藜麦尾孢叶斑病的病原鉴定
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摘要
<正>藜麦(Chenopodium quinoa)是藜科藜属1年生双子叶植物~([1]),作为一种新型粮食作物在南美洲、欧洲、大洋洲、亚洲等90多个国家种植或试种~([2])。2014年以来,在我国的山西、青海、甘肃、河北等省份开始规模化种植~([3]);山西省静乐县是我国藜麦规模化种植最早的基地~([3]),随着种植面积的扩大及连作栽培导致病害日趋严重,尤其是叶部病害。2015～2017年间,本课题组在静乐县藜麦种
A leaf spot disease on Chenopodium quinoa was found in Jingle,Shanxi. The leaf spots appeared as yellow nearly-circular spot on the surface at early stages,and gradually formed large lesions with pale brown mold at late stage. Based on analysis of LSU,rpb2 and ITS sequences,the strains used in this study were located in same cluster with Cercospora cf. chenopodii in phylogenetic tree. According to morphological characteristics,pathogenicity test,and molecular identification,the pathogen causing leaf spot on quinoa was identified as Cercospora cf. chenopodii.
A leaf spot disease on Chenopodium quinoa was found in Jingle,Shanxi. The leaf spots appeared as yellow nearly-circular spot on the surface at early stages,and gradually formed large lesions with pale brown mold at late stage. Based on analysis of LSU,rpb2 and ITS sequences,the strains used in this study were located in same cluster with Cercospora cf. chenopodii in phylogenetic tree. According to morphological characteristics,pathogenicity test,and molecular identification,the pathogen causing leaf spot on quinoa was identified as Cercospora cf. chenopodii.
引文
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[19]Gardes M,Bruns T D.ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts[J].Molecular Ecology,1993,2(2):113-118.
[2]Singh S,Singh R,Singh K V.Quinoa(Chenopodium quinoa Willd),functional superfood for today's world:a review[J].World of Science,2016,58:84-96.
[3]Ren G X,Yang X S,Yao Y.Current situation of quinoa industry in China(in Chinese)[J].Crops(作物杂志),2015,(5):1-5.
[4]Testen A L,Mckemy J M,Backman P A.First report of Passalora leaf spot of quinoa caused by Passalora dubia in the United States[J].Plant Disease,2013,97(1):139.
[5]Anonymous.Passalora leaf spot of quinoa caused by Passalora dubia in Beijing(in Chinese)[EB].Chinese Pesticide Net(中国农药网),www.agrichem.cn/news/2015/9/17/201591715252714852.shtml.
[6]Guo Y L.A new species of Passalora causing angular leaf spot of lobed kudzuvine(in Chinese)[J].Mycosystema(菌物学报),2015,34(1):10-12.
[7]Braun U.Miscellaneous notes on phytopathogenic hyphomycetes(II)[J].Mycotaxon,1995,55:223-241.
[8]Crous P W,Kang J C,Braun U.A phylogenetic redefinition of anamorphs genera in Mycosphaerella based on ITS rDNA sequences and morphology[J].Mycologia,2001,93(6):1081-1101.
[9]Cui J C,Zhou R J,Fu J F,et al.Epidemic processes and yield losses of early leaf spot and web blotch of peanut occurring together in the field(in Chinese)[J].Acta Phytopathologica sinica(植物病理学报),2016,46(2):265-272.
[10]Pei Y L,Shi T,Li C P,et al.Identification of the pathogen from cassava brown leaf spot and its biological characteristics(in Chinese)[J].Chinese Journal of Tropical Crops(热带作物学报),2013,34(5):927-934.
[11]Liu Z H,Zheng C,Huang X Y,et al.Pathogen identification and biological characteristics of eggplant leaf mold(in Chinese)[J].Plant Protection(植物保护),2014,40(3):58-64,69.
[12]Singh R,Kumar S,Saini D C,et al.Diversity of Passalora on Ficus[J].Mycological Progress,2013,12(4):637-643.
[13]Guo Y L,Liu X J.Abstract of flora fungorum sinicorum(in Chinese)[M].Beijing:Science Press,2003:1-2,65-123.
[14]Liu X J,Guo Y L.Studies on some species of the genus Cercosporidium in China(in Chinese)[J].Acta Mycologica Sinica(真菌学报),1982,1(2):88-102.
[15]Videira S I R,Groenewald J Z,Braun U,et al.All that glitters is not Ramularia[J].Studies in Mycology,2016,83:49-163.
[16]Videira S I R,Groenewald J Z,Nakashima C,et al.Mycosphaerellaceae-chaos or clarity[J].Studies in Mycology,2017,87:257-421.
[17]Sun G Y,Zhang R,Zhang Z,et al.Isolation of sooty blotch and flyspeck fungi from apple surface by picking up the thalli(in Chinese)[J].Acta Phytopathologica Sinica(植物病理学报),2003,35(5):479-480.
[18]Xie Z L,Tian F,Yu J,et al.The genetic structure analysis of Floccularia luteovirens using LUS and ITSassay(in Chinese)[J].M ycosystema(菌物学报),2015,34(1):26-37.
[19]Gardes M,Bruns T D.ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts[J].Molecular Ecology,1993,2(2):113-118.