乳酸链球菌素的特性及抗菌材料在口腔中应用的研究进展
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摘要
致龋菌对树脂粘接材料的黏附及其在牙体组织表面产酸可引起继发龋,最终导致牙体缺损修复的失败。乳酸链球菌素(nisin)作为乳酸链球菌产生的一种3.4 k Da的抗菌肽,通过在细菌的细胞膜上产生孔道杀菌,对口腔致龋菌有明显抑制作用,具有应用于抗菌材料防止继发龋的潜能。本文就nisin的特性及其口腔抗菌材料中的应用研究进展作一综述。
The cariogenic bacteria adhesion to the resin bonding material and acid metabolites on the surface the dental tissue can cause secondary caries,andresult in the failure of restorations. Nisin is a3.4 k Da antimicrobial peptide that kill the bacteria by pores on the surface of the cell membrane. It has significant inhibitory effect of cariogenic bacteria,and is considered to apply in antibacterial materials to prevent secondary caries. This review is to summarize the characteristics of nisin and its latest developments in dental field.
The cariogenic bacteria adhesion to the resin bonding material and acid metabolites on the surface the dental tissue can cause secondary caries,andresult in the failure of restorations. Nisin is a3.4 k Da antimicrobial peptide that kill the bacteria by pores on the surface of the cell membrane. It has significant inhibitory effect of cariogenic bacteria,and is considered to apply in antibacterial materials to prevent secondary caries. This review is to summarize the characteristics of nisin and its latest developments in dental field.
引文
[1]冯希平.牙体充填后继发龋的产生和预防[J].华西口腔医学杂志,2014,32(2):107-110.
[2]Bourbia M,Ma D,Cvitkovitch DG,et al.Cariogenic bacteria degrade dental resin composites and adhesives[J].J Dent Res,2013,92(11):989-994.
[3]Buchman GW,Banerjee S,Hansen JN.Structure,expression,and evolution of a gene encoding the precursor of nisin,a small protein antibiotic[J].J Biol Chem,1988,263(31):16260-16266.
[4]Rollema HS,Metzger JW,Both P,et al.Structure and biological activity of chemically modified nisin A species[J].Eur J Biochem,1996,241(3):716-722.
[5]Mulders JW,Boerrigter IJ,Rollema HS,et al.Identification and characterization of the lantibiotic nisin Z,a natural nisin variant[J].Eur J Biochem,1991,201(3):581-584.
[6]de Vos WM,Mulders JW,Siezen RJ,et al.Properties of nisin Z and distribution of its gene,nis Z,in Lactococcus lactis[J].Appl Environ Microbiol,1993,59(1):213-218.
[7]Rollema HS,Kuipers OP,Both P,et al.Improvement of solubility and stability of the antimicrobial peptide nisin by protein engineering[J].Appl Environ Microbiol,1995,61(8):2873-2878.
[8]Taylor TM,Davidson PM,Zhong Q.Extraction of nisin from a2.5%commercial nisin product using methanol and ethanol solutions[J].J Food Prot,2007,70(5):1272-1276.
[9]Abee T,Krockel L,Hill C.Bacteriocins:modes of action and potentials in food preservation and control of food poisoning[J].Int J Food Microbiol,1995,28(2):169-185.
[10]Ukuku DO,Shelef LA.Bioluminescence measurements of the antilisterial activity of nisin:comparison with ampicillin and streptomycin[J].J Biolumin Chemilumin,1996,11(3):169-173.
[11]Bauer R,Dicks LM.Mode of action of lipidⅡ-targeting lantibiotics[J].Int J Food Microbiol,2005,101(2):201-216.
[12]Thomas LV,Wimpenny JW.Investigation of the effect of combined variations in temperature,p H,and Na Cl concentration on nisin inhibition of Listeria monocytogenes and Staphylococcus aureus[J].Appl Environ Microbiol,1996,62(6):2006-2012.
[13]刘丽莉,马美湖,杨协力.非生物性因素对乳酸链球菌素抗菌活性的影响[J].食品科学,2009,30(5):46-49.
[14]路遥,蒋立科,陈美玲,等.通过定点突变提高乳链菌肽对热及p H的稳定性[J].微生物学报,2010,50(11):1481-1487.
[15]Syafiuddin T,Hisamitsu H,Toko T,et al.In vitro inhibition of caries around a resin composite restoration containing antibacterial filler[J].Biomaterials,1997,18(15):1051-1057.
[16]Jedrychowski JR,Caputo AA,Kerper S.Antibacterial and mechanical properties of restorative materials combined with chlorhexidines[J].J Oral Rehabil,1983,10(5):373-381.
[17]Elsaka SE.Antibacterial activity and adhesive properties of a chitosan-containing dental adhesive[J].Quintessence Int,2012,43(7):603-613.
[18]Li F,Chen J,Chai Z,et al.Effects of a dental adhesive incorporating antibacterial monomer on the growth,adherence and membrane integrity of Streptococcus mutans[J].J Dent,2009,37(4):289-296.
