苦参碱衍生物抗关节炎作用研究
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摘要
目的研究苦参碱衍生物M19对二型胶原诱导性大鼠关节炎(CIA)的治疗作用。方法将60只大鼠随机分为6组,分别为空白组,模型组,吲哚美辛治疗组,M19低、中、高剂量(2.5、5、10 mg/kg)组。采用二型胶原诱导建立关节炎模型,分别对大鼠足趾肿胀和体重进行测定并对关节炎进行评分,HE染色观察大鼠关节软骨及滑膜组织病理变化,Micro-CT观察大鼠骨关节组织损伤情况。结果苦参碱衍生物M19各剂量组均能不同程度降低大鼠关节炎足趾肿胀程度,增加体重,抑制大鼠关节炎性细胞浸润和滑膜增生,并对骨组织具有一定的保护作用。结论苦参碱衍生物M19对二型胶原诱导的关节炎具有一定的治疗作用。
Objective To study the therapeutic effects of matrine derivative M19 on type Ⅱcollagen-induced arthritis(CIA) in rats.Methods Sixty rats were randomly divided into six groups:blank group,model group,indomethacin treatment group,M19 low,medium and high dose groups(2.5,5,10 mg/kg).The paw swelling,arthritis index(AI) and body weight were measured for CIA rats.The histopathological changes of joints and synovial tissue were observed by HE staining.The bone destruction was observed with micro-CT.Results Matrine derivative M19 reduced the paw swelling,increased body weight,inhibited inflammatory cell infiltration,ameliorated synovial tissue and bone destruction in CIA rats.Conclusion Matrine derivative M19 has the certain therapeutic effects on CIA.
Objective To study the therapeutic effects of matrine derivative M19 on type Ⅱcollagen-induced arthritis(CIA) in rats.Methods Sixty rats were randomly divided into six groups:blank group,model group,indomethacin treatment group,M19 low,medium and high dose groups(2.5,5,10 mg/kg).The paw swelling,arthritis index(AI) and body weight were measured for CIA rats.The histopathological changes of joints and synovial tissue were observed by HE staining.The bone destruction was observed with micro-CT.Results Matrine derivative M19 reduced the paw swelling,increased body weight,inhibited inflammatory cell infiltration,ameliorated synovial tissue and bone destruction in CIA rats.Conclusion Matrine derivative M19 has the certain therapeutic effects on CIA.
引文
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[15] 倪晨旭,齐阳,许维恒,等.苦参碱衍生物M19对于肿瘤细胞转移的抑制作用[J].现代肿瘤医学,2016,24(21):3341-3344.
[16] CHEN X,ZHI X,CAO L,et al.Matrine derivate MASM uncovers a novel function for ribosomal protein S5 in osteoclastogenesis and postmenopausal osteoporosis [J].Cell Death Dis,2017,8(9):e3037.
[17] XU J,QI Y,XU W H,et al.Matrine derivate MASM suppresses LPS-induced phenotypic and functional maturation of murine bone marrow-derived dendritic cells [J].Int Immunopharmacol,2016,36:59-66.
[2] BARTOK B,FIRESTEIN G S.Fibroblast-like synoviocytes:key effector cells in rheumatoid arthritis [J].Immunol Rev,2010,233(1):233-255.
[3] KIM S C,SOLOMON D H,LIU J,et al.Risk of venous thromboembolism in patients with rheumatoid arthritis:initiating disease-modifying antirheumatic drugs [J].Am J Med,2015,128(5):539.e7-17.
[4] LIU H M,WANG K J.Therapeutic effect of Captopril on rheumatoid arthritis in rats [J].Asian Pac J Trop Med,2014,7(12):996-999.
[5] 周静,张弦,钱海兵.类风湿性关节炎发病机制的研究进展[J].贵阳中医学院学报,2014,36(5):44-47.
[6] 梁燕,邹豪.类风湿性关节炎药物治疗研究进展[J].海军医学杂志,2014,35(4):332-333.
[7] LIU N,KAN Q C,ZHANG X J,et al.Upregulation of immunomodulatory molecules by matrine treatment in experimental autoimmune encephalomyelitis [J].Exp Mol Pathol,2014,97(3):470-476.
[8] SUN N,WANG Z W,WU C H,et al.Antiviral activity and underlying molecular mechanisms of matrine against porcine reproductive and respiratory syndrome virus in vitro [J].Res Vet Sci,2014,96(2):323-327.
[9] WANG Y,ZHANG S,LIU J,et al.Matrine inhibits the invasive and migratory properties of human hepatocellular carcinoma by regulating epithelial-mesenchymal transition [J].Mol Med Rep,2018,18(1):911-919.
[10] KAN Q C,LV P,ZHANG X J,et al.Matrine protects neuro-axon from CNS inflammation-induced injury [J].Exp Mol Pathol,2015,98(1):124-130.
[11] HU H,WANG S,ZHANG C,et al.Synthesis and in vitro inhibitory activity of matrine derivatives towards pro-inflammatory cytokines [J].Bioorg Med Chem Lett,2010,20(24):7537-7539.
[12] XU W H,HU H G,TIAN Y,et al.Bioactive compound reveals a novel function for ribosomal protein S5 in hepatic stellate cell activation and hepatic fibrosis [J].Hepatology,2014,60(2):648-660.
[13] XU J,WANG K Q,XU W H,et al.The matrine derivate MASM prolongs survival,attenuates inflammation,and reduces organ injury in murine established lethal sepsis [J].J Infect Dis,2016,214(11):1762-1772.
[14] LIU Y,QI Y,BAI Z H,et al.A novel matrine derivate inhibits differentiated human hepatoma cells and hepatic cancer stem-like cells by suppressing PI3K/AKT signaling pathways [J].Acta Pharmacol Sin,2017,38(1):120-132.
[15] 倪晨旭,齐阳,许维恒,等.苦参碱衍生物M19对于肿瘤细胞转移的抑制作用[J].现代肿瘤医学,2016,24(21):3341-3344.
[16] CHEN X,ZHI X,CAO L,et al.Matrine derivate MASM uncovers a novel function for ribosomal protein S5 in osteoclastogenesis and postmenopausal osteoporosis [J].Cell Death Dis,2017,8(9):e3037.
[17] XU J,QI Y,XU W H,et al.Matrine derivate MASM suppresses LPS-induced phenotypic and functional maturation of murine bone marrow-derived dendritic cells [J].Int Immunopharmacol,2016,36:59-66.