.中外十个猪种H-FABP基因遗传变异的研究[J].遗传学报,2002,29(1):12-15.
[10]许卫华,康丽珠,林秀娇,等.槐猪H-FABP基因多态性及其与肌内脂肪含量相关性研究[J].福建师范大学学报(自然科学版),2014,30(2):76-80.
[11]彭先文,梅书棋,李良华,等.湖北白猪及其杂交后代H-FABP基因型与相关性状关系研究[J].中国农业科学,2008,41(2):570-575.
[12]李志娟,高士争,潘洪彬,等.不同H-FABP基因型滇南小耳猪脂类合成代谢相关基因表达水平研究[J].中国细胞生物学学报,2013,35(5):661-667.
[13]庞卫军,白亮,杨公社.西部地区主要猪种H-FABP基因多态性、IMF含量及不同基因型脂肪细胞脂滴量的关系[J].遗传学报,2006,33(6):515-524.
[14]Chao Z,Wang F,Deng C Y,et al.Distribution and linkage disequilibrium analysis of polymorphisms of MC4R,LEP,H-FABP genes in the different populations of pigs,associated with economic traits in DIV2 line[J].Mol Biol Rep,2012,39(5):6329-6335.
[15]Brocks L,Hulsegge B,Merkus G.Histochemical characteristics in relation to meat quality properties in the longissimus lumborum of fast and lean growing lines of large white pigs[J].Meat Sci,1998,50(4):411-420.