[19]Zhang K,Cheng L,Imazato S,et al.Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm,cytotoxicity and dentine bond properties[J].J Dent,2013,41(5):464-474.
[20]Melo MA,Cheng L,Weir MD,et al.Novel dental adhesive containing antibacterial agents and calcium phosphate nanoparticles[J].J Biomed Mater Res B Appl Biomater,2013,101(4):620-629.
[21]Zhou H,Li F,Weir MD,et al.Dental plaque microcosm response to bonding agents containing quaternary ammonium methacrylates with different chain lengths and charge densities[J].J Dent,2013,41(11):1122-1131.
[22]Li F,Weir MD,Fouad AF,et al.Effect of salivary pellicle on antibacterial activity of novel antibacterial dental adhesives using a dental plaque microcosm biofilm model[J].Dent Mater,2014,30(2):182-191.
[23]Brambilla E,Ionescu A,Fadini L,et al.Influence of MDPBcontaining primer on Streptococcus mutans biofilm formation in simulated ClassⅠrestorations[J].J Adhes Dent,2013,15(5):431-438.
[24]LaukováA,ChrastinováL,PlacháI,et al.Beneficial effect of lantibiotic nisin in rabbit husbandry[J].Probiotics Antimicrob Proteins,2014,6(1):41-46.
[25]Johnson IH,Hayday H,Colman G.The effects of nisin on the microbial flora of the dental plaque of monkeys(Macaca fascicularis)[J].J Appl Bacteriol,1978,45(1):99-109.
[26]Howell TH,Fiorellini JP,Blackburn P,et al.The effect of a mouthrinse based on nisin,a bacteriocin,on developing plaque and gingivitis in beagle dogs[J].J Clin Periodontol,1993,20(5):335-339.
[27]Tong Z,Dong L,Zhou L,et al.Nisin inhibits dental cariesassociated microorganism in vitro[J].Peptides,2010,31(11):2003-2008.
[28]Tong Z,Zhou L,Jiang W,et al.An in vitro synergetic evaluation of the use of nisin and sodium fluoride or chlorhexidine against Streptococcus mutans[J].Peptides,2011,32(10):2021-2026.
[29]Shin JM,Ateia I,Paulus JR,et al.Antimicrobial nisin acts against saliva derived multi-species biofilms without cytotoxicity to human oral cells[J].Front Microbiol,2015(6):617.
[30]Turner SR,Love RM,Lyons KM.An in-vitro investigation of the antibacterial effect of nisin in root canals and canal wall radicular dentine[J].Int Endod J,2004,37(10):664-671.
[31]Tong Z,Zhang Y,Ling J,et al.An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis[J].PLo S One,2014,9(2):e89209.
[32]Tong Z,Zhou L,Kuang R,et al.In vitro evaluation of MTAD and nisin in combination against common pathogens associated with root canal infection[J].J Endod,2012,38(4):490-494.
[33]Balto HA,Shakoor ZA,Kanfar MA.Combined effect of a mixture of tetracycline,acid,and detergent,and nisin against Enterococcus faecalis and Actinomyces viscosus biofilms[J].Saudi Med J,2015,36(2):211-215.
[34]Yamakami K,Tsumori H,Sakurai Y,et al.Sustainable inhibition efficacy of liposome-encapsulated nisin on insoluble glucanbiofilm synthesis by Streptococcus mutans[J].Pharm Biol,2013,51(2):267-270.
[2]Bourbia M,Ma D,Cvitkovitch DG,et al.Cariogenic bacteria degrade dental resin composites and adhesives[J].J Dent Res,2013,92(11):989-994.
[3]Buchman GW,Banerjee S,Hansen JN.Structure,expression,and evolution of a gene encoding the precursor of nisin,a small protein antibiotic[J].J Biol Chem,1988,263(31):16260-16266.
[4]Rollema HS,Metzger JW,Both P,et al.Structure and biological activity of chemically modified nisin A species[J].Eur J Biochem,1996,241(3):716-722.
[5]Mulders JW,Boerrigter IJ,Rollema HS,et al.Identification and characterization of the lantibiotic nisin Z,a natural nisin variant[J].Eur J Biochem,1991,201(3):581-584.
[6]de Vos WM,Mulders JW,Siezen RJ,et al.Properties of nisin Z and distribution of its gene,nis Z,in Lactococcus lactis[J].Appl Environ Microbiol,1993,59(1):213-218.
[7]Rollema HS,Kuipers OP,Both P,et al.Improvement of solubility and stability of the antimicrobial peptide nisin by protein engineering[J].Appl Environ Microbiol,1995,61(8):2873-2878.
[8]Taylor TM,Davidson PM,Zhong Q.Extraction of nisin from a2.5%commercial nisin product using methanol and ethanol solutions[J].J Food Prot,2007,70(5):1272-1276.
[9]Abee T,Krockel L,Hill C.Bacteriocins:modes of action and potentials in food preservation and control of food poisoning[J].Int J Food Microbiol,1995,28(2):169-185.
[10]Ukuku DO,Shelef LA.Bioluminescence measurements of the antilisterial activity of nisin:comparison with ampicillin and streptomycin[J].J Biolumin Chemilumin,1996,11(3):169-173.
