蕨菜多糖超声波辅助提取及其药理活性初步研究
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摘要
为优化蕨菜多糖的提取工艺,同时检测蕨菜多糖的药理学活性。实验采用超声波辅助提取法,在单因素试验的基础上,考察液料比、浸提次数、超声浸提时间、超声功率四因素对蕨菜多糖提取得率的影响。运用Design Expect 10.0软件分析,通过响应面分析法(RSM)优化提取条件,对蕨菜多糖促进小鼠脾细胞增殖能力和抑制结肠癌细胞(HTC-8)增殖能力进行分析。结果表明:蕨菜多糖的优化提取工艺为:液料比:36.2∶1;浸提次数:4次;超声浸提时间:43.1 min;超声波功率:240 W,在此条件下,蕨菜多糖提取效果最好,提取得率达8.60%。各因素对多糖提取得率的影响程度:浸提次数>液料比>超声浸提时间>超声功率。通过药理活性研究表明,本实验获得的蕨菜多糖具有脾细胞免疫增值和抑制结肠癌细胞增殖活性。
To optimize the extraction condition for the polysaccharide from Pteridium aquilinum and the pharmacological activities of polysaccharide were investigated in the study.The method of ultrasonic-assisted extraction was used,and on the basis of single factor test,the solid-liquid ratio,extraction times,extraction time and ultrasonic power were selected for the four-factor to target the influence as the yield of polysaccharide.Design expect 10.0 software was employed to analyze the test data and the response surface method(RSM) was applied to optimize the extraction conditions.Additionally the pharmacological activities of on tow radicals such as lymphocyte and colon cancer(HCT-8) cells proliferation were subsequently analyzed.The results suggested that optimum extraction consisted of a ration of the powder to reagent of 36.2∶1 and extraction times of 4 and extraction time of 43.1 min each time with the ultrasonic powder of 240 W.The extraction yield of polysaccharide from P.aquilinum reached to 8.60 %.The sequence of factors that worked on the extraction rate of polysaccharide was as follows:solid-liquid ratio > extraction times > extraction time > ultrasonic power.Moreover,the pharmacological activities experiment showed that polysaccharide extracted from P.aquilinum exhibited stronger activities to enhance hematopoiesis and inhibiting the HCT-8 cells growth.
To optimize the extraction condition for the polysaccharide from Pteridium aquilinum and the pharmacological activities of polysaccharide were investigated in the study.The method of ultrasonic-assisted extraction was used,and on the basis of single factor test,the solid-liquid ratio,extraction times,extraction time and ultrasonic power were selected for the four-factor to target the influence as the yield of polysaccharide.Design expect 10.0 software was employed to analyze the test data and the response surface method(RSM) was applied to optimize the extraction conditions.Additionally the pharmacological activities of on tow radicals such as lymphocyte and colon cancer(HCT-8) cells proliferation were subsequently analyzed.The results suggested that optimum extraction consisted of a ration of the powder to reagent of 36.2∶1 and extraction times of 4 and extraction time of 43.1 min each time with the ultrasonic powder of 240 W.The extraction yield of polysaccharide from P.aquilinum reached to 8.60 %.The sequence of factors that worked on the extraction rate of polysaccharide was as follows:solid-liquid ratio > extraction times > extraction time > ultrasonic power.Moreover,the pharmacological activities experiment showed that polysaccharide extracted from P.aquilinum exhibited stronger activities to enhance hematopoiesis and inhibiting the HCT-8 cells growth.
引文
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2 Chen ND,Chen NF,Chen CW,et al.Antioxidant activities and HPLC analysis of different parts partitioned from the ethanol extract of Pteridium aquilinum[J].Sci & Tech of Food Ind(食品工业科技),2013,34(17):49-52.
3 Chen ND,Chen NF,Wang T,et al.A Novel homoflavonoid from Pteridium Aquilinum( L.)Kuhn[J].Nat Prod Res Dev(天然产物研究与开发),2013,25:577-580.
4 Chen BL,Guo M.Determination of amino acids in Wild bracken by high performance liquid chromatography with pico-tag method[J].Chin J Chrom(色谱),1994,12:293-294.
5 Zhao GJ.Collection and processing tchnology of wild vegetables[J].Bull Agric Sci Tech(农业科技通讯),2003,10:37-37.
6 Chen NF,Zhang L.Extraction and analysis of flavonoids from Pteridium aquilinum var.latiusculum[J].Forest By-Prod and Spec in China(中国林副特产),2004,73(6):1-4.
7 Hu C,Liu YM,Dai SJ,et al.Preparative optimization and antitumor activity of selenylaed polysaccharides from the extract residue of Sarcandra glabra(Thunb)Nakai[J].J China Pharm Univ(中国药科大学学报),2018,49:109-116.
8 Ji XF,Xu HD,Liu YC,et al.Study on blood lipid and antioxidation of papaya flavonoids and polysaccharides[J].J Chin Inst Food Sci Tech(中国食品学报),2013,13(9):1-7.
9 Xie MY,Nie SP.Advances in studies on structure and function of active polysaccharides from natural products[J].J Chin Inst Food Sci Tech(中国食品学报),2010,10(2):1-11.
10 Liu MM,Zeng P,Li XT,et al.Antitumor and immunomodulation activities of polysaccharide from Phellinus baumii.[J].Int J Biol Macromol,2016,91:1199-1205.
11 Chen ND,Chen NF,Wang TT,et al.Accumulation of polysaccharides and ethanol-soluble extract in tissue-cultured and wild Dendrobium huoshanense and Dendrobium henanense[J].Nat Prod Res Dev(天然产物研究与开发),2015,27:2090-2094.
12 Hao J W,Chen N D,Chen CW,et al.Rapid quantification of polysaccharide and the main onosaccharides in Dendrobium huoshanense by near-infrared attenuated total reflectance spectroscopy[J].J Pharmaceut biomed,2018,155:331-338.
13 Jiang Z,Wang H,Wu QN.Optimization of polysaccharide extraction from Spirodela polyrrhiza by plackett-burman design combined with box-behnken response surface methodology[J].J Chin Med Mater(中药材),2015,38:1283-1286.
14 Feng Y,Chen QH,Meng XB,et al.Optimization of microwave and ultrasonic extraction of polysaccharides from Acanthopanax sessiliflorus fruits by response surface methodology[J].Food Sci(食品科学),2010,31:268-272.
15 Zhang B,Xie MY,Yin JY,et al.Optimization of ultrasonic-assisted extraction conditions of tea polysaccharides by using response surface methodology[J].Food Sci(食品科学),2008,29:234-238.
16 Ren DD,Chen G.Optimization of ultrasound-assisted extraction of polysaccharides from Okra by response surface analysis[J].Food Sci(食品科学),2011,32:143-146.
17 Zhao N,Xu XX,Zhang YJ,et al.Comparative study on the effects of lactoferricins from different sources on proliferation and cytokine secretion of spleen lymphocytes in mice[J].Mod Food Sci & Tech(现代食品科技),2015,31(10):7-13.
18 Ma SS,Wang SP,Lian ZX,et al.Study on growth inhibiting effect of streptococcus thermophilus expolysaccharide from Tibetan Kefir on human colon cancer cells[J].Food Sci(食品科学),2008,29:405-408.
19 Han R,He XQ,Liu HY,et al.Studies on the pharmacodynamics and pharmacokinetics of Paclitaxel[J].Chin J New Drug(中国新药杂志),1996,4:248-251.