根施5-氨基乙酰丙酸对亚适宜温光下黄瓜幼苗生长的影响
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摘要
为缓解亚适宜温光环境对黄瓜幼苗生长产生的不利影响,以中农26号为试验材料,研究亚适宜温光下根施不同浓度5-氨基乙酰丙酸(ALA)对黄瓜幼苗生长和生理特性的影响。结果表明,亚适宜温光下,根施5 mg·L~(-1) ALA可以显著提高黄瓜幼苗的全株干重、壮苗指数和根系活力,与对照相比,黄瓜幼苗全株干重和壮苗指数分别提高了32.5%和47.1%,根系活力提高了95.0%。根施5 mg·L~(-1) ALA能够显著增加幼苗叶片叶绿素和ALA含量,与对照相比,总叶绿素和ALA的含量分别提高了49.5%和55.7%。根施5 mg·L~(-1) ALA黄瓜幼苗的净光合速率(Pn)和气孔导度(Gs)分别较对照提高了22.9%和42.2%。根施5 mg·L~(-1)ALA后,抗氧化酶活性显著提高,叶片和根系的丙二醛(MDA)含量分别比对照降低了35.3%和18.1%。同时,根施适宜浓度的ALA对矿质元素的吸收和运输产生有一定影响,但对叶绿素合成过程中相关基因的表达无明显调控作用。本研究为缓解黄瓜幼苗亚适宜温光伤害提供了新方法,也为ALA在设施农业上的应用提供了技术支持。
In order to alleviate the adverse effects of suboptimal temperature and light intensity on the growth of cucumber seedlings, cucumber(cv. Zhongnong 26) was used as test material to carry out the effects of different concentrations of exogenous ALA on growth and physiological characteristics of cucumber seedlings under suboptimal temperature and light intensity. The results showed that root application 5 mg·L~(-1) exogenous ALA could significantly increase the dry mass, vigorous seedling index and root activity under suboptimal temperature and light compared with the control. The dry mass of whole plant and vigorous seedling index of cucumber seedlings were significantly increased by 32.5% and 47.1%, respectively, and the root activity were significantly increased by 95.0% and the contents of total chlorophyll and ALA in leaves of seedlings were increased by 49.5% and 55.7%, respectively. Moreover, the net photosynthetic rate and stomatal conductance of cucumber seedlings increased by 22.9% and 42.2%, respectively, compared with the control. In addition, after treated with 5 mg·L~(-1) ALA, the activities of antioxidant enzymes were significantly increased, and the content of malondialdehyde(MDA) in leaf and roots decreased by 35.3% and 18.1%, respectively. Root application of ALA can regulate absorption and transport regulation of mineral elements, but there was no obvious regulation on the expression of related genes in chlorophyll synthesis. The results of this study provide a new method for alleviating the injury of cucumber seedlings under suboptimal temperature and light intensity, and offer technical support for ALA application in facility agriculture.
In order to alleviate the adverse effects of suboptimal temperature and light intensity on the growth of cucumber seedlings, cucumber(cv. Zhongnong 26) was used as test material to carry out the effects of different concentrations of exogenous ALA on growth and physiological characteristics of cucumber seedlings under suboptimal temperature and light intensity. The results showed that root application 5 mg·L~(-1) exogenous ALA could significantly increase the dry mass, vigorous seedling index and root activity under suboptimal temperature and light compared with the control. The dry mass of whole plant and vigorous seedling index of cucumber seedlings were significantly increased by 32.5% and 47.1%, respectively, and the root activity were significantly increased by 95.0% and the contents of total chlorophyll and ALA in leaves of seedlings were increased by 49.5% and 55.7%, respectively. Moreover, the net photosynthetic rate and stomatal conductance of cucumber seedlings increased by 22.9% and 42.2%, respectively, compared with the control. In addition, after treated with 5 mg·L~(-1) ALA, the activities of antioxidant enzymes were significantly increased, and the content of malondialdehyde(MDA) in leaf and roots decreased by 35.3% and 18.1%, respectively. Root application of ALA can regulate absorption and transport regulation of mineral elements, but there was no obvious regulation on the expression of related genes in chlorophyll synthesis. The results of this study provide a new method for alleviating the injury of cucumber seedlings under suboptimal temperature and light intensity, and offer technical support for ALA application in facility agriculture.
