哈密瓜种质资源苗期细菌性果斑病抗性鉴定
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摘要
【目的】筛选出高抗细菌性果斑病的甜瓜资源,为选育抗病品种奠定基础。【方法】采用室内苗期人工喷雾法接种,采用2种抗性分级方法,以感病品种炮台红为对照,对17份甜瓜资源进行抗性鉴定。【结果】17份供试甜瓜材料中,没有对细菌性果斑病菌免疫的材料,JM17-14的抗性最强,相对抗病指数为0.85,属高抗水平;采用两种抗性分级标准,供试甜瓜材料的抗性强弱顺序基本一致,但抗性水平有差异;17份供试材料中杂交F_1代的抗性多介于其双亲之间,但也有超亲优势存在。【结论】17份供试甜瓜材料中,JM17-14的抗性最强,为高抗细菌性果斑病资源,可用于抗性基因定位及抗病品种选育。
【Objective】 The occurrence of bacterial fruit spot disease of Hami melon in Xinjiang is increasing year by year. This project aims to lay a foundation for the screening of cantaloupe resources with high resistance to bacterial fruit spot disease and the breeding of resistant varieties.【Method】In this study, the susceptible variety "Bataonghong" as control, artificial spray inoculation and two resistance classification methods were used to identify the resistance to Acidovoraxavenae subsp. Citrulli among 17 cantaloupe cultivars at the seedling stage. 【Result】Indoor inoculation test indicated that the resistance of JM17-14 was the strongest among the 17 test materials. The relative disease-resistance index was 0.85, which expressed high resistance level. When using two resistance classification criteria, the resistance order of the tested melon was basically the same, but the resistance level was different, and the resistance of F_1 hybrids in 17 tested materials was mostly between their parents, but there was also overparent dominance.【Conclusion】JM17-14 is the resource of high resistance to bacterial fruit spot disease among the 17 tested melon materials, which can be used for the resistance gene localization and the breeding of disease-resistant varieties. The resistance level of the same melon variety resource is different by using different resistance grading standards. For this purpose, uniform resistance grading standard is required and the results of different investigators are more valuable. The resistance of Hami melon to Acidovoraxavenae subsp. Citrulli may be controlled by multiple genes, and the resistance genetic mechanism needs further study.
【Objective】 The occurrence of bacterial fruit spot disease of Hami melon in Xinjiang is increasing year by year. This project aims to lay a foundation for the screening of cantaloupe resources with high resistance to bacterial fruit spot disease and the breeding of resistant varieties.【Method】In this study, the susceptible variety "Bataonghong" as control, artificial spray inoculation and two resistance classification methods were used to identify the resistance to Acidovoraxavenae subsp. Citrulli among 17 cantaloupe cultivars at the seedling stage. 【Result】Indoor inoculation test indicated that the resistance of JM17-14 was the strongest among the 17 test materials. The relative disease-resistance index was 0.85, which expressed high resistance level. When using two resistance classification criteria, the resistance order of the tested melon was basically the same, but the resistance level was different, and the resistance of F_1 hybrids in 17 tested materials was mostly between their parents, but there was also overparent dominance.【Conclusion】JM17-14 is the resource of high resistance to bacterial fruit spot disease among the 17 tested melon materials, which can be used for the resistance gene localization and the breeding of disease-resistant varieties. The resistance level of the same melon variety resource is different by using different resistance grading standards. For this purpose, uniform resistance grading standard is required and the results of different investigators are more valuable. The resistance of Hami melon to Acidovoraxavenae subsp. Citrulli may be controlled by multiple genes, and the resistance genetic mechanism needs further study.
