采用电雾式检测器测定掺假蜂蜜中的果葡糖浆
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摘要
采用电雾式检测器建立了鉴别掺假蜂蜜的方法,实验采用乙腈和水(65∶35)流动相等度洗脱,流速1.2 mL·min~(-1),Shodex Asahipak NH2P-50 4E色谱柱(4.6×150 mm,5μm)进行分离,Corona Veo RS检测器采集频率5 Hz,蒸发温度35℃,Filter5.不同聚合度的麦芽糖在8.0—60.0μg·mL~(-1)浓度范围,线性相关系数为0.999;方法灵敏度高,1%果葡糖浆可明显检出.方法快捷高效,可用于掺假蜂蜜的快速鉴别.
A HPLC-CAD Method was established for rapidly quantification of oligosaccharides adulterated honey. We use a Shodex Asahipak NH_2P-50 4 E column( 4.6 × 150 mm,5 μm) to seperate these compounds,with the mobile phase consisting of acetonitrile and H_2O( 65∶35). The samples were detected by Corona Veo RS detector,with collect rate 5 Hz,evaporative temperature 35 ℃,Filter 5. As a result, using the chromatographic peaks of maltooligosaccharide( maltose to maltoheptaose) standards as reference,the differences of honey,fructose-glucose syrup and pure honey containing fructose-glucose syrup were detected by CAD. The linear ranges of these different malto oligosaccharides were 8.0—60.0 mg·L~(-1) and the correlation coefficients( r) were all 0.999. 1% fructose-glucose syrup in adulterated honey can be detected,this method showed high accuracy,sensitivity and short time for analysis.
A HPLC-CAD Method was established for rapidly quantification of oligosaccharides adulterated honey. We use a Shodex Asahipak NH_2P-50 4 E column( 4.6 × 150 mm,5 μm) to seperate these compounds,with the mobile phase consisting of acetonitrile and H_2O( 65∶35). The samples were detected by Corona Veo RS detector,with collect rate 5 Hz,evaporative temperature 35 ℃,Filter 5. As a result, using the chromatographic peaks of maltooligosaccharide( maltose to maltoheptaose) standards as reference,the differences of honey,fructose-glucose syrup and pure honey containing fructose-glucose syrup were detected by CAD. The linear ranges of these different malto oligosaccharides were 8.0—60.0 mg·L~(-1) and the correlation coefficients( r) were all 0.999. 1% fructose-glucose syrup in adulterated honey can be detected,this method showed high accuracy,sensitivity and short time for analysis.
引文
[1]任雪梅,胡梅,周传静,等.蜂蜜中掺入不同类型果葡糖浆的检测[J].山东农业科学,2013,45(2):117-119.REN X M,HU Mei,ZHOU C J,et al. Determination on different types of high fructose corn syrup(HFCS)adulterated in honey[J].Shandong Agriculture Sciences,2013,45(2):117-119(in Chinese).
[2]黄文诚.淀粉糖及检测技术[J].蜜蜂杂志,2010,4:18-19.HUANG W C. Starch sugar and detection technology[J].Journal of Bee,2010,4:18-19(in Chinese).
[3]国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2015.National Pharmacopoeia Committee. Pharmacopoeia of People’s Republic of China[M]. Beijing:Chemical Industry Pres,2015(in Chinese).
[4] GB/T 18932.1—2002蜂蜜中碳-4植物糖含量测定方法稳定碳同位素比率法[S].GB/T 18932.1—2002 Method for the determination of C-4 plant sugars in honey-stable carbon isotope ratio method[S].(in Chinese).
[2]黄文诚.淀粉糖及检测技术[J].蜜蜂杂志,2010,4:18-19.HUANG W C. Starch sugar and detection technology[J].Journal of Bee,2010,4:18-19(in Chinese).
[3]国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2015.National Pharmacopoeia Committee. Pharmacopoeia of People’s Republic of China[M]. Beijing:Chemical Industry Pres,2015(in Chinese).
[4] GB/T 18932.1—2002蜂蜜中碳-4植物糖含量测定方法稳定碳同位素比率法[S].GB/T 18932.1—2002 Method for the determination of C-4 plant sugars in honey-stable carbon isotope ratio method[S].(in Chinese).