蝇蛆抗菌肽及其在动物生产中的应用
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摘要
蝇蛆抗菌肽是蝇蛆免疫后存在于血淋巴中的一种活性肽,有非特异性抗细菌、真菌、病毒、肿瘤细胞等生物学功能。本文对近年来已发现的蝇蛆抗菌肽的种类、生物学功能、诱导与分离纯化及其在动物生产中的应用进行综述。
Antimicrobial peptides(AMPs) of maggot larvae are a class of active peptides that exist in the hemolymph produced by the immune system after immuned.They possess non-specific inhibition activity against bacteria,virus,fungi,tumor cells,and other biological functions.In this paper,the types ov antimicrobial peptides of maggot larvae found in resent years,their biological functions,induction,isolation and purification,and its applications in animal production were summarized.
Antimicrobial peptides(AMPs) of maggot larvae are a class of active peptides that exist in the hemolymph produced by the immune system after immuned.They possess non-specific inhibition activity against bacteria,virus,fungi,tumor cells,and other biological functions.In this paper,the types ov antimicrobial peptides of maggot larvae found in resent years,their biological functions,induction,isolation and purification,and its applications in animal production were summarized.
引文
[1]安春菊,李德森,杜荣骞.家蝇幼虫中与抗菌功能相关的蛋白和多肽的分析[J].卫生研究,2004,1:86~88.
[2]陈冰,曹俊明,陈平洁,等.家蝇抗菌肽对凡纳滨对虾生长性能及免疫相关指标的影响[J].中国水产科学,2010,17(2):258~266.
[3]陈号,刘海燕,刘芳,等.腹泻鸡粪培养蝇蛆抗菌肽对大肠杆菌感染雏鸡疗效的观察[J].石河子大学学报(自然科学版),2013,31(3):328~332.
[4]陈红,刘邦辉,方彰胜,等.蝇蛆抗菌肽对日本鳗鲡生长性能的影响[J].黑龙江水产,2015,2:31~33.
[5]陈平洁.家蝇抗菌肽对黄颡鱼生长和白细胞吞噬活性的影响[A].中国水产学会.2007年中国水产学会学术年会暨水产微生态调控技术论坛论文摘要汇编[C].广西:中国水产学会,2007:1.
[6]程璟侠,樊宏英,赵瑞君,等.家蝇幼虫抗菌肽对K562细胞膜的质量浓度阈值作用[J].中国媒介生物学及控制杂志,2012,23(1):42~47.
[7]党向利,田金环,易辉玉,等.桔小实蝇抗菌肽Bactrocerin-2的分离纯化与鉴定[J].热带作物学报,2012,33(3):556~561.
[8]宫霞,胡树凯,乐国伟.家蝇幼虫抗菌肽MDL-2的电泳制备及其抗菌作用机理[J].昆虫学报,2007,12:1212~1218.
[9]郭礼荣.天然动物抗生素---蝇蛆抗菌肽在防治仔猪腹泻中的应用[A].中国畜牧兽医学会.中国畜牧兽医学会2013年学术年会论文集[C].北京:中国畜牧兽医学会,2013:1.
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[11]焦莉萍.家蝇幼虫抗菌肽defensin基因的克隆、表达、纯化及抑菌、抑肿瘤活性研究:[硕士学位论文][D].山西:山西医科大学,2013.
[12]李林,孙育平,黄燕华,等.家蝇抗菌肽的提取及其对凡纳滨对虾抗病力的影响[J].广东农业科学,2015,42(9):120~125.
[13]柳峰松,王丽娜,唐婷,等.家蝇抗菌肽Diptericin基因的克隆与分析[J].昆虫学报,2009,52(10):1078~1082.
[14]刘玉,赞哈尔,何晓辉,等.白痢鸡粪培养蝇蛆抗菌肽对白痢沙门菌感染雏鸡的疗效观察[J].西北农林科技大学学报(自然科学版),2015,43(7):24~28.
