低温胁迫对五种景天科多肉植物生理指标的影响
|
摘要
以观音莲(Herba monachosori Henryi)、姬星美人(Sedum dasyphyllum)、奥普琳娜(Graptoveria ‘Opalina’)、白美人(Pachyphytum oviferum)和虹之玉(Sedum rubrotinctum)5种景天科多肉植物3年生扦插苗为试材,以人工低温胁迫的方法,通过对胁迫后植物叶片可溶性糖含量、叶片相对含水量、脯氨酸含量、丙二醛含量、恢复生长后植物成活率的测定,研究了低温对5种多肉植物的生理影响,以期为筛选耐低温多肉植物品种提供参考依据。结果表明:随着低温胁迫的加剧,5种多肉植物体内脯氨酸含量、叶片相对含水量、可溶性糖含量、丙二醛含量在总体上均呈上升趋势,但增幅略有不同。奥普琳娜的叶片相对含水量和丙二醛含量变化最为明显,观音莲最不明显。在低温胁迫后,5种多肉植物中,观音莲的有机溶质脯氨酸和可溶性糖含量最高,奥普琳娜最低。综合分析得出5种多肉植物的耐寒性从强到弱依次为观音莲>姬星美人>虹之玉>白美人>奥普琳娜。
Five species of asexual succulent plants including Herba monachosori Henryi,Sedum dasyphyllum,Graptoveria ‘Opalina',Pachyphytum oviferumand Sedum rubrotinctum,were used as test materials to study their cold resistance by artificial low-temperature stress method,which aimed to provide certain basis for low temperature resistant species selection.Physiological indexes such as soluble sugar,leaf relative water content,proline and malondialdehyde under low temperature stress were measured.And the survival rate after low temperature stress were determined.The results showed that the proline content,leaf relative water content,soluble sugar content,and malondialdehyde content in five kinds of succulents showed an upward trend with increasing cold stress,but the increase was slightly different.The changes in leaf relative water content and malondialdehyde content were the most evident in Graptoveria ‘Opalina',and the lowest was in Herba monachosori Henryi.Under low temperature stress,the organic solute of Herba monachosori Henryi had the highest content of proline and soluble sugar,and Graptoveria ‘Opalina'was the lowest.The cold resistance of the five succulents was ranked in the following order:Herba monachosori Henryi>Sedum dasyphyllum >Sedum rubrotinctum > Pachyphytum oviferum >Graptoveria ‘Opalina'.
Five species of asexual succulent plants including Herba monachosori Henryi,Sedum dasyphyllum,Graptoveria ‘Opalina',Pachyphytum oviferumand Sedum rubrotinctum,were used as test materials to study their cold resistance by artificial low-temperature stress method,which aimed to provide certain basis for low temperature resistant species selection.Physiological indexes such as soluble sugar,leaf relative water content,proline and malondialdehyde under low temperature stress were measured.And the survival rate after low temperature stress were determined.The results showed that the proline content,leaf relative water content,soluble sugar content,and malondialdehyde content in five kinds of succulents showed an upward trend with increasing cold stress,but the increase was slightly different.The changes in leaf relative water content and malondialdehyde content were the most evident in Graptoveria ‘Opalina',and the lowest was in Herba monachosori Henryi.Under low temperature stress,the organic solute of Herba monachosori Henryi had the highest content of proline and soluble sugar,and Graptoveria ‘Opalina'was the lowest.The cold resistance of the five succulents was ranked in the following order:Herba monachosori Henryi>Sedum dasyphyllum >Sedum rubrotinctum > Pachyphytum oviferum >Graptoveria ‘Opalina'.
引文
[1]唐文秀,黄仕训,盘波,等.桂林植物园多肉植物的引种栽培试验研究[J].北方园艺,2009(1):189-192.
[2]轩华强,朱方平,张伟.多肉植物雪国万象叶片诱导组培苗生产体系的建立[J].中国种业,2018(8):60-63.
[3]周慧妍,刘雪莹,李雅博,等.9种百合科十二卷属多肉植物启动诱导的研究[J].北京农学院学报,2018(4):1-3.
[4]柴清东,陈明真,范之斌,等.多肉植物黑王子组织培养体系的研究[J].曲阜师范大学学报(自然科学版),2018,44(1):91-94.
[5]陈云.多肉植物在园林栽培中的应用探讨[J].花卉,2018(12):134.
[6]吴云帆,腾玥.多肉植物在商务办公环境中降辐射及增湿作用的研究[J].上海农业科技,2014(4):6-8.
