玛卡中抗氧化物质的提取工艺初探
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摘要
以云南省会泽县出产的玛卡为研究对象,采用提取物对DPPH的清除能力为考察指标,首先通过单因素试验确定适宜的乙醇体积分数、料液比、提取温度、提取时间,分别为乙醇体积分数40%、料液比1∶25 g/mL、提取温度80℃、提取时间2 h。在此基础上,设计四因素三水平正交试验,四个因素对提取物质抗氧化能力的影响大小为提取温度>提取时间>乙醇体积分数>料液比,最佳提取工艺为:浸提温度90℃、料液比1∶25 g/mL、溶剂浓度50%、水浴时间2 h。该条件下玛卡样品中抗氧化成分相当于12.05 mg/g抗坏血酸。
The maca which was produced in Huize County of Yunnan Province has been investigated in the study for the extraction technology for antioxidant components by checking the scavenging ability of DPPH. Firstly, the appropriate ethanol concentration, liquid ratio, extraction temperature and extraction time were determined by the single factor experiment which was ethanol concentration 40%, the solid-liquid ratio 1∶25 g/mL, the extraction temperature 80 ℃, and the extraction time 2 h.On this basis, four factors and three levels orthogonal test was designed, and the influence of various factors on the antioxidant capacity of the extraction material is extraction temperature>extraction time>ethanol concentration>material liquid ratio.The optimal technological conditions for the extraction were the extraction temperature 90 ℃, the ratio of material and liquid1∶25 g/mL, the concentration of solvent 50%, and the time of water bath 2 h, and the content of anti-oxidizing components in each sample was equivalent to 12.05 mg/g ascorbic acid under the condition.
The maca which was produced in Huize County of Yunnan Province has been investigated in the study for the extraction technology for antioxidant components by checking the scavenging ability of DPPH. Firstly, the appropriate ethanol concentration, liquid ratio, extraction temperature and extraction time were determined by the single factor experiment which was ethanol concentration 40%, the solid-liquid ratio 1∶25 g/mL, the extraction temperature 80 ℃, and the extraction time 2 h.On this basis, four factors and three levels orthogonal test was designed, and the influence of various factors on the antioxidant capacity of the extraction material is extraction temperature>extraction time>ethanol concentration>material liquid ratio.The optimal technological conditions for the extraction were the extraction temperature 90 ℃, the ratio of material and liquid1∶25 g/mL, the concentration of solvent 50%, and the time of water bath 2 h, and the content of anti-oxidizing components in each sample was equivalent to 12.05 mg/g ascorbic acid under the condition.
引文
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[2]李诗,阚红金,华玉祥,等.玛卡复方醇提物对改善小鼠性功能及抗疲劳功能的影响[J].药物生物技术, 2015(3):229-232.
[3] ZHENG B L, KIM C H, WOLTHOFF S, et al. Extract of Lepidium meyenii roots for pharmaceutical applications:US,US6267995[P]. 2001.
[4]张静,李慧,周雯,等.玛咖粉对小鼠的抗疲劳作用及其机制研究[J].卫生研究, 2013, 42(6):1046-1049.
[5]张洁宏,李彬,高玉秋,等.玛卡粉增强免疫力和抗疲劳功能动物实验研究[J].现代预防医学, 2014, 41(6):1096-1098.
[6]谢荣芳,瞿熙.玛卡引种及栽培技术[J].云南农业科技,2008, 4:42-43.
[7]万文婷,张志鹏,谭晓蕾,等.玛卡云南主产区发展现状调查[J].中国现代中药, 2015, 17(7):726-729.
[8]阿吉姑·阿布都热西提,买尔旦·马合木提,马合木提·乌斯满,等.维生素C清除自由基能力3种检测方法的比较[J].新疆医科大学学报, 2008, 31(5):578-579.
[9]陈奕,谢明勇,弓晓峰.黑灵芝提取物清除DPPH自由基的作用[J].天然产物研究与开发, 2006, 18(6):917-921.
[10]王新,郑萍,马小媛,等.水杉叶中抗氧化活性物质提取工艺研究[J].食品研究与开发, 2016, 37(18):36-40.
[11]金中华.抗坏血酸溶液配制方法的改进[J].环境监测管理与技术, 2001, 13(6):39-40.
[12]苑子夜,苏印泉,张强,等. DPPH法评价杜仲叶提取物的抗氧化活性[J].西北林学院学报, 2011, 26(6):119-123.