维药多伞阿魏体外抗胃癌活性部位GC-MS指纹图谱的研究
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摘要
目的初步确定多伞阿魏Ferula ferulaeoides体外抗胃癌活性部位,并对其进行GC-MS指纹图谱研究。方法采用MTT法检测多伞阿魏不同提取物对胃癌细胞SGC-7901增殖的抑制作用;采用GC-MS、主成分分析对多伞阿魏体外抗胃癌活性部位指纹图谱进行研究。结果多伞阿魏氯仿提取部位对胃癌细胞SGC-7901增殖的抑制作用最强;建立了多伞阿魏体外抗胃癌活性部位GC-MS指纹图谱分析方法,确立了28个共有峰,与抑制胃癌细胞增殖作用有较强关系的11个共有峰,分别鉴定为峰1(3-甲氧基-1,2丙二醇)、2(右旋柠檬烯)、4(左旋乙酸龙脑酯)、6(乙酸松油酯)、7(2,6,10-三甲基-1,5,9-十一烷三烯)、8(α-柏木烯)、9(α-香柑油烯)、12(β-雪松烯)、21(8-表-γ-桉叶油醇)、22(γ-桉叶油醇)、23(茅苍术醇)。10批样品中共有峰成分占总成分的92%以上,其相似度达到0.9以上。结论初步确证了多伞阿魏氯仿提取部位具有抑制胃癌细胞SGC-7901增殖作用。建立的GC-MS指纹图谱方法快速、简便,具有良好的精密度、重复性、稳定性,可作为多伞阿魏活性部位的质量控制方法。
Objective To preliminarily ascertain the anti-gastric cancer active parts from the roots of Ferula ferulaeoide and to study its GC-MS fingerprint Methods The inhibitory effects of different extraction from the roots of F. ferulaeoide on the cell proliferation of SGC-7901 cells were determined with MTT colorimetric method. GC-MS fingerprint of in vitro anti-gastric cancer active parts from F. ferulaeoide was investigated and analyzed by GC-MS and principal component analysis(PCA). Results The chloroform extract showed the best inhibition on the growth of SGC-7901 cells. The method on GC-MS fingerprint of in vitro anti-gastric cancer active parts from F. ferulaeoide was established, showing 28 common characteristic peaks. The PCA demonstrated that the common peaks 1, 2, 4, 6, 7, 8, 9, 12, 21, 22, and 23 were connected closely with the in vitro anti-gastric cancer activity, and the seven compounds were 3-methoxy-1,2-propanediol, D-limonene, L-borneol acetate, terpinyl acetate, 1,5,9-undecatriene, 2,6,10-trimethyl, α-cedrene, and a-bergsmotene, β-cedrene, 8-epi-γ-eudesmol, γ-eudesmol, hinesol. Totally 28 common compounds of 10 batches of samples accounted for over 92% of the volatile contents, and the similarity of the GC-MS fingerprints from the 10 batches of samples was over 0.90. Conclusion The chloroform extract from the roots of F. ferulaeoides with potential inhibitory effect on gastric cancer SGC-7901 cells is tentatively confirmed, and needs further to verify by animal cells in vivo. The method of GC-MS fingerprinting is rapid, simple, and accurate with good reproducibility and stability, and can be used to control the quality of active parts of F. ferulaeoides.
Objective To preliminarily ascertain the anti-gastric cancer active parts from the roots of Ferula ferulaeoide and to study its GC-MS fingerprint Methods The inhibitory effects of different extraction from the roots of F. ferulaeoide on the cell proliferation of SGC-7901 cells were determined with MTT colorimetric method. GC-MS fingerprint of in vitro anti-gastric cancer active parts from F. ferulaeoide was investigated and analyzed by GC-MS and principal component analysis(PCA). Results The chloroform extract showed the best inhibition on the growth of SGC-7901 cells. The method on GC-MS fingerprint of in vitro anti-gastric cancer active parts from F. ferulaeoide was established, showing 28 common characteristic peaks. The PCA demonstrated that the common peaks 1, 2, 4, 6, 7, 8, 9, 12, 21, 22, and 23 were connected closely with the in vitro anti-gastric cancer activity, and the seven compounds were 3-methoxy-1,2-propanediol, D-limonene, L-borneol acetate, terpinyl acetate, 1,5,9-undecatriene, 2,6,10-trimethyl, α-cedrene, and a-bergsmotene, β-cedrene, 8-epi-γ-eudesmol, γ-eudesmol, hinesol. Totally 28 common compounds of 10 batches of samples accounted for over 92% of the volatile contents, and the similarity of the GC-MS fingerprints from the 10 batches of samples was over 0.90. Conclusion The chloroform extract from the roots of F. ferulaeoides with potential inhibitory effect on gastric cancer SGC-7901 cells is tentatively confirmed, and needs further to verify by animal cells in vivo. The method of GC-MS fingerprinting is rapid, simple, and accurate with good reproducibility and stability, and can be used to control the quality of active parts of F. ferulaeoides.