[15]彭凯,吴薇,龙蕾,等.断奶仔猪的腹泻研究进展[J].饲料研究,2015,13:10~15,21.
[16]丘继新,刘邦辉,方彰胜,等.蝇蛆抗菌肽对鳙鱼生长性能的影响[J].河北渔业,2014,8:10~11.
[17]屈军梅,陈平洁,李文平,等.莫家蝇抗菌肽提取及对鸡大肠杆菌病药效试验[J].中国畜牧兽医,2006,3:56~58.
[18]曲琪环,韩岚岚,周玉岩,等.家蝇抗菌肽的作用及在农业上的应用前景[J].饲料博览,2006,1:25~27.
[19]冉琳,何钢,刘嵬,等.大头金蝇幼虫抗菌肽提取纯化及抑菌活性研究[J].食品科技,2017,42(10):248~253.
[20]申红,王俊刚,秦文彬,等.饲喂蝇蛆抗菌肽对白痢鸡血液生化指标的影响[J].石河子大学学报(自然科学版),2011,29(5):566~569.
[21]唐亚丽.家蝇抗菌肽的分离及对细菌壁膜和DNA的作用:[博士学位论文][D].无锡:江南大学,2009.
[22]王芙蓉,艾辉,雷朝亮,等.家蝇幼虫组织匀浆液的抗病毒活性[J].昆虫知识,2006,1:82~85.
[23]张晓宇,彭赛君,吕月琴.抗菌肽及其在水产养殖业的应用进展[J].饲料研究2017,22,1~5+9.
[24]邹锋.家蝇抗菌肽生产与制剂研究:[硕士学位论文][D].湖南:湖南农业大学,2007.
[25]周钢.蝇蛆抗菌肽的诱导及对鸡白痢治疗效果研究:[硕士学位论文][D].新疆:石河子大学,2013.
[26]周义文,尹一兵,涂植光,等.家蝇抗菌肽抗菌活性及抗菌机制的初步研究[J].中国抗生素杂志,2004,5:272~274.
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[34]Chemysh S,Kim S I,Bekker G,et al.Antiviral and antitumor peptides from insects[J].Proc Natl Acad Sci U S A.2002,99(20):12628~12632.
[35]Chu F J,Jin X B,Xu Y Y,et al.Inflammatory Regulation Effect and Action Mechanism of Anti-Inflammatory Effective Parts of Housefly(Musca domestica)Larvae on Atherosclerosis.[J].Evid Based Complement Alternat Med,2013,2013:340267.
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[38]Fehlbaum P,Bulet P,Michaut L,et al.Insect immunity.Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence homology to plant antifungal peptides[J].J Biol Chem,1994,269(52):33159~33163.
[39]Feng Q,Huang Y,Chen M,et al.Functional synergy ofα-helical antimicrobial?peptides and traditional antibiotics against Gramnegative and Gram-positive bacteria in vitro and in vivo.[J]Eur JClin Microbiol Infect Dis,2015,34(1):197~204.
[40]Chu F J,Jin X B,Zhu J Y.Housefly maggots(Musca domestica)protein-enriched fraction/extracts(PE)inhibit lipopolysaccharide-induced atherosclerosis pro-inflammatory responses[J].J.Atheroscler.Thromb.,2011,22:282~290.
[41]Guo G,Tao R,Li Y,et al.Identification and characterization of a novel antimicrobial protein from the housefly Musca domestica[J].Biochemical&Biophysical Research Communications,2017,490(3):746~752.
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[2]陈冰,曹俊明,陈平洁,等.家蝇抗菌肽对凡纳滨对虾生长性能及免疫相关指标的影响[J].中国水产科学,2010,17(2):258~266.
[3]陈号,刘海燕,刘芳,等.腹泻鸡粪培养蝇蛆抗菌肽对大肠杆菌感染雏鸡疗效的观察[J].石河子大学学报(自然科学版),2013,31(3):328~332.
[4]陈红,刘邦辉,方彰胜,等.蝇蛆抗菌肽对日本鳗鲡生长性能的影响[J].黑龙江水产,2015,2:31~33.
