GC-MS结合保留指数法分析云南产胡萝卜籽挥发油成分
|
摘要
目的分析云南产胡萝卜籽挥发油成分。方法水蒸气蒸馏提取挥发油,气相色谱-质谱(GC-MS)结合保留指数法进行分析。结果鉴定了58种成分,占挥发油总量90.30%。其中,胡萝卜醇(21.612%)、β-蒎烯(11.067%)、α-蒎烯(8.973%)、(Z)-α-红没药烯(4.183%)、柠檬烯(3.761%)、β-石竹烯(3.336%)、β-没药烯(3.311%)、α-松油醇(3.098%)、α-柏木烯(3.089%)、石竹烯氧化物(2.679%)、(E)-α-香柠檬烯(2.561%)、罗汉柏烯(2.398%)、柏木脑(2.027%)含有量较高。通过比对保留指数,确认松香芹醇、松油醇构型分别为E型和α型。结论该方法可为云南胡萝卜籽进一步研究开发提供基础。
AIM To analyze the volatile oil in Yunnan carrot seeds.METHODS The volatile oil was extracted by steam distillation and analyzed by gas chromatography-mass spectrometer(GC-MS) combined with retention index.RESULTS Fifty-eight constituents were identified,accounting for 90.30% of the total content of volatile oil.Among them,the contents of carotol(21.612%),β-pinene(11.067%),α-pinene(8.973%),(Z)-α-bisabolene(4.183%),limonene(3.761%),β-caryophyllene(3.336%),β-bisabolene(3.311%),α-terpineol(3.098%),α-cedrene(3.089%),caryophyllene oxide(2.679%),(E)-α-bergamotene(2.561%),thujopsene(2.398%) and cedrol(2.027%) were relatively high.The configurations of pinocarveol and terpineol were identified as E-type and α-type by comparing retention indices,respectively.CONCLUSION This method can provide a basis for the further study and development of Yunnan carrot seeds.
AIM To analyze the volatile oil in Yunnan carrot seeds.METHODS The volatile oil was extracted by steam distillation and analyzed by gas chromatography-mass spectrometer(GC-MS) combined with retention index.RESULTS Fifty-eight constituents were identified,accounting for 90.30% of the total content of volatile oil.Among them,the contents of carotol(21.612%),β-pinene(11.067%),α-pinene(8.973%),(Z)-α-bisabolene(4.183%),limonene(3.761%),β-caryophyllene(3.336%),β-bisabolene(3.311%),α-terpineol(3.098%),α-cedrene(3.089%),caryophyllene oxide(2.679%),(E)-α-bergamotene(2.561%),thujopsene(2.398%) and cedrol(2.027%) were relatively high.The configurations of pinocarveol and terpineol were identified as E-type and α-type by comparing retention indices,respectively.CONCLUSION This method can provide a basis for the further study and development of Yunnan carrot seeds.
引文
[1]胡连官.胡萝卜籽油及其香料应用[J].四川化工,1991(4):39-40.
[2]党俊伟,张彩云,赵继飚.野胡萝卜净油化学成分的研究[J].安徽农学报,2009,5(10):222-223.
[3]张文叶,张峻松,贾春晓,等.超临界CO2萃取山胡萝卜籽油的脂肪酸成分分析[J].中国粮油学报,2009,24(9):87-89.
[4]秦巧慧,彭映辉,何建国,等.野胡萝卜果实精油对蚊幼虫的毒杀活性[J].中国生物防治学报,2011,27(4):418-422.
[5]孙凤,刘亚亚,王刚霞,等.新疆胡萝卜籽油脂肪酸GCMS分析[J].中国油脂,2012,37(8):82-83.
[6]刘睿婷,刘玲玲,熊梅,等.新疆胡萝卜籽挥发油的气相色谱/质谱法分析[J].中国调味品,2011,36(9):104-106.
[7]杨眉,冒德寿,李智宇,等.气相色谱/飞行时间质谱法分析格蓬油中的挥发性成分[J].食品工业科技,2013,34(9):280-284.
[8]Leffingwell.ESO 2006[DB/CD].Georgia:Bolens Aroma Chemical Information Service,2010.
[9]Glisic S B,Misic D R,Stamenic M D,et al.Supercritical carbon dioxide extraction of carrot fruit essential oil:Chemical composition and antimicrobial activity[J].Food Chem,2007,105(1):346-352.
