甜瓜细菌性果斑病抗性基因连锁的SSR分子标记
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摘要
为研究甜瓜抗果斑病资源辽宁神帅的抗性基因遗传规律和紧密连锁的简单重复序列(simple sequence repeat,简称SSR)分子标记,以甜瓜抗病品种辽宁神帅和感病品种皮山奎瑞克的杂交F2分离群体作为试验材料,采用集群分离分析法(bulked segregation analysis,简称BSA)和SSR分子标记辅助选择的方法对甜瓜抗果斑病基因初步定位。结果表明,从288对SSR引物组合中筛选出1对与抗性基因连锁的标记BCM184,遗传距离为12.4 c M,最终将抗性基因初步定位到连锁群(linkage group,简称LG)Ⅳ上。
引文
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[28]王士磊,丁正权,黄海祥.水稻隐性早熟突变体ref早熟性的遗传分析和基因定位[J].江苏农业学报,2016,32(4):721-724.
[29]安百伟,赵亮,狄佳春,等.陆地棉Bt抗虫基因类型鉴定与染色体定位[J].江苏农业学报,2016,32(2):262-266.
[30]Parida S K,Kalia S S,Dalal V,et al.Informative genomic microsatellite markers for efficient genotyping applications in sugarcane[J].Theoretical and Applied Genetics,2009,118(2):327-338.
[31]卢浩,王贤磊,高兴旺,等.甜瓜‘PMR6’抗白粉病基因的遗传及其定位研究[J].园艺学报,2015,42(6):1121-1128.
[2]胡国智,冯炯鑫,张炎,等.施肥对甜瓜生长、养分吸收及产量品质的影响[J].新疆农业科学,2014,51(8):1445-1450.
[3]Crall J M,Schenck N C.Bacterial fruit rot of watermelon in Florida[J].Plant Disease Reporter,1969,53(1):74-75.
[4]Bahar O,Burdman S.Bacterial fruit blotch:a threat to the cucurbit industry[J].Israel Journal of Plant Sciences,2010,58(1):19-31.
[5]Wechter W P,Levi A,Ling K S,et al.Identification of resistance to Acidovorax avenae subsp.citrulli among melon(Cucumis spp.)plant introductions[J].Hortscience,2011,46(2):207-212.
[6]Maynard D N,Hopkins D L.Watermelon fruit disorders[J].Horttechnology,1999,9(2):155-161.
[7]李俊阁.甜瓜细菌性果斑病抗性鉴定及抗性相关生理研究[D].乌鲁木齐:新疆农业大学,2015.
[8]Schaad N W,Postnikova E,Sechler A,et al.Reclassification of subspecies of Acidovorax avenae as A.avenae(Manns 1905)emend.,A.cattleyae(Pavarino,1911)comb.nov.,A.citrulli Schaad et al.,1978)comb.nov.,and proposal of A.oryzae sp.nov.[J].Systematic and Applied Microbiology,2008,31(6/8):434-446.
[9]Willems A,Goor M,Thielemans S,et al.Transfer of several phytopathogenic Pseudomonas species to Acidovorax as Acidovorax avenae subsp.avenae subsp.nov.,comb.nov.,Acidovorax avenae subsp.citrulli,Acidovorax avenae subsp.cattleyae,and Acidovorax konjaci[J].International Journal of Systematic Bacteriology,1992,42(42):107-119.
[10]赵廷昌,孙福在,王兵万,等.哈密瓜细菌性果斑病病原菌鉴定[J].植物病理学报,2001,31(4):357-364.
[11]翟艳霞,胡俊,黄俊霞,等.哈密瓜叶片结构与细菌性果斑病抗性的研究[J].内蒙古农业大学学报(自然科学版),2006,27(1):47-50.
[12]Hopkins D L,Thompson C M.Seed transmission of Acidovorax avenae subsp.citrulli in cucurbits[J].Hort Science:a Publication of the American Society for Horticultural Science,2002,37(6):924-926.
[13]阎莎莎,王铁霖,赵廷昌.瓜类细菌性果斑病研究进展[J].植物检疫,2011,25(3):71-76.
[14]宁雪飞,高兴旺,李冠.甜瓜抗白粉病分子育种研究进展[J].北方园艺,2013(2):180-184.
[15]Kuzuya M,Hosoya K,Yashiro K,et al.Powdery mildew(Sphaerotheca fuliginea)resistance in melon is selectable at the haploid level[J].Journal of Experimental Botany,2003,54(384):1069-1074.
[16]Garcia-Mas J,Benjak A,Sanseverino W A,et al.The genome of melon(Cucumis melo L.)[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(29):11872-11877.
[17]李俊阁,王惠林,张亮,等.不同甜瓜材料苗期对细菌性果斑病抗病性鉴定[J].新疆农业科学,2015,52(10):1843-1848.
[18]黄俊霞.内蒙古地区哈密瓜细菌性果斑病发病规律的初步研究[D].北京:中国农业大学,2006.
[19]Porebski S,Bailey L G,Baum B R.Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components[J].Plant Molecular Biology Reporter,1997,15(1):8-15.
[20]Mirbabaee S A,Mardi M,Mahmoodi P,et al.Development of new microsatellite markers from an enriched genomic library of date palm(Phoenix dactylifera L.)[J].Journal of Pomology&Horticultural Science,2011,86(5):539-541.
[21]Gonzalo M J,Oliver M,Garcia-Mas J,et al.Simple-sequence repeat markers used in merging linkage maps of melon(Cucumis melo L.)[J].Theoretical and Applied Genetics,2005,110(5):802-811.
[22]张微微,潘俊松,蒋苏,等.黄瓜RIL群体侧枝相关性状QTL定位[J].江苏农业学报,2017,33(1):174-180.
[23]宝华宾,梁帅强,吕远大,等.玉米籽粒蛋白含量Meta-QTL及候选基因分析[J].江苏农业学报,2016,32(4):736-745.
[24]韦志扬.西瓜嫁接苗细菌性果斑病的发生与防治[J].广西热带农业,2007(6):12-13.
[25]Wayne P,Gordon C M,Jim P.Polymorphism revealed by simple sequence repeat[J].Trends in Plant Science,1996,1(7):215-222.
[26]Chistiakov DA,Hellemans B,Volckaert FAM.Microsatellites and their genomic distribution,evolution,function and applications:a review with special reference to fish genetics[J].Aquaculture,2006,255(1/2/3/4):1-29.
[27]周向阳,赵亮,狄佳春,等.抗虫杂交棉苏杂6号抗虫亲本的Bt基因类型鉴定与染色体定位[J].江苏农业学报,2016,32(5):987-991.
[28]王士磊,丁正权,黄海祥.水稻隐性早熟突变体ref早熟性的遗传分析和基因定位[J].江苏农业学报,2016,32(4):721-724.
[29]安百伟,赵亮,狄佳春,等.陆地棉Bt抗虫基因类型鉴定与染色体定位[J].江苏农业学报,2016,32(2):262-266.
[30]Parida S K,Kalia S S,Dalal V,et al.Informative genomic microsatellite markers for efficient genotyping applications in sugarcane[J].Theoretical and Applied Genetics,2009,118(2):327-338.
[31]卢浩,王贤磊,高兴旺,等.甜瓜‘PMR6’抗白粉病基因的遗传及其定位研究[J].园艺学报,2015,42(6):1121-1128.