引文
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[8] 吴雯雯, 安玉艳, 汪良驹. 5–氨基乙酰丙酸缓解‘红颜’草莓盐胁迫伤害的时间效应研究[J]. 园艺学报, 2017, 44(6): 1038-1048
[9] 唐晓清, 王康才, 肖云华, 汪良驹. 5-氨基乙酰丙酸(ALA)对遮光环境下菘蓝的生长、叶片气孔气体参数和生物碱含量的影响[J]. 生态学杂志, 2013, 32(5): 1155-1160
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[11] Manafi E, Sanavy S, Aghaalikhani M, Dolatabadian A. Exogenous 5-aminolevulenic acid promotes antioxidative defence system, photosynthesis and growth in soybean against cold stress[J]. Notulae Scientia Biologicae, 2015, 7(4): 486-494
[12] Zhang J, Li D M, Gao Y, Yu B, Xia C-X, Bai J-G. Pretreatment with 5-aminolevulinic acid mitigates heat stress of cucumber leaves[J]. Biologia Plantarum, 2012, 56(4): 780-784
[13] Liu D, Hu L Y, Ali B, Yang A G, Wan G L, Xu L, Zhou W J. Influence of 5-aminolevulinic acid on photosynthetically related parameters and gene expression in Brassica napus L. under drought stress[J]. Soil Science & Plant Nutrition, 2016, 62(3): 254-262
[14] Kanto U, Jutamanee K, Osotsapar Y, Chaiarree W, Jattupornpong S. Promotive effect of priming with 5-aminolevulinic acid on seed germination capacity, seedling growth and antioxidant enzyme activity in rice subjected to accelerated ageing treatment[J]. Plant Production Science, 2015, 18(4): 443-454
[15] Memon S A, Wang L J, Hou X L. Effect of 5-aminolevulinic acid (ALA) on antioxidative enzymes, chlorophyll content, and photosynthesis of Pakchoi (Brassica campestris ssp. chinensis) under salt stress[J]. Middle East Journal of Scientific Research, 2013, 18(7): 899-906
[16] 常青山, 张利霞, 米银法, 沙晨霞, 王倩丽, 黄玥, 赵运发, 关俊超, 余玉磊, 韩晓鹏. 外源ALA对盐胁迫下夏枯草幼苗抗氧化能力与光合特性的影响[J]. 核农学报, 2017, 31(10): 2055-2062
[17] 鄢岩, 贺会强, 陈振东, 陶衡, 邹志荣, 杨晓林, 郭福霞. 5-氨基乙酰丙酸和叶面肥对荒漠区设施番茄和辣椒生长发育、产量和品质的影响[J]. 西北农业学报, 2016, 25(10): 1515-1521
[18] 燕飞. 外源5-氨基乙酰丙酸(ALA)对盐胁迫下黄瓜幼苗生理调控效应研究[D]. 杨凌: 西北农林科技大学, 2014
[19] 杨晴, 郭守华. 植物生理生化[M]. 北京:中国农业科学技术出版社, 2010
[20] 王小平, 项苏留. 微波消解-ICP-OES,AAS和AFS测定大蒜不同部位20种元素含量[J]. 光谱学与光谱分析, 2006, 26(10): 1907-1911
[21] 王平荣, 张帆涛, 高家旭, 孙小秋, 邓晓建. 高等植物叶绿素生物合成的研究进展[J]. 西北植物学报, 2009, 29(3): 629-636
[22] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△Ctmethod[J]. Methods, 2001,25(4): 402-408
[23] 徐晓洁. 外源ALA对NaCl胁迫下番茄植株生理生化特性的影响[D]. 杨凌: 西北农林科技大学, 2008
[24] 柳翠霞, 罗庆熙, 李月建. 外源5-氨基乙酰丙酸(ALA)对弱光下黄瓜生长指标及抗氧化酶活性的影响[J]. 中国蔬菜, 2011(16): 72-78
[25] Korkmaz A, Korkmaz Y, Demirk1ran A R. Enhancing chilling stress tolerance of pepper seedlings by exogenous application of 5-aminolevulinic acid[J]. Environmental & Experimental Botany, 2010, 67(3): 495-501
[26] 郭晓青. 亚适宜温光条件下ALA对番茄生理特性的影响[D]. 泰安: 山东农业大学,2011
[27] 杨蕊, 邹志荣, 祁向玲. ALA和CaCl2处理对黄瓜植株初花期、结果期盐胁迫的缓解效应[J]. 干旱地区农业研究, 2008, 26(4): 136-140
[28] 刘卫琴, 康琅, 汪良驹. ALA对草莓光合作用的影响及其与抗氧化酶的关系[J]. 西北植物学报, 2006, 26(1): 57-62