引文
[1] 王新绘,刘晓颖,李冠.甜瓜细菌性果斑病病原菌的分离鉴定及16S rDNA序列分析[J].食品科学,2015,36(19):186-189.WANG Xin-hui, LIU Xiao-ying, LI Guan. (2015). Isolation, Identification and 16S rDNA Sequence Analysis of Bacterial Fruit Blotch Pathogen in Melon [J]. Food Science, 36(19):186-189. (in Chinese)
[2] 李威,任毓忠,丁建军.新疆瓜类细菌性果斑病品种抗性鉴定[J]. 北方园艺,2007,(3):186-188.LI Wei, REN Yu-zhong, DING Jian-jun. (2007). Identification of Citrull Resistance to Acidovoraxavenae subsp. Citrulli in Xinjiang [J]. Northern Horticulture, (3):186-188. (in Chinese)
[3]王宗伟,汪文静,刘曼双.哈密瓜细菌性果斑病的发生与防治[J].新疆农垦科技,2002,(5):16-17.WANG Zong-wei, WANG Wen-jing, LIU Man-shuang. (2002). The Occurrence and Prevention of Bacterial Fruit Spot Disease of Hami Melon [J]. Xinjiang Agricultural Reclamation Technology,(5):16-17. (in Chinese)
[4]张昕,李国英,任毓忠,等.新疆哈密瓜上两种病原细菌比较鉴定及其田间消长动态的研究[J] .中国农业科学, 2002, 35(7):888-893。ZHANG Xin, LI Guo-ying, REN Yu-zhong, et al. (2002). Identification of Pathogen of Bacterial Disease of Melon and Pathogens' Alternate Predomination Investigation in Field in Xinjiang [J]. Scientia Agricultura Sinica, 35(7):888-893. (in Chinese)
[5]赵廷昌,孙福在,王兵万,等.哈密瓜细菌性果斑病病原鉴定[J]. 植物病理学报,2001,31(4):357-364.ZHAO Ting-chang, SUN Fu-zai, WANG Bing-wan, et al. (2001). pathogen identification of Hami melon bacterial fruit blotch [J]. Acta Phyto Pathologica Sinica, 31(4):357-364. (in Chinese)
[6] 杨小丽,马俊义,胡白石,等.不同哈密瓜品种苗期对细菌性果斑病的抗性鉴定[J].新疆农业科学,2008,45(3):452-455.YANG Xiao-li, MA Jun-yi, HU Bai-shi, et al. (2008). Identification of Disease Resistance of Different Varieties of Hami Melon at Seedling Stage to Acidovoraxavenae subsp. citrulli [J]. Xinjiang Agricultural Sciences, 45(3):452-455. (in Chinese)
[7] Hopkins, D. L., Thompson, C. M., Hilgren, J., & Lovic, B. (2003). Wet seed treatment with peroxyacetic acid for the control of bacterial fruit blotch and other seedborne diseases of watermelon. Plant Disease,87(12): 1,495-1,499.
[8] Hopkins, D. L., Cucuzza, J. D., & Watterson, J. C. (1996). Wet seed treatments for the control of bacterial fruit blotch of watermelon. Plant Disease, 80(5): 529-532.
[9]胡俊,黄俊霞,刘双平,等.不同哈密瓜品种对细菌性果斑病抗病性及发展动态的研究[J].华北农学报,2006,21(6):107-110.HU Jun, HUANG Jun-xia, LIU Shuang-ping, et al. (2006). Resistance to Acidovoraxavenae subsp. citrulli and Its Disease Trends in Different Sweet melon Cultivars [J]. Journal of North China Agriculture, 21(6):107-110. (in Chinese)
[10]李俊阁,王惠林,张亮,等.不同甜瓜材料苗期对细菌性果斑病抗性鉴定[J].新疆农业科学,2015,52(10):1 843-1 848.LI Jun-ge, WANG Hui-lin, ZHANG Liang, et al. (2015). Identification of Resistance to Acidovoraxavenae subsp. Citrulli among Different Melons (Cucumis spp.) at the Seedling Stage, Xinjiang Agricultural Sciences, 52(10):1,843-1,848. (in Chinese)
[11]张亮,王惠林,万秀琴,等.不同甜瓜材料苗期细菌性果斑病抗性鉴定[J].北方园艺,2016,(9):117-120.ZHANG Liang, WANG Hui-lin, WAN Xiu-qin, et al. (2016). Identification of Resistance to Acidovoraxcitrulli of Different Melons in the Seedling Stage [J]. Northern Horticulture, (9):117-120. (in Chinese).
