河北省沿海地区鱼肉中多溴联苯醚的测定
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摘要
目的建立气相色谱-负化学离子源-质谱法检测海产品中8种代表性多溴联苯醚(polybrominated diphenyl ether, PBDEs)的分析方法。方法冻干后的样品经加速溶剂提取,硅胶氧化铝复合层析柱净化后,采用气相色谱-负化学离子源-质谱(GC-NCI-MS)联用仪分析,内标法定量。结果不同地区海鱼中PBDEs的污染水平无显著差异,Σ_(3-7)BrPBDEs的平均值为24.0ng/g湿重,BDE209的平均值为20.7ng/g湿重。海鱼中BDE209的含量最高(占样品总量的46.2%),其次是BDE47(14.9%), BDE28(1.2%)的贡献率最低。结论 PBDEs污染水平虽然不高,但与沿海居民健康密切相关,应持续关注海域环境中生物体内PBDEs的污染状况。
Objective To establish a method for the determination of 8 kinds of polybrominated diphenyl ethers(PBDEs) in marine fish from coastal area of Hebei province. Methods PBDEs were extracted from freeze-dried sample by pressurized liquid extraction(ASE), and then purified by glass column filled with silica and alumina. The samples were analyzed by gas chromatography-negative chemical ionization-mass spectrometry(GC-NCI-MS) with isotope dilution technique. Results There was no significant difference in PBDEs pollution levels among 39 marine fish samples collected from different sampling sites. The mean value of Σ_(3-7) BrPBDEs and BDE209 were 24.0 and20.7 ng/g wet weight. The content of BDE209 in marine fish was the highest(46.2%), followed by BDE47(14.9%)and BDE28(1.2%) was the lowest. Conclusion Although the level of PBDEs pollution is not high, it is closely related to the health of coastal residents, which is necessary to pay more attention to the pollution of PBDEs in marine environment.
Objective To establish a method for the determination of 8 kinds of polybrominated diphenyl ethers(PBDEs) in marine fish from coastal area of Hebei province. Methods PBDEs were extracted from freeze-dried sample by pressurized liquid extraction(ASE), and then purified by glass column filled with silica and alumina. The samples were analyzed by gas chromatography-negative chemical ionization-mass spectrometry(GC-NCI-MS) with isotope dilution technique. Results There was no significant difference in PBDEs pollution levels among 39 marine fish samples collected from different sampling sites. The mean value of Σ_(3-7) BrPBDEs and BDE209 were 24.0 and20.7 ng/g wet weight. The content of BDE209 in marine fish was the highest(46.2%), followed by BDE47(14.9%)and BDE28(1.2%) was the lowest. Conclusion Although the level of PBDEs pollution is not high, it is closely related to the health of coastal residents, which is necessary to pay more attention to the pollution of PBDEs in marine environment.
引文
[1]王丽琴,蒋京鑫,柯桢.气相色谱-质谱联用仪测定电子产品中多溴联苯和多溴联苯醚[J].分析仪器,2009,2(2):21-24.Wang LQ,Jiang JX,Ke Z.Determination of PBB and PBDE in electrotechnical Products by GC-MS[J].Anal Instrum,2009,2(2):21-24.
[2]何迎春,王正虹,李林,等.饮用水及牛奶中多溴联苯醚毒性及检测研究进展[J].现代预防医学,2012,39(13):3217-3219.He YC,Wang ZH,Li L,et al.Research progress on toxicity and detection of PBDEs in drinking water and milk[J].Mod Prev Med,2012,39(13):3217-3219.
[3]McDonald TA.A perspective on the potential health risks of PBDEs[J].Chemosphere,2002,46(5):745-755.
[4]Cao LY,Ren XM,Yang Y,et al.Hydroxylated polybrominated biphenyl ethers exert estrogenic effects via non-genomic g protein-coupled estrogen receptor mediated pathways[J].Environ Health Perspect,2018,126(5):057005.
[5]Cao LY,Zheng Z,Ren X,et al.Structure-dependent activity of polybrominated diphenyl ethers and their hydroxylated metabolites on estrogen related receptorγ:In vitro and in silico study[J].Environ Sci Technol,2018,52(15):8894-8902.
[6]Ross PS,Couillard CM,Ikonomou MG,et al.Large and growing environmental reservoirs of Deca-BDE present an emerging health risk for fish and marine mammals[J].Mar Pollut Bull,2009,58(1):7-10.
[7]Mizukawa K,Takada H,Takeuchi I,et al.Bioconcentration and biomagnifieation of polybrominated diphenyl ethers(PBDEs)through lower-trophic-level coastal marine food web[J].Mar Pollut Bull,2009,58(8):1217-1224.
[8]Darnerud PO.Toxic effects of brominated flame retardants in man and in wildlife[J].Environ Int,2003,29(6):841-853.
[9]Zhou XY,Guo J,Lin KF,et al.Leaching characteristics of heavy metals and brominated flame retardants from waste printed circuit boards[J].JHazard Mater,2013,246:96-102.
[10]Pirard C,Pauw ED,Focant JF.New strategy for comprehensive analysis of polybrominated diphenyl ethers,polychlorinated dibenzo-p-dioxins,polychlorinated dibenzofurans and polychlorinated biphenyls by gas chromatography coupled with mass spectrometry[J].J Chromatogr A,2003,998:169-181.