[5]陈平洁.家蝇抗菌肽对黄颡鱼生长和白细胞吞噬活性的影响[A].中国水产学会.2007年中国水产学会学术年会暨水产微生态调控技术论坛论文摘要汇编[C].广西:中国水产学会,2007:1.
[6]程璟侠,樊宏英,赵瑞君,等.家蝇幼虫抗菌肽对K562细胞膜的质量浓度阈值作用[J].中国媒介生物学及控制杂志,2012,23(1):42~47.
[7]党向利,田金环,易辉玉,等.桔小实蝇抗菌肽Bactrocerin-2的分离纯化与鉴定[J].热带作物学报,2012,33(3):556~561.
[8]宫霞,胡树凯,乐国伟.家蝇幼虫抗菌肽MDL-2的电泳制备及其抗菌作用机理[J].昆虫学报,2007,12:1212~1218.
[9]郭礼荣.天然动物抗生素---蝇蛆抗菌肽在防治仔猪腹泻中的应用[A].中国畜牧兽医学会.中国畜牧兽医学会2013年学术年会论文集[C].北京:中国畜牧兽医学会,2013:1.
[10]黄伟光.蝇蛆蛋白的提取及其抗菌活性研究:[硕士学位论文][D].福州:福建农林大学,2011.
[11]焦莉萍.家蝇幼虫抗菌肽defensin基因的克隆、表达、纯化及抑菌、抑肿瘤活性研究:[硕士学位论文][D].山西:山西医科大学,2013.
[12]李林,孙育平,黄燕华,等.家蝇抗菌肽的提取及其对凡纳滨对虾抗病力的影响[J].广东农业科学,2015,42(9):120~125.
[13]柳峰松,王丽娜,唐婷,等.家蝇抗菌肽Diptericin基因的克隆与分析[J].昆虫学报,2009,52(10):1078~1082.
[14]刘玉,赞哈尔,何晓辉,等.白痢鸡粪培养蝇蛆抗菌肽对白痢沙门菌感染雏鸡的疗效观察[J].西北农林科技大学学报(自然科学版),2015,43(7):24~28.
[15]彭凯,吴薇,龙蕾,等.断奶仔猪的腹泻研究进展[J].饲料研究,2015,13:10~15,21.
[16]丘继新,刘邦辉,方彰胜,等.蝇蛆抗菌肽对鳙鱼生长性能的影响[J].河北渔业,2014,8:10~11.
[17]屈军梅,陈平洁,李文平,等.莫家蝇抗菌肽提取及对鸡大肠杆菌病药效试验[J].中国畜牧兽医,2006,3:56~58.
[18]曲琪环,韩岚岚,周玉岩,等.家蝇抗菌肽的作用及在农业上的应用前景[J].饲料博览,2006,1:25~27.
[19]冉琳,何钢,刘嵬,等.大头金蝇幼虫抗菌肽提取纯化及抑菌活性研究[J].食品科技,2017,42(10):248~253.
[20]申红,王俊刚,秦文彬,等.饲喂蝇蛆抗菌肽对白痢鸡血液生化指标的影响[J].石河子大学学报(自然科学版),2011,29(5):566~569.
[21]唐亚丽.家蝇抗菌肽的分离及对细菌壁膜和DNA的作用:[博士学位论文][D].无锡:江南大学,2009.
[22]王芙蓉,艾辉,雷朝亮,等.家蝇幼虫组织匀浆液的抗病毒活性[J].昆虫知识,2006,1:82~85.
[23]张晓宇,彭赛君,吕月琴.抗菌肽及其在水产养殖业的应用进展[J].饲料研究2017,22,1~5+9.
[24]邹锋.家蝇抗菌肽生产与制剂研究:[硕士学位论文][D].湖南:湖南农业大学,2007.
[25]周钢.蝇蛆抗菌肽的诱导及对鸡白痢治疗效果研究:[硕士学位论文][D].新疆:石河子大学,2013.
