响应面法优化栀子油提取工艺研究
|
摘要
以石油醚为提取溶剂,研究溶剂法提取栀子油工艺。选取提取时间、提取温度、料液比作为影响因素,在单因素试验的基础上,以栀子油得率为响应值,进行三因素三水平的Box-Behnken试验,采用响应面法优化栀子油的提取工艺。结果表明:栀子油提取最佳工艺条件为提取时间4 h、提取温度81℃、料液比1∶5。在最佳工艺条件下,栀子油得率达到19.21%,与模型预测值(19.37%)接近,说明所得优化条件可行。
With petroleum ether as extraction solvent,the extraction process of Gardenia oil was studied by solvent method.The extraction time,extraction temperature and ratio of material to liquid were selected as influencing factors.On the basis of single factor experiment,the Box-Behnken experiment of three factors and three levels was carried out and the extraction process of Gardenia oil was optimized by response surface methodology.The results showed that the optimal extraction conditions of Gardenia oil were obtained as follows:extraction time 4 h,extraction temperature 81℃,and ratio of material to liquid 1∶5.Under these conditions,the yield of Gardenia oil reached 19.21%,which was close to the predictive value(19.37%),indicating that the optimal conditions obtained were feasible.
With petroleum ether as extraction solvent,the extraction process of Gardenia oil was studied by solvent method.The extraction time,extraction temperature and ratio of material to liquid were selected as influencing factors.On the basis of single factor experiment,the Box-Behnken experiment of three factors and three levels was carried out and the extraction process of Gardenia oil was optimized by response surface methodology.The results showed that the optimal extraction conditions of Gardenia oil were obtained as follows:extraction time 4 h,extraction temperature 81℃,and ratio of material to liquid 1∶5.Under these conditions,the yield of Gardenia oil reached 19.21%,which was close to the predictive value(19.37%),indicating that the optimal conditions obtained were feasible.
引文
[1]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:248.
[2]廖夫生.中药栀子研究进展[J].广州化工,2013,41(1):12-13,21.
[3]杨冀艳,许世晓,胡磊.超声波辅助提取栀子油及其脂肪酸组成分析研究[J].食品科学,2008,29(11):246-249.
[4]李昊阳,王飞运,刘华敏,等.不同方法制备的栀子果油的理化性质比较[J].现代食品科技,2016,32(9):209-215.
[5]张风波,罗光明,肖日传,等.栀子油对四氯化碳所致小鼠急性肝损伤的保护作用[J].中国油脂,2017,42(7):128-131.
[6]李宝莉,陈雅慧,杨暄,等.栀子油的提取和对中枢神经系统的作用[J].第四军医大学学报,2008,29(23):2152-2155.
[7]刘继平,许海,胡锐,等.栀子油对S180荷瘤小鼠肿瘤生长及胸腺、脾指数的影响[J].西北药学杂志,2010,25(2):112-114.
[8]李宝莉,陈雅慧,胡锐,等.复方酸枣仁油栀子油对小鼠的镇静、催眠和抗惊厥作用[J].南方医科大学学报,2008,28(9):1636-1639.
[9]包亚妮,董建青,贺文浩,等.二次通用旋转组合设计法优化超临界CO2萃取栀子油的工艺研究[J].中国粮油学报,2011,26(5):66-70,75.
[10]袁源见,罗光明,魏春华,等.响应面优化超声波提取栀子油脂工艺研究[J].世界科学技术:中医药现代化,2016,18(7):1206-1211.
[11]龙云飞,王沛佩,杨克迪,等.响应面法优化亚麻籽油提取工艺[J].食品科学,2012,33(14):45-49.
[12]王鸿,邓泽元,刘蓉,等.响应曲面法优化山蕗菜根多糖的提取工艺[J].食品科学,2010,31(2):46-50.
[13]姚笛,马萍,王颖,等.响应面法优化玉米芯中木聚糖的提取工艺[J].食品科学,2011,32(8):111-115.
[14]李静,姚茂君,李俊,等.响应面法优化牡丹籽油的水酶法提取工艺[J].中国油脂,2014,39(10):14-18.
[15]韩雅珊.食品化学实验指导[M].北京:中国农业大学出版社,1992:25-27.
[2]廖夫生.中药栀子研究进展[J].广州化工,2013,41(1):12-13,21.
[3]杨冀艳,许世晓,胡磊.超声波辅助提取栀子油及其脂肪酸组成分析研究[J].食品科学,2008,29(11):246-249.
[4]李昊阳,王飞运,刘华敏,等.不同方法制备的栀子果油的理化性质比较[J].现代食品科技,2016,32(9):209-215.
[5]张风波,罗光明,肖日传,等.栀子油对四氯化碳所致小鼠急性肝损伤的保护作用[J].中国油脂,2017,42(7):128-131.
[6]李宝莉,陈雅慧,杨暄,等.栀子油的提取和对中枢神经系统的作用[J].第四军医大学学报,2008,29(23):2152-2155.
[7]刘继平,许海,胡锐,等.栀子油对S180荷瘤小鼠肿瘤生长及胸腺、脾指数的影响[J].西北药学杂志,2010,25(2):112-114.
[8]李宝莉,陈雅慧,胡锐,等.复方酸枣仁油栀子油对小鼠的镇静、催眠和抗惊厥作用[J].南方医科大学学报,2008,28(9):1636-1639.
[9]包亚妮,董建青,贺文浩,等.二次通用旋转组合设计法优化超临界CO2萃取栀子油的工艺研究[J].中国粮油学报,2011,26(5):66-70,75.
[10]袁源见,罗光明,魏春华,等.响应面优化超声波提取栀子油脂工艺研究[J].世界科学技术:中医药现代化,2016,18(7):1206-1211.
[11]龙云飞,王沛佩,杨克迪,等.响应面法优化亚麻籽油提取工艺[J].食品科学,2012,33(14):45-49.
[12]王鸿,邓泽元,刘蓉,等.响应曲面法优化山蕗菜根多糖的提取工艺[J].食品科学,2010,31(2):46-50.
[13]姚笛,马萍,王颖,等.响应面法优化玉米芯中木聚糖的提取工艺[J].食品科学,2011,32(8):111-115.
[14]李静,姚茂君,李俊,等.响应面法优化牡丹籽油的水酶法提取工艺[J].中国油脂,2014,39(10):14-18.
[15]韩雅珊.食品化学实验指导[M].北京:中国农业大学出版社,1992:25-27.