高效液相色谱-蒸发光散射法测定栀子油中的5种高级脂肪酸
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摘要
目的:建立HPLC-ELSD法分析栀子油中亚麻酸、亚油酸、棕榈酸、油酸和硬脂酸5种高级脂肪酸,并测定其含量。方法:色谱柱Agilent ZORBAX XDB-C18(4.6mm×250mm,5μm),流动相:甲醇-1%乙酸水溶液梯度洗脱,流速:1m L/min,柱温:25℃,ELSD参数:载气:氮气,压力:40Psi,漂移管温度70℃,增益:200,进样量:10μL。结果:亚麻酸、亚油酸、棕榈酸、油酸和硬脂酸的回收率均位于为98.5%~100.5%,相对标准偏差(n=6)分别为1.6%、1.2%、0.8%、1.6%、1.1%。结论:该方法简捷、精确、重复性好,适合栀子油中5种高含量脂肪酸成分的含量测定。
Objective: To establish HPLC-ELSD method for analyzing 5 fatty acids in gardenia oil, including linolenic acid, linoleic acid, palmitic acid, oleic acid and stearic acid, and measure the content. Methods: The Agilent ZORBAX XDB-C18 analytical column(4.6 mm×250 mm, 5μm) with methanol-acetic acid aqueous solution as mobile phase at a flow rate of 1.0 m L/min were applied. The column temperature was set at 25℃. The parameters of ELSD were as follows: the carrier gas pressure was 40 Psi, the temperature of the drift tube was 70℃, the plus was 200, sample size was 10μL. Results: The recoveries of linolenic acid, linoleic acid, palmitic acid, oleic acid and stearic acid were all located at 98.5% to 100.5%, and the relative standard deviation(n=6) was 1.6%, 1.2%, 0.8%, 1.6%, 1.1%. Conclusion: This method is simple, accurated and reproducible, it is suitable for the simultaneous determination of the five kinds of high fatty acids in gardenia oil.
Objective: To establish HPLC-ELSD method for analyzing 5 fatty acids in gardenia oil, including linolenic acid, linoleic acid, palmitic acid, oleic acid and stearic acid, and measure the content. Methods: The Agilent ZORBAX XDB-C18 analytical column(4.6 mm×250 mm, 5μm) with methanol-acetic acid aqueous solution as mobile phase at a flow rate of 1.0 m L/min were applied. The column temperature was set at 25℃. The parameters of ELSD were as follows: the carrier gas pressure was 40 Psi, the temperature of the drift tube was 70℃, the plus was 200, sample size was 10μL. Results: The recoveries of linolenic acid, linoleic acid, palmitic acid, oleic acid and stearic acid were all located at 98.5% to 100.5%, and the relative standard deviation(n=6) was 1.6%, 1.2%, 0.8%, 1.6%, 1.1%. Conclusion: This method is simple, accurated and reproducible, it is suitable for the simultaneous determination of the five kinds of high fatty acids in gardenia oil.
引文
[1]李昊阳,王飞运,刘华敏,等.不同方法制备的栀子果油的理化性质比较.现代食品科技,2016,32(9):209-215
[2]杨冀艳,许世晓,胡磊.超声波辅助提取栀子油及其脂肪酸组成分析研究.食品科学,2008,29(11):246-249
[3]甘辉,万明,石小凤,等.栀子果油的脂肪酸组成及其主要理化性质分析.江西食品工业,2009,28(3):24-25
[4]包亚妮,董建青,袁芳.超临界CO2萃取工艺条件对栀子油脂肪酸组成及其抗氧化活性的影响.食品科学,2011,32(10):12-17
[5]李宝莉,陈雅慧,杨暄,等.栀子油的提取和对中枢神经系统的作用.第四军医大学学报,2008,29(23):2152-2155
[6]刘继平,许海,胡锐,等.栀子油对S180荷瘤小鼠肿瘤生长及胸腺、脾指数的影响.西北药学杂志,2010,25(2):112-114
[7]刘涛,尹春华,谭天伟.高效液相色谱-蒸发光散射检测器测定脂肪酸和甘油酯的含量.中国油脂,2005,30(9):50-53
[8]史晓凡,周欣欣,王磊,等.高效液相色谱-蒸发光散射法分析人和动物油脂种属.中国法医学杂志,2013,28(2):116-118
[9]谢元勋,何厚洪,盛卫国,等.HPLC-ELSD测定油菜蜂花粉中6种脂肪酸.中国现代应用药学,2014,31(5):559-563
[10]刘江生,张鼎方,赖伟玲,等.高效液相色谱-蒸发光散射法测定烟草中的高级脂肪酸.烟草科技,2011,284(3):55-58
[11]盛孝杰.牡丹籽油中脂肪酸的超高效液相色谱新分析方法研究.长沙:中南林业科技大学,2015
[12]葛姗姗,施思,郭鑫艳,等.HPLC-ELSD法测定红腺忍冬叶抗氧化有效部位中果糖和葡萄糖的含量.中华中医药学刊,2014(4):882-885
[13]范奎,代良敏,熊永爱,等.HPLC-ELSD法测定地榆总皂苷中地榆皂苷-Ⅰ和地榆皂苷-Ⅱ的含量.中华中医药杂志,2017,32(6):2639-2642
[2]杨冀艳,许世晓,胡磊.超声波辅助提取栀子油及其脂肪酸组成分析研究.食品科学,2008,29(11):246-249
[3]甘辉,万明,石小凤,等.栀子果油的脂肪酸组成及其主要理化性质分析.江西食品工业,2009,28(3):24-25
[4]包亚妮,董建青,袁芳.超临界CO2萃取工艺条件对栀子油脂肪酸组成及其抗氧化活性的影响.食品科学,2011,32(10):12-17
[5]李宝莉,陈雅慧,杨暄,等.栀子油的提取和对中枢神经系统的作用.第四军医大学学报,2008,29(23):2152-2155
[6]刘继平,许海,胡锐,等.栀子油对S180荷瘤小鼠肿瘤生长及胸腺、脾指数的影响.西北药学杂志,2010,25(2):112-114
[7]刘涛,尹春华,谭天伟.高效液相色谱-蒸发光散射检测器测定脂肪酸和甘油酯的含量.中国油脂,2005,30(9):50-53
[8]史晓凡,周欣欣,王磊,等.高效液相色谱-蒸发光散射法分析人和动物油脂种属.中国法医学杂志,2013,28(2):116-118
[9]谢元勋,何厚洪,盛卫国,等.HPLC-ELSD测定油菜蜂花粉中6种脂肪酸.中国现代应用药学,2014,31(5):559-563
[10]刘江生,张鼎方,赖伟玲,等.高效液相色谱-蒸发光散射法测定烟草中的高级脂肪酸.烟草科技,2011,284(3):55-58
[11]盛孝杰.牡丹籽油中脂肪酸的超高效液相色谱新分析方法研究.长沙:中南林业科技大学,2015
[12]葛姗姗,施思,郭鑫艳,等.HPLC-ELSD法测定红腺忍冬叶抗氧化有效部位中果糖和葡萄糖的含量.中华中医药学刊,2014(4):882-885
[13]范奎,代良敏,熊永爱,等.HPLC-ELSD法测定地榆总皂苷中地榆皂苷-Ⅰ和地榆皂苷-Ⅱ的含量.中华中医药杂志,2017,32(6):2639-2642