茎瘤芥对硒的吸收、转化及其富集特征研究
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摘要
为了探讨硒在茎瘤芥中的吸收、富集机制,本试验采用不同浓度(0、1、2.5 mg/L)的Na_2SeO_3进行茎瘤芥叶面喷施田间试验,研究茎瘤芥对硒的吸收、转化和利用规律,以及硒对茎瘤芥生长的影响。结果表明,在适宜硒浓度(1 mg/L)下,供试作物茎瘤芥产量提高,未达到显著水平;茎瘤芥中硝酸盐含量下降到最低点,还原糖、游离氨基酸和Vc则为相反趋势,在Se 1 mg/L上升到最高,分别与对照组相比增加了46.73%、40.41%和13.36%;茎瘤芥总硒含量和有机硒含量随硒浓度升高而增加,且呈显著正相关,较对照分别增加了74.0%～271.5%和46.1%～193.9%,而茎瘤芥有机硒转化率也会随硒浓度的增加而显著增加,较对照组分别上升了23.57%和19.33%。
To explore the absorption and enrichment mechanism of exogenous selenium in Brassica tumida Tsenet Lee, we carried out field experiments with foliar spraying different concentrations of Na_2Se0_3(0, 1, 2.5 mg/L), and studied the rules of selenium absorption, transformation and utilization of Brassica tumida Tsenet Lee, and the effect of selenium on the growth of Brassica tumida Tsenet Lee. The results showed that: at the suitable concentration of Se(1 mg/L), the yield of Brassica tumida Tsenet Lee increased but the difference was not significance; the content of nitrate in Brassica tumida Tsenet Lee reduced to the lowest point by the foliar spraying, while the content of reducing sugar, free amino acid and Vc showed an opposite trend and reached the highest point with the same concentration(1 mg/L),compared with the control,increased by 46.73%,40.41%, 13.36%, respectively; with the increasing of foliar spraying Se, the concentration of total Se and organic Se increased by 74.0%-271.5% and 46.1%-193.9%, respectively, compared with the control, and showed a significant positive correlation; with the increasing concentration of Se in Brassica tumida Tsenet Lee, the transformation rate of organic Se significantly increased by 23.57% and 19.33% compared with the control respectively.
To explore the absorption and enrichment mechanism of exogenous selenium in Brassica tumida Tsenet Lee, we carried out field experiments with foliar spraying different concentrations of Na_2Se0_3(0, 1, 2.5 mg/L), and studied the rules of selenium absorption, transformation and utilization of Brassica tumida Tsenet Lee, and the effect of selenium on the growth of Brassica tumida Tsenet Lee. The results showed that: at the suitable concentration of Se(1 mg/L), the yield of Brassica tumida Tsenet Lee increased but the difference was not significance; the content of nitrate in Brassica tumida Tsenet Lee reduced to the lowest point by the foliar spraying, while the content of reducing sugar, free amino acid and Vc showed an opposite trend and reached the highest point with the same concentration(1 mg/L),compared with the control,increased by 46.73%,40.41%, 13.36%, respectively; with the increasing of foliar spraying Se, the concentration of total Se and organic Se increased by 74.0%-271.5% and 46.1%-193.9%, respectively, compared with the control, and showed a significant positive correlation; with the increasing concentration of Se in Brassica tumida Tsenet Lee, the transformation rate of organic Se significantly increased by 23.57% and 19.33% compared with the control respectively.
引文
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[21]陈雪,沈方科,梁欢婷,等.外源施硒措施对水稻产量品质及植株硒分布的影响[J].南方农业学报,2017,48(1):46-50.
[22]梁春红,康云艳,柴喜荣,等.硒营养对菜心(菜薹)植株生长、产量和品质的影响[J].广东农业科学,2014,41(07):33-37.
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[29]陶爱芬,陈娴娴,祁建民,等.外源硒及脯氨酸对菜用黄麻生长和生理特性的影响[J].中国麻业科学,2015,37(05):239-245.
[30]张琳,孙炎鑫,廖上强.叶面喷施硒对生菜长势和品质的影响[J/OL].现代农业科技,2017(12):61-62.
[31]Al-Kanani T,MacKenzie A F,Barthakur N N.Soil-water and ammonia volatilization relationships with surface-applied nitrogenfertilizer solutions[J].Soil Science Society of America Journal,1999,55(6):1761-1766.
[32]Schomburg L.Dietary Selenium and Human Health[J].Nutrients,2017,9(1):22.
[33]Ilham I,Siddik M A,Fotedar R.Effects of Organic Selenium Supplementation on Growth,Accumulation,Haematology and Histopathology of Juvenile Barramundi(Lates calcarifer)Fed High Soybean Meal Diets[J].Biological Trace Element Research,2016,174(2):436-447.
