鹅源大肠杆菌的分离鉴定及致病性研究
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摘要
针对当前南方养鹅生产中大肠杆菌(Escherichia coli)病频发的问题,从省内规模化养鹅场收集病料,通过分离培养、生化鉴定、血清型鉴定分离不同血清型大肠杆菌,并在小鼠和雏鹅上开展攻毒试验.结果显示:成功分离得到O_(114)、O_(26)、O_(119)、O_(126)和O_(124)5个血清型大肠杆菌,且对小鼠和雏鹅均有不同程度致病性,其中O_(26)对小鼠致病性最强,死亡率达100%;O_(119)对小鹅致病性最强,达50%;O_(124)致病性最弱,均未使动物致死.
Escherichia coli was frequently encountered in the production of southern geese.Different serotypes of E.coli were isolated and cultured.Their biochemical features and serotype were determined.Their toxicities were tested on mice and gosling.The results showed that five serotypes were successfully separated.These serotypes were O_(114),O_(26),O_(119),O_(126),O_(124).They had different degrees of pathogenicity to both mice and goslings.Among them,O_(26)was the most pathogenic,with a mortality rate of 100%.O_(119)was the most pathogenic to the goose,reaching 50%.O_(124)was the least pathogenic and did not kill animals.
Escherichia coli was frequently encountered in the production of southern geese.Different serotypes of E.coli were isolated and cultured.Their biochemical features and serotype were determined.Their toxicities were tested on mice and gosling.The results showed that five serotypes were successfully separated.These serotypes were O_(114),O_(26),O_(119),O_(126),O_(124).They had different degrees of pathogenicity to both mice and goslings.Among them,O_(26)was the most pathogenic,with a mortality rate of 100%.O_(119)was the most pathogenic to the goose,reaching 50%.O_(124)was the least pathogenic and did not kill animals.
引文
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[19]植婵萍,贺丹丹,陈孝杰,等.种鹅大肠杆菌分离鉴定及耐药性分析中国畜牧兽医,2014,41(9):245-249.
[20]江丹莉,刘丽,陈芳,等.细菌内毒素对肉鹅生长性能及免疫机能的影响研究[J].中国家禽,2011,33(7):10-15.
[21]张童利,王曼宇,曹俊,等.鸡白痢沙门氏菌与其它致病性沙门氏菌的血清型特异性PCR鉴别检测方法的建立[J].中国预防兽医学报,2017,39(3):215-219.
[2]KUNERT F H C,CARVALHO D,GRASSOTTI T T,et al.Avian pathogenic Escherichia coli-methods for improved diagnosis[J].World's Poul Sci J,2015,71(2):249-258.
[3]伍莉,陈鹏飞.荣昌部分地区鸭源致病性大肠杆菌的分离及血清型鉴定[J].中国兽医杂志,2015,51(1):45-46.
[4]张力国,卢婷,赵明礼,等.牛源大肠杆菌毒力基因的检测及其对小鼠致病性的研究[J].中国兽医科学,2014,44(8):817-822.
[5]SOUZA A I,NETO O C,BATISTA D F,et al.ERIC-PCR genotyping of field isolates of Salmonella enterica subsp.enterica serovar Gallinarum biovars Gallinarum and Pullorum[J].Avian Pathol,2015,44(6):475-479.
[6]刘书宏,吴科敏,周辰瑜,等.食源性沙门氏菌分离株致病性试验和毒力相关基因检测[J/OL].食品科学(2017-09-17).http://www.spkx.net.cn/CN/article/download Article File.do?attach Type=PDF&id=42847.
[7]张丽芳,肖桥,罗薇.成都某种禽场鸡胚沙门氏菌的检测[J].中国畜牧兽医,2015,42(6):1571-1579.
[8]刘阳,吴白,张竹君,等.一株鹅源性鼠伤寒沙门氏菌的分离与鉴定[J].上海畜牧兽医通讯,2013(2):34-35.
[9]丛日超.山东地区病死鸭胚中细菌的分离鉴定及大肠杆菌分子流行病学调查[D].泰安:山东农业大学,2016.
[10]安微,张秀英,李蕊,等.致病性大肠杆菌毒力因子和耐药性研究进展[J].畜牧与兽医,2013,45(8):106-110.
[11]郭强强,孔静雅,柴迎锦,等.绵羊肺源性致病性大肠杆菌的分离与鉴定[J].微生物学通报,2017,44(12):2805-2811.
[12]李世静,嵇辛勤,文明,等.养鸭场主要细菌分离鉴定及致病性研究[J].中国畜牧兽医,2014,41(12):267-271.
[13]TU J,XUE T,QI Q,et al.The irp2 and fyu A genes in High Pathogennicity Islands are involved in the pathogenesis of infections caused by avian pathogenic Escherichia coli(APEC)[J].Polish J Veter Sci,2016,19(1):21-29.
[14]南文金,黄健强,吴静波,等.粤北地区仔猪腹泻源大肠杆菌毒力基因检测及致病性初步研究[J].广东农业科学,2017,44(6):129-134.
[15]ZICAVO A.Prevalenc and characteristics of verotoxigenic Escherichia coli strains isolated from pigs pork products in Umbria and Marche regions of Italy[J].J Food Prot,2015,78(4):796-801.
[16]ARISOY M,RAD A Y,AKIN A,et,al.Relationship between susceptibility to antimicrobials and virulence factors in paediatric Escherichia coli isolates[J].Int J Ant Agents,2008,31(1):4-8.
[17]侍亚,宋桂芬,张莲,等.盐城地区鹅源大肠杆菌的致病性试验[J].养禽与禽病防治,2010(2):7-8.
[18]宦海霞,苗晓青,周琼,等.江苏省部分地区鹅源致病性大肠杆菌的分离、鉴定和耐药性分析[J].饲料工业,2007,28(15):62-64.
[19]植婵萍,贺丹丹,陈孝杰,等.种鹅大肠杆菌分离鉴定及耐药性分析中国畜牧兽医,2014,41(9):245-249.
[20]江丹莉,刘丽,陈芳,等.细菌内毒素对肉鹅生长性能及免疫机能的影响研究[J].中国家禽,2011,33(7):10-15.
[21]张童利,王曼宇,曹俊,等.鸡白痢沙门氏菌与其它致病性沙门氏菌的血清型特异性PCR鉴别检测方法的建立[J].中国预防兽医学报,2017,39(3):215-219.