番茄粗提物对香蕉枯萎病菌FocTR4的抑制效果
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摘要
番茄提取物对多种植物病原菌具有抑制作用,目前尚无其对香蕉枯萎病病原菌尖孢镰刀菌古巴专化型(Foc)抑制作用的报道。比较了‘倍盈’番茄根、茎、叶粗提物对Foc热带4号小种(FocTR4)生长的影响。结果表明,番茄根、茎、叶粗提物均可抑制FocTR4的生长,抑制效果依次为:叶>茎>根。此外,高浓度番茄叶和茎粗提物(100.0 g·L~(-1))对香蕉枯萎病的防治效果优于中浓度(66.7 g·L~(-1))和低浓度(50.0 g·L~(-1)),而中浓度根粗提物的抑制效果略优于高浓度。
Tomato extract has inhibitory effects on a variety of plant pathogens. To date, however, there is no report on its inhibition of banana wilt pathogen Fusarium oxysporum f.sp.Cubense(Foc). In this study, the effects of tomato extracts of tomato roots, stems and leaves on Foc tropical race 4(FocTR4) were compared. The results showed that all the tomato crude extracts could inhibit the growth of FocTR4, and the inhibitory effects followed the order of leaf > stem > root. Moreover, the inhibitory effect of high concentration(100.0 g·L~(-1)) leaf and stem crude extracts on banana wilt was better than medium(66.7 g·L~(-1)) or low(50.0 g·L~(-1)) concentration. However, the inhibitory effect of medium concentration of root crude extracts was slightly better than that of high concentration.
Tomato extract has inhibitory effects on a variety of plant pathogens. To date, however, there is no report on its inhibition of banana wilt pathogen Fusarium oxysporum f.sp.Cubense(Foc). In this study, the effects of tomato extracts of tomato roots, stems and leaves on Foc tropical race 4(FocTR4) were compared. The results showed that all the tomato crude extracts could inhibit the growth of FocTR4, and the inhibitory effects followed the order of leaf > stem > root. Moreover, the inhibitory effect of high concentration(100.0 g·L~(-1)) leaf and stem crude extracts on banana wilt was better than medium(66.7 g·L~(-1)) or low(50.0 g·L~(-1)) concentration. However, the inhibitory effect of medium concentration of root crude extracts was slightly better than that of high concentration.
引文
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[24] ZUO C W,ZHANG W N,CHEN Z J,et al.RNA sequencing reveals that endoplasmic reticulum stress and disruption of membrane integrity underlie dimethyl trisulfide toxicity against Fusarium oxysporum f.sp.cubense tropical race 4[J].Frontiers in Microbiology,2017,8:1 365.
[25] JIANG Y N,WANG W X,XIE Q J,et al.Plants transfer lipids to sustain colonization by mutualistic mycorrhizal and parasitic fungi[J].Science,2017,356(6343):1 172-1 175.
[26] 李伟明,陈晶晶,段雅婕,等.香蕉枯萎病菌热带4号小种对番茄等3种模式植物的致病性分析[J].园艺学报,2018,45(4):743-752.
[27] FRIEDMAN M.Tomato glycoalkaloids:role in the plant and in the diet[J].Journal of Agricultural and Food Chemistry,2002,50(21):5 751-5 780.
[28] 李银平,林忠东,李小斌,等.北疆连作棉田轮作倒茬模式的研究[J].干旱地区农业研究,2010,28(1):243-246,254.
[2] SANDROCK R W,VANETTEN H D.Fungal sensitivity to and enzymatic degradation of the phytoanticipin α-tomatine[J].Phytopathology,1998,88(2):137-143.
[3] 李建光,张露.番茄碱的分离纯化和抑菌活性的研究[J].新疆医科大学学报,2014,37(9):1 151-1 154.
[4] 付佳佳,刘强.番茄茎、叶中挥发性成分抑菌作用研究[J].河南农业,2015(14):46-47.
[5] 常慧芳,彭丹丹,刘强.番茄茎叶粗提液活性成分抑菌作用的研究[J].广州化工,2014,42(17):65-66.
[6] 王彦章,朱璇,卞生珍.番茄成熟过程中番茄碱含量的变化及其抑菌作用[J].新疆农业大学学报,2000,23(2):35-37.