[11]Bauer R,Dicks LM.Mode of action of lipidⅡ-targeting lantibiotics[J].Int J Food Microbiol,2005,101(2):201-216.
[12]Thomas LV,Wimpenny JW.Investigation of the effect of combined variations in temperature,p H,and Na Cl concentration on nisin inhibition of Listeria monocytogenes and Staphylococcus aureus[J].Appl Environ Microbiol,1996,62(6):2006-2012.
[13]刘丽莉,马美湖,杨协力.非生物性因素对乳酸链球菌素抗菌活性的影响[J].食品科学,2009,30(5):46-49.
[14]路遥,蒋立科,陈美玲,等.通过定点突变提高乳链菌肽对热及p H的稳定性[J].微生物学报,2010,50(11):1481-1487.
[15]Syafiuddin T,Hisamitsu H,Toko T,et al.In vitro inhibition of caries around a resin composite restoration containing antibacterial filler[J].Biomaterials,1997,18(15):1051-1057.
[16]Jedrychowski JR,Caputo AA,Kerper S.Antibacterial and mechanical properties of restorative materials combined with chlorhexidines[J].J Oral Rehabil,1983,10(5):373-381.
[17]Elsaka SE.Antibacterial activity and adhesive properties of a chitosan-containing dental adhesive[J].Quintessence Int,2012,43(7):603-613.
[18]Li F,Chen J,Chai Z,et al.Effects of a dental adhesive incorporating antibacterial monomer on the growth,adherence and membrane integrity of Streptococcus mutans[J].J Dent,2009,37(4):289-296.
[19]Zhang K,Cheng L,Imazato S,et al.Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm,cytotoxicity and dentine bond properties[J].J Dent,2013,41(5):464-474.
[20]Melo MA,Cheng L,Weir MD,et al.Novel dental adhesive containing antibacterial agents and calcium phosphate nanoparticles[J].J Biomed Mater Res B Appl Biomater,2013,101(4):620-629.
[21]Zhou H,Li F,Weir MD,et al.Dental plaque microcosm response to bonding agents containing quaternary ammonium methacrylates with different chain lengths and charge densities[J].J Dent,2013,41(11):1122-1131.
[22]Li F,Weir MD,Fouad AF,et al.Effect of salivary pellicle on antibacterial activity of novel antibacterial dental adhesives using a dental plaque microcosm biofilm model[J].Dent Mater,2014,30(2):182-191.
[23]Brambilla E,Ionescu A,Fadini L,et al.Influence of MDPBcontaining primer on Streptococcus mutans biofilm formation in simulated ClassⅠrestorations[J].J Adhes Dent,2013,15(5):431-438.
[24]LaukováA,ChrastinováL,PlacháI,et al.Beneficial effect of lantibiotic nisin in rabbit husbandry[J].Probiotics Antimicrob Proteins,2014,6(1):41-46.
[25]Johnson IH,Hayday H,Colman G.The effects of nisin on the microbial flora of the dental plaque of monkeys(Macaca fascicularis)[J].J Appl Bacteriol,1978,45(1):99-109.
[26]Howell TH,Fiorellini JP,Blackburn P,et al.The effect of a mouthrinse based on nisin,a bacteriocin,on developing plaque and gingivitis in beagle dogs[J].J Clin Periodontol,1993,20(5):335-339.
[27]Tong Z,Dong L,Zhou L,et al.Nisin inhibits dental cariesassociated microorganism in vitro[J].Peptides,2010,31(11):2003-2008.
[28]Tong Z,Zhou L,Jiang W,et al.An in vitro synergetic evaluation of the use of nisin and sodium fluoride or chlorhexidine against Streptococcus mutans[J].Peptides,2011,32(10):2021-2026.
[29]Shin JM,Ateia I,Paulus JR,et al.Antimicrobial nisin acts against saliva derived multi-species biofilms without cytotoxicity to human oral cells[J].Front Microbiol,2015(6):617.
[30]Turner SR,Love RM,Lyons KM.An in-vitro investigation of the antibacterial effect of nisin in root canals and canal wall radicular dentine[J].Int Endod J,2004,37(10):664-671.
[31]Tong Z,Zhang Y,Ling J,et al.An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis[J].PLo S One,2014,9(2):e89209.
[32]Tong Z,Zhou L,Kuang R,et al.In vitro evaluation of MTAD and nisin in combination against common pathogens associated with root canal infection[J].J Endod,2012,38(4):490-494.
[33]Balto HA,Shakoor ZA,Kanfar MA.Combined effect of a mixture of tetracycline,acid,and detergent,and nisin against Enterococcus faecalis and Actinomyces viscosus biofilms[J].Saudi Med J,2015,36(2):211-215.
[34]Yamakami K,Tsumori H,Sakurai Y,et al.Sustainable inhibition efficacy of liposome-encapsulated nisin on insoluble glucanbiofilm synthesis by Streptococcus mutans[J].Pharm Biol,2013,51(2):267-270.