[29] Tanaka Y, Tanaka A, Tsuji H. Effects of 5-aminolevulinic acid on the accumulation of chlorophyll b and apoproteins of the light-harvesting chlorophyll a/b-protein complex of photosystem Ⅱ[J]. Plant & Cell Physiology, 1993, 34(3): 465-472
[30] 刘旦. 5-氨基乙酰丙酸调控干旱胁迫下油菜幼苗生长的生理机制研究[D]. 杭州: 浙江大学, 2013
[31] 康琅, 程云, 汪良驹. 5-氨基乙酰丙酸对秋冬季大棚西瓜叶片光合作用及抗氧化酶活性的影响[J]. 西北植物学报, 2006, 26(11): 2297-2301
[32] Shen P, Yang J, Wei W, Li Y, Li D, Zeng H, Wang J. Effects of fluorescent light-guided transurethral resection on non-muscle-invasive bladder cancer: a systematic review and meta-analysis[J].British Journal of Urology International, 2012, 110(6b): 209-215
[33] Liu D, Wu L, Naeem M S, Liu H, Deng X, Xu L, Zhang F, Zhou W. 5-Aminolevulinic acid enhances photosynthetic gas exchange, chlorophyll fluorescence and antioxidant system in oilseed rape under drought stress[J]. Acta Physiologiae Plantarum, 2013, 35(9): 2747-2759
[34] 陈罡, 管安琴, 万云龙, 冯伟民, 樊平声, 卢昱宇. 外源5-氨基乙酰丙酸(ALA)对盐胁迫下小型西瓜幼苗抗氧化酶活性的影响[J]. 江苏农业科学, 2016, 44(6): 252-255
[35] 张丽颖, 冯新新, 高晶晶, 安玉艳, 田凡, 李洁, 张治平, 汪良驹. 根际浇灌ALA溶液对苹果叶片生理特性与果实品质的影响[J]. 江苏农业学报, 2015, 31(1): 158-165
[2] 张小翠, 刘玉梅, 白龙强, 贺超兴, 于贤昌, 李衍素. 亚适宜温光环境下不同硝铵比营养液对黄瓜幼苗生长、氮吸收和代谢的影响[J].应用生态学报, 2016, 27(8): 2527-2534
[3] 邓惠惠.亚适宜温光下GA3对黄瓜生长和生理特性的影响[D]. 泰安: 山东农业大学, 2016
[4] 刘桂红, 闫妍, 张铁耀, 杜金哲, 于贤昌, 李衍素. 氮对亚适宜温光下黄瓜生理特性和产量的影响[J]. 核农学报, 2014, 28(6): 1108-1115
[5] 徐刚, 刘涛, 高文瑞, 孙艳军, 李德翠. 5-氨基乙酰丙酸对蔬菜生理作用的研究进展[J]. 金陵科技学院学报, 2010, 26(4): 52-57
[6] 李利兰, 罗庆熙. ALA对蔬菜生理生化影响的研究进展[J]. 长江蔬菜, 2012(8): 11-13
[7] 赵艳艳. 外源5-氨基乙酰丙酸缓解番茄幼苗盐胁迫的效果及机理研究[D]. 杨凌: 西北农林科技大学, 2014
[8] 吴雯雯, 安玉艳, 汪良驹. 5–氨基乙酰丙酸缓解‘红颜’草莓盐胁迫伤害的时间效应研究[J]. 园艺学报, 2017, 44(6): 1038-1048
[9] 唐晓清, 王康才, 肖云华, 汪良驹. 5-氨基乙酰丙酸(ALA)对遮光环境下菘蓝的生长、叶片气孔气体参数和生物碱含量的影响[J]. 生态学杂志, 2013, 32(5): 1155-1160
[10] 高文瑞, 徐刚, 李德翠, 王显生, 孙艳军, 韩冰, 史珑燕. 外源5-氨基乙酰丙酸(ALA)对辣椒幼苗抗冷性的影响[J]. 西南农业学报, 2015, 28(5):2205-2208
[11] Manafi E, Sanavy S, Aghaalikhani M, Dolatabadian A. Exogenous 5-aminolevulenic acid promotes antioxidative defence system, photosynthesis and growth in soybean against cold stress[J]. Notulae Scientia Biologicae, 2015, 7(4): 486-494
[12] Zhang J, Li D M, Gao Y, Yu B, Xia C-X, Bai J-G. Pretreatment with 5-aminolevulinic acid mitigates heat stress of cucumber leaves[J]. Biologia Plantarum, 2012, 56(4): 780-784
[13] Liu D, Hu L Y, Ali B, Yang A G, Wan G L, Xu L, Zhou W J. Influence of 5-aminolevulinic acid on photosynthetically related parameters and gene expression in Brassica napus L. under drought stress[J]. Soil Science & Plant Nutrition, 2016, 62(3): 254-262
[14] Kanto U, Jutamanee K, Osotsapar Y, Chaiarree W, Jattupornpong S. Promotive effect of priming with 5-aminolevulinic acid on seed germination capacity, seedling growth and antioxidant enzyme activity in rice subjected to accelerated ageing treatment[J]. Plant Production Science, 2015, 18(4): 443-454
