柱前衍生化高效液相色谱法分析车前草多糖特征图谱
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摘要
目的:从车前草中提取分离车前草多糖,通过柱前衍生化高效液相法(HPLC)对车前草多糖的单糖和糖醛酸组成进行研究,建立车前草多糖特征图谱。方法:采用水提醇沉法分离,纯化车前草多糖,通过对车前草多糖PMP柱前衍生化,利用高效液相色谱法对多糖组分进行分析。结果:车前草多糖是由葡萄糖、甘露糖、鼠李糖、半乳糖、葡萄糖醛酸、半乳糖醛酸、木糖、阿拉伯糖等8种单糖组成,摩尔比为2.39∶0.20∶0.65∶2.05∶0.19∶0.91∶0.21∶1.08。方法学验证结果表明本实验测定方法精密度良好,样品回收率在97.906~98.658,相对标准差在1.17%~1.72%,单糖标准曲线线性相关系数均大于0.999,说明该实验建立的方法有效可信。结论:实验建立车前草多糖的分离提取方法和利用高效液相色谱法建立多糖特征图谱的研究方法,操作简便、分离度高,稳定性和重现性良好,该方法可用于车前草多糖的单糖组分分析和为其质量控制提供参考。
Objective:The polysaccharide of psyllium was extracted from psyllium,and the polysaccharide composition of psyllium was studied by columnar derivatization and high performance liquid chromatography(HPLC). Methods:The polysaccharides were extracted by water method,and the polysaccharides were extracted by PMP column. The polysaccharide composition was analyzed by high performance liquid chromatography(HPLC). Results:The polysaccharide is composed of glucose,mannose,rhamnose,galactose,glucuronic acid,galacturonic acid,xylose and arabinose. The molar ratio was 2.39∶0.20∶0.65∶2.05∶0.19∶0.91∶0.21∶1.08. The results showed that the precision of the method was good,the recovery rate was between 97.906 and 98.658,the relative standard deviation was between 1.17% and 1.72%,and the linear correlation coefficient of the standard curve of monosaccharides was more than 0.999,which indicated that the experiment method was effective and credible. Conclusion:In this study,the method of separation and extraction of polysaccharide from psyllium and the method of monosaccharide composition analysis were established. The method was simple,stable,accurate and feasible. The method was applied to the analysis and determination of the content of polysaccharide in psyllium.
Objective:The polysaccharide of psyllium was extracted from psyllium,and the polysaccharide composition of psyllium was studied by columnar derivatization and high performance liquid chromatography(HPLC). Methods:The polysaccharides were extracted by water method,and the polysaccharides were extracted by PMP column. The polysaccharide composition was analyzed by high performance liquid chromatography(HPLC). Results:The polysaccharide is composed of glucose,mannose,rhamnose,galactose,glucuronic acid,galacturonic acid,xylose and arabinose. The molar ratio was 2.39∶0.20∶0.65∶2.05∶0.19∶0.91∶0.21∶1.08. The results showed that the precision of the method was good,the recovery rate was between 97.906 and 98.658,the relative standard deviation was between 1.17% and 1.72%,and the linear correlation coefficient of the standard curve of monosaccharides was more than 0.999,which indicated that the experiment method was effective and credible. Conclusion:In this study,the method of separation and extraction of polysaccharide from psyllium and the method of monosaccharide composition analysis were established. The method was simple,stable,accurate and feasible. The method was applied to the analysis and determination of the content of polysaccharide in psyllium.
引文
[1]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:64.
[2]杨亚军,周秋贵,曾红,等.车前草化学成分及新生物活性研究进展[J].中成药,2011,33(10):1771-1776.
[3]夏玲红,金冠钦,孙黎,等.车前草的化学成分与药理作用研究进展[J].中国药师,2013,16(2):294-296.
[4]李守汉.车前草多糖对运动大鼠抗氧化及免疫能力的影响[J].四川体育科学,2016,35(2):35-38.
[5]李官浩,杨咏洁,南昌希.车前草多糖的提取及纯化工艺研究[J].食品科技,2008,8(10):166-159.