[7]宋正达,朱洪武,陈梅香.龙舌兰属三种多肉植物的耐寒性试验[J].黑龙江农业科学,2014(11):174.
[8]兑宝峰.多肉植物冬季管理[J].中国花卉园艺,2013(22):36-38.
[9]郑春.多肉植物的园林应用研究[J].福建建筑,2014(7):24-27.
[10]杨桂英,何瀚,曹子林,等.3种不同倍性滇山茶的耐寒性研究[J].云南大学学报(自然科学版),2016(2):335-343.
[11]陈雅君,冯淑华,陈桂芬.植物抗旱性鉴定指标的研究现状与进展[J].中国林副特产,2005(6):62-63.
[12]叶艳然,王文莉,郑成淑,等.四种野生苔草属植物的耐寒性评价[J].应用生态学报,2017(1):89-95.
[13]王腾飞,裴玉贺,郭新梅,等.3个玉米品种苗期耐寒性鉴定[J].核农学报,2017(4):803-808.
[14]陈少瑜,郎南军,李吉跃,等.干旱胁迫下3树种苗木叶片相对含水量、质膜相对透性和脯氨酸含量的变化[J].西部林业科学,2004(3):30-33,41.
[15]杨希,隆学武,曾华浩,等.美丽针葵耐寒性试验[J].福建林业科技,2002(2):56-57.
[16]LEILA H,REZA M A.Physio-biochemical and proteome analysis of chickpea in early phases of cold stress[J].Journal of Plant Physiology,2013(6):86-97.
[17]崔国文,马春平.紫花苜蓿叶片形态结构及其与抗寒性的关系[J].草地学报,2007,15(1):70-75.
[18]陈刚,杨玉珍,彭方仁,等.低温胁迫下不同种源香椿含水量和渗透调节物质含量差异及其与抗寒性的相关性[J].植物资源与环境学报,2014(4):47-54.
[2]轩华强,朱方平,张伟.多肉植物雪国万象叶片诱导组培苗生产体系的建立[J].中国种业,2018(8):60-63.
[3]周慧妍,刘雪莹,李雅博,等.9种百合科十二卷属多肉植物启动诱导的研究[J].北京农学院学报,2018(4):1-3.
[4]柴清东,陈明真,范之斌,等.多肉植物黑王子组织培养体系的研究[J].曲阜师范大学学报(自然科学版),2018,44(1):91-94.
[5]陈云.多肉植物在园林栽培中的应用探讨[J].花卉,2018(12):134.
[6]吴云帆,腾玥.多肉植物在商务办公环境中降辐射及增湿作用的研究[J].上海农业科技,2014(4):6-8.
[7]宋正达,朱洪武,陈梅香.龙舌兰属三种多肉植物的耐寒性试验[J].黑龙江农业科学,2014(11):174.
[8]兑宝峰.多肉植物冬季管理[J].中国花卉园艺,2013(22):36-38.
[9]郑春.多肉植物的园林应用研究[J].福建建筑,2014(7):24-27.
[10]杨桂英,何瀚,曹子林,等.3种不同倍性滇山茶的耐寒性研究[J].云南大学学报(自然科学版),2016(2):335-343.
[11]陈雅君,冯淑华,陈桂芬.植物抗旱性鉴定指标的研究现状与进展[J].中国林副特产,2005(6):62-63.
[12]叶艳然,王文莉,郑成淑,等.四种野生苔草属植物的耐寒性评价[J].应用生态学报,2017(1):89-95.
[13]王腾飞,裴玉贺,郭新梅,等.3个玉米品种苗期耐寒性鉴定[J].核农学报,2017(4):803-808.
[14]陈少瑜,郎南军,李吉跃,等.干旱胁迫下3树种苗木叶片相对含水量、质膜相对透性和脯氨酸含量的变化[J].西部林业科学,2004(3):30-33,41.
[15]杨希,隆学武,曾华浩,等.美丽针葵耐寒性试验[J].福建林业科技,2002(2):56-57.
[16]LEILA H,REZA M A.Physio-biochemical and proteome analysis of chickpea in early phases of cold stress[J].Journal of Plant Physiology,2013(6):86-97.
[17]崔国文,马春平.紫花苜蓿叶片形态结构及其与抗寒性的关系[J].草地学报,2007,15(1):70-75.
[18]陈刚,杨玉珍,彭方仁,等.低温胁迫下不同种源香椿含水量和渗透调节物质含量差异及其与抗寒性的相关性[J].植物资源与环境学报,2014(4):47-54.