[15] Memon S A, Wang L J, Hou X L. Effect of 5-aminolevulinic acid (ALA) on antioxidative enzymes, chlorophyll content, and photosynthesis of Pakchoi (Brassica campestris ssp. chinensis) under salt stress[J]. Middle East Journal of Scientific Research, 2013, 18(7): 899-906
[16] 常青山, 张利霞, 米银法, 沙晨霞, 王倩丽, 黄玥, 赵运发, 关俊超, 余玉磊, 韩晓鹏. 外源ALA对盐胁迫下夏枯草幼苗抗氧化能力与光合特性的影响[J]. 核农学报, 2017, 31(10): 2055-2062
[17] 鄢岩, 贺会强, 陈振东, 陶衡, 邹志荣, 杨晓林, 郭福霞. 5-氨基乙酰丙酸和叶面肥对荒漠区设施番茄和辣椒生长发育、产量和品质的影响[J]. 西北农业学报, 2016, 25(10): 1515-1521
[18] 燕飞. 外源5-氨基乙酰丙酸(ALA)对盐胁迫下黄瓜幼苗生理调控效应研究[D]. 杨凌: 西北农林科技大学, 2014
[19] 杨晴, 郭守华. 植物生理生化[M]. 北京:中国农业科学技术出版社, 2010
[20] 王小平, 项苏留. 微波消解-ICP-OES,AAS和AFS测定大蒜不同部位20种元素含量[J]. 光谱学与光谱分析, 2006, 26(10): 1907-1911
[21] 王平荣, 张帆涛, 高家旭, 孙小秋, 邓晓建. 高等植物叶绿素生物合成的研究进展[J]. 西北植物学报, 2009, 29(3): 629-636
[22] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△Ctmethod[J]. Methods, 2001,25(4): 402-408
[23] 徐晓洁. 外源ALA对NaCl胁迫下番茄植株生理生化特性的影响[D]. 杨凌: 西北农林科技大学, 2008
[24] 柳翠霞, 罗庆熙, 李月建. 外源5-氨基乙酰丙酸(ALA)对弱光下黄瓜生长指标及抗氧化酶活性的影响[J]. 中国蔬菜, 2011(16): 72-78
[25] Korkmaz A, Korkmaz Y, Demirk1ran A R. Enhancing chilling stress tolerance of pepper seedlings by exogenous application of 5-aminolevulinic acid[J]. Environmental & Experimental Botany, 2010, 67(3): 495-501
[26] 郭晓青. 亚适宜温光条件下ALA对番茄生理特性的影响[D]. 泰安: 山东农业大学,2011
[27] 杨蕊, 邹志荣, 祁向玲. ALA和CaCl2处理对黄瓜植株初花期、结果期盐胁迫的缓解效应[J]. 干旱地区农业研究, 2008, 26(4): 136-140
[28] 刘卫琴, 康琅, 汪良驹. ALA对草莓光合作用的影响及其与抗氧化酶的关系[J]. 西北植物学报, 2006, 26(1): 57-62
[29] Tanaka Y, Tanaka A, Tsuji H. Effects of 5-aminolevulinic acid on the accumulation of chlorophyll b and apoproteins of the light-harvesting chlorophyll a/b-protein complex of photosystem Ⅱ[J]. Plant & Cell Physiology, 1993, 34(3): 465-472
[30] 刘旦. 5-氨基乙酰丙酸调控干旱胁迫下油菜幼苗生长的生理机制研究[D]. 杭州: 浙江大学, 2013
[31] 康琅, 程云, 汪良驹. 5-氨基乙酰丙酸对秋冬季大棚西瓜叶片光合作用及抗氧化酶活性的影响[J]. 西北植物学报, 2006, 26(11): 2297-2301
[32] Shen P, Yang J, Wei W, Li Y, Li D, Zeng H, Wang J. Effects of fluorescent light-guided transurethral resection on non-muscle-invasive bladder cancer: a systematic review and meta-analysis[J].British Journal of Urology International, 2012, 110(6b): 209-215
[33] Liu D, Wu L, Naeem M S, Liu H, Deng X, Xu L, Zhang F, Zhou W. 5-Aminolevulinic acid enhances photosynthetic gas exchange, chlorophyll fluorescence and antioxidant system in oilseed rape under drought stress[J]. Acta Physiologiae Plantarum, 2013, 35(9): 2747-2759
[34] 陈罡, 管安琴, 万云龙, 冯伟民, 樊平声, 卢昱宇. 外源5-氨基乙酰丙酸(ALA)对盐胁迫下小型西瓜幼苗抗氧化酶活性的影响[J]. 江苏农业科学, 2016, 44(6): 252-255
[35] 张丽颖, 冯新新, 高晶晶, 安玉艳, 田凡, 李洁, 张治平, 汪良驹. 根际浇灌ALA溶液对苹果叶片生理特性与果实品质的影响[J]. 江苏农业学报, 2015, 31(1): 158-165