HPLC同时测定翻黄合剂中芍药苷和盐酸小檗碱含量
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摘要
为了测定翻黄合剂中芍药苷和盐酸小檗碱的含量。采用中性氧化铝柱对供试品溶液进行处理,使目标峰不被杂质峰干扰。采用YMC-Pack Pro C18色谱柱(250×4. 6 mm),流动相为乙腈-0.15%磷酸溶液(梯度洗脱),流速为1.0 m L/min,柱温为26℃,检测波长为245 nm,建立了高效液相色谱测定方法。芍药苷和盐酸小檗碱的进样量分别在0.307～4.920μg(r=0.9996)和0. 064～1.025μg(r=0.9998)范围内与峰面积呈良好的线性关系,平均回收率分别为91.10%(RSD=1.75%,n=6)和92.06%(RSD=1.69%,n=6)。该方法简单、可靠,可以同时检测翻黄合剂中芍药苷和盐酸小檗碱的含量,为翻黄合剂的质量控制提供了依据。
A method for the content determination of paeoniflorin and berberine hydrochloride in Fanhuang mixture. Neutral alumina column was used to treat the sample solution so that the target peak was not disturbed by the impurity peak. Used YMC-Pack Pro C18 analysis column( 250 mm×4.6 mm),mobile phase of acetonitrile-0. 15% phosphoric acid aqueous solution( gradient elution),the flow rate was 1. 0 m L/min,the column temperature was 26 ℃,the detection wavelength was 245 nm. The linear ranges of paeoniflorin and berberine hydrochloride were 0.307 ～ 4.920 μg( r = 0.9996) and 0.064 ～ 1.025 μg( r = 0.9998) respectively,and their average recovery rates were 91.10%( RSD = 1.75%,n = 6) and 92.06%( RSD = 1.69%,n = 6) respectively. The method is simple and reliable,and can simultaneously detect the content of paeoniflorin and berberine hydrochloride in Fanhuang mixture,providing a basis for the quality control of Fanhuang mixture.
A method for the content determination of paeoniflorin and berberine hydrochloride in Fanhuang mixture. Neutral alumina column was used to treat the sample solution so that the target peak was not disturbed by the impurity peak. Used YMC-Pack Pro C18 analysis column( 250 mm×4.6 mm),mobile phase of acetonitrile-0. 15% phosphoric acid aqueous solution( gradient elution),the flow rate was 1. 0 m L/min,the column temperature was 26 ℃,the detection wavelength was 245 nm. The linear ranges of paeoniflorin and berberine hydrochloride were 0.307 ～ 4.920 μg( r = 0.9996) and 0.064 ～ 1.025 μg( r = 0.9998) respectively,and their average recovery rates were 91.10%( RSD = 1.75%,n = 6) and 92.06%( RSD = 1.69%,n = 6) respectively. The method is simple and reliable,and can simultaneously detect the content of paeoniflorin and berberine hydrochloride in Fanhuang mixture,providing a basis for the quality control of Fanhuang mixture.
引文
[1]张璐,龚旭昊,戴青,等.中药防治鸡大肠杆菌病的研究进展[J].中国兽药杂志,2017,51(09):61-67.Zhang L, Gong X H, Dai Q, et al. Research progress on prophylaxis and treatment of chicken colibacillosis using chinese material medica[J]. Chinese Journal of Veterinary Drug,2017,51(09):61-67.
[2]黄渭,杨康,杨敏,等.高效液相色谱法同时测定白带丸中芍药苷和盐酸小檗碱的含量[J].湖北中医药大学学报,2016,18(03):57-59.Huang W,Yang K,Yang M,el al. Content determination of paeoniflorin and berberine hydrochloride in baidai pills by HPLC[J]. Journal of Hubei University of Chinese Medicine,2016,18(03):57-59.
[3]马帅,葛洁英,周蓬. HPLC法测定白带丸中芍药苷及盐酸小檗碱的含量[J].药学研究,2015,34(07):398-400.Ma S, Ge J Y, Zhou P. Determination of paeoniflorin and berberine hydrochloride in baidai pills by HPLC[J]. Journal of Pharmaceutical Research,2015,34(07):398-400.
[4]赵旭,汪坤. HPLC同时测定栀黄止痛贴中栀子苷、大黄酸、芍药苷、盐酸小檗碱含量[J].中国中医药现代远程教育,2017,15(03):128-130.Zhao X, Wang K. Simultaneous content determination of geniposide,rhein,paeoniflorin and berberine in zhihuang zhitong paste by HPLC[J]. Chinese medicine modern distance education of china,2017,15(03):128-130.
[5]李静,钱江.高效液相色谱法同时测定复方黄连素片中芍药苷和盐酸小檗碱含量[J].中国药业,2016,25(07):61-63.Li J,Qian J. Content determination of paeoniflorin and berberine hydrochloride in compound huangliansu tablets by HPLC[J].China Pharmaceuticals,2016,25(07):61-63.
[2]黄渭,杨康,杨敏,等.高效液相色谱法同时测定白带丸中芍药苷和盐酸小檗碱的含量[J].湖北中医药大学学报,2016,18(03):57-59.Huang W,Yang K,Yang M,el al. Content determination of paeoniflorin and berberine hydrochloride in baidai pills by HPLC[J]. Journal of Hubei University of Chinese Medicine,2016,18(03):57-59.
[3]马帅,葛洁英,周蓬. HPLC法测定白带丸中芍药苷及盐酸小檗碱的含量[J].药学研究,2015,34(07):398-400.Ma S, Ge J Y, Zhou P. Determination of paeoniflorin and berberine hydrochloride in baidai pills by HPLC[J]. Journal of Pharmaceutical Research,2015,34(07):398-400.
[4]赵旭,汪坤. HPLC同时测定栀黄止痛贴中栀子苷、大黄酸、芍药苷、盐酸小檗碱含量[J].中国中医药现代远程教育,2017,15(03):128-130.Zhao X, Wang K. Simultaneous content determination of geniposide,rhein,paeoniflorin and berberine in zhihuang zhitong paste by HPLC[J]. Chinese medicine modern distance education of china,2017,15(03):128-130.
[5]李静,钱江.高效液相色谱法同时测定复方黄连素片中芍药苷和盐酸小檗碱含量[J].中国药业,2016,25(07):61-63.Li J,Qian J. Content determination of paeoniflorin and berberine hydrochloride in compound huangliansu tablets by HPLC[J].China Pharmaceuticals,2016,25(07):61-63.