[34]陈长兰,郇丰宁,孟雪莲,等.硒对人体的作用机理及科学补硒方法[J].辽宁大学学报:自然科学版,2016,43(2):155-168.
[35]杨会芳,梁新安,常介田,等.叶面喷施硒肥对不同蔬菜硒富集及产量的影响[J].北方园艺,2015(11):158-161.
[36]王晋民,赵之重,沈增基.叶面施硒对青花菜含硒量及产量与品质的影响[J].西北农林科技大学学报:自然科学版,2006,34(3):127-130.
[37]张明中.番茄施硒的生理和品质效应及分子调控研究[D].重庆:西南大学,2014:5-13.
[38]代惠萍,赵桦,贾根良,等.硒胁迫对紫花苜蓿硒积累及矿质元素吸收的效应[J].华北农学报,2017,32(02):226-231.
[39]郑晓翠,王海波,王孝娣,等.巨峰葡萄对硒元素的吸收运转规律[J].中国土壤与肥料,2016(04):128-132.
[40]张栋,史力超,冶军,等.滴灌条件下不同价态外源硒对水稻硒吸收及转运的影响[J].中国水稻科学,2017,31(01):65-71.
[41]殷金岩,耿增超,李致颖,等.硒肥对马铃薯硒素吸收、转化及产量、品质的影响[J].生态学报,2015,35(3):823-829.
[42]高祥芝,刘增超,王铜.补硒提高抗氧化能力对慢型克山病预后影响的随机化试验[J].中华地方病学杂志,2016,35(8):577-581.
[43]王婧,李海蓉,杨林生.西藏昌都地区环境硒分布特征及其与大骨节病的关系[J].地理研究,2017,36(2):383-390.
[2]杨琼,侯青叶,顾秋蓓,等.广西武鸣县典型土壤剖面Se的地球化学特征及其影响因素研究[J].现代地质,2016,30(2):455-462.
[3]高祥芝,刘增超,王铜.补硒提高抗氧化能力对慢型克山病预后影响的随机化试验[J].中华地方病学杂志,2016,35(8):577-581.
[4]王婧,李海蓉,杨林生.西藏昌都地区环境硒分布特征及其与大骨节病的关系[J].地理研究,2017,36(2):383-390.
[5]童建川.重庆紫色土区硒分布特征研究[J].西南师范大学学报:自然科学版,2016,41(03):170-175.
[6]Schomburg L.Dietary Selenium and Human Health[J].Nutrients,2017,9(1):22.
[7]Galinha C,Sánchez-Martínez M,Pacheco A M,et al.Characterization of selenium-enriched wheat by agronomic biofortification[J].Journal of Food Science&Technology,2015,52(7):4236-4245.
[8]陈长兰,郇丰宁,孟雪莲,等.硒对人体的作用机理及科学补硒方法[J].辽宁大学学报:自然科学版,2016,43(2):155-168.
[9]开建荣,王彩艳,李冬.农产品中硒形态研究进展分析[J].宁夏农林科技,2017,58(3):36-38.
[10]Longchamp M,Castrec-Rouelle M,Biron P,et al.Variations in the accumulation,localization and rate of metabolization of selenium in mature Zea mays plants supplied with selenite or selenate[J].Food Chemistry,2015,182:128-135.
[11]安梦鱼,张青,王煌平,等.土壤植物系统硒累积迁移的影响因素及调控[J].中国农学通报,2017,33(11):64-68.
[12]何锡文.富硒生姜生态种植技术[J].安徽农学通报,2016,22(7):49-50.
[13]曹德宾,王广来,张克著,等.富硒香菇栽培关键技术[J].蔬菜,2017(5):65-67.
[14]Wiesner-Reinhold M,Schreiner M,Baldermann S,et al.Mechanisms of Selenium Enrichment and Measurement in Brassicaceous Vegetables,and Their Application to Human Health[J].Frontiers in Plant Science,2017,8(1365):1365.
[15]Nawaz F,Ashraf M Y,Ahmad R,et al.Selenium(Se)Regulates Seedling Growth in Wheat under Drought Stress[J].Advances in Chemistry,2014,3:670-675.
[16]Queval G,Thominet D,Vanacker H,et al.H2O2-activated upregulation of glutathione in Arabidopsis involves induction of genes encoding enzymes involved in cysteine synthesis in the chloroplast[J].分子植物:英文版,2009,2(2):344.
[17]吴丽军,孙小凤,张荣,等.青海不同品种春油菜对硒的吸收、积累及分配规律[J].西北农业学报,2013,22(8):47-51.