[10]Giraud-Robert A M.The role of aromatherapy in the treatment of viral hepatitis[J].Int J Aromather,2005,15(4):183-192.
[11]沈振铎,陈晓辉,刘玉磊,等.GC法同时测定五味子挥发油中α-蒎烯、β-蒎烯、柠檬烯和乙酸龙脑酯的含量[J].药物分析杂志,2008,28(9):1470-1473.
[12]殷中琼.α-松油醇抗大肠杆菌机理研究[D]:成都:四川农业大学,2013.
[13]张岩,曲凡志,赵余庆.柏木醇的药理作用研究进展[J].沈阳药科大学学报,2014,31(7):584-588.
[14]刘晓宇,陈旭冰,陈光勇,等.β-石竹烯及其衍生物的生物活性与合成研究进展[J].林产化学与工业,2012,32(1):104-110.
[15]胡玉霞,李家平,余启荣,等.GC法测定灵丹油清咽喷雾剂中β-芹子烯的含量[J].中国药事,2011,25(1):33-35.
[16]周勇,姚三桃,翁玉春,等.香叶醇抗真菌作用的研究[J].真菌学报,1983,2(4):262-264.
[17]钟德玝.香叶醇抗胰腺癌作用及其机制的实验研究[D]:长沙:中南大学,2013.
[18]梁晟,李雅文,赵晨曦,等.GC-MS结合保留指数对中药挥发油的定性[J].分析测试学报,2008,27(1):84-87.
[2]党俊伟,张彩云,赵继飚.野胡萝卜净油化学成分的研究[J].安徽农学报,2009,5(10):222-223.
[3]张文叶,张峻松,贾春晓,等.超临界CO2萃取山胡萝卜籽油的脂肪酸成分分析[J].中国粮油学报,2009,24(9):87-89.
[4]秦巧慧,彭映辉,何建国,等.野胡萝卜果实精油对蚊幼虫的毒杀活性[J].中国生物防治学报,2011,27(4):418-422.
[5]孙凤,刘亚亚,王刚霞,等.新疆胡萝卜籽油脂肪酸GCMS分析[J].中国油脂,2012,37(8):82-83.
[6]刘睿婷,刘玲玲,熊梅,等.新疆胡萝卜籽挥发油的气相色谱/质谱法分析[J].中国调味品,2011,36(9):104-106.
[7]杨眉,冒德寿,李智宇,等.气相色谱/飞行时间质谱法分析格蓬油中的挥发性成分[J].食品工业科技,2013,34(9):280-284.
[8]Leffingwell.ESO 2006[DB/CD].Georgia:Bolens Aroma Chemical Information Service,2010.
[9]Glisic S B,Misic D R,Stamenic M D,et al.Supercritical carbon dioxide extraction of carrot fruit essential oil:Chemical composition and antimicrobial activity[J].Food Chem,2007,105(1):346-352.
[10]Giraud-Robert A M.The role of aromatherapy in the treatment of viral hepatitis[J].Int J Aromather,2005,15(4):183-192.
[11]沈振铎,陈晓辉,刘玉磊,等.GC法同时测定五味子挥发油中α-蒎烯、β-蒎烯、柠檬烯和乙酸龙脑酯的含量[J].药物分析杂志,2008,28(9):1470-1473.
[12]殷中琼.α-松油醇抗大肠杆菌机理研究[D]:成都:四川农业大学,2013.
[13]张岩,曲凡志,赵余庆.柏木醇的药理作用研究进展[J].沈阳药科大学学报,2014,31(7):584-588.
[14]刘晓宇,陈旭冰,陈光勇,等.β-石竹烯及其衍生物的生物活性与合成研究进展[J].林产化学与工业,2012,32(1):104-110.
[15]胡玉霞,李家平,余启荣,等.GC法测定灵丹油清咽喷雾剂中β-芹子烯的含量[J].中国药事,2011,25(1):33-35.
[16]周勇,姚三桃,翁玉春,等.香叶醇抗真菌作用的研究[J].真菌学报,1983,2(4):262-264.
[17]钟德玝.香叶醇抗胰腺癌作用及其机制的实验研究[D]:长沙:中南大学,2013.
[18]梁晟,李雅文,赵晨曦,等.GC-MS结合保留指数对中药挥发油的定性[J].分析测试学报,2008,27(1):84-87.