[26]周义文,尹一兵,涂植光,等.家蝇抗菌肽抗菌活性及抗菌机制的初步研究[J].中国抗生素杂志,2004,5:272~274.
[27]Ai H,Wang F,Zhang N,et al.Antiviral,Immunomodulatory,and Free Radical Scavenging Activities of a Protein-Enriched Fraction from the Larvae of the Housefly,Musca domestica[J].Journal of Insect Science,2013,13(112):1~16.
[28]Akiyam N,Hijikata M,Kobayashi A.Anti-tumor effect of N-beta-alanyl-5-S-glutathionyldihydroxyphenylalanine(5-S-GAD)a novel anti-bacterial substance from an insect[J].Anticancer Res,2000,20(1A):357~362.
[29]Baba K,Okada M,Kawano T,et al.Purification of Sarcotoxin I-II,a New Antibacterial Protein of[J].Journal of Biochemistry,2008,102(1):69~74.
[30]Boman H G,Nilsson I,Rasmuson B.Inducible antibacterial defence system in drosophila[J].Nature,1972,237(5352):232~235.
[31]Boulanger N,Munks R J,Hamilton J V,et al.Epithelial innate immunity.A novel antimicrobial peptide with antiparasitic activity in the blood-sucking insect Stomoxys calcitrans.[J].Journal of Biological Chemistry,2002,277(51):49921~49926
[32]Bulet P,Dimarcq J L,Hetru C,et al.A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution.[J].Journal of Biological Chemistry,1993,268(20):14893~14897.
[33]Ce rovsky V,Z D rek J,Fucík V,et al.Lucifensin,the longsought antimicrobial factor of medicinal maggots of the blowfly Lucilia sericata.[J].Cellular&Molecular Life Sciences,2010,67(3):455~466.
[34]Chemysh S,Kim S I,Bekker G,et al.Antiviral and antitumor peptides from insects[J].Proc Natl Acad Sci U S A.2002,99(20):12628~12632.
[35]Chu F J,Jin X B,Xu Y Y,et al.Inflammatory Regulation Effect and Action Mechanism of Anti-Inflammatory Effective Parts of Housefly(Musca domestica)Larvae on Atherosclerosis.[J].Evid Based Complement Alternat Med,2013,2013:340267.
[36]Dimarcq J L,Al E.Characterization and transcriptional profiles of a Drosophila gene encoding an insect defensin.A study in insect immunity.[J].Eur J Biochem,1994,221(1):201~209.
[37]Ekengren S,Hultmark D.Drosophila cecropin as an antifungal agent.[J].Insect Biochemistry&Molecular Biology,1999,29(11):965~972.
[38]Fehlbaum P,Bulet P,Michaut L,et al.Insect immunity.Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence homology to plant antifungal peptides[J].J Biol Chem,1994,269(52):33159~33163.
[39]Feng Q,Huang Y,Chen M,et al.Functional synergy ofα-helical antimicrobial?peptides and traditional antibiotics against Gramnegative and Gram-positive bacteria in vitro and in vivo.[J]Eur JClin Microbiol Infect Dis,2015,34(1):197~204.
[40]Chu F J,Jin X B,Zhu J Y.Housefly maggots(Musca domestica)protein-enriched fraction/extracts(PE)inhibit lipopolysaccharide-induced atherosclerosis pro-inflammatory responses[J].J.Atheroscler.Thromb.,2011,22:282~290.
[41]Guo G,Tao R,Li Y,et al.Identification and characterization of a novel antimicrobial protein from the housefly Musca domestica[J].Biochemical&Biophysical Research Communications,2017,490(3):746~752.
[42]Ai H,Wang F,Lei C.Antioxidant activities of protein-enriched fraction from the larvae of housefly,Musca domestica[J].Nat.Prod.Res.,2008,22:507~515.
[43]Hwangbo J,Hong E C,Jang A,et al.Utilization of house flymaggots,a feed supplement in the production of broiler chickens[J].Journal of enviromental Biology,2009,30(4):609~914.
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