[11]卢大胜,林元杰,冯超,等.GC-MS/MS同时测定鱼样中39种多溴联苯醚[J].食品安全质量检测学报,2013,4(4):1024-1032.Lu DS,Lin YJ,Feng C,et al.Determination of 39 polybrominated biphenyl ethers in fish using GC-MS/MS[J].J Food Saf Qual,2013,4(4):1024-1032.
[12]Jin J,Liu WZ,Wang Y,et al.Levels and distribution of polybrominated diphenyl ethers in plant,shellfish and sediment samples from Laizhou Bay in China[J].Chemosphere,2008,71(6):1043-1050.
[13]DBS 13/005-2016食品安全地方标准动物源性食品中多溴联苯醚的测定[S].DBS 13/005-2016 Provincial food safety standard-Determination of polybrominated diphenyl ethers in animal foodstuffs[S].
[14]Liu YP,Li JG,Zhao YF,et al.Polybrominated diphenyl ethers(PBDEs)and indicator polychlorinated biphenyls(PCBs)in marine fish from four areas of China[J].Chemosphere,2011,83:168-174.
[15]江锦花,陶亮,陈莹曦.浙江近海海水养殖生物体内多溴联苯醚浓度特征及风险评价[J].台州学院学报,2015,37(6):1-7.Jiang JH,Tao L,Chen YX.Concentrations and risk of pbde congeners in organisms collected from the coastal aquaculture areas in Zhejiang[J].JTaizhou Univ,2015,37(6):1-7.
[2]何迎春,王正虹,李林,等.饮用水及牛奶中多溴联苯醚毒性及检测研究进展[J].现代预防医学,2012,39(13):3217-3219.He YC,Wang ZH,Li L,et al.Research progress on toxicity and detection of PBDEs in drinking water and milk[J].Mod Prev Med,2012,39(13):3217-3219.
[3]McDonald TA.A perspective on the potential health risks of PBDEs[J].Chemosphere,2002,46(5):745-755.
[4]Cao LY,Ren XM,Yang Y,et al.Hydroxylated polybrominated biphenyl ethers exert estrogenic effects via non-genomic g protein-coupled estrogen receptor mediated pathways[J].Environ Health Perspect,2018,126(5):057005.
[5]Cao LY,Zheng Z,Ren X,et al.Structure-dependent activity of polybrominated diphenyl ethers and their hydroxylated metabolites on estrogen related receptorγ:In vitro and in silico study[J].Environ Sci Technol,2018,52(15):8894-8902.
[6]Ross PS,Couillard CM,Ikonomou MG,et al.Large and growing environmental reservoirs of Deca-BDE present an emerging health risk for fish and marine mammals[J].Mar Pollut Bull,2009,58(1):7-10.
[7]Mizukawa K,Takada H,Takeuchi I,et al.Bioconcentration and biomagnifieation of polybrominated diphenyl ethers(PBDEs)through lower-trophic-level coastal marine food web[J].Mar Pollut Bull,2009,58(8):1217-1224.
[8]Darnerud PO.Toxic effects of brominated flame retardants in man and in wildlife[J].Environ Int,2003,29(6):841-853.
[9]Zhou XY,Guo J,Lin KF,et al.Leaching characteristics of heavy metals and brominated flame retardants from waste printed circuit boards[J].JHazard Mater,2013,246:96-102.
[10]Pirard C,Pauw ED,Focant JF.New strategy for comprehensive analysis of polybrominated diphenyl ethers,polychlorinated dibenzo-p-dioxins,polychlorinated dibenzofurans and polychlorinated biphenyls by gas chromatography coupled with mass spectrometry[J].J Chromatogr A,2003,998:169-181.
[11]卢大胜,林元杰,冯超,等.GC-MS/MS同时测定鱼样中39种多溴联苯醚[J].食品安全质量检测学报,2013,4(4):1024-1032.Lu DS,Lin YJ,Feng C,et al.Determination of 39 polybrominated biphenyl ethers in fish using GC-MS/MS[J].J Food Saf Qual,2013,4(4):1024-1032.
[12]Jin J,Liu WZ,Wang Y,et al.Levels and distribution of polybrominated diphenyl ethers in plant,shellfish and sediment samples from Laizhou Bay in China[J].Chemosphere,2008,71(6):1043-1050.
[13]DBS 13/005-2016食品安全地方标准动物源性食品中多溴联苯醚的测定[S].DBS 13/005-2016 Provincial food safety standard-Determination of polybrominated diphenyl ethers in animal foodstuffs[S].
[14]Liu YP,Li JG,Zhao YF,et al.Polybrominated diphenyl ethers(PBDEs)and indicator polychlorinated biphenyls(PCBs)in marine fish from four areas of China[J].Chemosphere,2011,83:168-174.
[15]江锦花,陶亮,陈莹曦.浙江近海海水养殖生物体内多溴联苯醚浓度特征及风险评价[J].台州学院学报,2015,37(6):1-7.Jiang JH,Tao L,Chen YX.Concentrations and risk of pbde congeners in organisms collected from the coastal aquaculture areas in Zhejiang[J].JTaizhou Univ,2015,37(6):1-7.