鲫鱼冬瓜皮汤改善肾病综合征大鼠肾功能的实验研究
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摘要
目的观察鲫鱼冬瓜皮汤联合常规治疗方法对肾病综合征模型大鼠肾功能的保护作用。方法将96只SD大鼠随机分为4组,分别为正常组、模型组、治疗组、对照组,对照组予以常规治疗,治疗组除常规治疗外,按照临床等效剂量予以鲫鱼冬瓜皮汤,连续14 d为一疗程,共治疗2个疗程。分别于治疗前、治疗2周及治疗4周后,检测大鼠肾组织病理形态学、24 h尿蛋白及肌酐、尿素氮、尿酸水平。结果与治疗前相比,对照组、治疗组体质量差异有统计学意义(P<0.05);与模型组比较,对照组与治疗组的体质量差异有统计学意义(P<0.05),但对照组和治疗组相比,体质量无明显差异(P>0.05)。与模型组相比较,对照组远端小管排列较紧密,组织间可见少量炎性渗出;治疗组较对照组减轻。经过4周的治疗后,治疗组尿蛋白、肌酐、尿素氮、尿酸水平较治疗前显著下降(P<0.05);与模型组比,治疗组指标有显著性改善(P<0.05);治疗组与对照组比较差异具有统计学意义(P<0.05)。结论采用鲫鱼冬瓜皮汤联合常规治疗方法可有效改善肾病综合征模型大鼠体质量比,减少其24 h尿蛋白及肌酐、尿素氮、尿酸水平。
Objective To observe the protective effect of Crucian Carp and Chinese Wax Gourd Peel Soup combined with routine treatment on renal function in nephrotic syndrome rats. Methods A total of 96 SD rats were randomly divided into4 groups: a normal group, a model group, a treatment group and a control group. The control group was given routine treatment.Except the routine treatment, the treatment group was also given Crucian Carp and Chinese Wax Gourd Peel Soup according to the clinical equivalent dosage. Continuous 14 d as 1 course of treatment, with a total of 2 courses of treatment. The pathomorphology of renal tissue, and the levels of 24 h urinary protein, creatinine, urea nitrogen and uric acid were measured before the treatment, 2 weeks after the treatment and 4 weeks after the treatment. Results Compared with the body mass before the treatment,the difference between the control group and the treatment group was statistically significant(P <0.05). Compared with the model group, the difference in the body mass between the control group and the treatment group was statistically significant(P<0.05), but there was no significant difference in the body mass between the control group and the treatment group(P >0.05).Compared with the model group, the distal tubules of the control group were arranged more tightly, and there was a little inflammatory exudation between the tissues. And those in the treatment group was less sever than those in the control group. After4 weeks of treatment, the levels of urinary protein, creatinine, urea nitrogen and uric acid in the treatment group were significantly decreased than those before the treatment(P<0.05). Compared with the model group, there were significant differences in the treatment group(P<0.05), and those in the treatment group was statistically significant from those in the model group(P<0.05).Conclusion The Crucian Carp and Chinese Wax Gourd Peel Soup combined with routine treatment can effectively improve the body mass index of rats with nephrotic syndrome and reduce their levels of 24 h urinary protein, creatinine, urea nitrogen and uric acid.
Objective To observe the protective effect of Crucian Carp and Chinese Wax Gourd Peel Soup combined with routine treatment on renal function in nephrotic syndrome rats. Methods A total of 96 SD rats were randomly divided into4 groups: a normal group, a model group, a treatment group and a control group. The control group was given routine treatment.Except the routine treatment, the treatment group was also given Crucian Carp and Chinese Wax Gourd Peel Soup according to the clinical equivalent dosage. Continuous 14 d as 1 course of treatment, with a total of 2 courses of treatment. The pathomorphology of renal tissue, and the levels of 24 h urinary protein, creatinine, urea nitrogen and uric acid were measured before the treatment, 2 weeks after the treatment and 4 weeks after the treatment. Results Compared with the body mass before the treatment,the difference between the control group and the treatment group was statistically significant(P <0.05). Compared with the model group, the difference in the body mass between the control group and the treatment group was statistically significant(P<0.05), but there was no significant difference in the body mass between the control group and the treatment group(P >0.05).Compared with the model group, the distal tubules of the control group were arranged more tightly, and there was a little inflammatory exudation between the tissues. And those in the treatment group was less sever than those in the control group. After4 weeks of treatment, the levels of urinary protein, creatinine, urea nitrogen and uric acid in the treatment group were significantly decreased than those before the treatment(P<0.05). Compared with the model group, there were significant differences in the treatment group(P<0.05), and those in the treatment group was statistically significant from those in the model group(P<0.05).Conclusion The Crucian Carp and Chinese Wax Gourd Peel Soup combined with routine treatment can effectively improve the body mass index of rats with nephrotic syndrome and reduce their levels of 24 h urinary protein, creatinine, urea nitrogen and uric acid.