[6]郭婕,胡春红,袁淑培,等.车前草多糖微波提取工艺研究[J].食品工业科技,2014,35(16):223-225.
[7]王向红,吉爽爽,生庆海,等.柱前衍生高效液相色谱法检测8种枣水溶性多糖的单糖组成[J].中国食品学报,2014,14(9):257-263.
[8]许杜鹃,夏泉,刘钢,等.高效液相法测定黄芪中性多糖的单糖组成[J].中国实验方剂学杂志,2008,14(9):4-6.
[9]白瑞斌,马玲玉,张培,等.苯酚-硫酸法结合校正因子法测定含半乳糖醛酸的多糖中的糖含量[J].中国药房,2017,21(28):2974-2978.
[10]黄再强,张燕飞,陈玲,等.川产葛根、粉葛总黄酮和多糖含量的对比分析[J].中国药房,2017,21(28):11-14.
[11]夏新奎,杨海霞,李纯,等.薤白多糖的分离纯化及组成分析[J].食品工业科技,2010,1(31):244-247.
[12]耿洪伟,付昊宇,石洪宇,等.红松松子壳多糖单糖组成及结构特征初步分析[J].吉林农业大学学报,2018,40(2):209.
[13]陈志辉,谭丽容,罗明,等.香菇多糖柱前衍生化高效液相色谱指纹图谱研究[J].医药导报,2015,34(5):649-655.
[14]曾荣香,张清华,管咏梅,等.柱前衍生化高效液相色谱法测定地稔多糖中单糖组成[J].中国实验方剂学,2015,21(10):73-76.
[15]张岩,赵振宇.柱前衍生化HPLC法建立苦豆子多糖特征图谱的研究[J].天津中医药,2017,9(34):63-637.
[2]杨亚军,周秋贵,曾红,等.车前草化学成分及新生物活性研究进展[J].中成药,2011,33(10):1771-1776.
[3]夏玲红,金冠钦,孙黎,等.车前草的化学成分与药理作用研究进展[J].中国药师,2013,16(2):294-296.
[4]李守汉.车前草多糖对运动大鼠抗氧化及免疫能力的影响[J].四川体育科学,2016,35(2):35-38.
[5]李官浩,杨咏洁,南昌希.车前草多糖的提取及纯化工艺研究[J].食品科技,2008,8(10):166-159.
[6]郭婕,胡春红,袁淑培,等.车前草多糖微波提取工艺研究[J].食品工业科技,2014,35(16):223-225.
[7]王向红,吉爽爽,生庆海,等.柱前衍生高效液相色谱法检测8种枣水溶性多糖的单糖组成[J].中国食品学报,2014,14(9):257-263.
[8]许杜鹃,夏泉,刘钢,等.高效液相法测定黄芪中性多糖的单糖组成[J].中国实验方剂学杂志,2008,14(9):4-6.
[9]白瑞斌,马玲玉,张培,等.苯酚-硫酸法结合校正因子法测定含半乳糖醛酸的多糖中的糖含量[J].中国药房,2017,21(28):2974-2978.
[10]黄再强,张燕飞,陈玲,等.川产葛根、粉葛总黄酮和多糖含量的对比分析[J].中国药房,2017,21(28):11-14.
[11]夏新奎,杨海霞,李纯,等.薤白多糖的分离纯化及组成分析[J].食品工业科技,2010,1(31):244-247.
[12]耿洪伟,付昊宇,石洪宇,等.红松松子壳多糖单糖组成及结构特征初步分析[J].吉林农业大学学报,2018,40(2):209.
[13]陈志辉,谭丽容,罗明,等.香菇多糖柱前衍生化高效液相色谱指纹图谱研究[J].医药导报,2015,34(5):649-655.
[14]曾荣香,张清华,管咏梅,等.柱前衍生化高效液相色谱法测定地稔多糖中单糖组成[J].中国实验方剂学,2015,21(10):73-76.
[15]张岩,赵振宇.柱前衍生化HPLC法建立苦豆子多糖特征图谱的研究[J].天津中医药,2017,9(34):63-637.