[18]鲍士旦.土壤农化分析(第三版)[M].北京:中国农业出版社,2000:56-109,263-271.
[19]穆婷婷,杜慧玲,张福耀,等.外源硒对谷子生理特性、硒含量及其产量和品质的影响[J].中国农业科学,2017,50(1):51-63.
[20]Pasko P,Gdula-Argasinska J,Podporska-Carroll J,et al.Influence of selenium supplementation on fatty acids profile and biological activity of four edible amaranth sprouts as new kind of functional food[J].Journal of Food Science&Technology,2015,52(8):4724-4736.
[21]陈雪,沈方科,梁欢婷,等.外源施硒措施对水稻产量品质及植株硒分布的影响[J].南方农业学报,2017,48(1):46-50.
[22]梁春红,康云艳,柴喜荣,等.硒营养对菜心(菜薹)植株生长、产量和品质的影响[J].广东农业科学,2014,41(07):33-37.
[23]张庆社,闫妞,赵玉玲,等.叶面喷施外源硒对番茄果实品质的影响[J].现代农业科技,2017(10):66-67.
[24]李全,郑斌,刘丽,等.喷施有机硒肥对苹果果实硒含量及品质的影响[J].山西农业科学,2016,44(9):1316-1319.
[25]郝浩浩,汤曙光,刘星明,等.富硒樱桃生产技术试验研究[J].山西果树,2016(5):1-3.
[26]张杨杨,焦自高,艾希珍,等.土壤增施蛋氨酸硒对厚皮甜瓜生理特性和品质的影响[J].园艺学报,2015,42(1):1-11.
[27]胡秋辉,杨方美,潘根兴,等.喷施硒对大豆品质和大豆食品硒水平的影响[J].中国油料作物学报,2001,23(3):42-45.
[28]Lv J,Wu J,Zuo J,et al.Effect of Se treatment on the volatile compounds in broccoli[J].Food Chemistry,2017:216:225-233.
[29]陶爱芬,陈娴娴,祁建民,等.外源硒及脯氨酸对菜用黄麻生长和生理特性的影响[J].中国麻业科学,2015,37(05):239-245.
[30]张琳,孙炎鑫,廖上强.叶面喷施硒对生菜长势和品质的影响[J/OL].现代农业科技,2017(12):61-62.
[31]Al-Kanani T,MacKenzie A F,Barthakur N N.Soil-water and ammonia volatilization relationships with surface-applied nitrogenfertilizer solutions[J].Soil Science Society of America Journal,1999,55(6):1761-1766.
[32]Schomburg L.Dietary Selenium and Human Health[J].Nutrients,2017,9(1):22.
[33]Ilham I,Siddik M A,Fotedar R.Effects of Organic Selenium Supplementation on Growth,Accumulation,Haematology and Histopathology of Juvenile Barramundi(Lates calcarifer)Fed High Soybean Meal Diets[J].Biological Trace Element Research,2016,174(2):436-447.
[34]陈长兰,郇丰宁,孟雪莲,等.硒对人体的作用机理及科学补硒方法[J].辽宁大学学报:自然科学版,2016,43(2):155-168.
[35]杨会芳,梁新安,常介田,等.叶面喷施硒肥对不同蔬菜硒富集及产量的影响[J].北方园艺,2015(11):158-161.
[36]王晋民,赵之重,沈增基.叶面施硒对青花菜含硒量及产量与品质的影响[J].西北农林科技大学学报:自然科学版,2006,34(3):127-130.
[37]张明中.番茄施硒的生理和品质效应及分子调控研究[D].重庆:西南大学,2014:5-13.
[38]代惠萍,赵桦,贾根良,等.硒胁迫对紫花苜蓿硒积累及矿质元素吸收的效应[J].华北农学报,2017,32(02):226-231.
[39]郑晓翠,王海波,王孝娣,等.巨峰葡萄对硒元素的吸收运转规律[J].中国土壤与肥料,2016(04):128-132.
[40]张栋,史力超,冶军,等.滴灌条件下不同价态外源硒对水稻硒吸收及转运的影响[J].中国水稻科学,2017,31(01):65-71.
[41]殷金岩,耿增超,李致颖,等.硒肥对马铃薯硒素吸收、转化及产量、品质的影响[J].生态学报,2015,35(3):823-829.
[42]高祥芝,刘增超,王铜.补硒提高抗氧化能力对慢型克山病预后影响的随机化试验[J].中华地方病学杂志,2016,35(8):577-581.
[43]王婧,李海蓉,杨林生.西藏昌都地区环境硒分布特征及其与大骨节病的关系[J].地理研究,2017,36(2):383-390.