不同抗性短枝木麻黄种源苗木接种青枯病菌后酚类物质含量的变化
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摘要
为了解短枝木麻黄(Casuarina equisetifolia)抗青枯病的机理,对接种青枯病菌(Ralstonia solanacearum)后短枝木麻黄的单宁和黄酮含量变化进行了分析。结果表明,不同抗性短枝木麻黄种源小枝的总酚和单宁含量呈现不同的变化趋势,高抗、中抗种源均呈现先升高后降低的变化趋势,峰值均约为126 mg g–1,但中抗种源的峰值出现时间较晚,而易感种源则呈逐渐升高趋势。抗、感种源木麻黄接种青枯菌后,小枝中缩合单宁含量均呈现逐渐升高的趋势,但高抗种源的缩合单宁含量均显著高于易感种源,增加70.33%。抗性种源黄酮含量呈S型上升趋势,易感种源则持续缓慢升高。这表明接种青枯病菌后,抗、感短枝木麻黄种源表现出不同的防御特征,次生物质含量增幅越大,抑菌抗氧化能力越强,短枝木麻黄表现出的抗性越强。
In order to understand the resistance mechanism of casuarinas(Casuarina equisetifolia) against Ralstonia solanacearum, the changes in contents of tannin and phenols in casuarinas infected by R. solanacearum were analyzed. The results showed that the change trends of tannin and phenols contents in provenances with different resistibility were different. The contents of tannin and phenols in high-and media-resistant provenances increased at first and then decreased with the peak of 126 mg g–1, and the peak of media-resistant provenances came later. The susceptible provenances showed rising trend. After infected with R. solanacearum, the condensed tannin content increased gradually in three provenances, and that in the high-resistant provenances were 70.33%higher than that in susceptible provenances. The content of flavone in resistant provenances showed S type rising trend, while that in susceptible provenances increased slowly. Therefore, it was demonstrated that there were different defense mechanisms between resistant and susceptible provenances. The greater the content of secondary compounds increase, the stronger bacteriostasis and antioxidant activities, Casuarina equisetifolia would show higher resistance to bacterial wilt.
In order to understand the resistance mechanism of casuarinas(Casuarina equisetifolia) against Ralstonia solanacearum, the changes in contents of tannin and phenols in casuarinas infected by R. solanacearum were analyzed. The results showed that the change trends of tannin and phenols contents in provenances with different resistibility were different. The contents of tannin and phenols in high-and media-resistant provenances increased at first and then decreased with the peak of 126 mg g–1, and the peak of media-resistant provenances came later. The susceptible provenances showed rising trend. After infected with R. solanacearum, the condensed tannin content increased gradually in three provenances, and that in the high-resistant provenances were 70.33%higher than that in susceptible provenances. The content of flavone in resistant provenances showed S type rising trend, while that in susceptible provenances increased slowly. Therefore, it was demonstrated that there were different defense mechanisms between resistant and susceptible provenances. The greater the content of secondary compounds increase, the stronger bacteriostasis and antioxidant activities, Casuarina equisetifolia would show higher resistance to bacterial wilt.
引文
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[2]ZHONG C L,MANSOUR S,NAMBIAR-VEETIL M,et al.Casuarina glauca:A model tree for basic research in actinorhizal symbiosis[J].JBioscience,2013,38(4):815-823.doi:10.1007/s12038-013-9370-3.
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[4]QIAO J Q,CHEN Z Y,LIU Y Z,et al.Research progress on bacterial wilt of nightshade family[J].Acta Phytopathol Sin,2013,43(1):1-10.doi:10.3969/j.issn.0412-0914.2013.01.001.乔俊卿,陈志谊,刘邮洲,等.茄科作物青枯病研究进展[J].植物病理学报,2013,43(1):1-10.doi:10.3969/j.issn.0412-0914.2013.01.001.
[5]SALANOUBAT M,GENIN S,ARTIGUENAVE F,et al.Genome sequence of the plant pathogen Ralstonia solanacearum[J].Nature,2002,415(6871):497-502.doi:10.1038/415497a.
