核桃分离蛋白的制备及其包埋番茄红素的工艺
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摘要
研究核桃蛋白的基本组成及其作为壁材包埋番茄红素的工艺,并对包埋后番茄红素的光照稳定性进行研究。试验得出以p H为关键参数包埋番茄红素的基本工艺条件:芯壁比1︰2,单甘脂为乳化剂,添加量0.5%。在此基础上,通过正交试验设计得出最佳工艺参数:均质剪切速率9 000 r/min、包埋温度50℃、包埋时间45 min,此时包埋效率最高,为67.94%;均质剪切速率9 000 r/min、包埋温度50℃、包埋时间50 min,此时包埋产率最高,为80.60%。研究得出微胶囊化可有效提高番茄红素的光照稳定性,尤其胶囊内部番茄红素的光照稳定性。核桃蛋白作为壁材包埋番茄红素具有可行性和实际意义。
The composition of walnut protein and the processing condition of embedding lycopene by walnut protein were studied, followed by evaluation of light stability of embedded lycopene. Using pH as the key processing parameter, the basic process conditions for embedding lycopene with were as follows: core-wall ratio 1︰2, using monoglyceride as emulsifier with addition amount of 0.5%. Furthermore, orthogonal experimental design was used, and the optimum process condition was obtained. When homogenization shear rate 9 000 r/min, embedding temperature 50 ℃, and embedding time 45 min, the highest embedding efficiency was obtained(67.94%); When homogenization shear rate 9 000 r/min, embedding temperature50 ℃, and embedding time 50 min, the highest embedding yield was obtained(80.60%). Microencapsulation could greatly improve the light stability of lycopene, especially the stability of lycopene inside the capsule. Therefore, the walnut protein had the feasibility and practical significance for embedding lycopene as a wall material.
The composition of walnut protein and the processing condition of embedding lycopene by walnut protein were studied, followed by evaluation of light stability of embedded lycopene. Using pH as the key processing parameter, the basic process conditions for embedding lycopene with were as follows: core-wall ratio 1︰2, using monoglyceride as emulsifier with addition amount of 0.5%. Furthermore, orthogonal experimental design was used, and the optimum process condition was obtained. When homogenization shear rate 9 000 r/min, embedding temperature 50 ℃, and embedding time 45 min, the highest embedding efficiency was obtained(67.94%); When homogenization shear rate 9 000 r/min, embedding temperature50 ℃, and embedding time 50 min, the highest embedding yield was obtained(80.60%). Microencapsulation could greatly improve the light stability of lycopene, especially the stability of lycopene inside the capsule. Therefore, the walnut protein had the feasibility and practical significance for embedding lycopene as a wall material.
引文
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[8]NORI M P,FAVAROTRINDADE C S,SMD A,et al.Microencapsulation of propolis extract by complex coacervation[J].LWT-Food Science and Technology,2011,44(2):429-435.
[9]LI C,WANG J,SHI J,et al.Encapsulation of tomato oleoresin using soy protein isolate-gum aracia conjugates as emulsifier and coating materials[J].Food Hydrocolloids,2015,45(45):301-308.
[2]刘晓红,吴胜利,万剑真,等.全果核桃蛋白饮料稳定性研究[J].食品工业,2011,32(9):52-54.
[3]SZE-TAO K W C,SATHE S K.Walnuts(Juglans regia L):proximate composition,protein solubility,protein amino acid composition and protein in vitro digestibility[J].Journal of the Science of Food&Agriculture,2000,80(9):1393-1401.
[4]NESTERENKO A,ALRIC I,SILVESTRE F,et al Comparative study of encapsulation of vitamins with native and modified soy protein[J].Food Hydrocolloids,2014,38(6):172-179.
[5]SHU B,YU W,ZHAO Y,et al.Study on microencapsulation of lycopene by spray-drying[J].Journal of Food Engineering2006,76(4):664-669.
[6]BLANCH G P,CASTILLO M L R D,CAJA M D M,et al.Stabilization of all-trans-lycopene from tomato by encapsulation using cyclodextrins[J].Food Chemistry,2007,105(4):1335-1341.
[7]MAO X,HUA Y.Chemical composition,molecular weight distribution,secondary structure and effect of NaCl on functional properties of walnut(Juglans regia L)protein isolates and concentrates[J].Journal of Food Science and Technology,2014,51(8):1473-1482.
[8]NORI M P,FAVAROTRINDADE C S,SMD A,et al.Microencapsulation of propolis extract by complex coacervation[J].LWT-Food Science and Technology,2011,44(2):429-435.
[9]LI C,WANG J,SHI J,et al.Encapsulation of tomato oleoresin using soy protein isolate-gum aracia conjugates as emulsifier and coating materials[J].Food Hydrocolloids,2015,45(45):301-308.