引文
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[19]王婷婷,石学魁,孙阳,等.红花多糖对胃癌细胞SGC-7901凋亡的形态学实验研究[J].中医药信息,2015,32(2):19-21.
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[2]沈观冕.中草药丛书:阿魏[M].乌鲁木齐:新疆人民出版社,1986.
[3]倪慧,姜传义,刘淑兰,等.阿魏属中挥发油成分的比较研究[J].中草药,1997,28(6):331-332.
[4]倪慧,姜传义,陈茂齐.新疆多伞阿魏根中挥发油成分研究[J].中成药,2001,23(1):54-57.
[5]宋东伟,赵文军,吴雪萍,等.新疆多伞阿魏化学成分研究[J].中草药,2006,37(11):1627-1629.
[6]Hu Y,Li X D,Li G Y,et al.Two novel sesquiterpenoids from the roots of Ferula ferulaeoides(Steud.)Korov.[J].Helv Chim Acta,2010,93(5):1019-1024.
[7]雷林洁,滕亮,赵欣,等多伞阿魏挥发油提取工艺及化学成分研究[J].中成药,2013,35(6):1251-1256.
[8]能元君,刘发,叶尔波,等.新疆三种阿魏对胃肠道作用的比较[J].新疆学院学报,1993,16(4):300-302.
[9]Zhai L L,Liu T,Xie H Q,et al.Inhibition effects on HBV replication by hydrophobic extracts from Ferula ferulaeoides(Steud.)Korov.[J].J Med Plants Res,2011,5(29):6581-6583.
[10]王春霞,徐芳,陈燕,等.三种常用药材的鉴别研究[J].新疆中医药,2011,29(5):50-52.
[11]丁旭,谭勇,阎莉,等.多伞阿魏的鉴别研究[J].中药材,2011,34(6):879-880.
[12]中国药典[S].一部.2010.
[13]张奕颖,邓涛,胡志芳,等.熊果酸体外抗胃癌细胞SGC-7901机制的研究[J].中草药,2006,37(4):555-558.
[14]刘丹,祝林,奉建芳.蟾酥中蟾酥毒配机类成分的分离纯化及体外抗肿瘤活性的研究[J].中成药,2010,32(6):937-940.
[15]王飒.新疆多伞阿魏体外抗胃癌活性部位指纹图谱研究[D].乌鲁木齐:新疆医科大学,2015.
[16]马斌荣.SPSS for Windows Ver.11.5在医学统计中的应用[M].第3版.北京:北京科学出版社,2004.
[17]郑磊贞,陈强.胃癌的化学治疗现状[J].胃肠病学,2005,10(3):178-181.
[18]刘金娟,杨成流,陈永强,等.鱼腥草地下茎诱导胃癌细胞SGC-7901凋亡机制的研究[J].中国药理学通报,2014,30(2):257-260.
[19]王婷婷,石学魁,孙阳,等.红花多糖对胃癌细胞SGC-7901凋亡的形态学实验研究[J].中医药信息,2015,32(2):19-21.
[20]韩红英,李国玉,王金辉.阿魏化学成分和药理作用的研究现状[J].农垦医学,2010,32(3):257-259.
[21]李晓瑾,姜林,帕丽达.新疆阿魏抗溃疡作用组分筛选研究[J].中国现代中药,2007,9(10):8-10.
[22]叶尔波,刘发,熊元君,等.新疆三种阿魏的抗炎与免疫抑制作用[J].西北药学杂志,1993,8(2):72-73.
[23]宿树兰,王团结,段金廒,等.常用树脂类药材资源分布、化学成分及药理活性研究进展[J].国际药学研究杂志,2009,36(2):109-114.