[7] 杨从军,孟昭礼,郭景,等.番茄茎叶提取物对8种植物病原菌的生物活性初步研究[J].植物保护,2005,31(1):28-31.
[8] 杨柳,陈宇飞.番茄茎叶提取物抑菌试验[J].食品与机械,2014,30(4):126-128,160.
[9] PLOETZ R C.Fusarium wilt of banana[J].Phytopathology,2015,105(12):1 512-1 521.
[10] 刘海瑞,许文耀,林成辉.利用枯萎病菌粗毒素筛选香蕉抗性突变体[J].亚热带农业研究,2007,3(3):231-234.
[11] 刘炳钻,魏远竹.香蕉枯萎病在中国的风险分析及适生区预测[J].亚热带农业研究,2009,5(3):167-172.
[12] 孙雪丽,郝向阳,王天池,等.香蕉枯萎病防控和抗病育种研究进展[J].果树学报,2018,35(7):870-879.
[13] 黄永红,李春雨,魏岳荣,等.韭菜对香蕉枯萎病菌的拮抗及其对盆栽香蕉枯萎病发生的抑制作用[J].浙江农业学报,2011,23(6):1 162-1 166.
[14] 李吉庆,庄雪峰,林金国,等.楠木心材提取物的抑菌活性[J].森林与环境学报,2017,37(3):272-276.
[15] 冯旭,邱骥鹏,李扬,等.番茄叶化学成分研究[J].湖北农业科学,2017,56(21):4 127-4 130.
[16] 陶永霞,刘洪海,王忠民,等.番茄生物碱粗提物抑菌作用的研究[J].天然产物研究与开发,2006,18(3):438-440.
[17] ARNESON P,DURBIN R D.Studies on the mode of action of tomatine as a fungitoxic agent[J].Plant Physiology,1968,43(5):683-686.
[18] ITKIN M,ROGACHEV I,ALKAN N,et al.Glycoalkaloid metabolism1 is required for steroidal alkaloid glycosylation and prevention of phytotoxicity in tomato[J].The Plant Cell,2011,23(12):4 507-4 525.
[19] IRVING J R G W,FONTAINE T D,DOOLITTLE S P.Lycopersicin,a fungistatic agent from the tomato plant[J].Science,1945,102(2636):9-11.
[20] FRIEDMAN M,LEVIN C E.α-tomatine content in tomato and tomato products determined by HPLC with pulsed amperometric detection[J].Journal of Agricultural and Food Chemistry,1995,43(6):1 507-1 511.
[21] KOZUKUE N,HAN J S,LEE K R,et al.Dehydrotomatine and α-tomatine content in tomato fruits and vegetative plant tissues[J].Journal of Agricultural and Food Chemistry,2004,52(7):2 079-2 083.
[22] FRIEDMAN M,LEVIN C E.Dehydrotomatine content in tomatoes[J].Journal of Agricultural and Food Chemistry,1998,46(11):4 571-4 576.
[23] FRIEDMAN M,LEVIN C E,MCDONALD G M.α-tomatine determination in tomatoes by HPLC using pulsed amperometric detection[J].Journal of Agricultural and Food Chemistry,1994,42(9):1 959-1 964.
[24] ZUO C W,ZHANG W N,CHEN Z J,et al.RNA sequencing reveals that endoplasmic reticulum stress and disruption of membrane integrity underlie dimethyl trisulfide toxicity against Fusarium oxysporum f.sp.cubense tropical race 4[J].Frontiers in Microbiology,2017,8:1 365.
[25] JIANG Y N,WANG W X,XIE Q J,et al.Plants transfer lipids to sustain colonization by mutualistic mycorrhizal and parasitic fungi[J].Science,2017,356(6343):1 172-1 175.
[26] 李伟明,陈晶晶,段雅婕,等.香蕉枯萎病菌热带4号小种对番茄等3种模式植物的致病性分析[J].园艺学报,2018,45(4):743-752.
[27] FRIEDMAN M.Tomato glycoalkaloids:role in the plant and in the diet[J].Journal of Agricultural and Food Chemistry,2002,50(21):5 751-5 780.
[28] 李银平,林忠东,李小斌,等.北疆连作棉田轮作倒茬模式的研究[J].干旱地区农业研究,2010,28(1):243-246,254.