[6]HUANG G H,ZHONG C L,SU X H,et al.Genetic variation and structure of native and introduced Casuarina equisetifolia(L.Johnson)provenances[J].Silv Genet,2009,58(1/2/3/4/5/6):79-85.doi:10.1515/sg-2009-0010.
[7]SUN T H,LI J,WANG T,et al.Difference in protein expression of apple leaves with different resistance response to Botryosphaeria berengeriana f.sp.piricola infection[J].Acta Hort Sin,2018,45(3):409-420.doi:10.16420/j.issn.0513-353x.2017-0686.孙天骅,李佳,王涛,等.抗病与感病苹果叶片应答轮纹病菌侵染的蛋白质表达差异分析[J].园艺学报,2018,45(3):409-420.doi:10.16420/j.issn.0513-353x.2017-0686.
[8]TENG T,CAO F X,WANG M,et al.Effects of pine wood nematode(PWN)infection on the activity of PAL and the content of phenols in the host pine[J].J CS Univ For Technol,2007,27(3):124-127.doi:10.3969/j.issn.1673-923X.2007.03.013.滕涛,曹福祥,王猛,等.松材线虫侵染对松树苯丙氨酸解氨酶及酚类物质的影响[J].中南林业科技大学学报,2007,27(3):124-127.doi:10.3969/j.issn.1673-923X.2007.03.013.
[9]GUO H L,CHENG G W,CHEN J,et al.The function of phenolic metabolism in resistant mechanism of gray leaf spot of corn[J].Acta Phytopathol Sin,2003,33(4):342-346.doi:10.3321/j.issn:0412-0914.2003.04.012.郭红莲,程根武,陈捷,等.玉米灰斑病抗性反应中酚类物质代谢作用的研究[J].植物病理学报,2003,33(4):342-346.doi:10.3321/j.issn:0412-0914.2003.04.012.
[10]CHEN J,GAO H M,ZHU Y G,et al.Effect of phenols and its metabolism on activity cell wall degrading enzymes produced by Pythium aphanidermatum[J].Acta Phytopathol Sin,1996,26(2):171-176.doi:10.13926/j.cnki.apps.1996.02.019.陈捷,高洪敏,朱有釭,等.酚类物质和代谢对瓜果腐霉菌产生的细胞壁降解酶活性的影响[J].植物病理学报,1996,26(2):171-176.doi:10.13926/j.cnki.apps.1996.02.019.
[11]HU F,KONG C H,XU X H,et al.Inhibitory effect of flavones from Ageratum conyzoides on the major pathogens in citrus orchard[J].Chin J Appl Ecol,2002,13(9):1166-1168.胡飞,孔垂华,徐效华,等.胜红蓟黄酮类物质对柑桔园主要病原菌的抑制作用[J].应用生态学报,2002,13(9):1166-1168.
[12]WU Y H,ZHONG L J,ZHAO X X.Relationship among total phenol,flavonoid and PPO activity with the necrosis of tobacco infected by PVYN[J].Acta Phytopathol Sin,2007,37(4):398-402.doi:10.3321/j.issn:0412-0914.2007.04.010.吴元华,钟丽娟,赵秀香.烟草感染PVY~N后叶脉坏死与总酚、类黄酮及PPO关系研究[J].植物病理学报,2007,37(4):398-402.doi:10.3321/j.issn:0412-0914.2007.04.010.
[13]HUANG Q X,QU Y Y,YAO Z P,et al.Correlation between fusarium wilt resistance and expression levels of genes involved in flavonoid metabolism pathway in Gossypium barbadense L.[J].Acta Agron Sin,2017,43(12):1791-1801.doi:10.3724/SP.J.1006.2017.01791.黄启秀,曲延英,姚正培,等.海岛棉枯萎病抗性与类黄酮代谢途径基因表达量的相关性[J].作物学报,2017,43(12):1791-1801.doi:10.3724/SP.J.1006.2017.01791.
