胆巴炮制对附子生物碱类成分的影响研究
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摘要
目的比较有胆附片与无胆附片的成分异同,探讨胆巴炮制与无胆炮制对附子生物碱类成分的影响。方法采用酸性染料比色法及高效液相色谱法对5种附子炮制品(生附片、黑顺片、熟附片、蒸附片、炒附片)的生物碱类成分进行分析。结果江油产生附片质量最优,去皮可以提高附子中有效成分的相对含量;有胆炮制黑顺片、熟附片均能显著降低毒性成分双酯型生物碱的含量,但同时有效成分单酯型生物碱及总生物碱流失严重;无胆蒸制或炒制附片能有效去除毒性成分并保留有效成分,"去毒存性"能力较好。结论不同炮制方法能不同程度地降低附子毒性成分双酯型生物碱含量,增加有效成分单酯型生物碱含量,达到增效减毒的目的;但有胆炮制附片存在炮制过度之嫌,无胆炮制工艺简便易行,不易引入无机杂质,具有推广和应用价值。
Objective To compare the differences between the brine processed and non-brine monkshood, and study the effects of different processing technology on the contents of alkaloids in Aconiti lateralis radix praeparata.Methods The alkaloids components of different products(Shengfupian-peeled, Heishunpian, Shufupian,Zhengfupian and Chaofupian) were analyzed by UV and HPLC method. Results The quality of the Jiangyou producing Shengfupian was better than the others,and the contents of the active components in aconite could be raised by peeling. Both Heishunpian and Shufupian pressed with brine can significantly reduce the contents of toxic component-the diester alkaloids,but the active ingredients,the monoester alkaloids and total alkaloids are greatly reduced. Non-brine Zhengfupian and Chaofupian can effectively remove the toxic components and retain the active ingredients. Conclusion Different processing technology can decrease the contents of diester alkaloids,increase the contents of monoester alkaloids,and achieve the purpose of increasing efficiency and reducing toxicity. However,the brine monkshood may be over-processed. The processing of non-brine monkshood is simple,without bringing in inorganic substances,which have promotional and application value.
Objective To compare the differences between the brine processed and non-brine monkshood, and study the effects of different processing technology on the contents of alkaloids in Aconiti lateralis radix praeparata.Methods The alkaloids components of different products(Shengfupian-peeled, Heishunpian, Shufupian,Zhengfupian and Chaofupian) were analyzed by UV and HPLC method. Results The quality of the Jiangyou producing Shengfupian was better than the others,and the contents of the active components in aconite could be raised by peeling. Both Heishunpian and Shufupian pressed with brine can significantly reduce the contents of toxic component-the diester alkaloids,but the active ingredients,the monoester alkaloids and total alkaloids are greatly reduced. Non-brine Zhengfupian and Chaofupian can effectively remove the toxic components and retain the active ingredients. Conclusion Different processing technology can decrease the contents of diester alkaloids,increase the contents of monoester alkaloids,and achieve the purpose of increasing efficiency and reducing toxicity. However,the brine monkshood may be over-processed. The processing of non-brine monkshood is simple,without bringing in inorganic substances,which have promotional and application value.
引文
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[5]陈树和.乌头类中药炮制历史沿革及发展概况[J].湖北中医杂志,2017,39(11):58-61.
[6]黄勤挽,周子渝,王瑾,等.附子炮制历史沿革研究[J].中国实验方剂学杂志,2011,17(23):269-271.
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[8]周林,李飞,任玉珍,等.附子中生物碱含量与在胆巴液中浸泡时间变化规律的研究[J].中国实验方剂学杂志,2014,20(10):44-47.
[9]匡青芬,侯大斌,孙鸿,等.附子无胆炮制品的生物碱与可溶性多糖含量检定[J].时珍国医国药,2014,25(4):850-852.
[10]吴月娇.附子饮片的质量评价研究[D].北京:北京中医药大学,2016.
[11]四川省食品药品监督管理局.四川省中药饮片炮制规范[M].成都:四川科学技术出版社,2015.
[12]叶强,郭一平,彭成.炮制方法对附子生物碱类成分的影响[J].华西药学杂志,2013,28(3):275-277.
[13]刘红梅,王江瑞,方成鑫,等.盐附子漂洗过程中钙离子含量的测定方法[J].中国科技论文,2016,11(6):615-618.
[14]杨昌林,黄志芳,张意涵,等.蒸制和烘制对附子生物碱成分含量的影响研究[J].中国中药杂志,2014,39(24):4798-4803.
[15]温瑞卿,李东辉,赵昕,等.基于化学分析的毒性中药附子炮制方法的合理性研究[J].药学学报,2013,48(2):286-290.
[16]王哲.炮附片和炒附片炮制方法传承与规范化应用研究[D].北京:北京中医药大学,2014.
[2]陈荣昌,孙桂波,张强,等.附子毒性研究进展[J].中国中药杂志,2013,38(8):1126-1129.
[3] YUE H,PI Z F,SONG F R,et al.Studies on the aconitine-type alkaloids in the roots of Aconitum Carmichaeli Debx. by HPLC/ESIMS/MSn[J].Talanta,2009,77(5):1800-1807.
[4] SUN H,NI B,ZHANG A,et al.Metabolomics study on Fuzi and its processed products using ultra-performance liquid-chromatography/electrospray-ionization synapt high-definition mass spectrometry coupled with pattern recognition analysis[J]. Analyst, 2011, 137(1):170-185.
[5]陈树和.乌头类中药炮制历史沿革及发展概况[J].湖北中医杂志,2017,39(11):58-61.
[6]黄勤挽,周子渝,王瑾,等.附子炮制历史沿革研究[J].中国实验方剂学杂志,2011,17(23):269-271.
[7]谭茂兰.黑顺片炮制过程中成分、热性、药效和毒性的变化研究[D].成都:成都中医药大学,2016.
[8]周林,李飞,任玉珍,等.附子中生物碱含量与在胆巴液中浸泡时间变化规律的研究[J].中国实验方剂学杂志,2014,20(10):44-47.
[9]匡青芬,侯大斌,孙鸿,等.附子无胆炮制品的生物碱与可溶性多糖含量检定[J].时珍国医国药,2014,25(4):850-852.
[10]吴月娇.附子饮片的质量评价研究[D].北京:北京中医药大学,2016.
[11]四川省食品药品监督管理局.四川省中药饮片炮制规范[M].成都:四川科学技术出版社,2015.
[12]叶强,郭一平,彭成.炮制方法对附子生物碱类成分的影响[J].华西药学杂志,2013,28(3):275-277.
[13]刘红梅,王江瑞,方成鑫,等.盐附子漂洗过程中钙离子含量的测定方法[J].中国科技论文,2016,11(6):615-618.
[14]杨昌林,黄志芳,张意涵,等.蒸制和烘制对附子生物碱成分含量的影响研究[J].中国中药杂志,2014,39(24):4798-4803.
[15]温瑞卿,李东辉,赵昕,等.基于化学分析的毒性中药附子炮制方法的合理性研究[J].药学学报,2013,48(2):286-290.
[16]王哲.炮附片和炒附片炮制方法传承与规范化应用研究[D].北京:北京中医药大学,2014.