[12] 周贤达,睢祥琨,陈路路,等.砧木(葫芦)种子干热灭菌消毒处理研究[J]. 中国瓜菜,2013,26(2):38-39.ZHOU Xian-da, QU Xiang-kun, CHEN Lu-lu, et al. (2013).The research on dry heat sterilization of stock (cucurbit) seed [J]. China Cucurbits and Vegetables, 26(2):38-39. (in Chinese).
[13] 金岩.西瓜细菌性果斑病研究[D].长春:吉林农业大学硕士论文,2003.JIN Yan. (2003). The research on Watermelon bacterial fruit blotch [D]. Master Dissertation. Jilin Agricultural University, Chancun. (in Chinese)
[14] Hopkins, D. L., Thompson, C. M. (2002). Evaluation of Citrullus sp. Germ Plasm for Resistance to Acidovoraxavenae subsp. Citrulli. Plant Disease, 86(1): 61-63.
[15]贾宋楠,王惠林,郑健,等.籽用西瓜种质资源对细菌性果斑病的抗性鉴定[J].新疆农业科学,2013,50(5):864-869. JIA Song-nan, WANG Hui-lin, ZHEN Jian, et al. (2013). Identification of Resistance to Acidovoraxavenae subsp. Citrulli of Edible Seed Watermelon Germplasm Resources [J]. Xinjiang Agricultural Sciences, 50(5):864-869. (in Chinese)
[16]刘欣欣,王文博,潘春清,等.黑龙江省西甜瓜栽培品种及种质资源对细菌性果斑病的抗性鉴定[J].中国蔬菜,2014,(9):23-26.LIU Xin-xin, WANG Wen-bo, PAN Chun-qing, et al. (2014). Resistance Identification of Watermelon and Melon Varieties and their Germplasm Resources in Heilongjiang Province against Acidovoraxavenae [J]. China Vegetables, (9):23-26. (in Chinese)
[2] 李威,任毓忠,丁建军.新疆瓜类细菌性果斑病品种抗性鉴定[J]. 北方园艺,2007,(3):186-188.LI Wei, REN Yu-zhong, DING Jian-jun. (2007). Identification of Citrull Resistance to Acidovoraxavenae subsp. Citrulli in Xinjiang [J]. Northern Horticulture, (3):186-188. (in Chinese)
[3]王宗伟,汪文静,刘曼双.哈密瓜细菌性果斑病的发生与防治[J].新疆农垦科技,2002,(5):16-17.WANG Zong-wei, WANG Wen-jing, LIU Man-shuang. (2002). The Occurrence and Prevention of Bacterial Fruit Spot Disease of Hami Melon [J]. Xinjiang Agricultural Reclamation Technology,(5):16-17. (in Chinese)
[4]张昕,李国英,任毓忠,等.新疆哈密瓜上两种病原细菌比较鉴定及其田间消长动态的研究[J] .中国农业科学, 2002, 35(7):888-893。ZHANG Xin, LI Guo-ying, REN Yu-zhong, et al. (2002). Identification of Pathogen of Bacterial Disease of Melon and Pathogens' Alternate Predomination Investigation in Field in Xinjiang [J]. Scientia Agricultura Sinica, 35(7):888-893. (in Chinese)
[5]赵廷昌,孙福在,王兵万,等.哈密瓜细菌性果斑病病原鉴定[J]. 植物病理学报,2001,31(4):357-364.ZHAO Ting-chang, SUN Fu-zai, WANG Bing-wan, et al. (2001). pathogen identification of Hami melon bacterial fruit blotch [J]. Acta Phyto Pathologica Sinica, 31(4):357-364. (in Chinese)
[6] 杨小丽,马俊义,胡白石,等.不同哈密瓜品种苗期对细菌性果斑病的抗性鉴定[J].新疆农业科学,2008,45(3):452-455.YANG Xiao-li, MA Jun-yi, HU Bai-shi, et al. (2008). Identification of Disease Resistance of Different Varieties of Hami Melon at Seedling Stage to Acidovoraxavenae subsp. citrulli [J]. Xinjiang Agricultural Sciences, 45(3):452-455. (in Chinese)
[7] Hopkins, D. L., Thompson, C. M., Hilgren, J., & Lovic, B. (2003). Wet seed treatment with peroxyacetic acid for the control of bacterial fruit blotch and other seedborne diseases of watermelon. Plant Disease,87(12): 1,495-1,499.