[14]WANG S K,WANG J,XU D P.Comparison of four different DNAextraction methods of Ralstonia solanacearum in eucalyptus and sensitivity of PCR detection[J].For Pest Dis,2007,26(5):4-7.doi:10.3969/j.issn.1671-0886.2007.05.002.王胜坤,王军,徐大平.四种桉树青枯菌DNA提取方法及PCR检测灵敏度比较[J].中国森林病虫,2007,26(5):4-7.doi:10.3969/j.issn.1671-0886.2007.05.002.
[15]LIU Y,QIN X Y,LI W Z,et al.The resistance evaluation to bacterial wilt of tobacco germplasm in Yunnan Province[J].J Plant Genet Resour,2010,11(1):10-16.刘勇,秦西云,李文正,等.抗青枯病烟草种质资源在云南省的评价[J].植物遗传资源学报,2010,11(1):10-16.
[16]WANG C Y,SUN S,WANG J.Relationship between tannin content from Casuarina equisetifolia and their resistance to Ralstonia solanacearum[J].Shandong Chem Ind,2015,44(3):77,82.doi:10.3969/j.issn.1008-021X.2015.03.024.王翠颖,孙思,王军.木麻黄抗感青枯病品系单宁含量差异[J].山东化工,2015,44(3):77,82.doi:10.3969/j.issn.1008-021X.2015.03.024.
[17]WANG C Y,SUN S,ZHOU E X,et al.Purification of total flavonoids from Casuarina equisetifolia by macroporous adsorption resin&inhibition of total flavonoids on Ralstonia solanacearum[J].Prot For Sci Technol,2012(1):11-14,32.doi:10.3969/j.issn.1005-5215.2012.01.005.王翠颖,孙思,周而勋,等.大孔吸附树脂纯化木麻黄总黄酮及对青枯菌的抑制[J].防护林科技,2012(1):11-14,32.doi:10.3969/j.issn.1005-5215.2012.01.005.
[18]MAKKAR H P S,BLUMMEL M,BOROWY N K,et al.Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods[J].J Sci Food Agric,2010,61(2):161-165.doi:10.1002/jsfa.2740610205.
[19]MAKKAR H P S.Quantification of Tannins in Tree and Shrub Foliage:A Laboratory Manual[M].Netherlands:Springer,2003:53-54.
[20]WANG X S,YANG C L,WANG S S,et al.Changes of phenols and lignin contents in alfalfa leaf damaged by Odontothrips loti[J].Chin JAppl Ecol,2014,25(6):1688-1692.王小珊,杨成霖,王森山,等.牛角花齿蓟马为害后苜蓿叶酚类物质和木质素含量的变化[J].应用生态学报,2014,25(6):1688-1692.
[21]WU Y Q,GUO Q Y.Potential resistance of tannins:Flavoniods in upland cotton against Helicpverpa armigera(Hübner)[J].Acta Ecol Sin,2001,21(2):286-289.doi:10.3321/j.issn:1000-0933.2001.02.018.武予清,郭予元.棉花单宁:黄酮类化合物对棉铃虫的抗性潜力[J].生态学报,2001,21(2):286-289.doi:10.3321/j.issn:1000-0933.2001.02.018.
[22]XU X Y,ZHANG W Q,HUANG Y H,et al.Comparison of identification methods of bacterial wilt resistance in Casuarinaceae and screening of resistant germplasm resources[J].J S China Agric Univ,2017,38(4):87-94.doi:10.7671/j.issn.1001-411X.2017.04.015.许秀玉,张卫强,黄钰辉,等.木麻黄青枯病抗性鉴定方法比较及抗病种质筛选[J].华南农业大学学报,2017,38(4):87-94.doi:10.7671/j.issn.1001-411X.2017.04.015.
[23]LIN X Q,HUANG J S,CAI S P,et al.Correlation of inclusions contents of different Casuarina spp.strains and resistibility to Anoplophora chinensis[J].J CS Univ For Technol,2014,34(5):37-41.doi:10.3969/j.issn.1673-923X.2014.05.008.林秀琴,黄金水,蔡守平,等.木麻黄内含物含量与对星天牛抗性关系的研究[J].中南林业科技大学学报,2014,34(5):37-41.doi:10.3969/j.issn.1673-923X.2014.05.008.