细胞块免疫组化染色技术在浆膜腔积液细胞诊断中的应用
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摘要
目的探讨以预糊化淀粉为支架的新细胞块免疫组化染色技术在浆膜腔积液细胞诊断中的应用价值。方法收集该院2015-01~2016-09接收的浆膜腔积液标本226例,分别做传统细胞涂片和以预糊化淀粉为支架制作细胞块,根据诊断需要选做免疫组化染色。结果传统细胞涂片阳性检出率为25/226(11.06%),细胞块结合免疫组化阳性检出率为48/226(21.24%),明显优于传统细胞涂片(P<0.05)。结论以预糊化淀粉为支架的新细胞块技术操作简单,切片质量优良,结合免疫组化染色明显提高了浆膜腔积液恶性肿瘤细胞的检出率,并有助于确定转移性肿瘤的组织来源。
Objective To investigate the application value of a new cell block technique based on pre-gelatinized starch combined with immunohistochemistry in cytodiagnosis of dropsy of serous cavity. Methods 226 specimens of dropsy of serous cavity were collected. Traditional cell smears and cell blocks based on pre-gelatinized starch were made,and immunohistochemical staining was performed according to the diagnosis. Results The positive rate of the traditional cell smears( 11. 06%) was significantly lower than that of the cell block combined with immunohistochemistry( 21. 24%)( P < 0. 05). Conclusion The new cell block technique based on pre-gelatinized starch is simple in operation,good in slicing quality and good in immunohistochemical staining. Cell block combined with immunohistochemistry can improve the detection rate of malignant tumor cells and determine the source of metastatic tumors in dropsy of serous cavity.
Objective To investigate the application value of a new cell block technique based on pre-gelatinized starch combined with immunohistochemistry in cytodiagnosis of dropsy of serous cavity. Methods 226 specimens of dropsy of serous cavity were collected. Traditional cell smears and cell blocks based on pre-gelatinized starch were made,and immunohistochemical staining was performed according to the diagnosis. Results The positive rate of the traditional cell smears( 11. 06%) was significantly lower than that of the cell block combined with immunohistochemistry( 21. 24%)( P < 0. 05). Conclusion The new cell block technique based on pre-gelatinized starch is simple in operation,good in slicing quality and good in immunohistochemical staining. Cell block combined with immunohistochemistry can improve the detection rate of malignant tumor cells and determine the source of metastatic tumors in dropsy of serous cavity.
引文
1 He QL,Zhu YZ,Zheng GJ,et al.A new convenient technique for making cell blocks[J].Cell Tissue Res,2012,350(2):395-400.
2 张尽晖,黄利小,王琪.CEA、NSE、CY_(21-1)、GLU在结核性、恶性胸腔积液鉴别诊断中的意义[J].中国临床新医学,2009,2(4):359-362.
3 罗凌,刘航,梁大华.神经元特异性烯醇化酶对癌性胸水诊断价值的Meta分析[J].中国临床新医学,2013,6(2):118-121.
4 赵银环,林梅,张艳,等.胸腹水细胞块的改良技术[J].临床和实验医学杂志,2015,31(4):469-470.
5 刘辉,胡艳萍,吴林林,等.体液沉淀物包埋石蜡制片的病理应用[J].诊断病理学杂志,2016,23(9):716-717.
6 唐丽艳.细胞块技术在浆膜腔积液细胞学诊断中的应用[J].临床和实验医学杂志,2016,15(3):279-281.
7 黄雅萍,蔡路兵,翟梅娟,等.胸腹水细胞沉渣包埋技术及应用[J].临床与实验病理学杂志,2004,20(1):112-123.
8 柳玮华,余小蒙,周小鸽.凝血酶细胞块简捷制备法[J].临床与实验病理学杂志,2007,23(6):721-722.
9 彭先高,罗孟疌.浆膜腔积液脱落细胞学检查方法的改进[J].临床与实验病理学杂志,2010,26(4):504-505.
10 程新宇,吴璠,孙虓,等.冷冻组织包埋剂在浆膜腔积液细胞块制作中应用[J].临床与实验病理学杂志,2013,29(10):1145-1147.
11 Collins GR,Thomas J,Joshi N,et al.The diagnostic value of cell block as an adjunct to liquid-based cytology of bronchial washing specimens in the diagnosis and subclassification of pulmonary neoplasms[J].Cancer Cytopathol,2012,120(2):134-141.
12 罗丽花,张婉仪,刘惠娟,等.细胞块联合免疫组化在胸腔积液诊断中的应用[J].诊断病理学杂志,2015,31(8):904-907.
13 黄智勇,苏海燕,林燕玲,等.细胞块联合免疫组化染色在恶性浆膜腔积液诊断中的应用[J].齐齐哈尔医学院学报,2016,37(23):2873-2875.
14 Zhu YZ,Cui FY,Yang Y,et al.Optimized pregelatinized starch technique for cell block preparation in Cell cultures[J].Exp Mol Pathol,2013,95(2):144-150.
2 张尽晖,黄利小,王琪.CEA、NSE、CY_(21-1)、GLU在结核性、恶性胸腔积液鉴别诊断中的意义[J].中国临床新医学,2009,2(4):359-362.
3 罗凌,刘航,梁大华.神经元特异性烯醇化酶对癌性胸水诊断价值的Meta分析[J].中国临床新医学,2013,6(2):118-121.
4 赵银环,林梅,张艳,等.胸腹水细胞块的改良技术[J].临床和实验医学杂志,2015,31(4):469-470.
5 刘辉,胡艳萍,吴林林,等.体液沉淀物包埋石蜡制片的病理应用[J].诊断病理学杂志,2016,23(9):716-717.
6 唐丽艳.细胞块技术在浆膜腔积液细胞学诊断中的应用[J].临床和实验医学杂志,2016,15(3):279-281.
7 黄雅萍,蔡路兵,翟梅娟,等.胸腹水细胞沉渣包埋技术及应用[J].临床与实验病理学杂志,2004,20(1):112-123.
8 柳玮华,余小蒙,周小鸽.凝血酶细胞块简捷制备法[J].临床与实验病理学杂志,2007,23(6):721-722.
9 彭先高,罗孟疌.浆膜腔积液脱落细胞学检查方法的改进[J].临床与实验病理学杂志,2010,26(4):504-505.
10 程新宇,吴璠,孙虓,等.冷冻组织包埋剂在浆膜腔积液细胞块制作中应用[J].临床与实验病理学杂志,2013,29(10):1145-1147.
11 Collins GR,Thomas J,Joshi N,et al.The diagnostic value of cell block as an adjunct to liquid-based cytology of bronchial washing specimens in the diagnosis and subclassification of pulmonary neoplasms[J].Cancer Cytopathol,2012,120(2):134-141.
12 罗丽花,张婉仪,刘惠娟,等.细胞块联合免疫组化在胸腔积液诊断中的应用[J].诊断病理学杂志,2015,31(8):904-907.
13 黄智勇,苏海燕,林燕玲,等.细胞块联合免疫组化染色在恶性浆膜腔积液诊断中的应用[J].齐齐哈尔医学院学报,2016,37(23):2873-2875.
14 Zhu YZ,Cui FY,Yang Y,et al.Optimized pregelatinized starch technique for cell block preparation in Cell cultures[J].Exp Mol Pathol,2013,95(2):144-150.