朱红栓菌菌丝体的化学成分研究
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摘要
目的:明确朱红栓菌菌丝体的主要化学成分。方法:通过100 L发酵罐发酵培养朱红栓菌菌丝体,烘干粉碎后用乙酸乙酯提取,提取物采用硅胶和凝胶等柱色谱方法进行分离纯化,获得的单体化合物利用核磁共振波谱、质谱等技术进行结构鉴定。结果:从朱红栓菌菌丝体的乙酸乙酯提取物共分离纯化得到11个化合物,分别是1,3-二油酰甘油酯(1)、α,α'-双棕榈酰甘油酯(2)、麦角甾醇(3)、邻苯二甲酸二丁酯(4)、啤酒甾醇(5)、阿拉伯糖醇(6)、桦褐孔菌二糖(7)、尿嘧啶(8)、金雀异黄酮(9)、琥珀酸(10)、油酸(11)。结论:以上化合物均为首次从朱红栓菌菌丝体中分离得到。
Objective: To study the chemical constituents of mycelium of Trametes cinnabarina by liquid fermentation. Methods: Liquid fermentation was performed in 100 L fermentor. Mycelium of T. cinnabarina was extracted by ethyl acetate. Chemical constituents were isolated and purified using silica gel and gel column chromatographies. MS and NMR spectral analysis were used to identify their structures. Results: Eleven compounds were isolated from mycelium of T. cinnabarina and identified as 1,3-diolein( 1),plamitic acid-α,α'-dipropyl ester( 2),ergosterol( 3),dibutylphthalate( 4),cerevisterol( 5),arabitol( 6),inotodisaccharide( 7),uridine( 8),genistein( 9),succinic acid( 10),and oleic acid( 11). Conclusion: All the compounds were isolated from mycelium of T. cinnabarina for the first time.
Objective: To study the chemical constituents of mycelium of Trametes cinnabarina by liquid fermentation. Methods: Liquid fermentation was performed in 100 L fermentor. Mycelium of T. cinnabarina was extracted by ethyl acetate. Chemical constituents were isolated and purified using silica gel and gel column chromatographies. MS and NMR spectral analysis were used to identify their structures. Results: Eleven compounds were isolated from mycelium of T. cinnabarina and identified as 1,3-diolein( 1),plamitic acid-α,α'-dipropyl ester( 2),ergosterol( 3),dibutylphthalate( 4),cerevisterol( 5),arabitol( 6),inotodisaccharide( 7),uridine( 8),genistein( 9),succinic acid( 10),and oleic acid( 11). Conclusion: All the compounds were isolated from mycelium of T. cinnabarina for the first time.
引文
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[3]戴玉成,图力古尔.中国东北野生食药用真菌图志[M].北京:科学出版社,2007:107-108.
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[6]朱峰,卢卫红,陈忻,等.朱红栓菌中两个吩嗪酮类生物碱的分离与鉴定[J].天然产物研究与开发,2014,26(3):358-360.
[7]刘学湘,潘扬,蒋亚平.红栓菌液态发酵后马钱子总碱中2种生物碱含量的变化[J].安徽农业科学,2012,40(22):11225-11226.
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[10]刘云海,吴晓云,方建国,等.板蓝根化学成分研究(V)[J].中南药学,2003(5):302-305.
[11]吕子明,姜永涛,吴立军,等.人工蛹虫草子实体化学成分研究[J].中国中药杂志,2008,33(24):2914-2917.
[12]严振,田洋,马跃平,等.雷公藤根化学成分研究[J].中国现代中药,2010,12(1):23-24.
[13]刘玉红,徐凌川,王建平,等.茶藨子叶孔菌化学成分的研究[J].中药材,2005,28(11):998-999.
[14]王春兰,张集慧,郭顺星,等.粉红粘帚霉化学成分的研究[J].微生物学通报,2001,28(4):24-27.
[15]王宁辉.白檀化学成分及其生物活性研究[D].西安:陕西科技大学,2015.
[16]赵余庆,李铣.红毛五加化学成分的研究[J].中国中药杂志,1991,16(7):421-424.
[17]Agrawal P K.Carbon-13 NMR of flavonoids[J].Studies in Organic Chemistry,1989,39:283.
[18]黄文强,施敏峰,宋晓平,等.使君子化学成分研究[J].西北农林科技大学学报(自然科学版),2006,34(4):79-82.
[19]姬志强,唐娜娜,张建民,等.山风化学成分研究[J].中国药师,2015,18(12):2067-2069.