[8] Hopkins, D. L., Cucuzza, J. D., & Watterson, J. C. (1996). Wet seed treatments for the control of bacterial fruit blotch of watermelon. Plant Disease, 80(5): 529-532.
[9]胡俊,黄俊霞,刘双平,等.不同哈密瓜品种对细菌性果斑病抗病性及发展动态的研究[J].华北农学报,2006,21(6):107-110.HU Jun, HUANG Jun-xia, LIU Shuang-ping, et al. (2006). Resistance to Acidovoraxavenae subsp. citrulli and Its Disease Trends in Different Sweet melon Cultivars [J]. Journal of North China Agriculture, 21(6):107-110. (in Chinese)
[10]李俊阁,王惠林,张亮,等.不同甜瓜材料苗期对细菌性果斑病抗性鉴定[J].新疆农业科学,2015,52(10):1 843-1 848.LI Jun-ge, WANG Hui-lin, ZHANG Liang, et al. (2015). Identification of Resistance to Acidovoraxavenae subsp. Citrulli among Different Melons (Cucumis spp.) at the Seedling Stage, Xinjiang Agricultural Sciences, 52(10):1,843-1,848. (in Chinese)
[11]张亮,王惠林,万秀琴,等.不同甜瓜材料苗期细菌性果斑病抗性鉴定[J].北方园艺,2016,(9):117-120.ZHANG Liang, WANG Hui-lin, WAN Xiu-qin, et al. (2016). Identification of Resistance to Acidovoraxcitrulli of Different Melons in the Seedling Stage [J]. Northern Horticulture, (9):117-120. (in Chinese).
[12] 周贤达,睢祥琨,陈路路,等.砧木(葫芦)种子干热灭菌消毒处理研究[J]. 中国瓜菜,2013,26(2):38-39.ZHOU Xian-da, QU Xiang-kun, CHEN Lu-lu, et al. (2013).The research on dry heat sterilization of stock (cucurbit) seed [J]. China Cucurbits and Vegetables, 26(2):38-39. (in Chinese).
[13] 金岩.西瓜细菌性果斑病研究[D].长春:吉林农业大学硕士论文,2003.JIN Yan. (2003). The research on Watermelon bacterial fruit blotch [D]. Master Dissertation. Jilin Agricultural University, Chancun. (in Chinese)
[14] Hopkins, D. L., Thompson, C. M. (2002). Evaluation of Citrullus sp. Germ Plasm for Resistance to Acidovoraxavenae subsp. Citrulli. Plant Disease, 86(1): 61-63.
[15]贾宋楠,王惠林,郑健,等.籽用西瓜种质资源对细菌性果斑病的抗性鉴定[J].新疆农业科学,2013,50(5):864-869. JIA Song-nan, WANG Hui-lin, ZHEN Jian, et al. (2013). Identification of Resistance to Acidovoraxavenae subsp. Citrulli of Edible Seed Watermelon Germplasm Resources [J]. Xinjiang Agricultural Sciences, 50(5):864-869. (in Chinese)
[16]刘欣欣,王文博,潘春清,等.黑龙江省西甜瓜栽培品种及种质资源对细菌性果斑病的抗性鉴定[J].中国蔬菜,2014,(9):23-26.LIU Xin-xin, WANG Wen-bo, PAN Chun-qing, et al. (2014). Resistance Identification of Watermelon and Melon Varieties and their Germplasm Resources in Heilongjiang Province against Acidovoraxavenae [J]. China Vegetables, (9):23-26. (in Chinese)