柠檬精油对牙周致病菌抑制作用及其安全性初步研究
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摘要
目的:探讨柠檬精油对牙周致病菌的体外抗菌活性及对细胞增殖的影响。方法:采用微量液体稀释法测定柠檬精油对Pg、Fn、Aa、Pi的最小抑菌浓度(minimal inhibitory concentration,MIC)及最小杀菌浓度(minimum bactericidal concentration,MBC);以较低浓度的MIC为标准稀释LEO作为实验组,采用MTT法测定柠檬精油对HUVECs的毒性作用,明确抑菌浓度下LEO的安全性。结果:柠檬精油对牙周主要致病菌均有抑菌作用,Pg、Fn、Aa、Pi的MIC分别是9.0g/L、4.5g/L、4.5g/L、9.0g/L,Aa、Fn的MBC是9.0g/L,Pg、Pi的MBC未测得。1/2MIC、1/20MIC浓度的LEO能够抑制人脐静脉内皮细胞的生长,而低于1/200MIC浓度的LEO则对人脐静脉内皮细胞的生长没有影响,其中1/200MIC浓度的LEO作用明显优于0.02%的CHX。结论:体外环境中,柠檬精油对牙周致病菌Pg、Fn、Aa、Pi具有抗菌活性,低浓度应用对机体相对安全。
Objective:To investigate effects of lemon essential oil(LEO)on the growth and adherence of periodontal pathogens.Methods:Microplate dilution assays were performed to determine the antibacterial effect of LEO on Prophyromonas gingivalis(Pg),Fusobacterium nucleatum(Fn),A.actinomycetemcomitan(Aa),and P.intermedius(Pi).MTT method was employed to study the cytotoxic activity of LEO on human umbilical vein endothelial cells(HUVECs).Results:The LEO exhibited moderate antibacterial activity on Pg and Pi(Minimum inhibitory concentration:MIC =9mg/mL)and strong antibacterial activity on Fn and Aa(MIC= 4.5mg/mL).The minimal bactericidal concentration(MBC)was 9.0mg/mL(Fn and Aa).LEO showed no killing effect on Pg and Pi.The LEO's concentration(<1/200MIC)revealed no toxicity on HUVECs.Conclusion:LEO has a great potential to be used to treat periodontitis.
Objective:To investigate effects of lemon essential oil(LEO)on the growth and adherence of periodontal pathogens.Methods:Microplate dilution assays were performed to determine the antibacterial effect of LEO on Prophyromonas gingivalis(Pg),Fusobacterium nucleatum(Fn),A.actinomycetemcomitan(Aa),and P.intermedius(Pi).MTT method was employed to study the cytotoxic activity of LEO on human umbilical vein endothelial cells(HUVECs).Results:The LEO exhibited moderate antibacterial activity on Pg and Pi(Minimum inhibitory concentration:MIC =9mg/mL)and strong antibacterial activity on Fn and Aa(MIC= 4.5mg/mL).The minimal bactericidal concentration(MBC)was 9.0mg/mL(Fn and Aa).LEO showed no killing effect on Pg and Pi.The LEO's concentration(<1/200MIC)revealed no toxicity on HUVECs.Conclusion:LEO has a great potential to be used to treat periodontitis.
引文
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[2]Savage A,Eaton KA,Moles DR,et al.A systematic review of definitions of periodontitis and methods that have been used to identify this disease[J].Journal of clinical periodontology,2009,36(6)∶458-467
[3]Zambon JJ.Actinobacillus actinomycetemcomitans in human periodontal disease[J].Journal of clinical periodontology,1985,12(1)∶1-20
[4]边可胤,张向宇,靳趁心,等.柠檬精油对牙周病主要致病菌粘附作用影响的体外研究[J].口腔医学研究,2015,31(7)∶666-669
[5]王雪梅,谌徽,李雪姣,等.天然活性单萜—柠檬烯的抑菌性能研究[J].吉林农业大学学报,2010,32(1)∶24-28
[6]Garg A,Garg S,Zaneveld LJD,et al.Chemistry and pharmacology of the citrus bioflavonoid hesperidin[J].Phytotherapy Research,2001,15(8)∶655-669
[7]Tsai TH,Liu MC.Determination of extracellular hesperidin in blood and bile of anaesthetized rats by microdialysis with high-performance liquid chromatography:apharmacokinetic application[J].Journal of Chromatography B,2004,806(2)∶161-166
[8]Bae EA,Han MJ,Lee M,et al.In vitro inhibitory effect of some flavonoids on rotavirus infectivity[J].Biological&pharmaceutical bulletin,2000,23(9)∶1122-1124
[9]Bae EA,Kim DH.In vitro anti-Helicobacter pylori activity of some flavonoids and their metabolites[J].Planta medica,1999,65(5)∶442-443
[10]陈伟富,刘洪瑞,朱敏,等.人脐静脉血管内皮细胞分离与原代培养体会[J].实用医学杂志,2009,25(15)∶2415-2418