糙皮侧耳黄斑病一种新病原菌的鉴定及生物防治
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摘要
黄斑病是严重影响糙皮侧耳(Pleurotus ostreatus)产量和品质的一种细菌性病害。本研究从山西省糙皮侧耳生产区采集的黄斑病样品中分离到病原菌菌株HB-1,对其进行了形态观察、分子鉴定与生理生化指标测定并研究了醋酸杆菌菌株BA15发酵液对该病原菌的防治作用。结果表明:该病原菌与公认的糙皮侧耳病原菌托拉斯假单胞杆菌(Pseudomonas tolaasii)不同,为解淀粉芽孢杆菌(Bacillus amyloliquefaciens);醋酸杆菌BA15发酵液对HB-1具有显著的抑制作用。本研究结果为糙皮侧耳黄斑病病原菌的鉴定及生物防治提供了参考。
Yellow blotch disease(YBD)is a bacterial disease that seriously affects the yield and quality of Pleurotus ostreatus.The samples of YBD were obtained from several main production areas of P.ostreatus in Shanxi province.The pathogenic strain HB-1 was isolated from YBD samples,and further determined by morphological observation,molecular identification and physiological and biochemical profiles.The results showed that the pathogen was identified as Bacillus amyloliquefaciens,which was different from Pseudomonas tolaasii,a recognized pathogen of P.ostreatus YBD.Furthermore,the biocontrol effect of Acetobacter fermentation broth on the pathogenic strain HB-1 was investigated.The results indicated that the fermentation broth of Acetobacter BA15 fermentation broth had a significant inhibitory effect on the pathogenic strain HB-1.The results of this study provide a reference for identification and biocontrol of YBD pathogens in P.ostrea tus.
Yellow blotch disease(YBD)is a bacterial disease that seriously affects the yield and quality of Pleurotus ostreatus.The samples of YBD were obtained from several main production areas of P.ostreatus in Shanxi province.The pathogenic strain HB-1 was isolated from YBD samples,and further determined by morphological observation,molecular identification and physiological and biochemical profiles.The results showed that the pathogen was identified as Bacillus amyloliquefaciens,which was different from Pseudomonas tolaasii,a recognized pathogen of P.ostreatus YBD.Furthermore,the biocontrol effect of Acetobacter fermentation broth on the pathogenic strain HB-1 was investigated.The results indicated that the fermentation broth of Acetobacter BA15 fermentation broth had a significant inhibitory effect on the pathogenic strain HB-1.The results of this study provide a reference for identification and biocontrol of YBD pathogens in P.ostrea tus.
引文
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[16]TAJALIPOUR S,HASSANZADEH N,JOLFAEE HK,et al.Biological control of mushroom brown blotch disease using antagonistic bacteria[J].Biocontrol Science and Technology,2014,24(4):473-484.
[2]WANG LN,GAO W,WU XL,et al. Genome-Wide characterization and expression analyses of Pleurotus ostreatus MYB transcription factors during developmental stages and under heat stress based on de novo sequenced genome[J].International Journal of Molecular Sciences,2018,19:2052.
[3]HENKELS MD,KIDARSA TA,SHAFFER BT,et al.Pseudomonas protegens Pf-5 causes discoloration and pitting of mushroom caps due to the production of antifungal metabolites[J].Molecular Plant-Microbe Interactions,2014,27(7):733-746.
[4]BRUNO GL,RANA GL,SERMANI S,et al.Control of bacterial yellowing of cardoncello mushroom Plerotus eryngii using acetic or hydrochloric acid solutions[J].Crop Protection,2013,50:24-29.
[5]DOS SANTOS TL,BELAN LL,ZIED DC,et al.Essential oils in the control of dry bubble disease in white button mushroom[J].Ciencia Rural,2017,47,05:e20160780.
[6]SEBASTIEN M,MARGARITA MM,HAISSAM JM.Biological control in the microbiome era:challenges and opportunities[J].Biological Control,2015,89:98-108.
[7]王超,郭坚华,席运官,等.拮抗细菌在植物病害生物防治中应用的研究进展[J].江苏农业科学,2017,45(18):1-6.
[8]周德庆,徐德强.微生物学实验教程[M].北京:高等教育出版社,2013.
[9]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[10]张瑞颖,胡丹丹,左雪梅,等.平菇和双孢蘑菇细菌性褐斑病研究进展[J].植物保护学报,2007,34(5):549-554.
[11]GAO Z,ZHANG B,LIU H,et al.Identification of endophytic Bacillus velezensis,ZSY-1 strain and antifungal activity of its volatile compounds against Alternaria solani and Botrytis cinerea[J].Biological Control,2017,105:27-39.
[12]KIM JD,JEON BJ,HAN JW,et al. Evaluation of the endophytic nature of Bacillus amyloliquefaciens strain GYL4 and its efficacy in the control of anthracnose[J].Pest Management Science,2016,72(8):1529-1536.
[13]FAN B,BLOM J,KLENK HP,et al.Bacillus amyloliquefaciens,Bacillus velezensis,and Bacillus siamensis form an"operational group B.amyloliquefaciens"within the B.subtilis species complex[J].Frontiers in Microbiology,2017,8:22.
[14]ZHANG RY,HU DD,GU JG,et al.Evaluation of oyster mushroom strains for resistance to Pseudomonas tolaasii by inoculation in spawned substrates[J].European Journal of Plant Pathology,2013,137(1):119-126.
[15]刘川,陈强,张金霞,等.平菇细菌性黄斑病发病因素分析[J].食用菌学报,2013,20(1):101-105.
[16]TAJALIPOUR S,HASSANZADEH N,JOLFAEE HK,et al.Biological control of mushroom brown blotch disease using antagonistic bacteria[J].Biocontrol Science and Technology,2014,24(4):473-484.