引文
[1] MARGARET D, SHASHANK J, NICHOLAS H, et al. Albumin and furosemide combination for management of edema in nephrotic syndrome:A review of clinical studies[J]. Cells, 2015, 4(4):622-630.
[2] SULOWICZ W, STOMPOR T. LDL-apheresis and immunoadsorption:novel methods in the treatment of renal diseases re fractory to conventional therapy[J]. Nephrol Dial Transplant, 2003,18(S5):v59-v62.
[3]黄宇新,赵雄,胡仕畅,等.肾病综合征中西医结合治疗进展[J].辽宁中医药大学学报,2015,6(2):219-221.
[4]国家中医药管理局.中医病证诊断疗效标准[M].南京:南京大学出版社,2010:51-52.
[5]郑筱萸.中药新药临床研究指导原则(试行)[M].北京:中国医药科技出版社,2012:22-26.
[6]邹燕勤,王钢.孟河医派临床大家邹云翔论治肾病经验[J].江苏中医药,2016,48(6):1-5.
[7]刘涛.原发性肾病综合征患者74例的临床治疗分析[J].临床医药文献电子杂志,2015,2(14):2713-2714.
[8]张雨,刘缓,闫丽.糖皮质激素与环磷酰胺联合用于肾病综合征治疗的效果观察[J].当代医学,2019,25(8):163-164.
[9]代景贤.原发性肾病综合征50例的中医内科治疗分析[J].中国社区医师,2015,21(23):93-94.
[10] DUFFY M, JAIN S, HARRELL N, et al. Albumin and Furosemide Combination for Management of Edema in Nephrotic Syndrome:A Review of Clinical Studies[J]. Cells,2015,4(4):622-630.
[11]张秋,张昱.张景岳治疗水肿特点探析[J].北京中医药,2010,29(10):764-765.
[12]明·缪希雍.本草经疏[M].上海:中国医药科技出版社,2015(5):25-28.
[13]贾丽魏,张志敏.肾病综合征患者中西医结合饮食护理研究现状[J].中医临床研究,2015,10(7):117-119.
[14]陈秀芹.饮食营养干预在肾病综合征患者护理中的疗效评估[J].中国卫生标准管理,2015,8(10):148-149.
[15]杨芳,何泽云.何泽云教授治疗慢性肾炎蛋白尿经验撷萃[J].湖南中医药大学学报,2015,35(9):42-44.
[2] SULOWICZ W, STOMPOR T. LDL-apheresis and immunoadsorption:novel methods in the treatment of renal diseases re fractory to conventional therapy[J]. Nephrol Dial Transplant, 2003,18(S5):v59-v62.
[3]黄宇新,赵雄,胡仕畅,等.肾病综合征中西医结合治疗进展[J].辽宁中医药大学学报,2015,6(2):219-221.
[4]国家中医药管理局.中医病证诊断疗效标准[M].南京:南京大学出版社,2010:51-52.
[5]郑筱萸.中药新药临床研究指导原则(试行)[M].北京:中国医药科技出版社,2012:22-26.
[6]邹燕勤,王钢.孟河医派临床大家邹云翔论治肾病经验[J].江苏中医药,2016,48(6):1-5.
[7]刘涛.原发性肾病综合征患者74例的临床治疗分析[J].临床医药文献电子杂志,2015,2(14):2713-2714.
[8]张雨,刘缓,闫丽.糖皮质激素与环磷酰胺联合用于肾病综合征治疗的效果观察[J].当代医学,2019,25(8):163-164.
[9]代景贤.原发性肾病综合征50例的中医内科治疗分析[J].中国社区医师,2015,21(23):93-94.
[10] DUFFY M, JAIN S, HARRELL N, et al. Albumin and Furosemide Combination for Management of Edema in Nephrotic Syndrome:A Review of Clinical Studies[J]. Cells,2015,4(4):622-630.
[11]张秋,张昱.张景岳治疗水肿特点探析[J].北京中医药,2010,29(10):764-765.
[12]明·缪希雍.本草经疏[M].上海:中国医药科技出版社,2015(5):25-28.
[13]贾丽魏,张志敏.肾病综合征患者中西医结合饮食护理研究现状[J].中医临床研究,2015,10(7):117-119.
[14]陈秀芹.饮食营养干预在肾病综合征患者护理中的疗效评估[J].中国卫生标准管理,2015,8(10):148-149.
[15]杨芳,何泽云.何泽云教授治疗慢性肾炎蛋白尿经验撷萃[J].湖南中医药大学学报,2015,35(9):42-44.